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1.
Control of ion loading into the xylem has been repeatedly named as a crucial factor determining plant salt tolerance. In this study we further investigate this issue by applying a range of biophysical [the microelectrode ion flux measurement (MIFE) technique for non‐invasive ion flux measurements, the patch clamp technique, membrane potential measurements] and physiological (xylem sap and tissue nutrient analysis, photosynthetic characteristics, stomatal conductance) techniques to barley varieties contrasting in their salt tolerance. We report that restricting Na+ loading into the xylem is not essential for conferring salinity tolerance in barley, with tolerant varieties showing xylem Na+ concentrations at least as high as those of sensitive ones. At the same time, tolerant genotypes are capable of maintaining higher xylem K+/Na+ ratios and efficiently sequester the accumulated Na+ in leaves. The former is achieved by more efficient loading of K+ into the xylem. We argue that the observed increases in xylem K+ and Na+ concentrations in tolerant genotypes are required for efficient osmotic adjustment, needed to support leaf expansion growth. We also provide evidence that K+‐permeable voltage‐sensitive channels are involved in xylem loading and operate in a feedback manner to maintain a constant K+/Na+ ratio in the xylem sap.  相似文献   

2.
Molecular mechanisms of potassium and sodium uptake in plants   总被引:20,自引:0,他引:20  
Potassium (K+) is an essential nutrient and the most abundant cation in plants, whereas the closely related ion sodium (Na+) is toxic to most plants at high millimolar concentrations. K+ deficiency and Na+ toxicity are both major constraints to crop production worldwide. K+ counteracts Na+ stress, while Na+, in turn, can to a certain degree alleviate K+ deficiency. Elucidation of the molecular mechanisms of K+ and Na+ transport is pivotal to the understanding – and eventually engineering – of plant K+ nutrition and Na+ sensitivity. Here we provide an overview on plant K+ transporters with particular emphasis on root K+ and Na+ uptake. Plant K+-permeable cation transporters comprise seven families: Shaker-type K+ channels, `two-pore' K+ channels, cyclic-nucleotide-gated channels, putative K+/H+ antiporters, KUP/HAK/KT transporters, HKT transporters, and LCT1. Candidate genes for Na+ transport are the KUP/HAK/KTs, HKTs, CNGCs, and LCT1. Expression in heterologous systems, localization in plants, and genetic disruption in plants will provide insight into the roles of transporter genes in K+ nutrition and Na+ toxicity.  相似文献   

3.
Salt sensitive (pea) and salt tolerant (barley) species were used to understand the physiological basis of differential salinity tolerance in crops. Pea plants were much more efficient in restoring otherwise depolarized membrane potential thereby effectively decreasing K+ efflux through depolarization‐activated outward rectifying potassium channels. At the same time, pea root apex was 10‐fold more sensitive to physiologically relevant H2O2 concentration and accumulated larger amounts of H2O2 under saline conditions. This resulted in a rapid loss of cell viability in the pea root apex. Barley plants rapidly loaded Na+ into the xylem; this increase was only transient, and xylem and leaf Na+ concentration remained at a steady level for weeks. On the contrary, pea plants restricted xylem Na+ loading during the first few days of treatment but failed to prevent shoot Na+ elevation in the long term. It is concluded that superior salinity tolerance of barley plants compared with pea is conferred by at least three different mechanisms: (1) efficient control of xylem Na+ loading; (2) efficient control of H2O2 accumulation and reduced sensitivity of non‐selective cation channels to H2O2 in the root apex; and (3) higher energy saving efficiency, with less ATP spent to maintain membrane potential under saline conditions.  相似文献   

4.
Na+ and K+ transport in excised soybean roots   总被引:1,自引:0,他引:1  
Uptake, accumulation and xylem transport of K+ and Na+ in excised roots of soybean were investigated by use of a perfusion technique. This technique permitted independent quantification of, on the one hand, entry of ions into the roots and their transport through the cortex to the xylem vessels, and on the other hand reabsorption from the xylem vessels to the neighbouring cells and the external medium. Data are consistent with a low degree of selective uptake of K+ over Na+. However, Na+ depletion of the xylem stream by reabsorption limits, although weakly, its translocation to the shoots. Na+ reabsorbed is for a great part reexcreted into the external medium. The low efficiency of these processes is discussed in relation to the Na+ sensitivity of soybean.  相似文献   

5.
6.

Adaptation to high salinity is achieved by cellular ion homeostasis which involves regulation of toxic sodium ion (Na+) and Chloride ion (Cl) uptake, preventing the transport of these ions to the aerial parts of the plants and vacuolar sequestration of these toxic ions. Ion transporters have long been known to play roles in maintaining ion homeostasis. Na+ enters the cell through various voltage dependent selective and non-selective ion channels. High Na+ concentration in the plasma membrane is balanced either by uptake of potassium ion (K+) by various potassium importing channels, by salt exclusion mechanism or by sequestration of Na+ in the vacuoles. Therefore, the role of high-affinity potassium transporter, the salt overly sensitive pathway, the most well-defined Na+ exclusion pathway that exports Na+ from cell into xylem and tonoplast localized cation transporters that compartmentalizes Na+ in vacuoles need to be studied in detail and applied to make the plant adaptable to saline soil. Knowledge on the regulation of expression of these transporters by the hormones, microRNAs and other non-coding RNAs can be utilized to manipulate the ion transport. Here, we reviewed paradigm of the ion transporters in salt stress signalling pathways from the recent and past studies aiding transformation of basic knowledge into biotechnological applications to generate engineered salt stress tolerant crops.

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7.
8.
All-atom molecular dynamics is used to investigate the transport of Na+ across a 1,2-dioleoyl-sn-glycero-3-phosphocholine lipid bilayer facilitated by a diazacrown hydraphile. Specifically, the free energy of Na+ passing through the bilayer is calculated using the adaptive biasing force method to study the free energy associated with the increase in Na+ transport in the presence of the hydraphile molecule. The results show that water interaction greatly influences Na+ transport through the lipid bilayer as water is pulled through the bilayer with Na+ forming a water channel. The hydraphile causes a reduction in the free energy barrier for the transport of Na+ through the head group part of the lipid bilayer since it complexes the Na+ reducing the necessity for water to be complexed and, therefore, dragged through with Na+, an energetically unfavorable process. The free energy associated with Na+ being desolvated within the bilayer is significantly decreased in the presence of the hydraphile molecule; the hydraphile increases the number of solvation states of Na+ that can be adopted, and this increase in the number of available configurations provides an entropic explanation for the success of the hydraphile.  相似文献   

9.
Potassium Translocation into the Root Xylem   总被引:9,自引:0,他引:9  
Abstract: Potassium is the most abundant cation in cells of higher plants and plays vital roles in plant growth and develop ment. Since the soil is the only source of potassium, plant roots are well adapted to exploit the soil for potassium and supply it to the leaves. Transport across the root can be divided into three stages: uptake into the root symplast, transport across the symplast and release into the xylem. Uptake kinetics of potassium have been studied extensively in the past and sug gested the presence of high and low affinity systems. Molecular and electrophysiological techniques have now confirmed the existence of discrete transporters encoded by a number of genes. Surprisingly, detailed characterisation of the transpor ters using reverse genetics and heterologous expression shows that a number of the transporters (AKT and AtKUP family) func tion both in the low (μM) and high (mM) K+ range. Electrophy siological studies indicate that K+ uptake by roots is coupled to H+, to drive uptake from micromolar K+. However, thus far only Na+ coupled K+ transport has been demonstrated (HKT1). Ion channels play a major role in the exchange of potassium be tween the symplast and the xylem. An outward rectifying chan nel (KORC) mediates potassium release. Cloning of the gene en coding this channel (SKOR) shows that it belongs to the Shaker super-family. Both electrophysiological and genetic studies demonstrate that K+ release through this channel is controlled by the stress hormone abscisic acid. Interestingly, xylem par enchyma cells of young barley roots also contain a number of in ward rectifying K+ channels that are controlled by G-proteins. The involvement of G-proteins emphasises once more that po tassium transport at the symplast/xylem boundary is under hor monal control. The role of the electrical potential difference across the symplastxylem boundary in controlling potassium release is discussed.  相似文献   

10.
The aim of this study was to determine the range of NaCl concentrations in the nutrient solution that allow Suaeda altissima (L.) Pall., a salt-accumulating halophyte, to maintain the upward gradient of water potential in the “medium-root-leaf” system. We evaluated the contribution of Na+ ions in the formation of water potential gradient and demonstrated that Na+ loading into the xylem is involved in this process. Plants were grown in water culture at NaCl concentrations ranging from zero to 1 M. The water potential of leaf and root cells was measured with the method of isopiestic thermocouple psychrometry. When NaCl concentration in the growth medium was raised in the range of 0–500 mM (the medium water potential was lowered accordingly), the root and leaf cells of S. altissima decreased their water potential, thus promoting the maintenance of the upward water potential gradient in the medium-root-leaf system. Growing S. altissima at NaCl concentrations f 750 mM and 1 M disordered water homeostasis and abolished the upward gradient of water potential between roots and leaves. At NaCl concentrations of 0–250 mM, the detached roots of S. altissima were capable of producing the xylem exudate. The concentration of Na+ in the exudate was 1.3 to 1.6 times higher than in the nutrient medium; the exudate pH was acidic and was lowered from 5.5 to 4.5 with the rise in the salt concentration. The results indicate that the long-distance Na+ transport and, especially, the mechanism of Na+ loading into the xylem play a substantial role in the formation of water potential gradient in S. altissima. The accumulation of Na+ in the xylem and acidic pH values of the xylem sap suggest that Na+ loading into the xylem is carried out by the Na+/H+ antiporter of the plasma membrane in parenchymal cells of the root stele.__________Translated from Fiziologiya Rastenii, Vol. 52, No. 4, 2005, pp. 549–557.Original Russian Text Copyright © 2005 by Balnokin, Kotov, Myasoedov, Khailova, Kurkova, Lun’kov, Kotova.  相似文献   

11.
Improving crop plants to be productive in saline soils or under irrigation with saline water would be an important technological advance in overcoming the food and freshwater crises that threaten the world population. However, even if the transformation of a glycophyte into a plant that thrives under seawater irrigation was biologically feasible, current knowledge about Na+ effects would be insufficient to support this technical advance. Intriguingly, crucial details about Na+ uptake and its function in the plant have not yet been well established. We here propose that under saline conditions two nitrate‐dependent transport systems in series that take up and load Na+ into the xylem constitute the major pathway for the accumulation of Na+ in Arabidopsis shoots; this pathway can also function with chloride at high concentrations. In nrt1.1 nitrate transport mutants, plant Na+ accumulation was partially defective, which suggests that NRT1.1 either partially mediates or modulates the nitrate‐dependent Na+ transport. Arabidopsis plants exposed to an osmotic potential of ?1.0 MPa (400 mOsm) for 24 h showed high water loss and wilting in sorbitol or Na/MES, where Na+ could not be accumulated. In contrast, in NaCl the plants that accumulated Na+ lost a low amount of water, and only suffered transitory wilting. We discuss that in Arabidopsis plants exposed to high NaCl concentrations, root Na+ uptake and tissue accumulation fulfil the primary function of osmotic adjustment, even if these processes lead to long‐term toxicity.  相似文献   

12.
Ionic mechanisms of salt stress perception were investigated by non‐invasive measurements of net H+, K+, Ca2+, Na+, and Cl? fluxes from leaf mesophyll of broad bean (Vicia faba L.) plants using vibrating ion‐selective microelectrodes (the MIFE technique). Treatment with 90 m M NaCl led to a significant increase in the net K+ efflux and enhanced activity of the plasma membrane H+‐pump. Both these events were effectively prevented by high (10 m M ) Ca2+ concentrations in the bath. At the same time, no significant difference in the net Na+ flux has been found between low‐ and high‐calcium treatments. It is likely that plasma membrane K+ and H+ transporters, but not the VIC channels, play the key role in the amelioration of negative salt effects by Ca2+ in the bean mesophyll. Experiments with isotonic mannitol application showed that cell ionic responses to hyperosmotic treatment are highly stress‐specific. The most striking difference in response was shown by K+ fluxes, which varied from an increased net K+ efflux (NaCl treatment) to a net K+ influx (mannitol treatment). It is concluded that different ionic mechanisms are involved in the perception of the ‘ionic’ and ‘osmotic’ components of salt stress.  相似文献   

13.
Control of xylem Na+ loading has often been named as the essential component of salinity tolerance mechanism. However, it is less clear to what extent the difference in this trait may determine differential salinity tolerance between species. In this study, barley (Hordeum vulgare L. cv. CM72) and rice (Oryza sativa L. cv. Dongjin) plants were grown under two levels of salinity. Na+ and K+ concentrations in the xylem sap, and shoot and root tissues were measured at different time points after stress onset. Salt‐exposed rice plants prevented xylem Na+ loading for several days, but failed to control this process in the longer term, ultimately resulting in a massive Na+ shoot loading. Barley plants quickly increased xylem Na+ concentration and its delivery to the shoot (most likely for the purpose of osmotic adjustment) but were able to reduce this process later on, keeping most of accumulated Na+ in the root, thus maintaining non‐toxic shoot Na+ level. Rice plants increased shoot K+ concentration, while barley plants maintained higher root K+ concentration. Control of xylem Na+ loading is remarkably different between rice and barley; this difference may differentiate the extent of the salinity tolerance between species. This trait should be investigated in more detail to be used in the breeding programs aimed to improve salinity tolerance in crops.  相似文献   

14.
The incidence of colorectal cancer has increased annually, and the pathogenesis of this disease requires further investigation. In normal colorectal tissues, ion channels and transporters maintain the water‐electrolyte balance and acid/base homeostasis. However, dysfunction of these ion channels and transporters leads to the development and progression of colorectal cancer. Therefore, this review focuses on the progress in understanding the roles of ion channels and transporters in the colorectum and in colorectal cancer, including aquaporins (AQPs), Cl? channels, Cl?/ exchangers, Na+/ transporters and Na+/H+ exchangers. The goal of this review is to promote the identification of new targets for the treatment and prognosis of colorectal cancer.  相似文献   

15.
Multiple transporters and channels mediate cation transport across the plasma membrane and tonoplast to regulate ionic homeostasis in plant cells. However, much less is known about the molecular function of transporters that facilitate cation transport in other organelles such as Golgi. We report here that Arabidopsis KEA4, KEA5, and KEA6, members of cation/proton antiporters‐2 (CPA2) superfamily were colocalized with the known Golgi marker, SYP32‐mCherry. Although single kea4,5,6 mutants showed similar phenotype as the wild type under various conditions, kea4/5/6 triple mutants showed hypersensitivity to low pH, high K+, and high Na+ and displayed growth defects in darkness, suggesting that these three KEA‐type transporters function redundantly in controlling etiolated seedling growth and ion homeostasis. Detailed analysis indicated that the kea4/5/6 triple mutant exhibited cell wall biosynthesis defect during the rapid etiolated seedling growth and under high K+/Na+ condition. The cell wall‐derived pectin homogalacturonan (GalA)3 partially suppressed the growth defects and ionic toxicity in the kea4/5/6 triple mutants when grown in the dark but not in the light conditions. Together, these data support the hypothesis that the Golgi‐localized KEAs play key roles in the maintenance of ionic and pH homeostasis, thereby facilitating Golgi function in cell wall biosynthesis during rapid etiolated seedling growth and in coping with high K+/Na+ stress.  相似文献   

16.
Transporters and ion channels are conventionally categorised into distinct classes of membrane proteins. However, some membrane proteins have a split personality and can function as both transporters and ion channels. The excitatory amino acid transporters (EAATs) in particular, function as both glutamate transporters and chloride (Cl?) channels. The EAATs couple the transport of glutamate to the co-transport of three Na+ ions and one H+ ion into the cell, and the counter-transport of one K+ ion out of the cell. The EAAT Cl? channel is activated by the binding of glutamate and Na+, but is thermodynamically uncoupled from glutamate transport and involves molecular determinants distinct from those responsible for glutamate transport. Several crystal structures of an EAAT archaeal homologue, GltPh, at different stages of the transport cycle, alongside numerous functional studies and molecular dynamics simulations, have provided extensive insights into the mechanism of substrate transport via these transporters. However, the molecular determinants involved in Cl? permeation, and the mechanism by which this channel is activated are not entirely understood. Here we will discuss what is currently known about the molecular determinants involved in EAAT-mediated Cl? permeation and the mechanisms that underlie their split personality.  相似文献   

17.
Sertoli cells play a key role in the establishment of an adequate luminal environment in the seminiferous tubules of the male reproductive tract. Secretion of the seminiferous tubular fluid (STF) is vital for the normal occurrence of spermatogenesis and for providing a means of transport to the developing spermatozoa. However, several studies on this subject have not completely clarified the origin and composition of this fluid. Electrolyte and water are central components of STF. Sertoli cells secrete an iso-osmotic fluid with a higher content of K+ than the blood and express various membrane and water transporters (Na+/K+-ATPase; Ca2+-ATPase; V-type ATPase; Cl channels; CFTR Cl channels; K+ channels; L-, T- and N-type Ca2+ channels; Na+/H+ exchangers; Na+-driven HCO3 /Cl exchangers (NDCBEs); Na+/HCO3 cotransporters (NBCes); Na+–K+–2Cl cotransporter; Na+/Ca2+ exchanger; and aquaporins 0 and 8) involved in cellular and secretory functions. Studies with knockout mice for some of these transporters showed tubular fluid accumulation and associated infertility, revealing the relevance of these processes for the normal occurrence of spermatogenesis. Nevertheless, the role of the several membrane transporters in the establishment of STF electrolyte composition needs to be further elucidated. This review summarizes the available data on the ionic composition of STF and on the Sertoli cell membrane mechanisms responsible for ion and water movement. Deepening the knowledge on the mechanisms involved in the secretion, composition and regulation of SFT is essential and will be a major step in understanding the infertility associated with some pathological conditions.  相似文献   

18.
Spergularia marina (L.) Griseb. is. a rapidly growing, annual, coastal halophyte. Because of its small size, it is suitable for isotope studies of ion transport well beyond the seedling stage. The purpose of this report is to establish the similarities and differences between 22Na+ and 42K+ uptake in S. marina and in more commonly used mesophytic crop species. Vegetative plants were used 18 days after transfer to solution culture. Plants were grown either on Na+-free medium or on 0.2 × sea water. 22Na+ uptake was linear with time for several hours. The rate was relatively insensitive to external concentration between 1 and 180 mol Na+ m?3, particularly in Na+-free plants. Transport to the shoot accounted for 40 to 70% of the total uptake, dependent on salinity but largely independent of time. 42K+ uptake decreased with increasing salinity in Na+-free plants and increased in 0.2 × sea water plants. Both uptake and transport to the shoot were non-linear with time, upward concavity suggesting recovery from a manipulative and/or osmotic injury. Steady state root contents were compared with predicted contents based on cortical cell electrical potentials using the Nernst equation. Reasonable agreement was found in all cases except Na+ content of 0.2 × sea water plants, in which active efflux was indicated. Uptake studies conducted in the presence of chemical modifiers (dicyclohexylcarbodiimide, dinitrophenol and fusicoccin) showed responses of 42K+ uptake as expected from studies on agronomic species, and implied the presence of a similar active uptake here despite the appearance of equilibrium. Active Na+ uptake was suggested at low Na+ levels. We conclude that S. marina is a promising experimental system combining the rapid nutrient acquisition strategy of agionomically important annuals with a high degree of salt tolerance.  相似文献   

19.
Galdieria sulphuraria and Cyanidioschyzon merolae are thermo‐acidophilic unicellular red algal cousins capable of living in volcanic environments, although the former can additionally thrive in the presence of toxic heavy metals. Bioinformatic analyses of transport systems were carried out on their genomes, as well as that of the mesophilic multicellular red alga Chondrus crispus (Irish moss). We identified transport proteins related to the metabolic capabilities, physiological properties, and environmental adaptations of these organisms. Of note is the vast array of transporters encoded in G. sulphuraria capable of importing a variety of carbon sources, particularly sugars and amino acids, while C. merolae and C. crispus have relatively few such proteins. Chondrus crispus may prefer short chain acids to sugars and amino acids. In addition, the number of encoded proteins pertaining to heavy metal ion transport is highest in G. sulphuraria and lowest in C. crispus. All three organisms preferentially utilize secondary carriers over primary active transporters, suggesting that their primary source of energy derives from electron flow rather than substrate‐level phosphorylation. Surprisingly, the percentage of inorganic ion transporters encoded in C. merolae more closely resembles that of C. crispus than G. sulphuraria, but only C. crispus appears to signal via voltage‐gated cation channels and possess a Na+/K+‐ATPase and a Na+ exporting pyrophosphatase. The results presented in this report further our understanding of the metabolic potential and toxic compound resistances of these three organisms.  相似文献   

20.
Amino acid transport is facilitated by specific transporters within the plasma membrane of the cell. Mediated Na+-independent transport of L-glutamate can be easily detected in mouse oocytes, but it is nearly undetectable in blastocyst-stage embryos. In contrast, the Na+-dependent transport of L-aspartate is not detectable in oocytes, but it is detectable in eight-cell embryos and reaches relatively high levels by the blastocyst stage. It is believed that the amino acid transporters responsible are systems x?c and X?AG, respectively. Here we report the detection of Na+-dependent L-aspartate transport, which increased as pig blastocysts developed, although Na+-dependent aspartate transport was not detected in pig oocytes. Mediated Na+-independent L-glutamate transport was not detected in pig oocytes, in contrast to the mouse, nor in early or hatched pig blastocysts. Thus, while the developmental regulation of system X?AG is similar in both the pig and the mouse, system x?c was not detectable in pig oocytes or blastocysts. Elucidation of the molecular mechanisms controlling amino acid transport and other gene expression in early embryos should contribute to an understanding of whether and even why some aspects of developmental regulation of gene expression may need to differ among species. © 1993 Wiley-Liss, Inc.  相似文献   

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