花生衰老进程的研究

通过对鲁花11号和辐8707 2个高产花生品种的衰老进程研究表明：花生衰老具有地上部（叶片）渐进衰老和整株衰老的双重特点。花生从始花至花后60d左右为地上部（叶片）渐进衰老期：此期主茎高、侧枝长、分枝数、主茎、侧枝绿叶数、叶面积、茎、叶干重迅速增加,并接近或达到最大值,主茎及侧枝基部叶片逐渐由下向上开始衰老死亡,饱果开始出现,根系活力、固氮酶活性逐渐升高至接近最大值,始花后60－90d为整株缓衰期,此期地上部茎叶生长基因停止,逐渐开始衰老死亡,主茎、侧枝绿叶数开始减少,生殖体（荚果）干重迅速增长,根系活力、固氮酶活性缓慢降低;始花后90d以后称为整株速衰期,此期主茎、侧枝绿叶迅速减少,地上部迅速衰老死亡,生殖体（荚果）干重缓慢增长,根系活力、固氮酶活性迅速降低。地上部（叶片）渐进衰老期与开花及大量荚果形成相对应,整株缓衰期伴随着荚果迅速增重,整株速衰期与荚果缓慢增重一致。     

Boron mobility in peanut (Arachis hypogaea L.)

In most plant families, boron (B) is phloem immobile. For plants such as peanut which bury their fruit, the mechanism for B delivery and the B source for fruit and seed growth remains enigmatic. Therefore, this study aimed to establish evidence of B retranslocation in peanut and to identify its importance in plant development. In a sand culture experiment, the increase in B contents in new organs after B withdrawal and the corresponding decline in B contents in older organs was evidence of B redistribution. In a foliar ¹⁰B experiment, the ¹⁰B abundance of treated-leaves decreased and ¹⁰B was detected in leaves and flowers formed after the application of foliar B. Application of ¹⁰B to the roots for a period also provided evidence for the retranslocation of ¹⁰B accumulated during the first growth period. The ¹⁰B abundance in older plant parts declined and ¹⁰B appeared in new organs (flowers, pegs, leaves) that had developed after the ¹⁰B supply had been replaced by ¹¹B. In the fourth experiment, foliar application of B reduced hollow heart, a symptom of B deficiency in seeds, in cv. TAG 24 from 39 to 8% and in Tainan 9 from 63 to 18%. These experiments all provide evidence for B retranslocation in peanut, but further work on the relative importance of the xylem and phloem pathways for B loading into the fruit is needed.     

花生分子标记的研究进展

国内外对花生的研究特别是在分子水平上的研究相对水稻、油菜等农作物比较薄弱。近些年,分子标记技术迅速发展,在花生上也得到广泛的应用。本文从花生属起源、种质资源的遗传多样性、抗性基因的标记和指纹图谱等方面,综述了国内外花生分子标记的研究进展。     

Pterocarpenes elicited by Aspergillus caelatus in peanut (Arachis hypogaea) seeds

The substituted pterocarpenes named aracarpene-1 (1) and aracarpene-2 (2) were isolated from wounded peanut seeds challenged by a strain of Aspergillus caelatus. The structures of these putative phytoalexins were determined by interpretation of NMR and MS data. The aracarpenes were investigated for their antifungal and antibacterial activities as well as antioxidant, anti-inflammatory, and cytotoxic activities in mammalian cells. Aracarpene-2 demonstrated high antibacterial properties against tested gram-positive and gram-negative bacteria, whereas aracarpene-1 displayed low antibacterial properties against the same bacteria. Both compounds had no antifungal activity against Aspergillus flavus. Together with peanut stilbenoids that are also produced in the challenged seeds, these compounds may represent a class of low-molecular weight peanut metabolites with a defensive role(s) against pathogenic microorganisms.     

Immunochemical studies on the specificity of the peanut (Arachis hypogaea) agglutinin.

The specificity of purified, peanut agglutinin has been studied immunochemically by quantitative precipitin and inhibition assays. The lectin showed substantial differences in precipitating with blood-group substances of the same specificity. Of the B substances tested, horse 4 25% completely precipitated the lectin, Beach phenol insoluble failed to interact, and PM phenol insoluble gave an intermediate reaction. The lectin did not precipitate with A1 substances, with hog gastric mucin A + H substance, or with A2 substance WG phenol insoluble. Another A2 substance, cyst 14 phenol insoluble, precipitated approximately 2/3 of the lectin. Of the H substances, Tighe phenol insoluble was inactive, JS phenol insoluble precipitated poorly, and morgan standard H precipitated about 80% of the lectin. However, first stage of Smith degradation, as well as Pl fractions obtained by mild acid hydrolysis of blood-group substances, gave products which precipitated strongly. The lectin was also completely precipitated by all precursor blood-group substances, as well as by cows 21 and 26, all having strong I-Ma, I-Ort, I-Step, and I-Da activities. Cow 18, which does not possess significant blood-group I activity, precipitated very slightly. Fractions of blood-group substances N-1 (Lea) and Tij (B) obtained by precipitation from 90 percent phenol at higher concentrations of ethanol interacted better with peanut agglutinin. These differences in activity are ascribable to a heterogeneity resulting from incomplete biosynthesis of carbohydrate side-chains of blood-group substances, particularly resulting in variations in the numbers of DGalbeta1 leads to 3DGalNAc or DGalbeta1 leads to 4DGlcNAc determinants. The agglutinin reacted with the hydatid cyst P1 glycoprotein, as well as with the previously studied antifreeze and sialic acid-free alpha1 acid glycoproteins, but not with pneumococcus type XIV polysaccharide. Inhibition of precipitation showed the lectin to be most specific for the disaccharide DGalbeta1 leads to 3DGalNAc, which is 14, 55, and 90 times as active as DGalbeta1 leads to 4DGlcNAc, DGal, and DGalbeta1 leads to 3DGlcNAc, respectively. DGalbeta1 leads to 3N-acetyl-D-galactosaminitol has approximately 1/25th the activity of DGalbeta1 leads to 3DGalNAc. Substitutions of DGlcNAc or LFuc on the DGal of active inhibitors completely blocked the activity, in line with the assumption that the combining site of the peanut lectin is a partial cavity. The oligosaccharides DGalbeta1 leads to 4DGlcNAcbeta1 leads to 6-hexane-1,2,4,5,6-pentol(s) and DGalbeta1 leads to 3[DGalbeta1 leads to 4DGlcNAcbeta1 leads to 6]N-acetyl-D-galactosaminitol showed the same inhibitory activity as DGalbeta1 leads to 4DGlcNAc, suggesting that the combining site of the peanut agglutinin may not be complementary to more than a disaccharide...     

A simplified methodology for purification of peanut (Arachis hypogaea) agglutinin

The agglutinin from peanut (Arachis hypogaea) was readily isolated by affinity chromatography on acid-treated Sepharose 6B. The recovered lectin (50 mg/100 g seeds) appeared as a single band of Mr 32,000 on gel electrophoresis and its specific haemagglutination titre on desialylated human A red blood cells was very high (2(15)).     

Importance of glutathione in the nodulation process of peanut (Arachis hypogaea)

GSH appears to be essential for proper development of the root nodules during the symbiotic association of legume-rhizobia in which the entry of rhizobia involves the formation of infection threads. In the particular case of peanut-rhizobia symbiosis, the entry of rhizobia occurs by the mechanism of infection called 'crack entry', i.e. entry at the point of emergence of lateral roots. We have previously shown the role of GSH content of Bradyrhizobium sp. SEMIA 6144 during the symbiotic association with peanut using a GSH-deficient mutant obtained by disruption of the gshA gene, encoding gamma-glutamylcysteine synthetase (gamma-GCS), which was able to induce nodules in peanut roots without alterations in the symbiotic phenotype. To investigate the role of the peanut GSH content in the symbiosis, the compound L-buthionine-sulfoximine (BSO), a specific inhibitor of gamma-GCS in plants, was used. There were no differences in the plant growth and the typical anatomic structure of the peanut roots when the plants grew in the Fahraeus medium either in presence or in absence of 0.1 mM BSO. However, the GSH content was reduced by 51% after treatment with BSO. The BSO-treated plants inoculated with wild-type or mutant strains of Bradyrhizobium sp. showed a significant reduction in the number and dry weight of nodules, suggesting that GSH content could play an important role in the nodulation process of root peanut with Bradyrhizobium sp.     

Role of endogenous purine metabolism in thidiazuron-induced somatic embryogenesis of peanut (Arachis hypogaea L.)

Somatic embryogenesis was induced at the hypocotyledonary notch region of intact peanut (Arachis hypogaea L.) seedings cultured on a medium containing 10 mol·L^–1 thidiazuron (TDZ). Inclusion of the purine analogs 2,6-diaminopurine (DAP), azaadenine or azaguanine to the thidiazuron amended medium inhibited the embryogenic response of the seedlings. DAP-mediated inhibition was not overcome by the addition of adenine sulphate. Inhibition of the embryogenic response by DAP provides evidence that the TDZ-induced accumulation of purine cytokinins is an essential component of TDZ-induced somatic embryogenesis process. Analyses of the endogenous level of purine metabolites indicated that supplementation of the media with TDZ resulted in an overall increase in the endogenous cytokinins while DAP inhibited the purine recycling resulting in decreased levels of endogenous adenine and zeatin.     

Thidiazuron promotes high frequency regeneration of peanut (Arachis hypogaea) plants in vitro

Multiple shoots were induced on Valenciatype peanut (Arachis hypogaea L.) explants cultured in vitro on a nutrient medium supplemented with thidiazuron. Zygotic embryos excised from mature seeds were germinated on Murashige-Skoog nutrient medium, and the resulting plantlets (8 days-old) were used as a source of explants. When cultured on a nutrient medium with increasing levels of thidiazuron (0.5 to 30 mg/l), expiants from various parts of the peanut plant (except the root) produced multiple shoot primordia which subsequently developed into individual shoots. Hypocotyl and cotyledon explants produced shoots in higher numbers than other explants (20 shoots per hypocotyl explant at all thidiazuron concentrations and 15 shoots per cotyledon explant at 30 mg/l). Shoots rooted normally on a basal Murashige-Skoog medium containing charcoal and developed into healthy and fertile plants when planted in soil.Abbreviations TDZ thidiazuron - MSO Murashige and Skoog (1962) basal medium - BA 6-benzylaminopurine     

Proteomics reveals elevated levels of PR 10 proteins in saline-tolerant peanut (Arachis hypogaea) calli.

The proteome of a salinity-tolerant Arachis hypogaea L. callus cell line was compared with its sensitive counterpart. Several low molecular weight proteins were detected by two-dimensional electrophoresis as being unique or significantly elevated in the tolerant line. The identities of several of these proteins were established as PR 10 proteins using tandem Mass Spectrometry and are shown to be phosphorylated on the basis of staining with the phosphorylation-specific stain, Pro-Q Diamond. Our results suggest that these differentially phosphorylated PR 10 proteins may play an important role in mediating salinity stress responses.     

Utility of EST-derived SSR in cultivated peanut (Arachis hypogaea L.) and Arachis wild species

Background  

Lack of sufficient molecular markers hinders current genetic research in peanuts (Arachis hypogaea L.). It is necessary to develop more molecular markers for potential use in peanut genetic research. With the development of peanut EST projects, a vast amount of available EST sequence data has been generated. These data offered an opportunity to identify SSR in ESTs by data mining.     

花生镉污染研究进展

花生既是世界主要的油料作物,又是重要的植物蛋白来源和食品加工原料.随着花生直接食用和食品加工的不断增加,国际上对花生籽粒Cd含量问题越来越关注.我国是世界上重要的花生生产国和出口国.近年来,花生Cd含量偏高已经成为制约我国出口贸易的重要因素.本文从花生籽粒Cd富集能力、花生Cd含量的种内差异、籽粒中Cd的分布规律、影响花生籽粒Cd积累的机制和降低花生籽粒Cd含量技术等方面,对花生Cd污染研究的现状与问题进行了论述.指出在花生Cd污染控制方面有2种策略可以考虑,一是降低花生对土壤Cd的吸收;二是控制Cd向籽粒的迁移富集.为此需要从3个方面加强对花生籽粒Cd积累机制的研究,即花生根系活性特征参数及其与籽粒Cd积累的关系;花生果荚Cd吸收机制及其对籽粒Cd含量的贡献;花生植株体内Cd迁移机制及其与籽粒Cd含量的关系.     

Fungal populations in the rhizosphere of peanut (Arachis hypogaea L.)

Summary A comparison of fungal populations in the rhizospheres of eight varieties of peanut grown in a red lateritic soil amended with farmyard manure was made by the dilution-plate technique. There was a marked increase in fungi in the rhizospheres of TMV 2, TMV 4, Pollachi Red and EC 1698, the increase was smaller in Spanish Improved and RS 1 while very little rhizosphere effect was shown by TMV 3 and Pondicherry 8. Age of the plant had a significant influence on numbers of fungi in the rhizosphere. High R/S ratios were obtained when the plants were 30 days old, at which time attained maximum vegetative growth and started to flower. The ratios gradually decreased after that age until the plants were three months old when there was again a small increase. This later rise in fungal populations is interpreted to be due to an increase in microbial activity around dead or senescent roots. No correlation could be established between numbers of root nodules produced by a variety and its rhizosphere effect. Preferential stimulation of certain fungi in the rhizosphere of some of the varieties was noticed.     

花生镉污染研究进展

花生既是世界主要的油料作物,又是重要的植物蛋白来源和食品加工原料．随着花生直接食用和食品加工的不断增加,国际上对花生籽粒Cd含量问题越来越关注．我国是世界上重要的花生生产国和出口国．近年来,花生Cd含量偏高已经成为制约我国出口贸易的重要因素．本文从花生籽粒Cd富集能力、花生Cd含量的种内差异、籽粒中Cd的分布规律、影响花生籽粒Cd积累的机制和降低花生籽粒Cd含量技术等方面,对花生Cd污染研究的现状与问题进行了论述．指出在花生cd污染控制方面有2种策略可以考虑,一是降低花生对土壤Cd的吸收;二是控制Cd向籽粒的迁移富集．为此需要从3个方面加强对花生籽粒Cd积累机制的研究,即花生根系活性特征参数及其与籽粒Cd积累的关系;花生果荚Cd吸收机制及其对籽粒Cd含量的贡献;花生植株体内Cd迁移机制及其与籽粒Cd含量的关系．     

Plant regeneration through somatic embryogenesis in peanut (Arachis hypogaea L.)

Somatic embryos were induced from immature cotyledons and immature embryonal axis ofArachis hypogaea L. on L-6 basal medium supplemented with NAA, picloram or 2,4-D at 5–50 mg 1^-1. Immature embryonal axis produced a higher number of somatic embryos in comparison with immature cotyledons. The highest number of responding cultures was produced on medium supplemented with NAA (50 mg 1^-1), while the highest average number of somatic embryos per culture was produced on medium with 2,4-D (10 or 20 mg 1^-1) and picloram (30 mg 1^-1) from cotyledons. The somatic embryos developed into plants on basal medium supplemented with activated charcoal and about 100 plants were successfully transferred to the field. Acknowledgement: The authors wish to thank Nuclear Agriculture Division, BARC for supplyingA. hypogaea seeds and Mr. R.M. Mudliar for photography.     

Agrobacterium tumefaciens mediated gene transfer in peanut (Arachis hypogaea L.)

Transgenic peanut plants were produced using Agrobacterium mediated gene transfer. Primary leaf explants of peanut were co-cultivated with Agrobacterium tumefaciens LBA 4404 harbouring the binary plasmid pBI 121 (conferring -glucuronidase activity and resistance to kanamycin) and cultured on regeneration medium supplemented with kanamycin to select putatively transformed shoots. They were rooted and plants were transferred to soil. Stable integration and expression of the transgenes were confirmed by NPT II assay, Southern blot hybridization and GUS assay.Abbreviations BA 6-benzyladenine - GUS -glucuronidase - IAA indole-3-acetic acid - NAA -naphthaleneacetic acid - NOS nopaline synthase - NPT II neomycin phosphotransferase II - SDS Lauryl sulfate     

土壤类型与作物基因型对花生籽实镉积累的影响

以花生主产区的棕壤和潮土为供试土壤,以种植面积最大的白沙1016、花育22和湛油27基因型花生(Arachis hypogaea)为供试作物,采用不添加Cd(对照)和添加Cd(1.5 mg·kg^-1)处理进行盆栽试验,研究土壤类型和作物基因型对花生籽实Cd积累的影响.结果表明：两种土壤对照处理的3种基因型花生籽实Cd含量均低于国家食品安全标准,而Cd处理下均高于食品安全标准. 同种土壤Cd处理3种基因型花生籽实Cd含量显著高于对照,不同基因型间表现为湛油27＞白沙1016＞花育22,棕壤花生籽实Cd含量及总量均高于潮土. 对照处理3种基因型花生籽实Cd生物富集系数均大于1.0,Cd处理下多小于1.0,表明花生籽实对土壤中Cd的累积能力较强,土壤Cd含量进一步增加时,其生物富集能力降低.     

Mass spectrometric characterization of isoform variants of peanut (Arachis hypogaea) stem lectin (SL-I)

Matrix assisted laser desorption/ionization–time-of-flight (MALDI–TOF) mass spectrometric (MS) analysis of purified Arachis hypogaea stem lectin (SL-I) and its tryptic digests suggested it to be an isoformic glucose/mannose binding lectin. Two-dimensional gel electrophoresis of SL-I indicated six isoforms (A1–A6), which were confirmed by Western blotting and MALDI–TOF MS analysis. Comparative analysis of peptide mass spectra of the isoforms matched with A. hypogaea lectins with three different accession numbers (Q43376_ARAHY, Q43377_ARAHY, Q70DJ5_ARAHY). Tandem mass spectrometric (MS/MS) analysis of tryptic peptides revealed these to be isoformic variants with altered amino acid sequences. Among the peptides, the peptide T12 showed major variation. The ¹⁹⁹Val–Ser–Tyr–Asn²⁰² sequence in peptide T12 of A1 and A2 was replaced by ¹⁹⁹Leu–Ser–His–Glu²⁰² in A3 and A4 (T12′) while in A5 and A6 this sequence was ¹⁹⁹Val–Ser–Tyr–Val²⁰² (T12″). Peptide T1 showed the presence of ¹⁰Asn in the isoforms A1–A5 while in A6 this amino acid was replaced by ¹⁰Lys (T1′). Overall amino acid sequence as identified by MS/MS showed a high degree of similarity between A1, A2 and among A3, A4, A5. Carbohydrate binding domain and adenine binding site seem to be conserved.