共查询到20条相似文献,搜索用时 31 毫秒
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Lutz Herrmann Michael Erkelenz Ingo Aldag Arno Tiedtke Marcus WW Hartmann 《BMC microbiology》2006,6(1):19-9
Background
Over the last decades molecular biologic techniques have been developed to alter the genome and proteome of Tetrahymena thermophila thereby providing the basis for recombinant protein expression including functional human enzymes. The biotechnological potential of Tetrahymena has been proved in numerous publications, demonstrating fast growth, high biomass, fermentation in ordinary bacterial/yeast equipment, up-scalability, existence of cheap and chemical defined media. For these reasons Tetrahymena offers promising opportunities for the development of a high expression system. Yet optimised high yield strains with protease deficiency such as commonly used in yeast and bacterial systems are not available. 相似文献3.
Jin-Seung Park Kyung-Yeon Han Jong-Ho Lee Jong-Am Song Keum-Young Ahn Hyuk-Seong Seo Sang-Jun Sim Seung-Wook Kim Jeewon Lee 《BMC biotechnology》2008,8(1):15
Background
The most efficient method for enhancing solubility of recombinant proteins appears to use the fusion expression partners. Although commercial fusion partners including maltose binding protein and glutathione-S-transferase have shown good performance in enhancing the solubility, they cannot be used for the proprietory production of commercially value-added proteins and likely cannot serve as universal helpers to solve all protein solubility and folding issues. Thus, novel fusion partners will continue to be developed through systematic investigations including proteome mining presented in this study. 相似文献4.
Thomas R Burkard Melanie Planyavsky Ines Kaupe Florian P Breitwieser Tilmann Bürckstümmer Keiryn L Bennett Giulio Superti-Furga Jacques Colinge 《BMC systems biology》2011,5(1):17
Background
On the basis of large proteomics datasets measured from seven human cell lines we consider their intersection as an approximation of the human central proteome, which is the set of proteins ubiquitously expressed in all human cells. Composition and properties of the central proteome are investigated through bioinformatics analyses. 相似文献5.
Liv Anette Bøhle Ellen M Færgestad Eva Veiseth-Kent Hilde Steinmoen Ingolf F Nes Vincent GH Eijsink Geir Mathiesen 《Proteome science》2010,8(1):37
Background
Enterococcus faecalis is an opportunistic pathogen and one of the most important causes of hospital infections. Bile acids are a major stress factor bacteria have to cope with in order to colonize and survive in the gastro-intestinal tract. The aim of this study was to investigate the effects of bile acids on the intracellular proteome of E. faecalis V583. 相似文献6.
Carla Cacciotto Maria Filippa Addis Daniela Pagnozzi Bernardo Chessa Elisabetta Coradduzza Laura Carcangiu Sergio Uzzau Alberto Alberti Marco Pittau 《BMC microbiology》2010,10(1):225
Background
Mycoplasmas are the simplest bacteria capable of autonomous replication. Their evolution proceeded from gram-positive bacteria, with the loss of many biosynthetic pathways and of the cell wall. In this work, the liposoluble protein complement of Mycoplasma agalactiae, a minimal bacterial pathogen causing mastitis, polyarthritis, keratoconjunctivitis, and abortion in small ruminants, was subjected to systematic characterization in order to gain insights into its membrane proteome composition. 相似文献7.
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Background
Recent analysis of the human and mouse genomes has shown that a substantial proportion of protein coding genes and cis-regulatory elements contain transposable element (TE) sequences, implicating TE domestication as a mechanism for the origin of genetic novelty. To understand the general role of TE domestication in eukaryotic genome evolution, it is important to assess the acquisition of functional TE sequences by host genomes in a variety of different species, and to understand in greater depth the population dynamics of these mutational events. 相似文献9.
Nelson C Soares Maria P Cabral José R Parreira Carmen Gayoso Maria J Barba Germán Bou 《Proteome science》2009,7(1):37-10
Background
Acinetobacter baumannii is a nosocomial pathogen that has been associated with outbreak infections in hospitals. Despite increasing awareness about this bacterium, its proteome remains poorly characterised, however recently the complete genome of A. baumannii reference strain ATCC 17978 has been sequenced. Here, we have used 2-DE and MALDI-TOF/TOF approach to characterise the proteome of this strain. 相似文献10.
Background
Overexpression of recombinant proteins usually triggers the induction of heat shock proteins that regulate aggregation and solubility of the overexpressed protein. The two-dimensional gel electrophoresis (2-DE)-mass spectrometry approach was used to profile the proteome of Escherichia coli overexpressing N-acetyl-D-glucosamine 2-epimerase (GlcNAc 2-epimerase) and N -acetyl-D-neuraminic acid aldolase (Neu5Ac aldolase), both fused to glutathione S-transferase (GST) and polyionic peptide (5D or 5R). 相似文献11.
Background
Conserved domains (CD) in proteins play a crucial role in protein interactions, DNA binding, enzyme activity, and other important cellular processes. We proposed to study ratios of genes containing these domains to ratios of proteome size of different eukaryotes. 相似文献12.
Gwënaël Pottiez Barbara Deracinois Sophie Duban-Deweer Roméo Cecchelli Laurence Fenart Yannis Karamanos Christophe Flahaut 《Proteome science》2010,8(1):57
Background
Brain capillary endothelial cells (BCECs) form the physiological basis of the blood-brain barrier (BBB). The barrier function is (at least in part) due to well-known proteins such as transporters, tight junctions and metabolic barrier proteins (e.g. monoamine oxidase, gamma glutamyltranspeptidase and P-glycoprotein). Our previous 2-dimensional gel proteome analysis had identified a large number of proteins and revealed the major role of dynamic cytoskeletal remodelling in the differentiation of bovine BCECs. The aim of the present study was to elaborate a reference proteome of Triton X-100-soluble species from bovine BCECs cultured in the well-established in vitro BBB model developed in our laboratory. 相似文献13.
Song Chen Jennifer S Armistead Katie N Provost-Javier Joyce M Sakamoto Jason L Rasgon 《BMC evolutionary biology》2010,10(1):142
Background
Mosquito vitellogenin (Vtg) genes belong to a small multiple gene family that encodes the major yolk protein precursors required for egg production. Multiple Vtg genes have been cloned and characterized from several mosquito species, but their origin and molecular evolution are poorly understood. 相似文献14.
Zlatko Smole Nela Nikolic Fran Supek Tomislav ?muc Ivo F Sbalzarini Anita Krisko 《BMC evolutionary biology》2011,11(1):26
Background
Prokaryotic environmental adaptations occur at different levels within cells to ensure the preservation of genome integrity, proper protein folding and function as well as membrane fluidity. Although specific composition and structure of cellular components suitable for the variety of extreme conditions has already been postulated, a systematic study describing such adaptations has not yet been performed. We therefore explored whether the environmental niche of a prokaryote could be deduced from the sequence of its proteome. Finally, we aimed at finding the precise differences between proteome sequences of prokaryotes from different environments. 相似文献15.
Background
Proteomics is the study of the proteome, and is critical to the understanding of cellular processes. Two central and related tasks of proteomics are protein identification and protein characterization. Many small laboratories are interested in the characterization of a small number of proteins, e.g., how posttranslational modifications change under different conditions. 相似文献16.
Bettina Zeis Tobias Lamkemeyer Rüdiger J Paul Frank Nunes Susanne Schwerin Marita Koch Wolfgang Schütz Johannes Madlung Claudia Fladerer Ralph Pirow 《BMC physiology》2009,9(1):7-18
Background
Freshwater planktonic crustaceans of the genus Daphnia show a remarkable plasticity to cope with environmental changes in oxygen concentration and temperature. One of the key proteins of adaptive gene control in Daphnia pulex under hypoxia is hemoglobin (Hb), which increases in hemolymph concentration by an order of magnitude and shows an enhanced oxygen affinity due to changes in subunit composition. To explore the full spectrum of adaptive protein expression in response to low-oxygen conditions, two-dimensional gel electrophoresis and mass spectrometry were used to analyze the proteome composition of animals acclimated to normoxia (oxygen partial pressure [Po2]: 20 kPa) and hypoxia (Po2: 3 kPa), respectively. 相似文献17.
Bhakti Prinsi Alfredo S Negri Paolo Pesaresi Maurizio Cocucci Luca Espen 《BMC plant biology》2009,9(1):113-17
Background
Nitrogen nutrition is one of the major factors that limit growth and production of crop plants. It affects many processes, such as development, architecture, flowering, senescence and photosynthesis. Although the improvement in technologies for protein study and the widening of gene sequences have made possible the study of the plant proteomes, only limited information on proteome changes occurring in response to nitrogen amount are available up to now. In this work, two-dimensional gel electrophoresis (2-DE) has been used to investigate the protein changes induced by NO3 - concentration in both roots and leaves of maize (Zea mays L.) plants. Moreover, in order to better evaluate the proteomic results, some biochemical and physiological parameters were measured. 相似文献18.
Rafael AP Guércio Anna Shevchenko Andrej Shevchenko Jorge L López-Lozano Jaime Paba Marcelo V Sousa Carlos AO Ricart 《Proteome science》2006,4(1):11-14
Background
Bothrops atrox is responsible for the majority of snakebite accidents in the Brazilian Amazon region. Previous studies have demonstrated that the biological and pharmacological activities of B. atrox venom alter with the age of the animal. Here, we present a comparative proteome analysis of B. atrox venom collected from specimens of three different stages of maturation: juveniles, sub-adults and adults. 相似文献19.