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Immune activation of NF-kappaB and JNK requires Drosophila TAK1 总被引:1,自引:0,他引:1
Silverman N Zhou R Erlich RL Hunter M Bernstein E Schneider D Maniatis T 《The Journal of biological chemistry》2003,278(49):48928-48934
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Two attacin antibacterial genes of Drosophila melanogaster 总被引:7,自引:0,他引:7
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Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Drosophila Imd pathway 总被引:1,自引:0,他引:1
Kleino A Valanne S Ulvila J Kallio J Myllymäki H Enwald H Stöven S Poidevin M Ueda R Hultmark D Lemaitre B Rämet M 《The EMBO journal》2005,24(19):3423-3434
The Imd signaling cascade, similar to the mammalian TNF-receptor pathway, controls antimicrobial peptide expression in Drosophila. We performed a large-scale RNAi screen to identify novel components of the Imd pathway in Drosophila S2 cells. In all, 6713 dsRNAs from an S2 cell-derived cDNA library were analyzed for their effect on Attacin promoter activity in response to Escherichia coli. We identified seven gene products required for the Attacin response in vitro, including two novel Imd pathway components: inhibitor of apoptosis 2 (Iap2) and transforming growth factor-activated kinase 1 (TAK1)-binding protein (TAB). Iap2 is required for antimicrobial peptide response also by the fat body in vivo. Both these factors function downstream of Imd. Neither TAB nor Iap2 is required for Relish cleavage, but may be involved in Relish nuclear localization in vitro, suggesting a novel mode of regulation of the Imd pathway. Our results show that an RNAi-based approach is suitable to identify genes in conserved signaling cascades. 相似文献
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The imd gene is required for local Cecropin expression in Drosophila barrier epithelia 总被引:6,自引:0,他引:6
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Surfaces of higher eukaryotes are normally covered with microorganisms but are usually not infected by them. Innate immunity and the expression of gene-encoded antimicrobial peptides play important roles in the first line of defence in higher animals. The immune response in Drosophila promotes systemic expression of antimicrobial peptides in response to microbial infection. We now demonstrate that the epidermal cells underlying the cuticle of larvae respond to infected wounds by local expression of the genes for the antimicrobial peptide cecropin A. Thus, the Drosophila epidermis plays an active role in the innate defence against microorganisms. The immune deficiency (imd) gene was found to be a crucial component of the signal-induced epidermal expression in both embryos and larvae. In contrast, melanization, which is part of the wound healing process, is not dependent on the imd gene, indicating that the signalling pathways promoting melanization and antimicrobial peptide gene expression can be uncoupled. 相似文献