Cytopathology training in the United States – a personal view

In this review we present an outline of cytopathology training in the United States, for a non-US readership.     

The Holdridge life zones of the conterminous United States in relation to ecosystem mapping

Aim Our main goals were to develop a map of the life zones for the conterminous United States, based on the Holdridge Life Zone system, as a tool for ecosystem mapping, and to compare the map of Holdridge life zones with other global vegetation classification and mapping efforts. Location The area of interest is the forty-eight contiguous states of the United States. Methods We wrote a PERL program for determining life zones from climatic data and linked it to the image processing workbench (IPW). The inputs were annual precipitation (Pann), biotemperature (T_bio), sea-level biotemperature (T₀bio), and the frost line. The spatial resolution chosen for this study (2.5 arc-minute for classification, 4-km for mapping) was driven by the availability of current state-of-the-art, accurate and reliable precipitation data. We used the Precipitation-elevation Regressions on Independent Slopes Model, or PRISM, output for the contiguous United States downloaded from the Internet. The accepted standard data for air temperature surfaces were obtained from the Vegetation/Ecosystem Modelling and Analysis Project (VEMAP). This data set along with station data obtained from the National Climatic Data Center for the US, were used to develop all temperature surfaces at the same resolution as the Pann. Results The US contains thirty-eight life zones (34% of the world's life zones and 85% of the temperate ones) including one boreal, twelve cool temperate, twenty warm temperate, four subtropical, and one tropical. Seventy-four percent of the US falls in the ‘basal belt’, 18% is montane, 8% is subalpine, 1% is alpine, and < 0.1% is nival. The US ranges from superarid to superhumid, and the humid province is the largest (45% of the US). The most extensive life zone is the warm temperate moist forest, which covers 23% of the country. We compared the Holdridge life zone map with output from the BIOME model, Bailey's ecoregions, Küchler potential vegetation, and land cover, all aggregated to four cover classes. Despite differences in the goals and methods for all these classification systems, there was a very good to excellent agreement among them for forests but poor for grasslands, shrublands, and nonvegetated lands. Main conclusions We consider the life zone approach to have many strengths for ecosystem mapping because it is based on climatic driving factors of ecosystem processes and recognizes ecophysiological responses of plants; it is hierarchical and allows for the use of other mapping criteria at the association and successional levels of analysis; it can be expanded or contracted without losing functional continuity among levels of ecological complexity; it is a relatively simple system based on few empirical data; and it uses objective mapping criteria.     

Recommendations for the Implementation of a National Sediment Quality Policy in the United States

This article was prepared prior to the author's employment by the U.S. Environmental Protection Agency and therefore does not necessarily represent agency policy. Sediments provide needed habitats to valued aquatic and marine resources and are therefore an integral and valuable component of any coastal ecosystem. Studies have found that multiple anthropogenic activities have been the primary cause of sediment contamination. There is continued evidence that contaminated sediments pose adverse impacts to biological resources even when overlying water quality is acceptable. Efforts in environmental protection should therefore incorporate sediment quality management strategies. Despite a growing awareness and interest in management of contaminated sediment, no established systematic and standardized national management program has been established in the U.S. The establishment of a national sediment policy in the U.S. would reinforce the importance of integrating sediment issues with different management objectives and reiterate the importance of protecting sediments as a natural resource. A national sediment policy could also guide and direct states in developing state sediment policies that reflect national objectives while integrating local concerns. This perspective identifies and defines issues and concerns raised by 13 states’ agency staff in managing contaminated sediments in the absence of a national policy. Recommendations are developed that address sociopolitical and scientific issues and concerns. The recommendations are integrated with stakeholder comments, state manager needs and the existing regulatory and institutional framework.     

Assessment of streams of the eastern United States using a periphyton index of biotic integrity

Benthic algae were collected from 272 eastern United States streams and rivers and analyzed for diatom species richness and dominance, the relative abundance of acidobiontic, eutraphentic, and motile diatoms, standing crops of chlorophyll and biomass, and alkaline phosphatase activity. These data were used to calculate a periphyton index of biotic integrity (PIBI), and values of the index were compared among reference, moderately impacted, and disturbed streams. The level of disturbance was based on stream chemistry, riparian disturbance, or a combined classification. Analyses of variance showed that PIBI was significantly higher in reference streams for all classifications. The PIBI and its metrics were correlated with many of the chemistry and habitat variables, and canonical correlation analysis revealed three significant environmental gradients which extracted 84% of the variance in the PIBI and its metrics. We used the mean 75th, 25th, and 5th percentile scores from the reference sites to set thresholds for excellent, good, fair, or poor condition. Applying these criteria to the cumulative distribution of total stream length in the region, we found that 4.3% of the stream length was in excellent condition; 20.8% in good condition; 56.4% in fair condition; and 18.5% in poor condition. The sensitivity of the PIBI and its component metrics to environmental stressors supports the use of this index for monitoring ecological conditions in streams in the eastern United States and as a tool to aid in diagnosing the causes of their impairment.     

Wetlands and global climate change: the role of wetland restoration in a changing world

Global climate change is recognized as a threat to species survival and the health of natural systems. Scientists worldwide are looking at the ecological and hydrological impacts resulting from climate change. Climate change will make future efforts to restore and manage wetlands more complex. Wetland systems are vulnerable to changes in quantity and quality of their water supply, and it is expected that climate change will have a pronounced effect on wetlands through alterations in hydrological regimes with great global variability. Wetland habitat responses to climate change and the implications for restoration will be realized differently on a regional and mega-watershed level, making it important to recognize that specific restoration and management plans will require examination by habitat. Floodplains, mangroves, seagrasses, saltmarshes, arctic wetlands, peatlands, freshwater marshes and forests are very diverse habitats, with different stressors and hence different management and restoration techniques are needed. The Sundarban (Bangladesh and India), Mekong river delta (Vietnam), and southern Ontario (Canada) are examples of major wetland complexes where the effects of climate change are evolving in different ways. Thus, successful long term restoration and management of these systems will hinge on how we choose to respond to the effects of climate change. How will we choose priorities for restoration and research? Will enough water be available to rehabilitate currently damaged, water-starved wetland ecosystems? This is a policy paper originally produced at the request of the Ramsar Convention on Wetlands and incorporates opinion, interpretation and scientific-based arguments.     

Late Pleistocene and Holocene environments of the Southeastern United States from the stratigraphy and pollen content of a peat deposit on the Georgia Coastal Plain

A 5.4 m peat core from the Sandy Run Creek (SRC) valley in the upper Coastal Plain of Georgia shows that before 30 ka the valley had an aggradational sandy floor with shallow swales and low ridges of 1–2 m amplitude suggesting a braided stream pattern and a low water table. The climate from 30–25 ka was cool and dry and the vegetation open grassland with stands of pine and spruce that produced few fires. At ca. 16 ka a warmer, wetter climate caused SRC to meander and incise the valley fill removing some previously deposited sediment at the site of our peat core so that sediments of 13 ka rest directly on sediments deposited at 25 ka. After ca. 13 ka higher groundwater levels initiated vertical accretion of floodplains allowing peat accumulation in shallow depressions across the valley. Pollen from the Younger Dryas period (ca. 13–11 ka) indicates a cool, moist environment of open oak woodland, mesic trees, riparian populations of alder, and reduced levels of pine. By the early to mid Holocene, tupelo and oak dominated over pine indicating wetter conditions, while sand stringers in peat record periodic heavy rains, an elevated water table, and intervals of substantial runoff. Cooling after ca. 4.5 ka brought drier and more variable conditions. Fires increased and southern pine replaced tupelo and oak. The absence of sand stringers in peat younger than 4.5 ka indicates fewer intense rainfall events.     

New records for two mosquito predators [Turbellaria: Mesostoma] in the northeastern United States

The accumulated data indicate that mesostomid flatworms are efficient predators of mosquito larvae. This paper presents the 1st published records for 3Mesostoma Ehrenberg, including 2 predators of mosquitoes.Mesostoma californicum Hyman,Mesostoma vernale Hyman, andMesostoma sp. cf.lingua Abildgaard were discovered in a temporary pool in Massachusetts.M. californicum andM. sp. cf.lingua were found to be efficient predators on mosquitoe larvae.

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Résumé Les observations accumulées indiquent que les larves des Plathelminthes mesostomides sont des prédateurs efficaces des larves de moustiques. Cette note présente les premières mentions de 3Mesostoma Ehrenberg incluant 2 prédateurs de moustiques.Mesostoma californicum Hyman,Mesostoma vernale Hyman, etMesostoma sp. cf.lingua Abildgaard furent découverts dans une mare temporaire du Massachusetts.M. californicum etM. sp. cf.lingua ont été reconnus comme des prédateurs efficaces des larves de moustiques.

     

Caught in the Psychiatric Net: Meanings and Experiences of ADHD,Pediatric Bipolar Disorder and Mental Health Treatment Among a Diverse Group of Families in the United States

Based on a 13-month ethnographic meaning-centered study of the lived experience of behavioral and emotional disorders among children and families living in the United States, this article highlights the range of orientations to mental health problems and treatment among families from diverse backgrounds. Detailed case portraits of three families are presented to illustrate the various meanings that psychiatric diagnoses and mental health treatments take on within families, with medicalized perspectives holding less salience among African-American families relative to Euro-Americans in the study. The diverse orientations to problems among families in the study suggest that, despite the dominance of biochemical and neurological explanations for behavioral and emotional distress in contemporary U.S. culture, and especially U.S. psychiatry, nonpathological interpretations have not been wholly eclipsed by broad trends toward biologization and medicalization. Families’ experiences are positioned to reveal the allure, as well as the limitations, of biopsychiatric approaches to behavioral and emotional problems.

Biological control of invasive plants through collaboration between China and the United States of America: a perspective

For more than 100 years, classical biological control of invasive plants through screening, introducing and releasing of host-specific natural enemies from native regions has been regarded as one of the promising approaches to the management of invasive plants. Many invasive plants in the United States of America are native to China, and vice versa. China and the USA also share a number of invasive plant species, including water hyacinth (Eichhornia crassipes), alligator weed (Alternanthera philoxeroides) and cordgrass (Spartina spp.). Collaboration between the two countries on biological control benefits both the nations by reciprocal opportunities to research and exchange natural enemies, by exchanging information on common invasive species, and by providing training for students and professionals. Here we review the history of collaboration between China and the US on biological control of more than 20 invasive plants. Current collaborative projects associated with four plant species, Polygonum perfoliatum L., Trapa natans L. Pueraria montana (Lour.) Merr. var. lobata and Ailanthus altissima (Mill.) Swingle, are also covered. We prioritize 14 invasive plants as targets for future collaborative biological control based on information on their importance in introduced areas, natural enemy records, and their potential biocontrol risk to introduced ecosystems. They are: Ampelopsis brevipendunculata, Celastrus orbiculatus, Dioscorea oppositifolia, Euonymus alata, Euonymus fortunei, Ligustrum sinense, Melia azedarach, Paulownia tomentosa, Sapium sebiferum and Ulmus pumila for the US, as well as Spartina alterniflora, Ambrosia artemisiifolia, Ambrosia trifida and Solidago canadensis for China. In addition, we emphasize that we must very carefully consider any potential non-target effect when we intend to introduce and release new natural enemies. We anticipate that the high priority both countries have placed on control of invasive plants will stimulate increasing collaboration on biological control.     

Chaetognaths of the United States South Atlantic Bight: Distribution, abundance and potential interactions with newly spawned larval fish

Chaetognaths are one of the most numerous organisms in the zooplankton community off the coast of North Carolina. During two years of offshore sampling in the late winter to early spring, sixteen chaetognath species were identified, four of which had not previously been reported in the waters of the United States South Atlantic Bight. Offshore samples were dominated by Sagitta enflata Grassi, 1881, one of the larger species, which contributed > 61% of total chaetognath abundance while dominant coastal species were S. tenuis Conant, 1896 and S. hispida Conant, 1895. Abundances, body sizes and spatial distributions were determined for the most abundant chaetognath species along with the overall abundance of three common co-occurring larval fish species (spot, Atlantic croaker and Atlantic menhaden). In addition, laboratory feeding experiments were conducted using S. tenuis and S. hispida to estimate the potential impact of chaetognath predation on representative North Carolina larval fish which spawn offshore and subsequently migrate into local estuaries. Feeding rates (no. prey items day^− 1) varied with prey type and the condition (starved/fed) of the chaetognath. Weight specific daily rations (SDR) were found to vary inversely with chaetognath size, decreasing exponentially with an increase in chaetognath length. The observed abundance and distribution data indicate that wintertime chaetognath populations in offshore waters of Onslow Bay, North Carolina have the potential to interact with recently spawned larval fish and may simultaneously act as competitors, predators, or prey. Furthermore, calculations using published values of chaetognath abundance, zooplankton standing crops, and our SDR estimates indicate that chaetognaths in a representative North Carolina estuary would require a minimum of 5.96 cal m^− 3 day^− 1 to sustain their biomass. Allowing for the reported spatial and temporal variability in zooplankton abundance in these systems, chaetognaths should consume 4.4%-20.9% of the estimated total zooplankton production day^− 1. This further emphasizes the role of chaetognaths not only as predators, but also as competitors with larval fish for zooplankton food stocks in southeastern United Sates estuaries.     

Host range validation,molecular identification and release and establishment of a Chinese biotype of the Asian leaf beetle Lilioceris cheni (Coleoptera: Chrysomelidae: Criocerinae) for control of Dioscorea bulbifera L. in the southern United States

Dioscorea bulbifera, an Asian vine, is invasive in the southeastern USA. It rarely flowers but propagates from potato-like bulbils formed in leaf axils, which persist into the subsequent growing season. Lilioceris cheni Gressitt and Kimoto, a foliage-feeding beetle (Coleoptera: Chrysomelidae: Criocerinae) from Nepal, had been tested, proven to be a specialist and approved for release as a biological control agent. Regulatory delays, however, resulted in the demise of quarantine-held colonies, and acquisition of new Nepalese stock proved untenable. Searches then undertaken in southern China resulted in the collection of over 300 similar beetles. Two Chinese Lilioceris species were identified: one confirmed to be L. cheni and the other identified as Lilioceris egena (Weise). Mitochondrial analysis revealed an exact DNA match between some Chinese and one of the two Nepalese c oxidase subunit I haplotypes and all Chinese L. cheni haplotypes clustered as a single species but the comingling of the two species aroused concerns over possible hybridisation. These concerns were allayed by nuclear D2 analysis showing the absence of dual parental sequences. Nonetheless, diligence was exercised to ensure that the Chinese strains were safe to release. Abridged host testing using critical test species verified specificity. Caged releases during autumn 2011 documented the ability of adult beetles to overwinter in south Florida despite a prolonged lack of foliage. Open releases the following year produced vigorous populations that caused extensive defoliation. Preliminary observations indicate that L. cheni now contributes to the control of D. bulbifera and the bulbil-feeding L. egena should complement these effects if its host range proves appropriate.