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Multipotential nestin and Isl-1 positive mesenchymal stem cells isolated from human pancreatic islets 总被引:4,自引:0,他引:4
Eberhardt M Salmon P von Mach MA Hengstler JG Brulport M Linscheid P Seboek D Oberholzer J Barbero A Martin I Müller B Trono D Zulewski H 《Biochemical and biophysical research communications》2006,345(3):1167-1176
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In-vitro differentiation of pancreatic β-cells 总被引:13,自引:0,他引:13
B. Soria 《Differentiation; research in biological diversity》2001,68(4-5):205-219
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Pdx1 level defines pancreatic gene expression pattern and cell lineage differentiation 总被引:10,自引:0,他引:10
Wang H Maechler P Ritz-Laser B Hagenfeldt KA Ishihara H Philippe J Wollheim CB 《The Journal of biological chemistry》2001,276(27):25279-25286
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Soto C Mena R Luna J Cerbón M Larrieta E Vital P Uría E Sánchez M Recoba R Barrón H Favari L Lara A 《Life sciences》2004,75(18):2167-2180
Alloxan has been widely used to produce experimental diabetes mellitus syndrome. This compound causes necrosis of pancreatic beta-cells and, as is well known, induces oxidant free radicals which play a relevant role in the etiology and pathogenesis of both experimental and human diabetes mellitus. Previously we have reported hypoglycemic and antilipoperoxidative actions of silymarin in serum and pancreatic tissue respectively. The aim of this study was to test whether silymarin could reduce the hyperglycemia and revert the pancreatic damage in alloxan treated rats, tested with silymarin in two protocols: using both compounds simultaneously for four or eight doses, or using the compound 20 days after alloxan administration for 9 weeks. Serum glucose and insulin were determined, and pancreatic fragments were used for histology and insulin immunohistochemistry. Pancreatic islets were isolated to assess insulin and Pdx1 mRNA expression by RT-PCR. Our results showed that 72 hours after alloxan administration, serum glucose increased and serum insulin decreased significantly, whereas pancreatic tissue presented morphological abnormalities such as islet shrinkage, necrotic areas, loss of cell organization, widespread lipoid deposits throughout the exocrine tissue, and loss of beta cells, but insulin and glucagon immunoreactivity was scattered if any. In contrast the pancreatic tissue and both insulin and glucose serum levels of rats treated with silymarin were similar to those of control animals. In addition, insulin and glucagon immunoreactive cells patterns in Langerhans islets were also normal, and normal insulin and Pdx1 mRNA expression patterns were detected during pancreatic recovery in Langerhans islets. The overall results suggest that silymarin induces pancreatic function recovery demonstrated by insulin and glucagon expression protein and normoglycemia after alloxan pancreatic damage in rats. 相似文献
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Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors 总被引:57,自引:0,他引:57
The location and lineage of cells that give rise to endocrine islets during embryogenesis has not been established nor has the origin or identity of adult islet stem cells. We have employed an inducible Cre-ER(TM)-LoxP system to indelibly mark the progeny of cells expressing either Ngn3 or Pdx1 at different stages of development. The results provide direct evidence that NGN3+ cells are islet progenitors during embryogenesis and in adult mice. In addition, we find that cells expressing Pdx1 give rise to all three types of pancreatic tissue: exocrine, endocrine and duct. Furthermore, exocrine and endocrine cells are derived from Pdx1-expressing progenitors throughout embryogenesis. By contrast, the pancreatic duct arises from PDX1+ progenitors that are set aside around embryonic day 10.5 (E9.5-E11.5). These findings suggest that lineages for exocrine, endocrine islet and duct progenitors are committed at mid-gestation. 相似文献
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