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1.
Early, signal transduction-related responses in cultured tobacco cells due to methyl jasmonate (MeJa), a cell-wall-derived elicitor from Phytophthora nicotianae and chitosan, were investigated. MeJa was an effective inducer of lipid peroxidation and lipoxygenase (LOX) activity with maximum levels reached within 2 h and 4–8 h, respectively. Chitosan and the elicitor induced a transient increase (1–4 h) in lipid peroxidation. Conditioning with MeJA, followed by secondary elicitation, led to a significant increase in malondialdehyde concentration after 1 h. Chitosan and the elicitor induced transient activation of LOX with maximal values between 8 and 12 h, with preconditioning resulting in a rapid increase in LOX activity at 4 h post elicitation. MeJA did not effect phosphoprotein accumulation but conditioning led to the potentiation and differential induction of phosphoproteins due to chitosan and elicitor. The results indicate that cells are sensitized by the exposure to MeJa to respond more intensely and rapidly toward secondary elicitation by fungal pathogen derived elicitors. 相似文献
2.
Border-like cells are released by Arabidopsis ( Arabidopsis thaliana) root tips as organized layers of several cells that remain attached to each other rather than completely detached from each other, as is usually observed in border cells of many species. Unlike border cells, cell attachment between border-like cells is maintained after their release into the external environment. To investigate the role of cell wall polysaccharides in the attachment and organization of border-like cells, we have examined their release in several well-characterized mutants defective in the biosynthesis of xyloglucan, cellulose, or pectin. Our data show that among all mutants examined, only quasimodo mutants ( qua1-1 and qua2-1), which have been characterized as producing less homogalacturonan, had an altered border-like cell phenotype as compared with the wild type. Border-like cells in both lines were released as isolated cells separated from each other, with the phenotype being much more pronounced in qua1-1 than in qua2-1. Further analysis of border-like cells in the qua1-1 mutant using immunocytochemistry and a set of anti-cell wall polysaccharide antibodies showed that the loss of the wild-type phenotype was accompanied by (1) a reduction in homogalacturonan-JIM5 epitope in the cell wall of border-like cells, confirmed by Fourier transform infrared microspectrometry, and (2) the secretion of an abundant mucilage that is enriched in xylogalacturonan and arabinogalactan-protein epitopes, in which the cells are trapped in the vicinity of the root tip.Higher plants rely on their roots to acquire water and other nutrients in the soil to grow and develop ( Esau, 1977). At the tip of every growing root is a conical covering consisting of several layers of cells called the root cap that plays a major role in root protection and its interaction with the rhizosphere ( Rougier, 1981; Baluška et al., 1996; Barlow, 2003).Root tips of most plant species produce a large number of cells programmed to separate from the root cap and to be released into the external environment ( Hawes et al., 2003). This process occurs through the action of cell wall-degrading enzymes that solubilize the interconnections between root cap peripheral cells, causing the cells to separate from each other and from the root as populations of single cells ( Hawes et al., 2003). Because of their specific position at the interface between root and soil, these living cells are defined as root border cells. It has been shown that the number of these cells per root varies between plant families: from about 100 (e.g. the Solanaceae family) to several thousands (e.g. 10,000 or more for the Pinaceae; Hawes et al., 2003). It has also been suggested that species of the Brassicaceae family including Arabidopsis ( Arabidopsis thaliana) do not produce border cells ( Hawes et al., 2003). Indeed, the Arabidopsis root tip does not produce isolated border cells per se, but it does produce and release cells that remain attached to each other, forming a block of several cell layers called border-like cells ( Vicré et al., 2005; ). This also occurs in other Brassicaceae species, including rapeseed ( Brassica napus), mustard ( Brassica juncea), and Brussels sprout ( Brassica oleracea gemmifera), indicating that such an organization might be specific to this family ( Driouich et al., 2007). Open in a separate windowMorphological phenotypes of root tips showing border-like cells (BLC) of the wild type and cell wall mutants of Arabidopsis. Wild-type Columbia (Col O; A), wild-type Wassilewskija (Ws; B), mur3 (C), mur2-1 (D), kor1 (E), rsw1 (F), epc1-1 (G), arad1-1 (H), qua1-1 (I), and qua2-1 (J) are shown. Border-like cells are released from the root tip in organized cell layers (arrows) in the wild type and in all mutants examined with the exception of qua1-1 and qua2-1. Note also that border-like cell organization is similar between Columbia and Wassilewskija. M, Mucilage. Bars = 20 μm (A, B, D–H, and J) or 50 μm (C and I).The unique organization pattern of Arabidopsis border-like cells (e.g. they do not disperse individually) suggests that they might have a specific cell wall composition and/or structure that makes them resistant to cell wall-hydrolyzing enzymes or that the enzymes are not present or not functional ( Driouich et al., 2007). The only information on cell wall composition of Arabidopsis border-like cells was obtained from immunocytochemical studies, in which it has been shown that the cell wall of border-like cells is rich in pectic homogalacturonan and arabinogalactan-proteins, two wall polymers believed to be involved in cell adhesion in plants ( Vicré et al., 2005). Based on this observation, we postulated that pectic polysaccharides of the cell wall may serve as a glue to cement border-like cells together, leading to that particular organization ( Vicré et al., 2005).The cell wall of higher plants comprises mainly polysaccharides and proteoglycans. Cell wall polysaccharides are assembled into complex macromolecules, including cellulose, hemicellulose, and pectin. Cellulose forms microfibrils, which constitute an ordered, fibrous phase, whereas pectin and hemicellulose form an amorphous matrix phase surrounding the microfibrils ( Cosgrove, 1997). Pectins constitute a highly complex family of cell wall polysaccharides, including homogalacturonan, rhamnogalacturonan I, and rhamnogalacturonan II. Homogalacturonan domains consist of α- d-(1→4)-GalUA residues, which can be methyl esterified, acetylated, and/or substituted with β-(1→3)-Xyl residues to form xylogalacturonan ( Schols et al., 1995; Willats et al., 2001; Vincken et al., 2003). Deesterified blocks of homogalacturonan can be cross-linked by calcium, leading to the formation of a gel that is believed to be involved in cell adhesion ( Jarvis et al., 2003). Rhamnogalacturonan I consists of a backbone of up to 100 repeats of the disaccharide α-(1→4)-GalUA-(1→2)-rhamnose, which carries complex and variable side chains. The rhamnose residues are commonly substituted with polymeric β-(1→4)-linked d-galactosyl residues and/or α-(1→5)-linked l-arabinosyl residues ( Ridley et al., 2001). Rhamnogalacturonan II is a highly complex but conserved molecule consisting of a homogalacturonan-like backbone substituted with four different side chains containing specific sugars ( O''Neill et al., 2004).Xyloglucan is the major hemicellulosic polysaccharide of the primary wall of dicotyledonous plants, and it consists of a β- d-(1→4)-glucan backbone to which are attached side chains containing xylosyl, galactosyl-xylosyl, or fucosyl-galactosyl-xylosyl residues. Xyloglucan is the principal polysaccharide that cross-links the cellulose microfibrils. The xyloglucan-cellulose network forms a major load-bearing structure that contributes to the control of cell expansion ( Hayashi, 1989; Cosgrove, 1999).Glycoproteins, such as arabinogalactan-proteins, are also present in the cell wall matrix ( Showalter, 1993; Seifert and Roberts, 2007). Arabinogalactan-proteins are highly glycosylated members of the Hyp-rich glycoprotein superfamily. Many of these glycoproteins, the so-called classical arabinogalactan-proteins, are anchored to the plasma membrane by a glycosylphosphatidylinositol anchor and have the potential to bind both cell wall components ( Immerzeel et al., 2006) and cytosolic cortical microtubules ( Schultz et al., 2002; Sardar et al., 2006; Nguema-Ona et al., 2007). These proteoglycans have been implicated in many aspects of plant life, including cell expansion, cell signaling and communication, embryogenesis, and wound response ( Johnson et al., 2003; Seifert and Roberts, 2007; Driouich and Baskin, 2008).Although cell-to-cell interaction is a fundamental feature of plant growth and development, the molecular bases of intercellular adhesion and its loss are not fully understood ( Roberts et al., 2002; Jarvis et al., 2003; Willats et al., 2004). This study aims at investigating the role of cell wall polysaccharides in cell attachment and the organization of border-like cells in Arabidopsis. To this end, we took advantage of the recent characterization of several Arabidopsis mutants affected in the biosynthesis of different classes of cell wall polysaccharides, including pectin, xyloglucan, and cellulose. We thus examined the pattern of border-like cells released by the root tip of selected Arabidopsis mutants using microscopy and immunocytochemistry. These mutants are (1) quasimodo1-1 ( qua1-1) and qua2-1 ( Bouton et al., 2002; Mouille et al., 2007), ectopically parting cells1-1 ( epc1-1; Singh et al., 2005), and arabinan deficient1-1 ( arad 1-1; Harholt et al., 2006), which all have been reported to be possibly affected in pectin biosynthesis; (2) murus2-1 ( mur2-1) and mur3, which make altered xyloglucan ( Vanzin et al., 2002; Madson et al., 2003); and (3) radially swollen1 ( rsw1) and korrigan1 ( kor1), which are affected in cellulose biosynthesis ( Arioli et al., 1998; Nicol et al., 1998).Our data show that the organization of border-like cells had a wild-type phenotype in all of the mutants examined except in qua1-1 and qua2-1. In both of these mutants, border-like cells had lost the wild-type phenotype, as they were released as single cells separated from each other. This phenotype was far more pronounced in qua1-1 than in qua2-1. Further analysis of qua1-1 using immunocytochemistry and Fourier transform infrared microspectrometry showed a substantial loss of homogalacturonan content in border-like cells. In addition, border-like cells in the qua1-1 mutant secreted an abundant mucilage enriched in xylogalacturonan and arabinogalactan-protein epitopes. 相似文献
4.
Melatonin functions as the key growth regulator in various plant species. The mechanisms of the interactions between melatonin and cytokinins remain largely unknown. In this study, the kinetic effects of melatonin over a range of concentrations were investigated. The results showed that melatonin functioned as a positive regulator of root growth ranged from 0.1 to 100 nM. In contrast, exogenous cytokinin at 0.5–1 nM and overexpression of cytokinin biosynthesis gene ISOPENTENYLTRANSFERASE 8 (IPT8) inhibited primary root growth. Combined treatments with melatonin and cytokinin indicated that melatonin antagonized the inhibitory effect of cytokinin 6-benzylaminopurine (6-BA) on primary root elongation. Further analysis revealed that melatonin promotes primary root growth by modulating expression and distribution of auxin efflux transporters PIN2/3 and influx transporter AUX1. Moreover, the cytokinin signaling components AHK4, AHP2/3/5, and type-A ARR15 were down-regulated after melatonin treatment. The polar auxin transport inhibitor 2,3,5-triiodobenzoic acid (TIBA) impaired the promotive effect of melatonin on primary root growth, indicating that auxin transport is essential in melatonin-mediated root growth. Taken together, our data provided evidence to show that melatonin regulates primary root growth in coordination with cytokinin partially through auxin-dependent pathway. 相似文献
5.
Among the myriad of environmental stimuli that plants utilize to regulate growth and development to optimize fitness are signals obtained from various sources in the rhizosphere that give an indication of the nutrient status and volume of media available. These signals include chemical signals from other plants, nutrient signals, and thigmotropic interactions that reveal the presence of obstacles to growth. Little is known about the genetics underlying the response of plants to physical constraints present within the rhizosphere. In this study, we show that there is natural variation among Arabidopsis thaliana accessions in their growth response to physical rhizosphere constraints and competition. We mapped growth quantitative trait loci that regulate a positive response of foliar growth to short physical constraints surrounding the root. This is a highly polygenic trait and, using quantitative validation studies, we showed that natural variation in EARLY FLOWERING3 ( ELF3) controls the link between root constraint and altered shoot growth. This provides an entry point to study how root and shoot growth are integrated to respond to environmental stimuli. 相似文献
6.
Journal of Plant Growth Regulation - The regulatory role of salicylic acid (SA) has been extensively reported in plants subjected to cadmium (Cd) stress. However, the underlying mechanisms still... 相似文献
7.
The infection of lentil roots by Fusarium oxysporum Schlecht and the responses of the host cells to invading hyphae were examined by light microscopy. Hyphae from inoculum placed on the zone of cell elongation entered the roots at the juncture of epidermal cells within 8 h after inoculation. Although swollen hyphal apices were observed on the epidermal cells, root penetration occurred without formation of these structures or appressoria. The sheath of material found on the surface of uninoculated roots was absent from inoculated roots penetrated by hyphae. Prior to penetration, the epidermal cells became irregular in shape and their cytoplasm appeared to be plasmolysed or granular. Hyphae were observed in the cortex 10—12 h after inoculation and non–penetrated cortical cells were distinctly lobate. Often these lobed cells had a broad, peripheral band of diffuse cytoplasm. When hyphae were first observed in the cortical cells, the walls were ruptured and only slightly stained or unstained by toluidine blue. The inability of such walls to bind the stain may have been the result of the removal of wall components by fungal enzymes. Although extensive proliferation of hyphae was evident throughout the cortex after 24 h of incubation, the endodermis and vascular cylinder were free of hyphae for at least 72 h. Hyphae from inoculum placed on the root hairs or the root apex failed to penetrate the roots during the first 24 h of incubation. The cytological results herein are discussed in relation to the infection of field plantings by this pathogen. 相似文献
8.
The plant root is the first organ to encounter salinity stress, but the effect of salinity on root system architecture ( RSA) remains elusive. Both the reduction in main root ( MR) elongation and the redistribution of the root mass between MRs and lateral roots ( LRs) are likely to play crucial roles in water extraction efficiency and ion exclusion. To establish which RSA parameters are responsive to salt stress, we performed a detailed time course experiment in which Arabidopsis ( Arabidopsis thaliana) seedlings were grown on agar plates under different salt stress conditions. We captured RSA dynamics with quadratic growth functions ( root-fit) and summarized the salt-induced differences in RSA dynamics in three growth parameters: MR elongation, average LR elongation, and increase in number of LRs. In the ecotype Columbia-0 accession of Arabidopsis, salt stress affected MR elongation more severely than LR elongation and an increase in LRs, leading to a significantly altered RSA. By quantifying RSA dynamics of 31 different Arabidopsis accessions in control and mild salt stress conditions, different strategies for regulation of MR and LR meristems and root branching were revealed. Different RSA strategies partially correlated with natural variation in abscisic acid sensitivity and different Na +/K + ratios in shoots of seedlings grown under mild salt stress. Applying root-fit to describe the dynamics of RSA allowed us to uncover the natural diversity in root morphology and cluster it into four response types that otherwise would have been overlooked.Salt stress is known to affect plant growth and productivity as a result of its osmotic and ionic stress components. Osmotic stress imposed by salinity is thought to act in the early stages of the response, by reducing cell expansion in growing tissues and causing stomatal closure to minimize water loss. The build-up of ions in photosynthetic tissues leads to toxicity in the later stages of salinity stress and can be reduced by limiting sodium transport into the shoot tissue and compartmentalization of sodium ions into the root stele and vacuoles ( Munns and Tester, 2008). The effect of salt stress on plant development was studied in terms of ion accumulation, plant survival, and signaling ( Munns et al., 2012; Hasegawa, 2013; Pierik and Testerink, 2014). Most studies focus on traits in the aboveground tissues, because minimizing salt accumulation in leaf tissue is crucial for plant survival and its productivity. This approach has led to the discovery of many genes underlying salinity tolerance ( Munns and Tester, 2008; Munns et al., 2012; Hasegawa, 2013; Maathuis, 2014). Another way to estimate salinity stress tolerance is by studying the rate of main root ( MR) elongation of seedlings transferred to medium supplemented with high salt concentration. This is how Salt Overly Sensitive mutants were identified, being a classical example of genes involved in salt stress signaling and tolerance ( Hasegawa, 2013; Maathuis, 2014). The success of this approach is to be explained by the important role that the root plays in salinity tolerance. Roots not only provide anchorage and ensure water and nutrient uptake, but also act as a sensory system, integrating changes in nutrient availability, water content, and salinity to adjust root morphology to exploit available resources to the maximum capacity ( Galvan-Ampudia et al., 2013; Gruber et al., 2013). Understanding the significance of environmental modifications of root system architecture ( RSA) for plant productivity is one of the major challenges of modern agriculture ( de Dorlodot et al., 2007; Den Herder et al., 2010; Pierik and Testerink, 2014).The RSA of dicotyledonous plants consists of an embryonically derived MR and lateral roots ( LRs) that originate from xylem pole pericycle cells of the MR, or from LRs in the case of higher-order LRs. Root growth and branching is mainly guided through the antagonistic action of two plant hormones: auxin and cytokinins ( Petricka et al., 2012). Under environmental stress conditions, the synthesis of abscisic acid ( ABA), ethylene, and brassinosteroids is known to be induced and to modulate the growth of MRs and LRs ( Achard et al., 2006; Osmont et al., 2007; Achard and Genschik, 2009; Duan et al., 2013; Geng et al., 2013). In general, lower concentrations of salt were observed to slightly induce MR and LR elongation, whereas higher concentrations resulted in decreased growth of both MRs and LRs ( Wang et al., 2009; Zolla et al., 2010). The reduction of growth is a result of the inhibition of cell cycle progression and a reduction in root apical meristem size ( West et al., 2004). However, conflicting results were presented for the effect of salinity on lateral root density ( LRD; Wang et al., 2009; Zolla et al., 2010; Galvan-Ampudia and Testerink, 2011). Some studies suggest that mild salinity enhances LR initiation or emergence events, thereby affecting patterning, whereas other studies imply that salinity arrests LR development. The origin of those contradictory observations could be attributable to studying LR initiation and density at single time points, rather than observing the dynamics of LR development, because LR formation changes as a function of root growth rate ( De Smet et al., 2012). The dynamics of LR growth and development were characterized previously for the MR region formed before the salt stress exposure, identifying the importance of ABA in early growth arrest of postemerged LRs in response to salt stress ( Duan et al., 2013). The effect of salt on LR emergence and initiation was found to differ for MR regions formed prior and subsequent to salinity exposure ( Duan et al., 2013), consistent with LR patterning being determined at the root tip ( Moreno-Risueno et al., 2010). Yet the effect of salt stress on the reprogramming of the entire RSA on a longer timescale remains elusive.Natural variation in Arabidopsis ( Arabidopsis thaliana) is a great source for dissecting the genetic components underlying phenotypic diversity ( Trontin et al., 2011; Weigel, 2012). Genes underlying phenotypic plasticity of RSA to environmental stimuli were also found to have high allelic variation leading to differences in root development between different Arabidopsis accessions ( Rosas et al., 2013). Supposedly, genes responsible for phenotypic plasticity of the root morphology to different environmental conditions are under strong selection for adaptation to local environments. Various populations of Arabidopsis accessions were used to study natural variation in ion accumulation and salinity tolerance ( Rus et al., 2006; Jha et al., 2010; Katori et al., 2010; Roy et al., 2013). In addition, a number of studies focusing on the natural variation in RSA have been published, identifying quantitative trait loci and allelic variation for genes involved in RSA development under control conditions ( Mouchel et al., 2004; Meijón et al., 2014) and nutrient-deficient conditions ( Chevalier et al., 2003; Gujas et al., 2012; Gifford et al., 2013; Kellermeier et al., 2013; Rosas et al., 2013). Exploring natural variation not only expands the knowledge of genes and molecular mechanisms underlying biological processes, but also provides insight on how plants adapt to challenging environmental conditions ( Weigel, 2012) and whether the mechanisms are evolutionarily conserved. The early growth arrest of newly emerged LRs upon exposure to salt stress was observed to be conserved among the most commonly used Arabidopsis accessions Columbia-0 ( Col-0), Landsberg erecta, and Wassilewskija (Ws; Duan et al., 2013). By studying salt stress responses of the entire RSA and a wider natural variation in root responses to stress, one could identify new morphological traits that are under environmental selection and possibly contribute to stress tolerance.In this work, we not only identify the RSA components that are responsive to salt stress, but we also describe the natural variation in dynamics of salt-induced changes leading to redistribution of root mass and different root morphology. The growth dynamics of MRs and LRs under different salt stress conditions were described by fitting a set of quadratic growth functions ( root-fit) to individual RSA components. Studying salt-induced changes in RSA dynamics of 31 Arabidopsis accessions revealed four major strategies conserved among the accessions. Those four strategies were due to differences in salt stress sensitivity of individual RSA components (i.e. growth rates of MRs and LRs, and increases in the number of emerged LRs). This diversity in root morphology responses caused by salt stress was observed to be partially associated with differences in ABA, but not ethylene sensitivity. In addition, we observed that a number of accessions exhibiting a relatively strong inhibition of LR elongation showed a smaller increase in the Na +/K + ratio in shoot tissue after exposure to salt stress. Our results imply that different RSA strategies identified in this study reflect diverse adaptations to different soil conditions and thus might contribute to efficient water extraction and ion compartmentalization in their native environments. 相似文献
9.
Fusarium oxysporum includes nonpathogenic strains and pathogenic strains that can induce necrosis or tracheomycosis in plants. The objective of this study was to compare the abilities of a pathogenic strain (Foln3) and a nonpathogenic strain (Fo47) to colonize flax roots and to induce early physiological responses in flax cell culture suspensions. Both strains colonized the outer cortex of the root; however, plant defense reactions, i.e., the presence of wall appositions, osmiophilic material, and collapsed cells, were less frequent and less intense in a root colonized by Foln3 than by Fo47. Early physiological responses were measured in flax cell suspensions confronted with germinated microconidia of both strains. Both pathogenic (Foln3) and nonpathogenic strains (Fo47) triggered transient H 2O 2 production in the first few minutes of the interaction, but the nonpathogenic strain also induced a second burst 3 h postinoculation. Ca 2+ influx was more intense in cells inoculated with Fo47 than in cells inoculated with Foln3. Similarly, alkalinization of the extracellular medium was higher with Fo47 than with Foln3. Inoculation of the fungi into flax cell suspensions induced cell death 10 to 20 h postinoculation, with a higher percentage of dead cells observed with Fo47 than with Foln3 beginning at 14 h. This is the first report showing that early physiological responses of flax cells can be used to distinguish pathogenic and nonpathogenic strains of the soil-borne fungus F. oxysporum. 相似文献
12.
本文以沙培养法、蛭石培养法、土培养法、水培养法和MS培养基等不同的方法培养拟南芥(Arabidopsis thaliana),分析了不同培养方法对根生长发育的影响,并分别分离根的原生质体。在膜片钳记录中对不同来源的根原生质体状态进行了比较。结果表明,土培养法分离的原生质体最适于膜片钳记录。 相似文献
13.
The Arabidopsis ( Arabidopsis thaliana) genome includes eight PIN-FORMED (PIN) members that are molecularly diverged. To comparatively examine their differences in auxin-transporting activity and subcellular behaviors, we expressed seven PIN proteins specifically in Arabidopsis root hairs and analyzed their activities in terms of the degree of PIN-mediated root hair inhibition or enhancement and determined their subcellular localization. Expression of six PINs (PIN1–PIN4, PIN7, and PIN8) in root hair cells greatly inhibited root hair growth, most likely by lowering auxin levels in the root hair cell by their auxin efflux activities. The auxin efflux activity of PIN8, which had not been previously demonstrated, was further confirmed using a tobacco ( Nicotiana tabacum) cell assay system. In accordance with these results, those PINs were localized in the plasma membrane, where they likely export auxin to the apoplast and formed internal compartments in response to brefeldin A. These six PINs conferred different degrees of root hair inhibition and sensitivities to auxin or auxin transport inhibitors. Conversely, PIN5 mostly localized to internal compartments, and its expression in root hair cells rather slightly stimulated hair growth, implying that PIN5 enhanced internal auxin availability. These results suggest that different PINs behave differentially in catalyzing auxin transport depending upon their molecular activity and subcellular localization in the root hair cell.Auxin plays a critical role in plant development and growth by forming local concentration gradients. Local auxin gradients, created by the polar cell-to-cell movement of auxin, are implicated in primary axis formation, root meristem patterning, lateral organ formation, and tropic movements of shoots and roots (for recent review, see Vanneste and Friml, 2009). The cell-to-cell movement of auxin is achieved by auxin influx and efflux transporters such as AUXIN-RESISTANT1 (AUX1)/LIKE-AUX1 for influx and PIN-FORMED (PIN) and the P-glycoprotein (PGP) of ABCB (ATP-binding cassette-type transporter subfamily B) for efflux. Since diffusive efflux of the natural auxin indole-3-acetic acid (IAA; pKa = 4.75) is not favorable and PINs are localized in the plasma membrane in a polar manner, PINs act as rate-limiting factors for cellular auxin efflux and polar auxin transport through the plant body. These PINs'' properties explain why representative physiological effects of auxin transport are associated with PINs.Auxin flows from young aerial parts all the way down to the root tip columella in which an auxin maximum is formed for root stem cell maintenance and moves up toward the root differentiation zone through root epidermal cells, where a part of it travels back to the root tip via cortical cells ( Blilou et al., 2005). This directional auxin flow is supported by the polar localization of PINs: PIN1, PIN3, and PIN7 at the basal side of stele cells ( Friml et al., 2002a, 2002b; Blilou et al., 2005), PIN4 at the basal side in root stem cells ( Friml et al., 2002a), and PIN2 at the upper side of root epidermis and at the basal side of the root cortex ( Luschnig et al., 1998; Müller et al., 1998). Another interesting aspect of PIN-mediated auxin transport is the dynamics in directionality of auxin flow due to environmental stimuli-directed changes of subcellular PIN polarity, as exemplified for PIN3, whose subcellular localization changes in response to the gravity vector ( Friml et al., 2002b).An intriguing question is how different PIN proteins have different subcellular polarities, which might be attributable to PIN-specific molecular properties, cell-type-specific factors, or both. The different PIN subcellular polarities in different cell types seemingly indicate that cell-type-specific factors are involved in polarity. In the case of PIN1, however, both classes of factors appear to affect its subcellular localization because when expressed under the PIN2 promoter, PIN1 localizes to the upper or basal side of root epidermal cells, depending on the GFP insertion site of the protein ( Wiśniewska et al., 2006). A recent study demonstrated that the polar targeting of PIN proteins is modulated by phosphorylation/dephosphorylation of the central hydrophilic loop of PINs, which is mediated by PINOID (PID; a Ser/Thr protein kinase)/PP2A phosphatase ( Michniewicz et al., 2007). The central hydrophilic domain of PINs might provide the molecule-specific cue for PIN polarity, together with as yet unknown cell-specific factors. Different recycling behaviors of PINs, which show variable sensitivities to brefeldin A (BFA), also imply different molecular characters among PIN species. Most PIN1 proteins are internalized by BFA treatment, whereas considerable amounts of PIN2 remain in the plasma membrane in addition to internal accumulation after BFA treatment. Recycling and basal polar targeting of PIN1 is dependent on the BFA-sensitive guanine nucleotide exchange factor for adenosyl ribosylation factors (ARF GEFs), GNOM, which is the major target of BFA. In contrast, apical targeting and recycling of PIN2 is independent of GNOM and controlled by BFA-resistant ARF GEFs ( Geldner et al., 2003; Kleine-Vehn and Friml, 2008).In contrast to their distinct subcellular localizations, the differential auxin-transporting activities of PINs remain to be studied. The divergent primary structures of PIN proteins are not only indicative of differential subcellular polarity, but also would represent their differential catalytic activities for auxin transport. The auxin efflux activities of Arabidopsis ( Arabidopsis thaliana) PINs have been demonstrated using Arabidopsis and heterologous systems: PIN1 and PIN5 in Arabidopsis cells ( Petrásek et al., 2006; Mravec et al., 2009); PIN2, PIN3, PIN4, PIN6, and PIN7 in tobacco ( Nicotiana tabacum) Bright Yellow-2 (BY-2) cells ( Lee and Cho, 2006; Petrásek et al., 2006; Mravec et al., 2008); PIN1, PIN2, PIN5, and PIN7 in yeast ( Saccharomyces cerevisiae) cells ( Petrásek et al., 2006; Blakeslee et al., 2007; Mravec et al., 2009; Yang and Murphy, 2009); and PIN1, PIN2, and PIN7 in HeLa cells ( Petrásek et al., 2006; Blakeslee et al., 2007). Among the eight Arabidopsis PIN members, PIN1, PIN2, PIN3, PIN4, PIN6, and PIN7, which share a similar molecular structure in terms of the presence of a long central loop (hereafter called long-looped PINs; ; Supplemental Fig. S1), have been shown to catalyze auxin efflux at the cellular level. On the other hand, PIN5 and PIN8 possess a very short putative central loop (hereafter called short-looped PINs). Although PIN5 was recently shown to be localized in the endoplasmic reticulum (ER) and proposed to transport auxin metabolites into the ER lumen, its cellular function regarding its intracellular auxin-transporting activity has not been shown, and the auxin-transporting activity of PIN8 has yet to be demonstrated. In spite of the same transport directionality (auxin efflux) and similar molecular structures, the long-looped PINs exhibit sequence divergence not only in their central loop, but also in certain residues of the transmembrane domains. This structural divergence of long-looped PINs might be indicative of their differential auxin-transporting activities, which have not yet been quantitatively compared. Open in a separate windowDifferential activities of PINs in the Arabidopsis root hair. A, Two distinctive PIN groups with different central hydrophilic loop sizes. Topology of PIN proteins was predicted by four different programs as described in Supplemental Figure S1. Numbers above indicate the number of transmembrane helices for each N- and C-terminal region, and numbers below indicate the number of amino acid residues of the central hydrophilic domain. B, Representative root images of control (Cont; Columbia-0) and root-hair-specific PIN-overexpressing (PINox; ProE7: PIN-GFP or ProE7: PIN [−]) plants. Bar = 100 μm for all. C, Root hair lengths of control and PINox plants. Six to 12 independent transgenic lines (average = 8.3), and 42 to 243 roots (average = 86.8) and 336 to 2,187 root hairs (average = 727.8) per construct, were observed for the estimation of root hair length. Data represent means ± se. The root hair lengths of PIN5ox lines were significantly longer than those of the control ( P = 0.016 for PIN5ox; P < 0.0001 for PIN5-GFP1ox and PIN5-GFP2ox).To comparatively assess the cytological behaviors and molecular activities of different PIN members, it would be favorable to use a single assay system that provides a consistent cellular environment and enables quantitative estimation of PIN activity. In previous studies, we adopted the root hair single cell system to quantitatively assay auxin-transporting or regulatory activities of PINs, PGPs, AUX1, and PID ( Lee and Cho, 2006; Cho et al., 2007a). Root hair growth is proportional to internal auxin levels in the root hair cell. Therefore, auxin efflux inhibits and auxin influx enhances root hair growth ( Cho et al., 2007b; Lee and Cho, 2008). In addition, the use of a root-hair-specific promoter ( Cho and Cosgrove, 2002; Kim et al., 2006) for expression of auxin transporters enables the transporters'' biological effect to be pinpointed to only the root hair cell, thus excluding probable non-cell-autonomous effects that could be caused by the general expression of auxin transporters.In this study, we expressed five long-looped PINs (PIN1, PIN2, PIN3, PIN4, and PIN7) and two short-looped PINs (PIN5 and PIN8) in root hair cells and compared their auxin-transporting activities and cytological dynamics. To directly measure the radiolabeled auxin-transporting activities of PIN5 and PIN8, we used an additional assay system, tobacco suspension cells. Our data revealed that PINs have differential molecular activities and pharmacological responses and that the short-looped and long-looped PINs have different subcellular localizations. 相似文献
14.
本文以沙培养法、蛭石培养法、土培养法、水培养法和MS培养基等不同的方法培养拟南芥(Arabidopsis thaliana),分析了不同培养方法对根生长发育的影响,并分别分离根的原生质体.在膜片钳记录中对不同来源的根原生质体状态进行了比较.结果表明,土培养法分离的原生质体最适于膜片钳记录. 相似文献
15.
Root branching patterns in intact and decapitated flax ( Linum usitatissimumL.) roots were compared. The number of initiated primordia in the control and decapitated roots was similar, but decapitated roots produced an increased number of lateral roots owing to an increase in the number of primordia developed into the laterals. It is suggested that the apical meristem influences lateral root development only at the stage of root emergence from the parent root. 相似文献
17.
Anthropogenic CO 2 presently at 400 ppm is expected to reach 550 ppm in 2050, an increment expected to affect plant growth and productivity. Paired stomatal guard cells (GCs) are the gate-way for water, CO 2, and pathogen, while mesophyll cells (MCs) represent the bulk cell-type of green leaves mainly for photosynthesis. We used the two different cell types, i.e., GCs and MCs from canola (Brassica napus) to profile metabolomic changes upon increased CO 2 through supplementation with bicarbonate (HCO 3
-). Two metabolomics platforms enabled quantification of 268 metabolites in a time-course study to reveal short-term responses. The HCO 3
- responsive metabolomes of the cell types differed in their responsiveness. The MCs demonstrated increased amino acids, phenylpropanoids, redox metabolites, auxins and cytokinins, all of which were decreased in GCs in response to HCO 3
-. In addition, the GCs showed differential increases of primary C-metabolites, N-metabolites (e.g., purines and amino acids), and defense-responsive pathways (e.g., alkaloids, phenolics, and flavonoids) as compared to the MCs, indicating differential C/N homeostasis in the cell-types. The metabolomics results provide insights into plant responses and crop productivity under future climatic changes where elevated CO 2 conditions are to take center-stage. 相似文献
18.
Regeneration of shoots from adventitious root explants of flaxwas achieved for two of five cultivars tested. Root explantsof the cultivar Bombay regenerated buds on MS medium with varioussupplements, the best combination being 002 mg I 1 NAA,1 mg I 6-BA, 20 mg I adenine and 500 mg I cefotaxime. Adenine, in the presence of 6-BA, and cefotaxime,in the presence of both 6-BA and adenine, were found to stimulatebud initiation, but the most important factor influencing budregeneration from flax roots was genotype 相似文献
19.
The dependence of cytokinin accumulation in the shoot apexes of Arabidopsis plants on the delivery of these hormones from the roots was studied. For the estimation of cytokinin content in the cells, the immunohistochemical localization method using antibodies against zeatin riboside was used. Differential conjugation of free cytokinin bases and their ribosides was used to prevent washing of cytokinins during the dehydration process. Root cutting decreased the immunostaining of zeatin in the cells of the shoot apical meristem, thereby supporting the hypothesis about dependence of cytokinin accumulation in these cells on the hormone delivery from the roots. The level of cytokinins in the cells of the shoot apex decreased under the effect of protonophore, indicating the important role of the secondary-active transmembrane transport process of cytokinins in the maintenance of their level in the cells of the shoot apex. 相似文献
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