共查询到20条相似文献,搜索用时 31 毫秒
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Biological activity of 125iodine labelled globin mRNA in an Ehrlich ascites cell-free system 总被引:1,自引:0,他引:1
Walter Knöchel 《Molecular biology reports》1974,1(6):311-320
Globin mRNA was isolated from avian erythroblasts and labelled with 125iodine to a specific activity of 4.5×106 dpm μg-1 mRNA. The labelling procedure was modified as compared to common techniques in order to protect the mRNA against degradation. When this RNA is translated in an Ehrlich ascites cell-free system globin chains are synthesized as is demonstrated by the analysis of the in vitro synthesized products. The biological activity of 125iodine labelled mRNA has proven to be 30% of that of unlabelled mRNA. 相似文献
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Highly purified feather keratin mRNA, prepared by dissociation of mRNP particles in Na dodecyl sulphate, was translated in a wheat embryo cell-free system with similar efficiency to rabbit globin mRNA and RNA purified from cucumber mosaic virus. The only detectable products of translation of the keratin mRNA were keratin chains, which were identical to native keratin chains as judged by several different criteria. These results support previous conclusions that the keratin mRNA can be obtained in a pure state. 相似文献
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Translation of hepatic mRNA in extracts from wheat germ embryos 总被引:1,自引:0,他引:1
Extracts from wheat embryos have been used to study the incorporation of amino acids into TCA insoluble products using hepatic mRNA fractions. The properties of this system are described and compared to the incorporation obtained with poly U and rabbit globin mRNA. SDS-acrylamide gel analysis showed that the major polypeptide synthesized with globin mRNA co-migrates with rabbit globin (15 500 daltons). Rat liver products were numerous, with molecular weights from less than 10000 to greater than 65000 daltons. The KCl concentration for maximum incorporation into TCA precipitable polypeptides with hepatic mRNA was not the optimum KCl concentration for synthesis of complete products. 相似文献
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