泡桐属(Paulownia)花粉形态学的初步研究

泡桐属(Paulownia)花粉,均为近球形,具3(拟)孔沟,外壁外层有清楚的异胞网状雕纹,沟界极区明显。这些共同特征,说明泡桐属是一个种间亲缘极近的自然的属;其萌发孔类型,可作为泡桐属归入玄参科的证据之一。通过花粉形态的比较,对兰考泡桐、宜昌泡桐和建始泡桐的分类地位,提出了论证。     

泡桐属(Paulownia)花粉形态学的初步研究

泡桐属(Paulownia)花粉,均为近球形,具3(拟)孔沟,外壁外层有清楚的异胞网状雕纹,沟界极区明显。这些共同特征,说明泡桐属是一个种间亲缘极近的自然的属;其萌发孔类型,可作为泡桐属归入玄参科的证据之一。通过花粉形态的比较,对兰考泡桐、宜昌泡桐和建始泡桐的分类地位,提出了论证。     

泡桐属(Paulownia)受精作用及子实发育的研究

①泡桐属植物以虫媒传粉,在柱头室内萌发为花粉管。②从传粉至受精需时11—20天。花冠脱落后受精,受精后花柱呈现紫色。③为有胚乳种子,胚乳发育为细胞型,第一次分裂为横分裂并具有两个吸器。④胚的发育为十字花型,胚柄将胚推移到胚囊深处,当胚开始分化时,胚柄即消失。⑤种皮扩展为扁而薄的翅状,由栓化细胞构成。⑥果实为蒴果,发育期约半年,可划分为迅速增长期、缓慢增长期、充实成熟期三期。     

Detection of Mycoplasmalike Organism of Paulownia Witches′-Broom with DAPI Fluorescence Microscopy

Paulownia witches’-broom infected by mycoplasmalike organism (MLO) has been developed several cytochemical methods for diagnosis. These methods all based on the special stain reactions or abnormal fluorescence in groups of infected sieve elements as a diseased symptom,. not really on the direct detection of MLO under light microscope. This paper deals with the demonstration of MLO specific white fluorescence after DAPI staining with GMA sections of diseased young stems. Such fluorescence was absent in sections from health plants. The results were confirmed by the ulrrastrueture of MLO and the structure of sieve elements showing from PAS-TBO stained GMA sections. The described method may not only be used in accurate diagnosis of MLO diseased in different plants, but is also worth in the studies of MLO distribution in plants, MLO dynamics in plant resting stage and MLO transmission to support the theoretical basis for protection.     

Inhibition of protein tyrosine phosphatase (PTP1B) and α-glucosidase by geranylated flavonoids from Paulownia tomentosa

Protein tyrosine phosphatase 1B (PTP1B) and α-glucosidase are important targets to treat obesity and diabetes, due to their deep correlation with insulin and leptin signalling, and glucose regulation. The methanol extract of Paulownia tomentosa fruits showed potent inhibition against both enzymes. Purification of this extract led to eight geranylated flavonoids (1–8) displaying dual inhibition of PTP1B and α-glucosidase. The isolated compounds were identified as flavanones (1–5) and dihydroflavonols (6–8). Inhibitory potencies of these compounds varied accordingly, but most of the compounds were highly effective against PTP1B (IC₅₀?=?1.9–8.2?μM) than α-glucosidase (IC₅₀?=?2.2–78.9?μM). Mimulone (1) was the most effective against PTP1B with IC₅₀?=?1.9?μM, whereas 6-geranyl-3,3′,5,5′,7-pentahydroxy-4′-methoxyflavane (8) displayed potent inhibition against α-glucosidase (IC₅₀?=?2.2?μM). All inhibitors showed mixed type Ι inhibition toward PTP1B, and were noncompetitive inhibitors of α-glucosidase. This mixed type behavior against PTP1B was fully demonstrated by showing a decrease in V_max, an increase of K_m, and K_ik/K_iv ratio ranging between 2.66 and 3.69.