绵羊对化学信息的识别及其在繁殖行为中的作用

在动物生殖的各个阶段,都包含有嗅觉的作用。家畜经历了长期驯养过程之后,某些化学通讯行为有时会逐渐消失,但是它们仍然保持着野生祖先释放和感知气味的能力。 本项实验的目的在于测定绵羊产生和感知气味的能力及其在生殖中的作用。 取新疆细毛羊和哈萨克羊尿液和阴道分泌物作为外激素源,实验测定公、母羊对异性外激索的反应,记录嗅闻时间和行为表现。 结果表明,细毛公羊仅仅通过嗅觉,就有选择发情母畜的能力。它们对发情母羊化学倩息的趋近频次和嗅闻时间均高于对未发情者的频次和时间。 未曾交配过的一龄畜也能区分发情和未发情母畜的气味,但识别能力不如成畜。一定的性经验及随后的学习过程影响这种能力的发展。 实验表明,公羊在发情母畜阴遭分泌物处停留时间较长,嗅闻频次却并不高于对未发情畜阴道分泌物的次数。试畜对尿液的反应较阴道分泌物强。可见两处都含有表明性及性状况的化学信息存在。公畜对母畜化学信息表现蜷唇、舐、嗅、拱刺激源等行为。阴道分泌物所引起的行为类型则较尿液贫乏。 母羊亦表现出选择公畜化学信息的特性,它们在公羊气味处舐、排尿等。一龄畜反应亦弱于成畜。 新疆细毛羊和哈萨克公羊两个品种都表现了选择异品种信息的倾向。 绵羊是在近距离感受化学信息的,这就在一定程     

甘肃牧民安里泰试验变冬季羊羔为春羔成功

甘肃酒泉县祁明区撒里维吾尔族牧民安里泰进行延迟羊只产羔季节,改冬羔为春羔的试验已获成功,大大提高了羊只的繁殖率。安里泰改变羊只冬季产羔为春季产羔的辨法是:在头一年农历五月母羊开始发情时,把公羊和母羊分群圈养、分群放牧,避免交配。九、十月间再合群交配。这样,就把母羊的产羔期由农历的正月前后推迟到三月间。这样改变的好处是:(1)春天天气暖和,不会冻死母羊和羊羔;(2)母羊有青草吃、营养好、奶多,羊羔能吃到充足的奶和青草,使母羊和羊羔体肥健壮;(3)控制公羊、母羊的交配季节,可以使母羊能同时受孕,同时产羔,牧羊人因而就能集中精力做好接羔工作,并能改善母羊和羊羔的饲养管理。安里泰是一个养羊老手,他有丰富的养羊经验。当他改变了羊只的产羔季节后,去年他的羊羔的成活率就达95％。他的羊群发展极快,去年绵羊繁殖率达48％,山羊繁殖率达42％。     

绵羊BMP-15基因在不同产羔性状母羊中的表达量差异及分析

选择分别影响Hanna、Cambridge与Belclare三个不同品种绵羊高繁殖力的骨形态发生蛋白15(bone morphogenetic protein 15,BMP-15)为候选基因,为了解不同产羔性能母羊BMP-15基因在不同组织中的表达差异,及其与产羔数性状关联性分析,采用real time RT-PCR的方法检测BMP-15基因在蒙古羊、无角陶赛特羊、小尾寒羊3种绵羊母羊的下丘脑、垂体、卵巢、肾脏、心脏5个组织中m RNA的分布。结果表明,在具有高繁殖力的蒙古羊双羔母羊、无角陶赛特羊双羔母羊中,BMP-15基因集中表达在肾脏和卵巢,其中肾脏的表达量高于卵巢(p0.05),而其余组织表达并不明显;在小尾寒羊多羔母羊中,BMP-15基因集中表达在肾脏、心脏和卵巢,其余组织表达并不明显。在蒙古羊及无角陶赛特羊卵巢中,双羔母羊的BMP-15基因的表达量均显著高于单羔母羊(p0.05),而小尾寒羊多羔母羊的卵巢中BMP-15基因的表达量也显著高于蒙古羊的单、双羔母羊。推测BMP-15基因可能是影响以上3个品种绵羊高产的候选基因之一。     

绵羊季节性繁殖分子机制及休情季节诱导绵羊发情配种技术

季节性繁殖是限制绵羊生产效率的重要因素。季节性繁殖分子机制的深入解析是提高绵羊休情季节发情配种率的前提。研究发现,长光照与短光照条件下绵羊季节性繁殖通路中出现一系列相关信号分子变化及细胞形态学改变。基于季节性繁殖分子机制,研究者已研发出若干休情季节诱导母羊发情配种的技术或方法。本文从季节性繁殖分子机制方面总结了上述光照信号分子及垂体和下丘脑组织中细胞形态学的变化特征,及休情季节诱导母羊发情配种技术的应用效果及其利弊,并提出解决问题的关键在于寻找能够提高休情季节配种率的绿色高效的新型技术。     

山羊品种间体细胞核移植研究

萨能奶山羊是著名的奶用山羊品种,波尔山羊则是世界著名的肉用山羊品种。为了研究波尔山羊体细胞在奶山羊卵母细胞中的去分化,我们针成年波尔山羊的颗粒细胞或耳皮肤成纤维细胞作为供核细胞（试验组）,移入奶山羊中Ⅱ期的去核卵母细胞透明带下,经电融合和离子霉素与6－二甲基氨基嘌呤－DMAP）激活,直接移入同期发情奶山羊输卵管或经体内培养,将发育的重构胚移入同期发情羊子宫内。妊娠早期作B超诊断,确立妊娠的观察至足月。同时将奶山羊的35日龄胎儿成纤维细胞作供核细胞（对照组）,按试验组同样方法处理,将重构胚直接移入同期发情的奶山羊输卵管内。结果：试验组,波尔羊颗粒粒细胞与耳皮肤成纤维2细胞的融合率分别为78．2％（115／147）,57．4％（116／202）,重构胚卵裂率为85．8％（115／134）,桑椹胚,囊胚的发育率38．8％（52／134）,早期妊娠三头,分别于妊娠40,60,60日龄终止妊娠。对照组,融合率为89．5％（136／152）,早期妊娠率为42．9％（6／14）,四头受体足月分娩,产四头公羊羔,其中三头存活,一头分娩时死于肺不扩张,并体重过大,显示胎儿过大综合症。经基因型鉴定证实,这四头克隆羔羊均源于同一胎儿成纤维细胞系。以上结果表明,波尔羊体细胞核在奶山羊卵母细胞中能够去分化,并维持一定程度的发育。     

激素控制三北羊的同步发情及多胎试验

三北羊是我国名贵的羔皮羊品种。三北中的产羔率较低,一般只有105—110％,研究提高母等的产羔率,充分利用母羊的繁殖能力,在生产上有着很大的实际意义。 近年来,国际上应用合成孕激素人工控制家畜的同步发情进行了广泛的研究。如果能人工控制母畜的发情和排卵,就有可能实现一胎多羔。为了在生产上便于试验及推广,我们对三北羊进行同步发情及提高产羔率的试验,取得了初步效果,现介绍如下:     

山羊品种间体细胞核移植研究

萨能奶山羊是著名的奶用山羊品种,波尔山羊则是世界著名的肉用山羊品种.为了研究波尔山羊体细胞在奶山羊卵母细胞中的去分化,我们将成年波尔山羊的颗粒细胞或耳皮肤成纤维细胞作为供核细胞(试验组),移入奶山羊中Ⅱ期的去核卵母细胞透明带下,经电融合和离子霉素与6-二甲基氨基嘌呤(6-DMAP)激活,直接移入同期发情奶山羊输卵管或经体内培养,将发育的重构胚移人同期发情羊子宫内.妊娠早期作B超诊断,确立妊娠的观察至足月.同时将奶山羊的35日龄胎儿成纤维细胞作供核细胞(对照组),按试验组同样方法处理,将重构胚直接移入同期发情的奶山羊输卵管内.结果试验组,波尔羊颗粒粒细胞与耳皮肤成纤维细胞的融合率分别为78.2%(115/147)、57.4%(116/202),重构胚卵裂率为85.8%(115/134),桑椹胚、囊胚的发育率38.8%(52/134),早期妊娠三头,分别于妊娠40、60、60日龄终止妊娠.对照组,融合率为89.5%(136/152),早期妊娠率为42.9%(6/14),四头受体足月分娩,产四头公羊羔,其中三头存活,一头分娩时死于肺不扩张,并体重过大,显示胎儿过大综合症.经基因型鉴定证实,这四头克隆羔羊均源于同一胎儿成纤维细胞系.以上结果表明,波尔羊体细胞核在奶山羊卵母细胞中能够去分化,并维持一定程度的发育.     

影响杜泊羊冷冻胚胎移植成功率的因素

采用引进的杜泊羊冷冻胚胎,以云南当地绵羊为受体进行胚胎移植。同期发情处理了158只受体羊,同期发情率为82．91％;对102只进行了胚胎移植,实际移植率为77．86％,3个情期内移植妊娠率达74．5％;出生68只,产羔率达66．7％。分析表明,胚胎发育阶段及级别、卵巢黄体情况、以及胚胎移植技术熟练程度直接影响胚胎移植成功率;此外,受体羊的处理程序及移植后的饲养管理、移植时机的把握、移植季节以及胚胎冷冻及解冻方法也会影响杜泊羊移植妊娠率,进而影响产羔率。     

绵羊犁鼻器在繁殖中的作用

许多动物的尿液中含有重要的外激素信息,其生理意义多种多样。雌性尿液中多有表示本身性别及性状况的化学信息存在,行为学调查表明,犁鼻器的主要功能在于识别尿液气味。公羊正是依据这些化学信息识别并选择配偶的。通常公羊嗅到母羊,尤其是发情母羊尿液时,表现伸颈、抬头、卷缩上唇的特有行为型式,称之为卷唇行为或性嗅反射。这个行为是与犁鼻器的功能相联系的。它的作用在于关闭外鼻孔,堵闭会厌,使吸进的空气进入犁鼻器内,犁鼻器两侧的肌肉运动,腔内静脉窦的膨胀和收缩,又促进空气的流入和排出,如此使携带化学信息的载体不断进入犁鼻器官,成为绵羊感受化学信息的方式之一。 犁鼻器的作用可为公羊及早地、准确地选择发情的配偶提供信息;同时又能唤起公羊本身的性行为,刺激雌、雄发情及性活动的同步,对保证繁殖的成功具有一定的意义。说明绵羊犁鼻器作为化学感受器在化学通讯中特别是对繁殖行为具有重要作用。     

绵羊的卷唇行为及其生物学意义

本文研究了绵羊对不同品种公母羊的尿液、母羊粪便、阴道分泌物、身体的气味等刺激物诱导卷唇行为的发生,持续约60-120秒。实验得出:母羊尿液是诱导公羊释放卷唇行为最强的刺激物,尤以发情母羊尿液更甚,公羊、幼羊以及其它的尿液诱导卷唇行为发生的频次低。卷唇行为发生的频次有季节变化,10月最高。卷唇行为与犁鼻器的功能紧密联系,是绵羊繁殖行为的一个构成部分,通常只有公羊发生卷唇行为,但个别母羊亦有表现。     

Reproduction in Indonesian sheep and goats at two levels of nutrition

Oestrus and ovulation were recorded for eight oestrous cycles after sheep and goats were put onto maintenance or supramaintenance levels of nutrition. Oestrous cycles were longer and more variable in goats than in sheep (20.3 ± 0.3 and 17.4 ± 0.2 days respectively), but in neither species was the incidence of oestrus affected by level of nutrition. There were no significant differences between sheep and goats in average body weight or mean ovulation rate. Animals fed at a maintenance level showed no significant changes in average body weight (19.0 ± 0.3 kg) or mean ovulation rate (1.69 ± 0.01). At the supramaintenance level, on the other hand, mean body weight increased progressively to a maximum of 24.8 ± 0.5 kg, and mean ovulation rate increased to a maximum of 2.65 at the sixth oestrus after the beginning of supramaintenance feeding. The effect of supramaintenance feeding on ovulation rate was reflected in subsequent lamb and kid production.     

Secretion of anti-parasite substances and leukotrienes from ovine gastrointestinal tissues and isolated mucosal mast cells

The presence of larval migration inhibitory (LMI) compounds in the gastrointestinal mucus of nematode resistant sheep has been shown previously to be associated with increased numbers of gastrointestinal mucosal mast cells (MMC) and globule leukocytes (GL). This experiment was designed to determine if LMI compounds were secreted by MMC/GL in response to nematode antigenic challenge and if so, could secretion account for levels observed in mucus. Rommey sheep were immunized by repeated cycles of infection with Trichostrongylus colubriformis or Haemonchus contortus larvae and anthelmintic treatment. After slaughter, gastrointestinal tissue was taken for examination of histology and mucus anti-parasite activity. Segments of small intestine were ligatured to form sacs which were incubated with exsheathed nematode larvae or larval excretory/secretory antigens. Tissue slices from small intestine or abomasum were also incubated with nematode larvae or antigens. After homologous challenge, levels of leukotrienes secreted into small intestinal tissue sacs were significantly higher than levels in heterologously challenged sacs or unimmunized sheep intestinal sacs challenged with larvae of any nematode species (279.4±33.7, 141.0±27.8 and 39.5±15.2 ng h⁻¹ respectively). Tissue slices gave a similar pattern of leukotriene secretion. LMI activity was also significantly elevated in intestinal sacs from immunized sheep challenged homologously with nematode larvae or antigen (64±10 and 68±14% respectively cf. heterologous challenge 32±10% and unimmunized sheep sacs 15±6%). Histological examination of abomasal and small intestinal sections showed that immunized sheep had significantly greater numbers of MMC/GL than unimmunized sheep. MMC/GL isolated and purified from immunized sheep secreted leukotrienes and compounds having LMI activity when cultured with homologous nematode larvae or antigens. Secretion of leukotrienes and molecules having LMI activity from MMC/GL could account for the levels of these substances observed in small intestinal mucus.     

Effects of chronic fluorosis on electrocardiogram in sheep

This study was carried out to evaluate the effects of chronic fluorosis by means of the electrocardiograms in sheep. Ten sheep with fluorosis living around a volcanic mountain (Tendürek Mount) in East Anatolia in Turkey and 10 healthy sheep were used. Leads I, II, III, aVR, aVL, aVF, V₂, V₄, and V₁₀ were recorded in the electrocardiographs of the sheep. All waves were seen in all derivations. The P-Q interval was significantly (p<0.05) prolonged and sinus bradycardia was observed in the sheep with fluorosis. As a result of this, the number of heart beats was decreased significantly (p<0.05); that is, the number of heart beats was 110±15 in the control group and 75±10 in sheep with fluorosis.     

Analysis of Geographic and Pairwise Genetic Distances Among Sheep Populations

This study assessed the usefulness of geographic and pairwise genetic distances in the characterization of five sheep populations using 15 microsatellite markers. The average F statistics across loci were F _IT = 0.523 ± 0.140, F _ST = 0.363 ± 0.131, and F _IS = 0.263 ± 0.092. The average heterozygosity was 0.716 ± 0.069, polymorphism information content was 0.691 ± 0.070, and effective number of alleles was 3.736 ± 0.998. Sheep populations clustered into group 1 (Hu and Tong breeds) and group 2 (small-tailed Han, Wadi, and Tan breeds). Reynolds’ distance varied from 0.0062 to 0.0499, and the range of gene flow (N _m) was 4.8834–40.0726 among the sheep populations. The results showed that the genetic structure of the five populations was not consistent with their genetic distances, and the population genetic divergence was not linearly related to geographic distance as indicated by a Mantel test (P = 0.7936).     

Conservation genetics in Chinese sheep: diversity of fourteen indigenous sheep (Ovis aries) using microsatellite markers

The domestic sheep (Ovis aries) has been an economically and culturally important farm animal species since its domestication around the world. A wide array of sheep breeds with abundant phenotypic diversity exists including domestication and selection as well as the indigenous breeds may harbor specific features as a result of adaptation to their environment. The objective of this study was to investigate the population structure of indigenous sheep in a large geographic location of the Chinese mainland. Six microsatellites were genotyped for 611 individuals from 14 populations. The mean number of alleles (±SD) ranged from 7.00 ± 3.69 in Gangba sheep to 10.50 ± 4.23 in Tibetan sheep. The observed heterozygote frequency (±SD) within a population ranged from 0.58 ± 0.03 in Gangba sheep to 0.71 ± 0.03 in Zazakh sheep and Minxian black fur sheep. In addition, there was a low pairwise difference among the Minxian black fur sheep, Mongolian sheep, Gansu alpine merino, and Lanzhou fat‐tailed sheep. Bayesian analysis with the program STRUCTURE showed support for 3 clusters, revealing a vague genetic clustering pattern with geographic location. The results of the current study inferred high genetic diversity within these native sheep in the Chinese mainland.     

Animal Board Invited Review: Meta-analysis of genetic parameters for resilience and efficiency traits in goats and sheep

Genetic selection focused purely on production traits has proven very successful in improving the productive performance of livestock. However, heightened environmental and infectious disease challenges have raised the need to also improve the resilience of animals to such external stressors, as well as their efficiency in utilising available resources. A better understanding of the relationship between efficiency and production and health traits is needed to properly account for it in breeding programmes and to produce animals that can maintain high production performance in a range of environmental conditions with minimal environmental footprint. The aim of this study was to perform a meta-analysis of genetic parameters for production, efficiency and health traits in sheep and goats. The dataset comprised 963 estimates of heritability and 572 genetic correlations collated from 162 published studies. A threelevel meta-analysis model was fitted. Pooled heritability estimates for milk production traits ranged between 0.27 ± 0.03 and 0.48 ± 0.13 in dairy goats and between 0.21 ± 0.06 and 0.33 ± 0.07 in dairy sheep. In meat sheep, the heritability of efficiency traits ranged from 0.09 ± 0.02 (prolificacy) up to 0.32 ± 0.14 (residual feed intake). For health traits, pooled heritability was 0.07 ± 0.01 (faecal egg count) and 0.21 ± 0.01 (somatic cell score) in dairy goats and 0.14 ± 0.04 (faecal egg count) and 0.13 ± 0.02 (somatic cell score) in dairy sheep. In meat sheep, the heritability of disease resistance and survival traits ranged between 0.07 ± 0.02 (mastitis) and 0.50 ± 0.10 (breech strike). Pooled estimates of genetic correlations between resilience and efficiency traits in dairy goats were not significantly different from zero with the exception of somatic cell score and fat content (?0.19 ± 0.01). In dairy sheep, only the unfavourable genetic correlation between somatic cell score and protein content (0.12 ± 0.03) was statistically significant. In meat sheep only, the correlations between growth and faecal egg count (?0.28 ± 0.11) as well as between growth and dagginess (?0.33 ± 0.13) were statistically significant and favourable. Results of this meta-analysis provide evidence of genetic antagonism between production and health in dairy sheep and goats. This was not observed in meat sheep where most of the pooled estimates had high standard errors and were non-significant. Based on the obtained results, it seems feasible to simultaneously improve efficiency and health in addition to production by including the different types of traits in the breeding goal. However, a better understanding of potential trade-offs between these traits would be beneficial. Particularly, more studies focused on reproduction and resilience traits linked to the animal’s multi-trait response to challenges are required.     

Superovulation and recovery of zygotes suitable for microinjection in different breeds of sheep

This study investigated the appropriateness of different superovulatory protocols in various breeds of sheep for obtaining a maximum of zygotes suitable for microinjection. Animals were mated either once or two to three times to fertile rams. In Experiment 1, a 24 h interval between a two to three times mating and egg recovery resulted in 42.2% suitable zygotes whereas with single mating only 10.4% fertilized eggs were obtained. The extension of the interval to 40 h associated with a two to three times mating resulted in a recovery of 42.9% fertilized eggs but most (70%) of these were already at the two-cell stage. In Experiment 2, eCG resulted in similar superovulatory responses in Merino ewes as the more labour requiring FSH treatment (8.1 ± 4.5 versus 7.5 ± 4.1 corpora lutea (CL); 6.3 ± 3.0 versus 6.8 ± 4.0 oocytes/ zygotes; 39.4% versus 40.6% fertilization rate). In Experiment 3, following superovulation with pFSH (Folltropin®) the number of CL was not different among Merino, Finn, Crossbreds (Blackface X Finn) and Texel sheep (8.6 ± 5.2; 10.3 ± 4.5; 8.5 ± 3.8; 8.2 ± 2.8, respectively) as was the number of recovered oocytes/ zygotes (7.4 ± 5.6; 9.8 ± 4.3; 7.3 ± 3.8; 6.4 ± 2.9, respectively). However, the number of unfertilized ova was higher (P < 0.05) in Finn sheep as compared with Crossbreds and Texel sheep (5.0 ± 3.3 versus 2.2 ± 2.3 and 1.9 ± 2.6). Similarly, the fertilization rate was higher (P < 0.05) in Crossbreds and Texel sheep (64.4% and 65.5%) as compared with Finn and Merino sheep (38.3% and 42.5%). In Experiment 4, it was shown that in Merino sheep purified FSH supplemented with 68.6% LH resulted in lower (P < 0.05) superovulatory responses as compared with purified FSH supplemented with 133.1% LH or Folltropin (LH contamination 0.1%) (4.7 ± 3.3 versus 8.8 ± 3.8 and 8.6 ± 5.2 CL; 3.8 ± 2.5 versus 7.4 ± 3.6 and 7.4 ± 5.6 oocytes/zygotes, respectively). A three times repeated superovulatory treatment and oviductal flush per animal at monthly intervals did reduce (P < 0.05) the number of CL, but had no deleterious effect on zygote yields and the percentage of microinjectable zygotes. We conclude that (1) at least a two to three times mating is required to obtain acceptable fertilization rates; (2) the interval between mating and recovery should be 24–26 h in order to obtain zygotes; (3) eCG results in similar superovulatory responses as FSH; (4) Folltropin® is a suitable drug to induce superovulation in sheep; (5) the LH content of the FSH preparation plays a significant role in the superovulatory response of sheep; (6) superovulation and embryo recoveries can be repeated at least three times per animal without decrease in efficiency.     

Improvements in the conception rate,milk composition and embryo quality of rabbit does after dietary enrichment with n-3 polyunsaturated fatty acids

This work attempts to confirm the effect of an enriched diet with n-3 polyunsaturated fatty acids (PUFA) trying to mitigate the reproductive performances issues such as low conception rate of primiparous rabbits. A total of 127 does were fed ad libitum throughout their two first cycles with two diets with different fat sources: mixed fat in the control and salmon oil in the enriched one, with 3.19 g/100 g (n=63 does) and 28.77 g/100 g (n=64 does) of n-3 of the total fatty acid, respectively. Feed intake was similar between groups (P>0.05). Plasma progesterone concentration was higher in the enriched females than in control ones at 7 (30.9±2.18 v. 23.9±2.30 ng/ml, respectively; P=0.029) and 14 (38.7±2.18 v. 28.2±2.30 ng/ml, respectively; P=0.001) days of first gestation. Considering both cycles, reproductive parameters of mothers (fertility, duration of gestation and prolificacy) and litter parameters (weight at parturition and weaning, mortality and average daily gain (ADG) of kits during lactation) were similar in both groups. However, individual measurements of neonates of enriched group improved 5.87%, 7.10% and 18.01% (P<0.05) in terms of crown-rump length, biparietal and thoracic diameters, respectively, compared to control ones at first parturition. It is noteworthy that at the second insemination, critical point in rabbit, fertility rate of enriched group did not decline as sharply as in the control group (89.7% v. 76.6%, respectively; P=0.067), although ADG and littler weight were slightly lower at the second lactation after PUFA enrichment (P<0.05). Total PUFA and unsaturated index of milk of enriched does group were significantly elevated than in control one (33.3±0.02 v. 23.2±0.02 g/100 g and 1.20±0.00 v. 0.86±0.00, respectively; P<0.05). Finally, plasma progesterone, ovulation rate, fertility and embryo development at 3.5 days after the artificial insemination were similar between diets (P>0.05), but embryo apoptosis rate was higher in control group than in enriched one (31.1±4.56% v. 17.1±3.87%, respectively; P<0.05). In conclusion, dietary PUFA enrichment from the rearing and throughout two productive cycles improved plasma progesterone during pregnancy, fertility, milk fatty acid profile and neonates development of primiparous supporting the beneficial effect of n-3 PUFA supplementation in rabbit does.     

吉西他滨热化疗治疗肝癌的临床效果及对血清MGMT水平的影响

目的:探讨吉西他滨热化疗治疗肝癌的临床效果及对血清O6-甲基鸟嘌呤DNA甲基转移酶(O6-methylguanine-DNA methyltransferase,MGMT)水平的影响。方法:选取2014年2月-2017年4月在我院诊治的59例肝癌患者,根据治疗方法的方法不同分为观察组30例、对照组29例,对照组给予常规化疗,化疗观察3个周期;观察组在对照组化疗的基础上给予吉西他滨热灌注化疗,比较两组的临床疗效、不良反应的发生情况、生存情况及治疗前后血清MGMT表达的变化情况。结果:观察组患者的热灌注化疗均成功进行,治疗前后患者的体温、呼吸频率、心率、血压等对比无显著差异(P0.05)。治疗后,观察组总有效率是66.7%,显著高于对照组(31.0%)(P0.05)。两组治疗期间的毒性反应发生率间无显著差异(P0.05)。两组治疗后血清MGMT表达都较治疗前显著降低(P0.05),且观察组的血清MGMT表达明显比对照组低(P0.05)。随访至2017年11月30日,观察组的疾病进展时间(Time to Progression,TTP)、生存时间(overall survival,OS)分别为8.11±2.19个月和14.29±1.87个月,都显著高于对照组的6.22±1.82个月和11.48±2.19个月(P0.05)。结论:吉西他滨热化疗治疗肝癌可提高疗效,延长患者的生存时间,且不增加毒副反应,可能与其有效降低患者血清MGMT表达有关。