食用真菌发酵液SOD检测

目的检测食用真菌培养液中是否含有SOD（超氧化物歧化酶）,及经长期保存后的SOD酶活性。方法选取3株实验室保存的食用真菌,经液体培养后,长期保存于液体培养基中2年,用邻苯三酚法检测各培养液的SOD酶活性。结果3种培养液中SOD酶活性均为阳性。结论SOD存在于食用菌培养液中,且经长期保存仍有很强的SOD酶活性。     

不同发酵茶中优势真菌的抗氧化酶活性

目的在云南普洱茶、广州小青柑、广西六堡茶、湖南熙茯茶及陕西泾阳茯砖茶分离到9种真菌的基础上,探讨9种真菌发酵液和发酵茶沸水冲泡液的抗氧化酶活性。方法采用抗氧化试剂盒测定真菌发酵液及发酵茶沸水冲泡液的抗氧化酶活性;利用Past 3软件对9种真菌发酵液抗氧化酶活性和5种发酵茶沸水冲泡液的抗氧化酶活性进行主成分分析。结果通过比较不同真菌发酵液的抗氧化酶活性表明,小青柑中分离到阿曲霉Q1的CAT(过氧化氢酶)活性最高,酶活力单位为(4.37±0.15)U/mL,熙茯茶中分离到谢瓦曲霉X1的POD(过氧化物酶)活性最高,酶活力单位为(21.67±1.05)U/mL,其次为泾茯茶中分离到赤散囊菌J17,酶活力单位为(18.59±2.74)U/mL;六堡茶中分离到的黑曲霉L1的T-SOD(总超氧化物歧化酶)的活性最高,酶活力单位为(71.11±3.90)U/mL,其次为普洱茶中分离到篮状菌P1,酶活力单位为(59.29±1.42)U/mL;通过比较不同发酵茶沸水冲泡液的抗氧化酶活性表明,5种发酵茶的CAT(F=37.409,P0.01),POD(F=164.268,P0.01),T-SOD(F=26.639,P0.01)活性差异均有统计学意义。其中,泾茯茶冲泡液的CAT活性最高,而其余4种茶叶冲泡液均无CAT活性;普洱茶与泾茯茶冲泡液的POD活性最高,酶活力单位分别为(18.11±0.71)和(18.11±0.64)U/mL;普洱茶和六堡茶冲泡液的T-SOD活性最高,酶活力单位分别为(48.72±0.43)和(45.82±2.02)U/mL;通过对9种真菌发酵液抗氧化酶活性的PCA分析表明,谢瓦曲霉X1、赤散囊菌J17、阿曲霉Q1、烟曲霉Q2、绳状篮状菌Q3和冠突曲霉L2的抗氧化酶活性相近,篮状菌P1、篮状菌P2和黑曲霉L1的抗氧化酶活性相近;通过对5种发酵茶沸水冲泡液抗氧化酶活性的PCA分析表明,小青柑与熙茯茶沸水冲泡后的抗氧化酶活性相近,六堡茶、普洱茶和泾茯茶沸水冲泡后的抗氧化酶活性相近。结论不同发酵茶的优势真菌不同,其真菌发酵产物的抗氧化酶活性不同,且不同发酵茶冲泡液的抗氧化酶活性也不同。     

黑斑蛙精巢MDA和抗氧化酶对铅、镉暴露的生态毒性响应

以健康性成熟黑斑蛙为供试动物,以精巢组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性为指标,进行了水体铅、镉暴露的生态毒性响应研究.结果表明:(1)精巢MDA含量随铅、镉暴露浓度的升高而明显增加,且呈明显的浓度-效应关系.说明低水平铅、镉的长期暴露对黑斑蛙精巢具有一定的损伤作用;(2)SOD活性在各处理组响应变化不明显,CAT、GSH-Px活性则被显著诱导,说明GSH-Px、CAT在铅、镉引起的精巢抗氧化损伤中起着重要作用;(3)3种抗氧化酶相比,GSH-Px活性对铅、镉暴露响应最敏感,SOD活性的响应最不明显,精巢GSH-Px活性是指示铅、镉暴露的优选生物标志物。     

多炔化合物氧化胁迫下稗草细胞保护酶系的反应

用多炔类化合物1-苯基-4-(3,4-亚甲二氧)-苯基丁二炔(简称化合物5)处理稗草(Echinochloa crusgalli)愈伤组织,经紫外光(320～400nm)照射后,诱导细胞内形成氧化胁迫环境。利用生化酶学方法,测定几种保护酶系在氧化环境下的活性变化。发现经化合物5和照光处理后,可诱导激活细胞内的谷胱甘肽-S-转移酶(GST)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化物酶(POD)的活性,而超氧化歧化酶(SOD)则表现为活性受抑制。以0．1～10mg／L的浓度处理,所测GST、GSH-Px和POD的照光诱导活性明显高于未经照光处理的活性。其中以10mg／L,的浓度处理,照光所提高3种酶活性的百分率分别为10．47％、113．68％和166．68％。以1mg／L和10mg／L的浓度处理,照光对SOD的抑制百分率分别为50．25％和76．46％。测定结果表明：在外源光敏物质引起细胞内的氧化胁迫环境下,可激活细胞内保护酶的活性,用于抵御氧化逆境对细胞的损伤。而SOD则可能是化合物5光活化抑制稗草生长的生化作用靶标酶之一。     

斜纹夜蛾幼虫感染莱氏野村菌后的抗氧化酶活性变化

【目的】研究斜纹夜蛾Spodoptera litura幼虫感染莱氏野村菌Nomuraea rileyi后的抗氧化防御机制。【方法】通过测定斜纹夜蛾各龄幼虫超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)等的活性,探讨染菌后不同侵染阶段,幼虫抗氧化酶活性的变化趋势和不同接种方法对抗氧化酶活性的影响。【结果】在斜纹夜蛾染菌幼虫和未接种幼虫(对照)中均未测出POD活性;各龄幼虫在接种莱氏野村菌后SOD和CAT活性显著高于对照,但随着侵染过程的进行,2-3龄幼虫的SOD和CAT活性在16 h左右达峰值后迅速降低,而4-5龄幼虫SOD和CAT活性自染菌初期增强后,变化较平缓,至60-72 h后才低于对照。喷雾法与浸蘸法接种后,各龄幼虫SOD和CAT活性显著高于对照,且对于2-3龄幼虫,浸蘸法对抗氧化酶活性的影响显著高于喷雾法,而对于4-5龄幼虫而言两处理方式之间活性差异不显著。【结论】斜纹夜蛾感染莱氏野村菌后,其体内抗氧化酶活性变化很大,SOD和CAT活性先升后降,且其变化趋势与幼虫所处的发育阶段密切相关。在体壁接种量相当的情况下,浸蘸法对2-3龄幼虫SOD和CAT活性的影响大于4-5龄幼虫。     

红葡萄酒对大鼠肝脏抗氧化酶和脂质过氧化的影响

选用雄性SD大鼠,分别灌胃红葡萄酒、酒精及水。实验90 d中每隔30 d处死一批动物,测定大鼠肝脏匀浆组织中的超氧化物歧化酶(Superoxide dismutase SOD)、过氧化氢酶(Catalase CAT)、谷胱甘肽过氧化物酶(Glutathione peroxidase GSH-Px)活性和脂质过氧化产物丙二醛(Malondialdehyde MDA)含量变化。观察摄入红葡萄酒后大鼠肝脏抗氧化酶活性变化及对肝脏脂质过氧化的影响。结果表明,红葡萄酒能提高SOD活性,且SOD活性与灌胃时间、剂量有一定关系;长期红葡萄酒和酒精摄入可诱导CAT活性增强,加剧肝脏的脂质过氧化(LPO)作用;红葡萄酒组、酒精组0.63、1.25 g/kg剂量GSH-Px活性均明显升高(P<0.05),酒精组1.88 g/kg剂量有极显著性差异(P<0.01);试验初期,红葡萄酒大剂量显著降低肝脏中MDA的含量。试验中期,红葡萄酒中大剂量显著降低MDA含量。试验末期,红葡萄酒大剂量和酒精中大剂量显著升高肝脏中MDA含量。     

不同季节荷斯坦牛3个微卫星座位与GSH-Px、SOD和Na+/K+-ATP酶活性及日产奶量的关系

选择分别与谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和Na⁺/K⁺-ATP酶基因紧密连锁的3个微卫星座位BMS2258、SOD1和BM723, 采用PCR及非变性聚丙烯酰胺凝胶电泳分析其在130头荷斯坦牛中的遗传变异, 计算了3个微卫星座位的多态信息含量、有效等位基因数和遗传杂合度, 并利用最小二乘法拟合线性模型初步探索了它们与荷斯坦牛夏、秋季GSH-Px、SOD、Na⁺/K⁺-ATP酶活性及日产奶量的关系。结果表明, 3个微卫星座位与其紧密连锁基因的酶活性及日产奶量均存在显著相关(P<0.05)。BMS2258座位182 bp/164 bp的GSH-Px活性和日产奶量对应的最小二乘均值较高; SOD1座位148 bp/146 bp对应的SOD活性的最小二乘均值较高, 148 bp/148 bp对应的日产奶量最小二乘均值较高; BMS2258座位161 bp/111 bp对应的Na⁺/K⁺-ATP酶活性和日产奶量的最小二乘均值较高, 它们是各自座位上的最有利基因型。     

迷迭香对运动大鼠肝脏组织脂质过氧化损伤保护作用的研究

本实验选用SD(Sprague Dawley)大鼠,建立大强度耐力训练模型,研究迷迭香对运动大鼠肝脏组织脂质过氧化损伤保护作用。结果显示,1)迷迭香可降低血清丙氨酸氨基转移酶活性,升高肝组织丙氨酸氨基转移酶的活性,都有显著性差异(P<0.05);2)迷迭香可以不同程度地增强肝脏组织中抗氧化酶SOD(superoxide dis-mutase)、CAT(catalase)和GSH-Px(glutathione peroxidase)的活性,其中SOD和CAT的活性增加在安静和运动状态下都有显著性差异(P<0.05),GSH-Px的活性增加在运动状态下具有显著性差异(P<0.05);3)迷迭香可以降低肝脏组织中MDA(malondialdehyde)的含量,无显著性差异(P>0.05)。结论:迷迭香可以增加肝脏组织中的抗氧化酶活性,减轻大强度耐力训练对大鼠肝脏组织造成的脂质过氧化损伤。并且在同一状态下对不同的抗氧化酶活性影响不同。     

异源副粘病毒感染对雏鸭血清抗氧化酶活性的影响

以雏鸭为试验对象,研究不同来源的副粘病毒感染对雏鸭血清中抗氧化酶活性的影响。选择20日龄健康雏鸭100只,随机分成Ⅰ、Ⅱ、Ⅲ、Ⅳ组,Ⅰ组为对照组,皮下注射0.5 mL/只生理盐水,Ⅱ、Ⅲ、Ⅳ组分别皮下注射0.5 mL/只稀释的鸡源、鹅源及鸭源副粘病毒SPF胚液。分别在攻毒后7、14、21、28 d时,随机抽取Ⅰ、Ⅱ、Ⅲ、Ⅳ组雏鸭各5只进行血液采集与血清分离,测定雏鸭血清中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)的活性与含量,研究异源副粘病毒感染对雏鸭血清中抗氧化酶活性的影响,以及脂质过氧化程度与抗氧化酶活性的变化趋势。结果表明,在攻毒后不同时间内,感染异源副粘病毒的雏鸭血清中的SOD、CAT、GSH-Px活性和MDA活性与含量变化显著不同,揭示异源副粘病毒对雏鸭血清自由基代谢产生了不同程度影响,脂质过氧化程度与SOD、GSH-Px活性密切相关。     

镉对长江华溪蟹酶活性及脂质过氧化的影响

本实验于2005年3-7月,采用急性毒性实验方法,研究了镉(Cd)对长江华溪蟹(Sinopotamon yangtsekiense)酶活性及脂质过氧化产物的影响.Cd处理设5个浓度组,分别为7.25mg/L、14.5mg/L、29mg/L、58mg/L和116mg/L,实验同时设对照组.分别在24h、48h、72h和96h取肝胰腺和鳃进行超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性的测定和脂质过氧化产物丙二醛(MDA)含量的测定.结果显示,在实验剂量范围内,随着镉浓度的增加和处理时间的延长,SOD和GSH-Px的活性均呈现先增高后降低的趋势,并存在组织差异性;而组织中MDA的含量随镉浓度的增加和处理时间的延长而升高.当处理时间为48h、Cd浓度为14.5mg/L时,肝胰腺和鳃中SOD活性均达最大值,较对照组差异极显著;当处理时间为48h、Cd浓度为7.25mg/L时,肝胰腺中GSH-Px活性达最大值,较对照组差异极显著;当处理时间为24h、Cd浓度为14.5mg/L时,鳃中GSH-Px活性达最大值,较对照组差异极显著.伴随处理时间和Cd浓度的增加,SOD和GSH-Px的活性开始逐渐下降.肝胰腺和鳃中GSH-Px/SOD的比值在对照组中处于平衡状态,但是在处理组中失去了平衡,有降低的趋势.结论:SOD与GSH-Px的活性和MDA含量的变化可以灵敏地反映Cd的胁迫程度及毒性大小.     

The Level of Secreted Laccase Activity in the Edible Fungi and their Growing Cycles are Closely Related

This article focuses on the relation between laccase-secreting ability and growing cycle in the edible fungi. First, laccase activities of fifteen different edible fungi were detected and determined by plate assay and spectrophotometric method using 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) as the substrate. The results showed the laccase-secreting ability in the edible fungi and their growing cycles are closely related. The edible fungi strains with short growing cycles originate from their high levels of secreted laccase activity. However, those strains require long growing cycles due to the low levels of secreted laccase, even no detectable laccase activity. The research provides the first evidence on the corresponding relation between the level of secreted laccase activity and growth cycles of edible fungi. Our study has significantly increased the understanding of the role of laccase in the growth and development of edible fungi.     

Temporal and regional changes of superoxide dismutase and glutathione peroxidase activities in rats exposed to focal cerebral ischemia

Reactive oxygen species are important cause of tissue injury during cerebral ischemia and reperfusion (I/R). Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) are intracellular enzymes responsible for endogenous antioxidant defense of tissues affected by I/R. The aim of this study was to examine temporal and regional changes of SOD and GSH-Px activities in animals exposed to transient focal cerebral ischemia. Male Wistar Hannover rats were subjected to the right middle cerebral artery occlusion for 2?h. The animals were sacrificed immediately, 0·5, 1, 2, 3, 6, 24, 48, 72 or 168?h after ischemic procedure. SOD and GSH-Px activities were determined spectrophotometrically in the hippocampus and parietal cortex, both unilaterally and contralaterally to the occlusion. Sham-operated animals were used as the control group. Our results indicated that transient focal cerebral ischemia causes significant changes in SOD activities in the hippocampus and parietal cortex such as in GSH-Px activities in the parietal cortex, unilaterally and contralaterally to the lesion in rats during different reperfusion periods. Statistically significant activation of GSH-Px was registered neither in the right nor in the left hippocampus of ischemic animals. Copyright ? 2012 John Wiley & Sons, Ltd.     

Insulin, catecholamines, glucose and antioxidant enzymes in oxidative damage during different loads in healthy humans

Exercise, insulin-induced hypoglycemia and oral glucose loads (50 g and 100 g) were used to compare the production of malondialdehyde and the activity of antioxidant enzymes in healthy subjects. Twenty male volunteers participated in the study. Exercise consisted of three consecutive work loads on a bicycle ergometer of graded intensity (1.5, 2.0, and 2.5 W/kg, 6 min each). Hypoglycemia was induced by insulin (Actrapid MC Novo, 0.1 IU/kg, i.v.). Oral administration of 50 g and 100 g of glucose was given to elevate plasma glucose. The activity of superoxide dismutase (SOD) was determined in red blood cells, whereas glutathione peroxidase (GSH-Px) activity was measured in whole blood. The concentration of malondialdehyde (MDA) was determined by HPLC, catecholamines were assessed radioenzymatically and glucose was measured by the glucose-oxidase method. Exercise increased MDA concentrations, GSH-Px and SOD activities as well as plasma noradrenaline and adrenaline levels. Insulin hypoglycemia increased plasma adrenaline levels, but the concentrations of MDA and the activities of GSH-Px and SOD were decreased. Hyperglycemia increased plasma MDA concentrations, but the activities of GSH-Px and SOD were significantly higher after a larger dose of glucose only. Plasma catecholamines were unchanged. These results indicate that the transient increase of plasma catecholamine and insulin concentrations did not induce oxidative damage, while glucose already in the low dose was an important triggering factor for oxidative stress.     

The relation between selenium, zinc and copper concentration and the trace element dependent antioxidative status.

An imbalance in the antioxidative system was connected with the development of a number of pathological processes. In order to receive values of a healthy group and to evaluate pathological changes of the trace element dependent antioxidative status in future, we investigated 99 healthy volunteers (45 male and 54 female, mean age 37.4 +/- 11.7 years). We determined the concentrations of Se, Cu and Zn, the concentrations of malondialdehyde (MDA) and the activities of the Se dependent glutathione peroxidase (GSH-Px) and the Zn/Cu dependent superoxide dismutase (SOD). The plasma concentrations (mean +/- SD) for Se, Cu and Zn were 0.84 +/- 0.10 micromol/l, 15.6 +/- 2.78 micromol/l and 12.6 +/- 1.80 micromol/l, resp., and for non protein-bound and protein bound MDA 0.27 +/- 0.07 micromol/l and 1.11 +/- 0.25 micromol/l, resp. The activity of GSH-Px in plasma and erythrocytes was 130 +/- 20.8 U/l and 19.8 +/- 4.18 U/mg Hb, resp. and of SOD in erythrocytes 3,159 +/- 847.2 U/g Hb. In plasma positive correlations have been found between Se concentrations and GSH-Px activities (p < 0.002, r = 0.31) and between GSH-Px activities and concentrations of non protein-bound MDA (p = 0.004, r = 0.28). A negative correlation has been observed between GSH-Px activities in plasma and in erythrocytes. The higher the concentrations of Cu in erythrocytes, the higher were the activities of SOD (p = 0.03, r = 0.22) and GSH-Px in erythrocytes (r = 0.26, p = 0.01), while an increasing activity of GSH-Px in these cells correlated with a decreasing concentration of non protein-bound MDA (r = -0,31, p = 0.002). An increase in BMI was connected with an increase in protein-bound MDA and a decrease in GSH-Px activities in pLasma (p = 0.002 and r = 0.23). As the results demonstrate, Se and Cu concentrations in erythrocytes can improve the trace element dependent antioxidative status.     

Superoxide dismutase, glutathione peroxidase, catalase, and lipid peroxidation in the major organs of the aging rats

Glutathione peroxidase (GSh-Px), superoxide dismutase (SOD), catalase (CAT) activities and malon-dialdehyde (MDA) content were determined in heart, liver, kidney and brain of rats. Two different age groups (4 months; 24 months) were considered. GSH-Px and SOD activities decrease significantly for the aged liver and kidney. During aging, the activity of catalase increase in cardiac muscle and, in contrast, decrease in other organs. Lipids peroxidation, expressed in term of MDA formation, decrease in all the organs of the aged rats. The results indicate that: 1) the liver and kidney antioxidative defense decrease with age; 2) the enzymatic activities evolve in a different manner for different enzymes and organs. Furthermore, the results suggest that there is not any correlation between the SOD, CAT, and GSH-Px activities and the peroxidative status of the organs; thus, the age-related increase in the MDA content proposed as a criterion of aging process should be considered with caution.     

镉对长江华溪蟹精巢抗氧化酶活性及脂质过氧化的影响

在实验条件下,将健康性成熟雄性长江华溪蟹Sinopotamon yangtsekiense暴露于0、7.25、14.5、29、58和116 mg/L浓度的镉(Cd2+)溶液中,分别于1 d、3 d、5 d和7 d时测定精巢中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氧酶(CAT)活性及脂质过氧化产物丙二醛(MDA)的含量.结果显示,不同时间段3种酶活性和MDA含量均具有浓度和时间效应关系,表明急性镉暴露对精巢有明显的毒性作用,其作用机制与抗氧化酶活力变化和脂质过氧化加剧有关.     

Alterations of serum selenium, zinc, copper, and iron concentrations and some related antioxidant enzyme activities in patients with cutaneous leishmaniasis

The aim of this study was to measure the alterations in serum selenium (Se), copper (Cu), zinc (Zn), and iron (Fe) concentrations and their carrier proteins, ceruloplasmin (Cp), transferrin (Tf) albumin, and related antioxidant enzyme activities, erythrocyte Cu-Zn Superoxide dismutase (Cu-Zn SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities in patients with cutaneous leishmaniasis (CL). Erythrocyte Cu-Zn SOD activities, serum Cu concentrations, and Cp levels were found to be significantly higher in the patients group than those of controls. However, GSH-Px and CAT activities and Se, Zn, Fe, and Tf levels were lower in patients than in the control subjects. There were positive important correlation’s between Cu-Zn SOD and Cp, Cu-Zn SOD and Cu, Cp and Cu, GSH-Px and Se, and Fe and CAT in the patients group. Our results showed that serum essential trace elements Se, Zn, Cu, and Fe concentrations and their related enzymes Cu-Zn SOD, GSH-Px, and CAT activities change in CL patients. The changes may be a part of defense strategies of organism and are induced by the hormonelike substances.     

Oxidative Stress Parameters in Different Brain Structures Following Lateral Fluid Percussion Injury in the Rat

Free radicals mediated damage of phospholipids, proteins and nucleic acids results in subsequent neuronal degeneration and cell loss. Aim of this study was to evaluate the existence of lipid and protein oxidative damage and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in various rat brain structures 24 h after lateral fluid percussion brain injury (LFPI). Parietal cortex, hippocampus, thalamus, entorhinal cortex, and cerebellum from the ipsilateral hemisphere were processed for analyses of the thiobarbituric acid reactive substances (TBARS) and oxidized protein levels as well as for the SOD and GSH-Px activities. Immunohistochemical detection of oxidized proteins was also performed. Results of our study showed that LFPI caused significant oxidative stress in the parietal cortex and hippocampus while other brain regions tested in this study were not oxidatively altered by LFPI. GSH-Px activities were significantly increased in the parietal cortex and hippocampus, while the SOD activities remained unchanged following LFPI in all regions investigated.     

Protective effect of caffeic acid phenethyl ester (CAPE) on lipid peroxidation and antioxidant enzymes in diabetic rat liver

The aim of this study was to examine the effect of caffeic acid phenethyl ester (CAPE) on lipid peroxidation (LPO) and the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the liver of streptozotocin (STZ)-induced diabetic rats. Twenty-seven rats were randomly divided into three groups: group I, control non-diabetic rats (n = 9); group II, STZ-induced, untreated diabetic rats (n = 8); group III, STZ-induced, CAPE-treated diabetic rats (n = 10), which were intraperitoneally injected with CAPE (10 microM kg(-1) day(-1)) after 3 days followed by STZ treatment. The liver was excised after 8 weeks of CAPE treatment, the levels of malondialdehyde (MDA) and the activities of SOD, CAT, and GSH-Px in the hepatic tissues of all groups were analyzed. In the untreated diabetic rats, MDA markedly increased in the hepatic tissue compared with the control rats (p < 0.0001). However, MDA levels were reduced to the control level by CAPE. The activities of SOD, CAT, and GSH-Px in the untreated diabetic group were higher than that in the control group (p < 0.0001). The activities of SOD and GSH-Px in the CAPE-treated diabetic group were higher than that in the control group (respectively, p < 0.0001, p < 0.035). There were no significant differences in the activity of CAT between the rats of CAPE-treated diabetic and control groups. Rats in the CAPE-treated diabetic group had reduced activities of SOD and CAT in comparison with the rats of untreated diabetic group (p < 0.0001). There were no significant differences in the activity of GSH-Px between the rats of untreated diabetic and CAPE-treated groups. It is likely that STZ-induced diabetes caused liver damage. In addition, LPO may be one of the molecular mechanisms involved in STZ-induced diabetic damage. CAPE can reduce LPO caused by STZ-induced diabetes.