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1.
1991年从沈阳地区茄子花叶病叶片中分离到了SY-Is分离物。经汁液摩擦接种7个科16种植物,这一分离物可侵染4个科8种植物,但不侵染黄瓜,西葫芦和蚕豆,也不能经桃蚜和萝卜蚜传播。该分离物体外抗性的致死温度(TIP)为90-95℃;稀释限点(DEP)为10^-^6-10^-^7;体外保毒期(L)为一个月以上。光学显镜微镜下可见晶状胞质内含体。叶滴法电镜观察可见大小为±300×18nm的杆状病毒粒子 相似文献
2.
田间采集辽宁地区烟叶脉坏死病标样所得分离物,在测定的12个科35种植物中只侵染茄科的一些烟草品种及洋酸浆(physalisfloridana),可由桃蚜(Myzuspersicae)传播。病叶汁液稀释限点(DEP)为10 ̄(-2)-v10 ̄(-3);失毒温度(TIP)为55一60℃;体外保毒期(L)为48-72小时。病毒粒体形态呈线条状720×12nm,病叶脉坏死部细胞质中含风轮状内含体。病毒提取物的紫外最大吸收为265nm,最小吸收为245nm,A_(280)/A_(260)为0.82。该病毒分离物与PVY ̄0抗血清呈阳性反应。以病毒RNA为模板,按国外报道的PVY ̄N序列合成引物经逆转录合成cDNA。用PCR扩增出约0.80kb的CP基因片段,将这一片段插入载体pGEM7Z-f(+)中转化E.coliDH5a菌株得到了CP基因的克隆。cDNA序列分析表明,和国外报道的PVY ̄N序列同源性极高,初步表明引起辽宁地区烟叶脉坏死病的毒原为PVY ̄N。 相似文献
3.
侵染番茄的番茄花叶病毒的研究 总被引:10,自引:0,他引:10
从种传番茄苗中获得一病毒分离物To-Sl,人工摩擦接种7科24种植物,To~Sl能侵染4科15种植物,在番茄上产生花叶,在白肋烟上为局部枯斑。To-Sl的钝化温度为85~90℃,稀释限点为10 ̄(-6)~10 ̄(-7).体外保毒期在一个月以上。病毒粒体杆状,长度主要分布于281~300nm之间,平均长度288nm。病毒衣壳蛋白亚基只有一条多肽链,分子量为21kDa。dsRNA分析测得其基因组长度为6.4kb。琼胎糖双扩散和胶内交叉吸附试验证明,To-Sl与TMV有血清关系,但有一定的差异,病毒粒体电泳分析也表明To-Sl与TMV粒体有差异。在交叉保护试验中,TMV和To-Sl之间均无保护作用。根据以上试验结果To-Sl被鉴定为番茄花叶病毒。这是我国首次系统报道番茄上番茄花叶病毒的侵染。 相似文献
4.
新疆辣椒轻微斑驳病毒的分离鉴定 总被引:8,自引:0,他引:8
从石河子露地辣椒病株上分离到一株病毒分离物,引起辣椒轻微斑驳。该分离物经人工接种能侵染5科9种植物。感染的辣椒种子可传毒,但桃蚜不能传播。P-2致死温度90℃,稀释限点10^-9,体外保毒期50天以上。病毒颗粒呈杆状,稍弯曲,大小为295±10×17nm;辣椒病组织超薄切片可见大量以平行或交叉排列的病毒集结体。该分离物与辣椒轻微斑驳病抗血清呈阳性反应。在辣椒上与TMV无任何交叉保护。SDS-聚丙烯 相似文献
5.
引起番茄坏死病的黄瓜花叶病毒TN分离物的研究 总被引:3,自引:0,他引:3
从南京郊区田间番茄坏死病株中分离出黄瓜花叶病毒TN分离物,经人工接种10科39种植物,能侵染其中的8科26种,接种番茄,辣椒和普通烟都引起坏死症状。TN分离物的失毒温度为50℃,稀释限点5×10 ̄(-3)-5×10 ̄(-4),体外存活期1-2天。蚜虫以非持久性方式传毒,经免疫双扩散测定,TN分离物与黄瓜花叶病毒抗血清呈阳性反应。用差速离心提纯的病毒粒子经电镜观察为廿面体,直径为28nm。SDS-PAGE分析病毒外壳蛋白为单一组份,分子量27500D。病毒核酸组份分析发现,该病毒分离物含有CMV基因组核酸和一个低分子量的卫星RNA。以上结果同已报道的引起番茄坏死的带卫星RNA的黄瓜花叶病毒株系一致(Kearney,C.M.1990;Jorda,C.1992)。本文还讨论了该病害的发生和流行。 相似文献
6.
采用PEG沉淀和差速离心的方法提纯雀麦花叶病毒的G和T分离物。利用蛋白酶K和两相酚法制备雀麦花叶病毒的总RNAs。将G和T分离物RNAs进行琼脂糖凝胶电泳,结果发现Br-MV-G除含有正常的RNA组分外,还出现了另一新的RNA_(3b)组分,其分子量为0.50×10 ̄6。RNA_(3b)只出现在大麦寄主中,而在昆诺基上缺失。RNA_(3b)仅依靠于其来源的G分离物的RNAs进行复制。以RNA_(3b)为模板合成 ̄(32)P-cDNA探针,和BrMV-G分离物的RNAs进行分子杂交试验表明:RNA_(3b)属RNA_3的缺陷型组分,它依赖于BrMV-G-RNA_3的帮助才能在大麦寄主中复制。RNA_(3b)的出现和缺失对BrMV的症状表现没有影响。 相似文献
7.
烟草花叶病毒(普通株中国分离物)及其弱毒苗—N14(番茄株)组织 … 总被引:2,自引:0,他引:2
用双脱氧未端经终止法对侵染性烟草共现毒普通株中国分离物(TMV-virlgar,Chinese lsoblate,TMV-Cv)和番茄株弱毒轩TMV-N14(Attenuated TMV vaccine strain)基因组cDNAs的核苷酸全序列进行了测定,并分析和比较了其基因组的结构和特征。结果表明:普通株基因组(Genbank接收号:AF165190)为6395个核苷酸:4个功能性开放阅读框 相似文献
8.
从宁夏患丛根病甜菜的病根中分离到一种球状病毒粒子,直径约为28nm。提纯病毒的紫外扫描呈典型的核蛋白曲线,最大吸收为260nm,最小吸收为240nm,A_(260)/A_(280)=1.30。寄主范围广,能侵染茄科、豆科、藜科、葫芦科、番杏科等17种植物。病毒的TDP为65℃,DEP为10 ̄(-3),体外存活期6-7天。有较强的免疫原性。病毒的抗血清与烟草环斑病毒(TRSV)之间产生明显的沉淀线。经初步鉴定认为该分离物是烟草环斑病毒。 相似文献
9.
安徽虫瘟霉菌株的强毒杀蚜应与侵染速率 总被引:5,自引:2,他引:3
报道安徽虫瘟霉(Zoophthora anhuiensis)菌株F97028对桃蚜(Myzus persicae)的强毒杀蚜活性。以7个孢子剂量(0.4 ̄10.4个孢子/mm^2)接种2 ̄3龄若蚜(52 ̄86头/剂量),连续观察7d,所获数据经时间-剂量-死亡率模型模拟分析,接种后第3 ̄7d的LD50分别为34.8、87、1.5、0.7和0.4个孢子/mm^2;在所有剂量范围内LT50为2.9 ̄6 相似文献
10.
侵染新疆甜菜的两种病毒分离物的研究 总被引:2,自引:0,他引:2
在新疆甜菜的根部和叶片分离到两个球状病毒分离物。从病毒形态、生物学及物化性质等研究结果证明这两个分离物实是一种病毒。病毒粒体力20面体,直径约28nm,回接到甜菜上产生环斑和沿脉线纹最后形成坏死斑。此外还侵染苋色藜,昆诺阿黎,番杏,菠菜等,引起局部坏死斑。在普通烟,心叶烟,菜豆,黄瓜,蕃茄上无症状侵染,病毒致死温度为70℃,10分钟。体外存括期13天以上,冻干病叶7年后仍有侵染力。通过PEG沉淀、差速离心、琼脂糖柱层析及蔗糖梯度离心,可得到较高浓度的纯化病毒,病毒的紫外吸收最低值在245nm,最高值在260nm。经SDS-PAGE测定,病毒外壳蛋白的分子量为2.7×10 ̄4道尔顿,病毒基因组核酸为三个组份,分子量分别为3.08kb,1.28kb和0.85kb。在琼脂双扩散实验中,能与番茄黑环病毒(TBRV)抗血清产生较弱的沉淀线,与黄瓜花叶病毒(CWV)、烟草环斑病毒(TRSV)、烟草坏死病毒(TNV)、香石竹环斑病毒(CaRSV)的抗血清不发生反应。 相似文献
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M. L. Fisher M. M. Kyle 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(2):204-212
We have examined the genetics of systemic resistance in Phaseolus vulgaris to azuki bean mosaic virus (AzMV) and cowpea aphid-borne mosaic virus (CABMV) and the relationship of this resistance to a phenotypically similar resistance to watermelon mosaic virus (WMV) and soybean mosaic virus (SMV). In P. vulgaris cv Great Northern 1140 (GN1140), resistance to SMV and WMV has been attributed to the genes Smv and Wmv, respectively, which have been shown to segregate as a unit. Systemic resistance to AzMV is conferred by two incompletely dominant alleles, Azm1 and Azm2, at unlinked loci. At least three resistance alleles must be present at these two loci for systemic resistance to be expressed in the plant. Systemic resistance to CABMV in GN 1140 is conditioned by a dominant allele that has been designated Cam2. Under some environmental conditions, a recessive allele at an unlinked locus, cam3, also controls a resistant response to CABMV. Resistance to AzMV and CABMV does not assort independently from Wmv/Smv, but also does not consistently cosegregate, suggesting that perhaps in each case one of the factors involved in resistance is associated with Smv/Wmv. 相似文献
13.
Summary A comparison was made of the amino acid sequences of the proteins encoded by RNAs 1 and 2 of alfalfa mosaic virus (A1MV) and brome mosaic virus (BMV), and the 126K and 183K proteins encoded by tobacco mosaic virus (TMV). Three blocks of extensive homology of about 200 to 350 amino acids each were observed. Two of these blocks are located in the A1MV and BMV RNA 1 encoded proteins and the TMV encoded 126K protein; they are situated at the N-terminus and C-terminus, respectively. The third block is located in the A1MV and BMV RNA 2 encoded proteins and the C-terminal part of the TMV encoded 183K protein. These homologies are discussed with respect to the functional equivalence of these putative replicase proteins and a possible evolutionary connection between A1MV, BMV and TMV. 相似文献
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O. A. Ariyo M. Koerbler A. G. O. Dixon G. I. Atiri S. Winter 《Journal of Phytopathology》2005,153(4):226-231
Several begomovirus species and strains causing Cassava mosaic disease (CMD) have been reported from cassava in Africa. In Nigeria, African cassava mosaic virus (ACMV) was the predominant virus in this important crop, and East African cassava mosaic virus (EACMV), first reported from eastern Nigeria in 1999, was also found occasionally. A survey was conducted in 2002 to resolve the diversity of the virus types present in cassava in Nigeria and to further understand the increasing complexity of the viruses contributing to CMD. A total of 234 leaf samples from cassava with conspicuous CMD symptoms were collected in farmers’ fields across different agroecological zones of Nigeria and subjected to polymerase chain reaction (PCR) with type‐specific primers. In addition and, to provide a full characterization of the viruses present, DNA‐A genome components of several viruses and informative genome fragments were sequenced. In Nigeria, ACMV proved to be the dominant virus with 80% of all samples being positive for ACMV. The East African cassava mosaic Cameroon virus (EACMCV) prevalent in Cameroon and Ivory Coast was detected in single infections (2%) and in mixed infections (18%) with ACMV. There was no indication for other virus strains of EACMV present in the country. The EACMCV samples collected showed a high nucleotide sequence identity >98% and resembled the described sequence of a Cameroon isolate (EACMCV‐CM) more than an Ivory Coast isolate, EACMCV‐CM[CI]. Evidence is provided that the EACMCV has reached epidemiological significance in Nigeria. 相似文献
16.
应用斑点法检测了病叶粗汁液中的芜菁花叶病毒(TuMV)、大豆花叶病毒(sMV)和黄瓜花叶病毒(CMV),病叶粗汁液可被检测的最大稀释度分别为1:5120、1:2560和1:1280。提纯的大豆花叶病毒和黄瓜花叶病毒可检测的最低限量分别为1.7ng和1.2ng。以牛血清白蛋白、吐温和聚乙烯吡咯啉酮作封闭液,均可获得满意的结果。应用斑点法检测芜菁花叶病毒和大豆花叶病毒时,其抗血清稀释1:500倍可获得满意效果,稀释2000倍仍可用于检测。 相似文献
17.
B OWOR J P LEGG G OKAO-OKUJA R OBONYO M W OGENGA-LATIGO 《The Annals of applied biology》2004,145(3):331-337
A study was carried out to assess the effect of different cassava mosaic geminiviruses (CMGs) occurring in Uganda on the growth and yield of the susceptible local cultivar ‘Ebwanateraka’. Plants infected with African cassava mosaic virus (ACMV), ‘mild’ and ‘severe’ strains of East African cassava mosaic virus‐Uganda (EACMV‐UG2) and both ACMV and EACMV‐UG2 were grown in two experiments in Kabula, Lyantonde in western Uganda. The most severe disease developed in plants co‐infected with ACMV and EACMV‐UG2 and in those infected with the ‘severe’ form of EACMV‐UG2 alone; disease was least severe in plants infected with the ‘mild’ strain of EACMV‐UG2. ACMV‐infected plants and those infected with the ‘mild’ strain of EACMV‐UG2 were tallest in the 1999–2000 and 2000–2001 trials, respectively; plants dually infected with ACMV and EACMV‐UG2 were shortest in both trials. Plants infected with ‘mild’ EACMV‐UG2 yielded the largest number and the heaviest tuberous roots followed by ACMV and EACMV‐UG2 ‘severe’, respectively, whilst plants dually infected with ACMV and EACMV‐UG2 yielded the least considering the two trials together. Reduction in tuberous root weight was greatest in plants dually infected with ACMV and EACMV‐UG2, averaging 82%. Losses attributed to ACMV alone, EACMV‐UG2 ‘mild’ and EACMV‐UG2 ‘severe’ were 42%, 12% and 68%, respectively. Fifty percent and 48% of the plants infected with both ACMV and EACMV‐UG2 gave no root yield in 1999–2000 and 2000–2001, respectively. These results indicate that CMGs, whether in single or mixed infections, reduce root yield and numbers of tuberous roots produced and that losses are substantially increased following mixed infection. 相似文献
18.
Angela O. Eni Patricia Ogunsanya Taiwo Oviasuyi Jacqueline d’A Hughes 《Archives Of Phytopathology And Plant Protection》2013,46(16):1958-1965
Cowpea plays a key nutritional role in the diet of the Nigerian people. Viral diseases are a major limitation to cowpea production worldwide, and thus, constant viral surveillance is crucial for monitoring and management purposes. In this study, cowpea leaf samples from fields in three northern Nigeria states, Kano, Kaduna and Niger, were tested to determine the status of six common viruses previously reported in these cowpea-producing states following the release of virus-resistant varieties. Cowpea aphid-borne mosaic virus (CABMV), Blackeye cowpea mosaic virus (BICMV), Cowpea mottle virus, Southern bean mosaic virus and Cucumber mosaic virus (CMV) were detected. Cowpea yellow mosaic virus, which was previously reported in all three states, was not detected in any of the samples tested, while CMV that was previously regarded as unimportant to cowpea production in Nigeria had the highest incidence in all three states, and the overall highest incidence of 58.8%, while CABMV had the lowest incidence (7.5%). CMV was also present in seven of the ten mixed infection combinations detected. Dual infection of CMV and BICMV, which often results in cowpea stunt, the most devastating cowpea disease in the USA, was the most frequently detected mixed infection (28.1%) and was detected in all three states. This observed elevation in CMV infection in cowpea must be closely monitored and swiftly managed to avert possible devastating crop yield losses. 相似文献
19.
The amino acid sequences of the non-structural protein (molecular weight 35,000; 3a protein) from three plant viruses — cucumber
mosaic, brome mosaic and alfalfa mosaic have been systematically compared using the partial genomic sequences for these three
viruses already available. The 3a protein of cucumber mosaic virus has an amino acid sequence homology of 33.7% with the corresponding
protein of brome mosaic virus. A similar protein from alfalfa mosaic virus has a homology of 18.2% and 14.2% with the protein
from brome mosaic virus and cucumber mosaic virus, respectively. These results suggest that the three plant viruses are evolutionarily
related, although, the evolutionary distance between alfalfa mosaic virus and cucumber mosaic virus or brome mosaic virus
is much larger than the corresponding distance between the latter two viruses. 相似文献
20.
从杭州地区呈现玉米矮花叶典型症状的玉米病组织中提纯得到大量线状病毒粒子,大多数长度为750nm。病组织中含有大量风轮状内含体和板状集结体,病毒外壳蛋白为33.6kD。病毒RNA1 3'端序列(1.8kb)与甘蔗茶花经叶病毒(SCMV)同源性最高,达71.5%-99.1%,与高梁花叶病毒(SrMV)同源性次之,为67.8%-68.5%,与玉米矮花叶病毒(MDMV)同泊性最低,仅为38.4%-48.4%,从而初步认为此病害由SCMV引起。根据已发表的SCMV外壳蛋白氨基酸序列作亲缘性分析,表明SCMV可分为美国、南非、澳大利亚、德国和中国三大类。 相似文献