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N. M. Endersby A. A. Hoffmann S. W. McKechnie & A. R. Weeks 《Entomologia Experimentalis et Applicata》2007,122(3):253-263
Recent studies suggest that populations of the pest moth Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) may be genetically differentiated over short distances and time periods within Queensland, Australia. To test for genetic structure in another region of Australia, we characterized population differentiation in Victorian samples of H. armigera using eight microsatellite loci. We found no evidence of genetic structure among samples from different locations or among samples collected at different times. Moreover, Victorian samples were not differentiated from other samples of H. armigera from Queensland and New Zealand. All samples showed substantial deviations from Hardy–Weinberg equilibrium, suggesting a high frequency of null alleles typically found in microsatellites of Lepidoptera. These results indicate that populations of H. armigera are not strongly structured among regions in south‐eastern Australia. 相似文献
3.
C. J. Anderson W. T. Tay A. McGaughran K. Gordon T. K. Walsh 《Molecular ecology》2016,25(21):5296-5311
Helicoverpa armigera is a major agricultural pest that is distributed across Europe, Asia, Africa and Australasia. This species is hypothesized to have spread to the Americas 1.5 million years ago, founding a population that is at present, a distinct species, Helicoverpa zea. In 2013, H. armigera was confirmed to have re‐entered South America via Brazil and subsequently spread. The source of the recent incursion is unknown and population structure in H. armigera is poorly resolved, but a basic understanding would highlight potential biosecurity failures and determine the recent evolutionary history of region‐specific lineages. Here, we integrate several end points derived from high‐throughput sequencing to assess gene flow in H. armigera and H. zea from populations across six continents. We first assemble mitochondrial genomes to demonstrate the phylogenetic relationship of H. armigera with other Heliothine species and the lack of distinction between populations. We subsequently use de novo genotyping‐by‐sequencing and whole‐genome sequences aligned to bacterial artificial chromosomes, to assess levels of admixture. Primarily, we find that Brazilian H. armigera are derived from diverse source populations, with strong signals of gene flow from European populations, as well as prevalent signals of Asian and African ancestry. We also demonstrate a potential field‐caught hybrid between H. armigera and H. zea, and are able to provide genomic support for the presence of the H. armigera conferta subspecies in Australasia. While structure among the bulk of populations remains unresolved, we present distinctions that are pertinent to future investigations as well as to the biosecurity threat posed by H. armigera. 相似文献
4.
Five microsatellite loci of Helicoverpa armigera were isolated from a partial genomic library screened by oligonucleotide probes. Primers were designed to detect allelic variability and heterozygosity in 60 individuals collected from different host species. All loci were found to be polymorphic, have 8–11 alleles with expected heterozygosity ranging from 0.81 to 0.88. Our results indicate that the five microsatellite loci could provide valuable markers for population genetic and ecological studies of the cotton bollworm. 相似文献
5.
Tandemly duplicated actin genes have been isolated from a Helicoverpa armigera genomic library. Sequence comparisons with actin genes from other species suggest they encode cytoplasmic actins, being most
closely related to the Bombyx mori A3 actin gene. The duplicated H. armigera actin genes, termed A3a and A3b, share 98.3% nucleotide sequence identity over their entire putative coding region. Analysis
of the distribution of nucleotide differences shows the first 763 bp are identical between the two coding regions, with the
18 nucleotide changes occurring in the remaining 366 bp. This observation suggests a gene conversion event has taken place
between the duplicated H. armigera A3a and A3b actin genes. Translation of the open-reading frames indicates the products of these genes are identical, apart
from a single amino acid difference at codon 273. Polymerase chain reaction and northern blot analysis have shown both H. armigera A3a and A3b genes are expressed during pupal development and in the brain of newly eclosed adults. A region 5′ of the H. armigera A3a actin gene start codon has been identified which contains regulatory sequences commonly found in the promoter region
of actin genes, including TATA, CAAT, and CArG motifs.
Received: 10 January 1996 / Accepted: 12 March 1996 相似文献
6.
Mark G. Teese Peter M. Campbell Colin Scott Karl H.J. Gordon Adam Southon Daniel Hovan Charles Robin Robyn J. Russell John G. Oakeshott 《Insect biochemistry and molecular biology》2010,40(1):1-16
Some of the resistance of Helicoverpa armigera to conventional insecticides such as organophosphates and synthetic pyrethroids appears to be due to metabolic detoxification by carboxylesterases. To investigate the H. armigera carboxyl/cholinesterases, we created a data set of 39 putative paralogous H. armigera carboxyl/cholinesterase sequences from cDNA libraries and other sources. Phylogenetic analysis revealed a close relationship between these sequences and 70 carboxyl/cholinesterases from the recently sequenced genome of the silkworm, Bombyx mori, including several conserved clades of non-catalytic proteins. A juvenile hormone esterase candidate from H. armigera was identified, and B. mori orthologues were proposed for 31% of the sequences examined, however low similarity was found between lepidopteran sequences and esterases previously associated with insecticide resistance from other insect orders. A proteomic analysis of larval esterases then enabled us to match seven of the H. armigera carboxyl/cholinesterase sequences to specific esterase isozymes. All identified sequences were predicted to encode catalytically active carboxylesterases, including six proteins with N-terminal signal peptides and N-glycans, with two also containing C-terminal signals for glycosylphosphatidylinositol anchor attachment. Five of these sequences were matched to zones of activity on native PAGE at relative mobility values previously associated with insecticide resistance in this species. 相似文献
7.
Ming-Hui Jin Yu-Tao Xiao Ying Cheng Jie Hu Chao-Bin Xue Kong-Ming Wu 《Insect Science》2019,26(6):1029-1036
Helicoverpa armigera, cotton bollworm, is one of the most disastrous pests worldwide, threatening various food and economic crops. Functional genomic tools may provide efficient approaches for its management. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system, dependent on a single guide RNA (sgRNA), has been used to induce indels for targeted mutagenesis in cotton bollworm. However, genomic deletions may be more desirable to disrupt the function of noncoding genes or regulatory sequences. By injecting two sgRNAs with Cas9 protein targeting different exons, we obtained predictable genomic deletions of several hundred bases. We achieved this type of modification with different combinations of sgRNA pairs, including HaCad and HaABCC2. Our finding indicated that CRISPR/Cas9 can be used as an efficient tool to engineer genomes with chromosomal deletion in H. armigera. 相似文献
8.
Monitoring of four major lepidopteran pests in Korean cornfields and management of Helicoverpa armigera
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Lepidopterans such as Helicoverpa armigera are emerging pests of corn in Korea, causing huge yield losses and deteriorating the quality of the corn crop. We monitored four major lepidopteran pests in major corn growing areas of Korea by employing sex pheromone traps from 2012 to 2015 to understand population dynamics. H. armigera, Ostrinia furnacalis, Spodoptera litura, Spodoptera exigua and Mythimna separata were collected from April to October. Among these species, H. armigera was found to be the main pest based on crop damage intensity (80–90% of the total damage). H. armigera was generally observed during the earing stage of corn and would migrate to other fields during September to October for overwintering. We also tested select insecticides against larvae of H. armigera in the laboratory. The most effective insecticide was indoxacarb, which was applied at specific times to manage H. armigera in cornfields. Optimal timing of spraying was estimated at about 70 days after planting (earing season) to control for H. armigera. Additionally, two species of parasitoid (Therion circumflexum and Ophioninae sp.) were identified from H. armigera. However, more extensive surveys are needed to organize a control program based on natural enemies. 相似文献
9.
Xin-Cheng Zhao Yun-Hua Yan Chen-Zhu Wang 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2006,192(10):1037-1047
Two sibling species, Helicoverpa assulta and Helicoverpa armigera both use (Z)-9-hexadecenal and (Z)-11-hexadecenal as their sex pheromone components but in almost reversed ratios, 93:7 and 3:97, respectively. H. assulta and H. armigera males performed upwind flight in response to the H. assulta sex pheromone blend (93:7). H. armigera responded strongly to the H. armigera blend (3:97), whereas H. assulta males remained inactive upon exposure to this blend. Both species gave clear dose-dependent electrophysiological responses to (Z)-11-hexadecenal. However, (Z)-9-hexadecenal evoked strong dose-dependent electrophysiological responses in H. assulta males but not in H. armigera. The two male F1 hybrids exhibited similar behavioral responses to two sex pheromone blends and electrophysiological responses to two pheromone components as H. armigera males. This indicated that H. armigera genes appear dominant in determining the behavioral response and electrophysiological responses. Behavioral and electrophysiological responses of backcrosses of male F1 hybrids (H. armigera female × H. assulta male) with female H. assulta and H. armigera were close to that of H. assulta and H. armigera, respectively. However, backcrosses of female F1 hybrids (H. assulta female × H. armigera male) with male H. assulta and H. armigera showed reduced behavioral responses but normal electrophysiological responses compared to males of the respective parental line. 相似文献
10.
Manish Roorkiwal Atul Grover Prakash C. Sharma 《Molecular genetics and genomics : MGG》2009,282(2):205-215
Studies on microsatellite distribution and divergence in related genomes contribute towards understanding of genome evolution
in eukaryotes. Despite the availability of whole genome sequences of four rice genomes, occurrence and significance of microsatellites
in the rice genome has remained a relatively unexplored area of research. We have aligned genomes of two rice subspecies i.e.
indica and japonica to understand the trends of microsatellite conservation and divergence in the rice genome. Nearly 62% of the indica microsatellites were also found in the japonica genome. Occurrence of microsatellites showed a negative association with that of retrotransposons. Microsatellites repeat
unit length and sequence showed direct influence on the microsatellite locus length. Further, microsatellite allele length
was also influenced by the sequence characteristics of the neighbouring regions. CCG repeats were most conserved microsatellite
sequences across the different syntenic regions in the two rice genomes and often showed association with CpG islands. Our
study suggested that microsatellite distribution is not only governed by a balance between replication slippage and point
mutations as proposed earlier, but also by the microsatellite motif sequence and characteristics of microsatellite neighbouring
regions in the genome. Thus, this study is likely to prove an important reference for understanding the process of microsatellite
evolution and dynamics in the two rice subspecies. 相似文献
11.
Francis E. Hinds Robert L. Close Mary T. Campbell Peter B. S. Spencer 《Molecular ecology resources》2002,2(1):42-44
The water rat (Hydromys chrysogaster) is well adapted to a semiaquatic life and is endemic to dispersed regions of Australia and New Guinea. To analyse the genetic diversity of water rat populations, polymorphic microsatellite markers were developed. A partial genomic library was screened for microsatellite sequences. Following isolation of the microsatellite sequences, primers were designed to amplify seven loci and of these loci, five were polymorphic. The sample tested for polymorphisms came from areas across Australia and New Guinea. Between three and 13 alleles were detected for each locus. In addition the primers amplified two loci in Mus musculus and Rattus rattus. 相似文献
12.
Rogrio Martins Gonalves Thiago Mastrangelo Jos Carlos Verle Rodrigues Daniel Fernando Paulo Celso Omoto Alberto Soares Corrêa Ana Maria Lima de Azeredo‐Espin 《Ecology and evolution》2019,9(13):7378-7401
In 2013, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) was officially declared as present in Brazil and, after two years, the species was detected in the Caribbean and North America. Information on genetic features and accurate distribution of pests is the basis for agricultural protection policies. Furthermore, such knowledge is imperative to develop control strategies, understand the geographical range, and genetic patterns of this species in the Americas. Here, we carried out the widest sampling of H. armigera in the South American continent and Puerto Rico, after we estimated the diversity, demographic parameters, and genetic structure. The Internal Transcribed Spacer 1 (ITS1) nuclear marker was used to investigate the presence of putative hybrids between H. armigera and H. zea, and they were observed at a frequency of 1.5%. An ABC analysis, based in COI gene fragment, suggested Europe as the origin of South America specimens of H. armigeraand following a movement northward through the Caribbean. Three mtDNA genes and three nDNA markers revealed high genetic diversity distributed without the defined population structure of H. armigera in South America. Most of the genetic variation is within populations with a multidirectional expansion of H. armigera among morphoclimatic regions. High genetic diversity, rapid population expansion, and hybridization have implications for pest management since they suggest that adaptive alleles are spread through wide areas in South America that favor rapid local adaptation of H. armigera to new and disturbed environments (e.g., in agricultural areas). 相似文献
13.
The relatively low susceptibility ofHelicoverpa armigera to CrylAc, its history of resistance to chemical insecticides and the seasonal decline in expression of CrylAc in transgenic cotton necessitated the development of cotton expressing two insecticidal proteins to provide sustainable control of this multinational pest. To manage the resistance issue, it was essential that the second insecticidal protein have a significantly different mode of action to CrylAc. A common feature of resistance to CrylA proteins in several species as well as H. armigera has been a change in the binding site. A study of binding sites for some Cry proteins in the brush border membrane vesicles (BBMV) ofH. armigera and Helicoverpa punctigera was undertaken. The binding affinity for CrylAc was higher than for CrylAb, matching their relative toxicities, and CrylAc and CrylAb were found to share at least one binding site in both I-1. armigera and I-1. punctigera. However Cry2Aa did not compete with CrylAc for binding and so could be used in transgenic cotton in combination with CrylAc to control H. armigera and manage resistance. Variation in the susceptibilities of three different H. armigera strains to CrylAc correlated with the parameter Bmax/Kcom. 相似文献
14.
De-Xing Zhang Lu-Na Yan Ya-Jie Ji Le Kang Godfrey M. Hewitt Zu-Shi Huang 《Molecular ecology resources》2003,3(4):483-486
Eight polymorphic di‐ and trinucleotide microsatellite loci suitable for population genetic analysis were developed in Locusta migratoria from a partial phagemid genomic library enriched for microsatellite inserts. The expected heterozygosity at these loci ranges from 0.45 to 0.97, with the observed allele numbers varying between nine and 45. The overall microsatellite cloning efficiency in L. migratoria is 14%, suggesting that in migratory locusts, microsatellite sequences are abundant and should provide a valuable and easily accessible source of nuclear markers for genetic studies. These microsatellite loci were highly Locusta‐specific, with only very limited cross‐species applicability. 相似文献
15.
Gajanan T Behere Wee Tek Tay Derek A Russell David G Heckel Belinda R Appleton Keshav R Kranthi Philip Batterham 《BMC evolutionary biology》2007,7(1):117
Background
Helicoverpa armigera and H. zea are amongst the most significant polyphagous pest lepidopteran species in the Old and New Worlds respectively. Separation of H. armigera and H. zea is difficult and is usually only achieved through morphological differences in the genitalia. They are capable of interbreeding to produce fertile offspring. The single species status of H. armigera has been doubted, due to its wide distribution and plant host range across the Old World. This study explores the global genetic diversity of H. armigera and its evolutionary relationship to H zea. 相似文献16.
E.‐M. You K.‐F. Liu S.‐W. Huang M. Chen M. L. Groumellec S.‐J. Fann H.‐T. Yu 《Animal genetics》2010,41(4):365-376
The linkage maps of male and female tiger shrimp (P. monodon) were constructed based on 256 microsatellite and 85 amplified fragment length polymorphism (AFLP) markers. Microsatellite markers obtained from clone sequences of partial genomic libraries, tandem repeat sequences from databases and previous publications and fosmid end sequences were employed. Of 670 microsatellite and 158 AFLP markers tested for polymorphism, 341 (256 microsatellite and 85 AFLP markers) were used for genotyping with three F1 mapping panels, each comprising two parents and more than 100 progeny. Chi‐square goodness‐of‐fit test (χ2) revealed that only 19 microsatellite and 28 AFLP markers showed a highly significant segregation distortion (P < 0.005). Linkage analysis with a LOD score of 4.5 revealed 43 and 46 linkage groups in male and female linkage maps respectively. The male map consisted of 176 microsatellite and 49 AFLP markers spaced every ~11.2 cM, with an observed genome length of 2033.4 cM. The female map consisted of 171 microsatellite and 36 AFLP markers spaced every ~13.8 cM, with an observed genome length of 2182 cM. Both maps shared 136 microsatellite markers, and the alignment between them indicated 38 homologous pairs of linkage groups including the linkage group representing the sex chromosome. The karyotype of P. monodon is also presented. The tentative assignment of the 44 pairs of P. monodon haploid chromosomes showed the composition of forty metacentric, one submetacentric and three acrocentric chromosomes. Our maps provided a solid foundation for gene and QTL mapping in the tiger shrimp. 相似文献
17.
M. N. CORTINAS I. BARNES B. D. WINGFIELD M. J. WINGFIELD 《Molecular ecology resources》2006,6(3):780-783
Nine polymorphic microsatellite markers for the phytopathogenic fungus Colletogloeopsis zuluensis, the causal agent of an important stem canker disease of Eucalyptus, were isolated and characterized. Two methods, random amplified microsatellite sequences (RAMS) and fast isolation by AFLP of sequences containing repeats with modifications (M‐FIASCO), were used to isolate the microsatellites. Primers for 28 prospective microsatellite regions were designed and nine of these were polymorphic for C. zuluensis. Allelic diversity ranged from 0.12 to 0.80 with a total of 37 alleles. These markers will be used in future to determine the population genetic structure of C. zuluensis isolates and to monitor their global movement. 相似文献
18.
We developed eight PCR?primer pairs of polymorphic microsatellite loci for the túngara frog Physalaemus pustulosus. Genomic libraries were enriched for one of four microsatellite repeat sequences (CAn, GAn, ATGn and TAGAn). Following characterization of microsatellite loci by sequencing, primers were designed and PCR conditions optimized. Microsatellite PCR‐amplification was tested in 37 frogs from 8 populations in Costa Rica and Panama. Primer sequences, PCR conditions, allelelic diversities and observed as well as expected heterozygosities in the screened populations are described. 相似文献
19.
T. L. Maguire K. J. Edwards P. Saenger R. Henry 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(1-2):279-285
An enriched microsatellite library of the mangrove species Avicennia marina was constructed, in which 85.8% of the clones contained microsatellite sequences. Of the microsatellite repeat sequences
isolated, 55.0% were di-nucleotides, 34.2% were tri-nucleotides, 50.0% were perfect, 24.2% were imperfect, and 15.0% were
compound. Four different di-nucleotide repeats were isolated with repeat lengths ranging from 5 to 33; ten different tri-nucleotide
repeats were isolated with repeat lengths ranging from 3 to 25. The most common di-nucleotide was the AC/TG repeat; the most
common tri-nucleotide was the CCG/GGC repeat. Sixteen microsatellite sequences were selected for primer design, and 6 primers
were selected to investigate the polymorphism detected among 15 individuals of A. marina from three natural populations in Australia. A total of 40 alleles were detected at 6 microsatellite loci. The number of
alleles per microsatellite locus ranged from 5 to 13. On average, 7 alleles were detected per locus. All microsatellite loci
showed high levels of gene diversity (heterozygosity), with values ranging from 0.53 to 0.88; the mean value of gene diversity
was 0.70. Microsatellite loci were also tested for conservation across Avicennia species. There was a decline in amplification success with increasing divergence between Avicennia species. The results indicate that microsatellites are abundant in the Avicennia genome and can be valuable genetic markers for assessing the effects of deforestation and forest fragmentation in mangrove
communities, which is an important issue for mangrove conservation and afforestation schemes.
Received: 8 June 1999 / Accepted: 21 September 1999 相似文献
20.
Fragmentation and propagule formation are alternative reproductive strategies found in both plants and animals, with the latter generally providing greater dispersal capability. When both strategies occur, life history theory predicts that resources should be divided between the two. On coral reefs, both strategies are exhibited by branching corals and sponges, which are broken‐up after storm events and rapidly recolonize. In this study, we compared two congeneric Caribbean reef sponges, Callyspongia armigera, which is branched and easily fragmented, and C. vaginalis, which is not, to test whether there is a tradeoff in growth and propagule formation for C. armigera relative to C. vaginalis. Both species were equally abundant on coral reefs off Key Largo, Florida (10.1 ± 3.7 vs 11.9 ± 3.0 per 100 m2, respectively), suggesting that they are equally successful relative to two other non‐fragmenting congeneric species (C. fallax and C. plicifera) that are much less common. The number of substratum attachment points per sponge was significantly higher for C. armigera compared to C. vaginalis (2.31 ± 1.47 vs 1.03 ± 0.18 sponge?1), providing further evidence of the reliance of C. armigera on fragmentation, and of C. vaginalis on recruitment from larval settlement and subsequent growth. Growth rates in predator‐exclusion experiments were ~4‐fold higher for C. armigera compared to C. vaginalis (0.36 ± 0.31 vs 0.08 ± 0.11 % initial mass day?1), but C. armigera produced ~13‐fold fewer propagules than C. vaginalis (0.04 ± 0.22 vs 0.53 ± 1.08 % tissue area). Our results support a tradeoff between growth and propagule output for C. armigera relative to C. vaginalis, suggesting that these closely related sponge species took different evolutionary trajectories in reconciling their resource constraints. 相似文献