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1.
We isolated nine new microsatellites for the deep-sea fish Coryphaenoides rupestris. These loci are highly polymorphic, with allele number ranging from 14 to 42 and heterozygosity ranging from 0.647 to 0.965.
Four of these loci also amplified in C.
brevibarbis. 相似文献
2.
Eleven tetranucleotide microsatellite loci were developed for the Del Norte salamander (Plethodon elongatus). The loci were variably polymorphic, ranging from two to 20 alleles per locus, with expected heterozygosities ranging from 0.07 to 0.86. The loci also amplified in a congener, the Siskiyou Mountain salamander (P. stormi). The microsatellite loci will be used to assess the utility of highly polymorphic markers to assay within‐ and between‐species differentiation between these two closely related species. 相似文献
3.
G. Pellegrino D. Cafasso A. Widmer M. Soliva A. Musacchio S. Cozzolino 《Molecular ecology resources》2001,1(4):279-280
In this paper we describe the isolation and characterization of six polymorphic microsatellite loci from the orchid Serapias vomeracea. This species is widely distributed in the Mediterranean region. Microsatellite loci were isolated from an enriched library and primer pairs were designed for 18 loci. Primer pairs for six loci amplified well and were tested on samples from southern Italy. Levels of genetic variability detected at these six loci are high, with numbers of alleles per locus ranging from 3 to 6, and observed heterozygosity (HO) ranging from 0.35 to 0.86. All primer pairs tested amplified DNA from four other Serapias species, indicating that the primers are useful for population genetic studies throughout the genus. 相似文献
4.
M. Hughes M. Mller D. U. Bellstedt T. J. Edwards M. Woodhead 《Molecular ecology resources》2004,4(1):36-38
Microsatellite markers have been developed from standard enriched genomic libraries and a cDNA library for the genus Streptocarpus. Out of 15 loci derived from ESTs (expressed sequence tags), four gave working primer pairs, with expected heterozygosities (HE) ranging from 0.42 to 0.86. Out of 89 genomic library derived loci, 6 gave working primer pairs, with HE ranging from 0.63 to 0.93. 相似文献
5.
Microsatellite loci were isolated from Dawson's burrowing bee, Amegilla dawsoni, a species endemic to Western Australia. Twelve polymorphic loci were found with an observed number of alleles ranging from two to 24 and observed heterozygosities between 0.17 and 0.85. These 12 loci were tested for amplification in three additional species of Amegilla. The loci will be used for sex determination and the examination of mating frequency in this species. 相似文献
6.
Eight microsatellite loci were isolated and characterized from the Florida crown conch (Melongena spp.). Approximately 500 individuals from 20 populations encompassing all known Melongena spp. groups in Florida and Alabama were genotyped at all loci. High levels of variation were found with eight to 28 alleles per locus and observed per locus heterozygosity ranging from 0.148 to 0.779 (mean 0.624). One locus, Mco5, was monomorphic in more than half of the populations. These loci can provide useful information for a variety of studies ranging from parentage to population‐level analyses of the Florida crown conch. 相似文献
7.
Xin Deng Songhua Long Dongfeng He Xiang Li Yufu Wang Jia Liu Xinbo Chen 《Journal of plant research》2010,123(1):119-123
Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were
found to be polymorphic, with the number of alleles per locus ranging from two to six, and observed and expected heterozygosities
ranging from 0.125 to 0.375 (mean 0.013) and from 0.233 to 0.842 (mean 0.601), respectively. These polymorphic new microsatellite
loci will be useful for genetic linkage map construction, germplasm classification and identification, gene identification
and quantitative trait loci mapping, and marker-assisted selection in breeding in L. usitatissimum. 相似文献
8.
Wuchang bream (Megalobrama amblycephala) is an economically important fish in China. From a (GT)13‐enriched genomic library, 20 microsatellites were developed. Nine of these 20 loci were polymorphic in a test population with allele numbers ranging from two to four, and the observed and expected heterozygosities ranging from 0.2609 to 0.7826 and from 0.3739 to 0.7546, respectively. In the cross‐species amplifications, six of these nine loci were also polymorphic in white amur bream (Parabramis pekinensis). These polymorphic microsatellite loci are potentially useful for population genetics of Wuchang bream and its closely related species. 相似文献
9.
N. ALVAREZ C. BORN A.‐M. RISTERUCCI P. SOURROUILLE B. BENREY M. HOSSAERT‐MCKEY 《Molecular ecology resources》2004,4(4):683-685
Six microsatellite loci were isolated from the bruchid Acanthoscelides obtectus Say (Coleoptera: Bruchidae). Each locus was polymorphic, with the number of alleles ranging from 3 to 18. We found high levels of within‐population variation at most loci, with heterozygosities ranging from 0 to 0.75. Cross‐species amplification of these loci was tested in two other species of the genus Acanthoscelides, A. obvelatus Bridwell and A. argillaceus Sharp. 相似文献
10.
S. Aceto M. Sica G. Gamba S. Montieri A. Farina L. Gaudio 《Molecular ecology resources》2003,3(2):242-243
The isolation of molecular markers in Asparagus acutifolius, a wild edible plant species, is important to characterize local ecotypes that could be cultivated and preserved. We isolated and characterized polymorphic microsatellite loci from A. acutifolius by constructing and screening an enriched DNA library. Primer pairs were designed for 12 loci. Seven primer pairs worked well during amplification reactions and were tested on a wild population from Pontecagnano (SA), Italy. These loci showed a high level of genetic variability, with the numbers of alleles identified ranging from two to five and observed heterozygosity ranging from 0.20 to 0.73. 相似文献
11.
N. Alvarez A. Aebi A.‐M. Risterucci M. Hossaert‐Mckey B. Benrey 《Molecular ecology resources》2003,3(1):12-14
Five microsatellite loci were isolated from the bruchid Acanthoscelides obvelatus Bridwell (Coleoptera: Bruchidae). Each locus was polymorphic, with the number of alleles ranging from two to 15. We found high levels of within‐population variation at most loci, with heterozygosity ranging from 0.182 to 0.900. Cross‐species amplification of these loci was tested in two other species of the genus Acanthoscelides, A. obtectus Say and A. argillaceus Sharp. 相似文献
12.
JUDY ST. JOHN FINDLEY A. RANSLER THOMAS W. QUINN SARA J. OYLER‐MCCANCE 《Molecular ecology resources》2006,6(4):1083-1085
Primers for 16 microsatellite loci were developed for the trumpeter swan (Cygnus buccinator), a species recovering from a recent population bottleneck. In a screen of 158 individuals, the 16 loci were found to have levels of variability ranging from two to seven alleles. No loci were found to be linked, although two loci repeatedly revealed significant departures from Hardy–Weinberg equilibrium. Amplification in the closely related tundra swan (Cygnus columbianus) was successful for all except one locus. These microsatellite loci will be applicable for population genetic analyses and ultimately aid in management efforts. 相似文献
13.
SEEMANTI CHAKRABARTI SRINIVAS KAMBHAMPATI TONY GRACE LUDEK ZUREK 《Molecular ecology resources》2004,4(4):728-730
The house fly, Musca domestica L., is a cosmopolitan species with a capacity to transmit human pathogens. Here, we report on the development of polymorphic microsatellite loci for house flies and present preliminary results from four house fly subpopulations from Manhattan, Kansas. Twenty‐four microsatellite loci were isolated and characterized using DNA enriched for repeat sequences. Forty individuals from four locations in Kansas were assayed to identify for polymorphic loci. Eight loci were polymorphic with the number of alleles ranging from three to six. 相似文献
14.
C. H. NG S. C. KOH S. L. LEE K. K. S. NG A. MARK M. NORWATI R. WICKNESWARI 《Molecular ecology resources》2005,5(3):572-575
This study reports the isolation of 15 microsatellites in Acacia hybrid (Acacia mangium×Acacia auriculiformis) based on the 5′ anchored polymerase chain reaction technique. Polymorphism of these loci was evaluated in a sample of 24 hybrid individuals. The level of polymorphism ranged from two to eight alleles with an observed heterozygosity ranging from 0.083 to 0.875. These loci were also characterized in both the parental species. The number of alleles ranged from two to six for both A. mangium and A. auriculiformis with the observed heterozygosity values ranging from 0.167 to 0.625 and 0.042 to 0.458 respectively. Five of these loci demonstrated Mendelian inheritance in a segregating F1 population; four presented a distorted ratio and the remaining six did not segregate in progenies as they were homozygous in both parents. 相似文献
15.
Microsatellite loci were isolated from the Australian field cricket Teleogryllus oceanicus. Seven polymorphic loci were found with an observed number of alleles ranging from eight to 17 and observed heterozygosities between 0.26 and 0.94. One locus was found to be X‐linked. These seven loci were also tested for amplification and polymorphism in the congeneric species Teleogryllus commodus. The loci will be used for paternity studies in these species. 相似文献
16.
Song-Lin Chen Shi-Chao Xing Gen-Bo Xu Xiaolin Liao Jing-Feng Yang 《Conservation Genetics》2009,10(5):1469-1471
Small yellow croaker (Pseudosciaena polyactis) is an economically important marine fish species. About 43 microsatellite loci were isolated from two enriched genomic library
of Pseudosciaena polyactis. Ten of these loci were polymorphic in a test population with alleles per locus ranging from two to six, and observed and
expected heterozygosities per locus from 0.3750 to 0.8750 and from 0.3112 to 0.8121, respectively. No loci significantly deviated
from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs
of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of Pseudosciaena polyactis. 相似文献
17.
METTE SERINE WESMAJERVI TEKLE TAFESE J
RGEN STENVIK KJERSTI TURID FJALESTAD B
RGE DAMSGRD MADJID DELGHANDI 《Molecular ecology resources》2007,7(1):138-140
One hundred and fifty random clones from an enriched genomic library of Atlantic cod were sequenced. Primer pairs were designed for 15 microsatellites containing perfect di‐, tri‐, tetra‐ and hexanucleotide repeats and characterized in 96 unrelated fish. Eight markers were successfully amplified, with the number of alleles ranging from two to nine per locus and observed heterozygosity ranging from 0.341 to 0.977. Loci Gmo‐G13 and Gmo‐G14 had a significant excess of homozygotes. All loci conformed to the Hardy–Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed no significant departure from the null hypothesis between any of the loci. 相似文献
18.
Jing-Feng Yang Li-Li Zhao Chang-Wei Shao Xiang-Shan Ji Xiao-Lin Liao Song-Lin Chen 《Conservation Genetics》2009,10(5):1541-1543
Black snapper Sebastodes fuscescens (Houttuyn) is an economocally important species. Fourty-five microsatellite loci were isolated from an enriched genomic library
of S. fuscescens. Ten of these loci were polymorphic in a test population with alleles per locus ranging from 2 to 5, and observed and expected
heterozygosities per locus from 0.21 to 1.00 and from 0.19 to 0.86, respectively. Three loci significantly deviated from Hardy–Weinberg
equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs of loci. These polymorphic
microsatellite loci would be useful for investigating genetic diversity of S. fuscescens and other related species. 相似文献
19.
LEANNE WHEATON STEPHEN C. DONNELLAN MELITA C. DE VRIES MICHAEL G. GARDNER BRONWYN M. GILLANDERS 《Molecular ecology resources》2007,7(5):893-895
We isolated 10 polymorphic microsatellite loci for the giant Australian cuttlefish, Sepia apama, from a genomic library enriched for (AAC)n and (AAAG)n repetitive elements. In the nine loci that reliably amplified, the number of alleles ranged from four to 12 per locus with observed heterozygosity ranging from 0.343 to 0.926. These and a previously developed set of six loci will be useful for analysis of genetic structure of populations and determining input to a massive seasonal breeding aggregation in northern Spencer Gulf, Australia. 相似文献
20.
Tianjun Xu Changwei Shao Xiaolin Liao Xiangshan Ji Songlin Chen 《Conservation Genetics》2009,10(3):527-529
From a (GT)13-enriched genomic library of Oplegnathus fasciatus, 14 polymorphic microsatellite were isolated and characterized in a test population with alleles ranging from two to nine,
the observed and expected heterozygosities from 0.0000 to 1.0000, and from 0.1726 to 0.8507, respectively. Five loci deviated
from the Hardy–Weinberg equilibrium, and linkage disequilibrium between two loci was significant. Two loci were also polymorphic
in Pagrosomus major assessed for cross-species amplification. These polymorphic microsatellites will be useful for genetic diversity analysis
and molecule-assisted breeding for O. fasciatus.
Changwei Shao contributed equally. 相似文献