共查询到20条相似文献,搜索用时 31 毫秒
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PDGF induction of alpha 2 integrin gene expression is mediated by protein kinase C-zeta 总被引:3,自引:0,他引:3
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《The Journal of cell biology》1996,134(5):1301-1311
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Egr-1 mediates transcriptional repression of COL2A1 promoter activity by interleukin-1beta 总被引:2,自引:0,他引:2
Tan L Peng H Osaki M Choy BK Auron PE Sandell LJ Goldring MB 《The Journal of biological chemistry》2003,278(20):17688-17700
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The immediate-early gene product Egr-1 regulates the human interleukin-2 receptor beta-chain promoter through noncanonical Egr and Sp1 binding sites. 总被引:3,自引:0,他引:3
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The interleukin-2 IL-2 receptor beta-chain (IL-2Rbeta) is an essential component of the receptors for IL-2 and IL-15. Although IL-2Rbeta is constitutively expressed by lymphocytes, its expression can be further induced by a number of stimuli, including phorbol 12-myristate 13-acetate (PMA). We have now characterized factors that bind to an enhancer region located between nucleotides -170 and -139 of the human IL-2Rbeta promoter. Both Sp1 and Sp3 bound to the 5' portion of this region, whereas a PMA-inducible factor (PIF) mainly bound to its 3' portion and bound to the Sp binding motifs as well. In Jurkat T cells, induction of PIF DNA binding activity was rapidly induced, required de novo protein synthesis, and was sustained at a high level for at least 23 h. Interestingly, PIF was constitutively activated in human T-cell leukemia virus type 1-transformed MT-2 cells. In this paper, we demonstrate that PIF is Egr-1 based on its recognition by anti-Egr-1 antisera in gel mobility shift assays, even though the IL-2Rbeta DNA binding motif differed substantially from the canonical Egr-1 binding site. In addition, Egr-1 bound to the Sp binding site. In Jurkat cells, both sites were required for maximal IL-2Rbeta promoter activity, and in HeLaS3 cells, transfection of Egr-1 could drive activity of a reporter construct containing both sites. Moreover, Sp1 and Egr-1 could form a complex with kinetics that correlated with the production of Egr-1 in Jurkat cells upon PMA stimulation. Thus, Sp1 and Egr-1 physically and functionally cooperate to mediate maximal IL-2Rbeta promoter activity. 相似文献
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F L Day L A Rafty C N Chesterman L M Khachigian 《The Journal of biological chemistry》1999,274(34):23726-23733
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