Garcinìa dhanìkharíensis (Clusiaceae), a new species from Andaman Islands, India

A new species Garcinia dhanìkharíensis (Clusiaceae) from South Andaman, India is described and illustrated.     

Observation of a black‐cheeked waxbill (Brunhilda charmosyna) cleaning a Kirk’s dik‐dik (Madoqua kirkii)

The vast majority of interspecific interactions are competitive or exploitative. Yet, some positive interspecies interactions exist, where one (commensalism) or both (mutualism) species benefit. One such interaction is cleaning mutualisms, whereby a cleaner removes parasites from a client. In this note, we document the novel observation of a black‐cheeked waxbill (Brunhilda charmosyna) appearing to clean a Kirk''s dik‐dik (Madoqua kirkii), at the Mpala Research Centre in Laikipia County, Kenya. The purported cleaning took place for over one minute and is notable firstly for the dik‐dik remaining still for the duration of cleaning and secondly for involving two species that are much smaller than those traditionally involved in bird–mammal cleaning interactions. Unfortunately, no further cleaning events were subsequently observed, raising questions about whether this record was opportunistic or a regular occurrence. Future observations may reveal whether this behavior is widespread and whether it involves other small passerines.     

Familial whole-arm translocations (1;19), (9;13), and (12;21): a review of 101 constitutional exchanges

We report here on 3 familial whole-arm translocations (WATs), namely the 8th instance of t(1;19)(p10;q10) and 2 novel exchanges: t(9;13)(p10;q10) and t(12;21)(p10;q10). The exchanges (1;19) and (12;21) were ascertained through a balanced carrier, whereas the t(9;13) was first diagnosed in a boy with a trisomy 9p syndrome and der(9p13p). Results of FISH analyses with the appropriate α-satellite probes were as follows. Family 1, t(1;19): the D1Z5 probe gave a strong signal on both the normal chromosome 1 and the der(1q19p) as well as a weak signal on the der(1p19q). Family 2, t(9;13): the centromere-9 alphoid and D13Z1/D21Z1 probes under standard stringency gave no signal on the der(9p13p) in both the proband and a carrier brother, whereas the der(9q13q) was labelled only with the centromere-9 alphoid repeat in the latter; yet, this probe under low stringency revealed a residual amount of alphoid DNA on the der(9p13p) in the carrier. Family 3, t(12;21): the D12Z3 probe gave a signal on the normal chromosome 12 and the der(12p21q), whereas the D13Z1/D21Z1 repeat labelled the der(12q21p), the normal chromosome 21, and both chromosomes 13. Out of 101 WATs compiled here, 73 are distinct exchanges, including 32 instances between chromosomes with common alphoid repeats. Moreover, 7/9 of recurrent WATs involved chromosomes from the same alphoid family. Thus constitutional WATs appear to recur more frequently than other reciprocal exchanges, often involve chromosomes with common alphoid repeats, and can mostly be accounted for the great homology in alphoid DNA that favours mispairing and illegitimate nonhomologous recombination.     

Demography of a West African kob (Kobus kob kob) population

The kob (Kobus kob kob) population studied in the Comoé National Park, Ivory Coast, suffered unsustainable losses to poaching before and during the investigation. This in turn did not only lead to low densities but also changed the population composition, resulting in a strong female bias in all age classes except juveniles. The relative rarity of adult males probably had negative effects on the population recruitment. Although mating occurred throughout the year and birth intervals were short, at least in some females, annual birth‐rate was low compared to earlier studies in the same area. Effective conservation measures are urgently needed to ensure the future existence of the studied population.     

Sinai Tschulok (1875–1945)—a pioneer of Cladistics

Sinai Tschulok emigrated from the Ukraine to Switzerland, where he studied natural sciences, in particular biology. He founded and managed his own high school, which prepared students for entry to university-level education. This left him little time for research, which may explain why his work largely fell into oblivion. He did publish two influential books, however (Tschulok, S., 1910, Das System der Biologie in Forschung und Lehre, Gustav Fischer, Jena and Tschulok, S., 1922, Deszendenzlehre, Gustav Fischer, Jena), which were cited and commented upon favourably by both Walter Zimmermann and Willi Hennig. The most important point, in their opinion, was how Tschulok's explication of biological systematics had turned the "natural system" into a proof for the "Theory of Descent". The influence of Walter Zimmermann on Willi Hennig and the development of phylogenetic systematics is well known. Here some parts of Tschulok's writings are discussed that render him a pioneer in Cladistics.
© The Willi Hennig Society 2009.     

Paroniceratinae (Ammonoidea, Hildoceratidae) del Toarciense en las Cordilleras Ibérica y Cantábrica (España)

The Paroniceratinae constitute a group widely recorded in the Tethys realm. However, they represent alone a small part, less than 1%, of the ammonoidea obtained in Toarcian sediments from the regions located at the east of the Iberian Plateau (Iberian Range). The values are yet smaller in the northern regions (Cantabrian Range) and in the west (Portugal), and show a sporadic presence in the Betics Ranges. In this study, the biostratigraphical distribution and the biochronostratigraphical position of the recorded species in the more representative expanded sections of Spain are checked and compared with those of other areas of the NW Europe bioprovinces and of the Tethys. The obtained specimens from Frechiella: F. subcarinata (Young and Bird), F. helenae Renz, F. venantii (Catullo), F. kammerkarensis (Stolley) and F. cf. octaviae Renz are described and figured, all of them proceeding from sediments of the Bifrons Zone in the Iberian Range, as well as those of Paroniceras: P.sternale (d’Orbigny) and P.helveticum Renz, and Oxyparoniceras: O. (O.) cf. telemachi (Renz), O. (O.) buckmani (Bonarelli), O. (N.) cf. undulosum (Monestier), O. (N.) cf. morbiense (Renz) and O. (N.) cf. brocardi Rulleau, Bécaud and Neige, proceeding from sediments of the interval between the Bifrons Zone (Semipolitum Subzone) and the Insigne Zone (Insigne Subzone), in the Iberian and Cantabrian Ranges. The Frechiella and Oxyparoniceras species are developed and recorded in the Iberian Plateau margins during the Bifrons Zone and the Insigne Zone, respectively, during the same intervals than the transgressive maxima of the LJ3 and LJ4 second order cycles recognized in all the studied area, while Paroniceras species make it during the Variabilis Zone, that concur with the final regressive stage of the LJ3 cycle.     

Nondisjunction of a translocation-chromosome t(4;13)

Summary We have studied a male infant with multiple congenital anomalies due to a double derivative chromosome 13 resulting from an inherited balanced reciprocal translocation t(4;13)(q23;q12).     

Insect assemblages in a native (kanuka –

In New Zealand, the European shrub gorse (Ulex europaeus) is becoming the initial post-disturbance shrub, replacing the native myrtaceous manuka (Leptospermum scoparium) and kanuka (Kunzea ericoides) scrub in this role. Change in the dominant vegetation is likely to affect the native invertebrate community. To quantify these changes, we compared the assemblages of four selected insect taxa (Coleoptera, Lepidoptera, and two groups of Diptera, the Tachinidae and the fungus gnats, represented in New Zealand by the families Ditomyiidae, Keroplatidae and Mycetophilidae) in neighboring stands of kanuka and gorse using Malaise and pitfall traps set during December. We sorted 34 387 specimens into 564 recognisable taxonomic units. Ordinations showed that insect assemblages associated with each habitat were distinct for all four insect groups. The gorse habitat was species rich compared with kanuka for tachinids, fungus gnats and Malaise-trapped beetles, and both habitats contained few adventive species. Many species were unique to each habitat. Some species loss might occur if the kanuka-dominated community continues to be replaced by gorse, but gorse is nevertheless considered to be valuable as a habitat for native invertebrates.     

Forensic DNA confirms intraguild killing of a chuditch (<Emphasis Type="Italic">Dasyurus geoffroii</Emphasis>) by a feral cat (<Emphasis Type="Italic">Felis catus</Emphasis>)

Because animals killed by predators are often found partially consumed or decomposed, identification of the predator is often unachievable by post mortem examination. Forensic DNA offers an alternative in such situations. Using a novel method to analyse DNA from bite wounds on a freshly-killed chuditch Dasyurus geoffroii, we describe the first confirmed instance in this species of intraguild killing by a feral cat. Unlike post mortem examination, our method of DNA melt curve analysis is highly accurate and requires less time and expense than DNA sequencing.     

Feeding rhythmicity in a migratory copepod (Pseudodiaptomus hessei (Mrázek))

SUMMARY. This paper describes a day/night feeding rhythm in the copepod Pseudodiaptomus hessei in subtropical Lake Sibaya (27°20' S, 32°40' E) and examines the origin of this rhythmicity on the basis of field and laboratory observations.
Under natural conditions, adult and late copepodite stages (C_IV-C_V) of this copepod were predominantly benthic during daylight and migrated at dusk into pelagic waters in which they remained until dawn. Pronounced changes in feeding activity (based on the proportion of animals which contained food in the gut) were associated with this transition from a benthic to pelagic distribution; feeding activity was low by day and high at night. The younger copepodite stages (C_I-C_III) were less strongly benthic during daylight and showed correspondingly less pronounced diel changes in feeding activity.
Laboratory experiments involving concurrent light/dark and continuous dark regimes showed that batches of adult and C_IV—C_V stages fed throughout 24 h, although faecal pellet production increased with time under both light regimes. The laboratory observations are interpreted as negating the possibility that diel differences observed in the field arose through an endogenous feeding rhythm. The possibility that food resources in near-bottom waters was inadequate to support reasonable feeding activity is rejected on several counts. It is concluded that the feeding rhythm in P. hessei arises from an inferred infaunal distribution during daylight which would preclude filter-feeding activity. The circumstantial evidence supporting this suggestion is given and the suggestion is evaluated.     

In vitro synthesis of a microfibrillar (1→3)-β-glucan by a ryegrass (Lolium multiflorum) endosperm (1→3)-β-glucan synthase enriched by product entrapment

A simple, rapid procedure has been developed to purify (1→3)-β-glucan synthase (UDP-Glc:(1→3)-β-glucan 3-β-d -glucosyl transferase (EC 2.4.1.34)) over 400-fold from membrane preparations of Italian ryegrass (Lolium multiflorum) and 60-fold from CHAPS-extracted membranes. When a CHAPS-extract of the membranes is treated with 8 mM CaCl₂, proteinaceous material is precipitated. Although less than 10% of CHAPS-solubilized protein is removed in this step, the total activity recovered in the supernatant increases fourfold. Thus, CaCl₂ precipitation appears to be important in removing inhibitors of the (1→3)-β-glucan synthase. In the presence of 1 mM UDP-glucose, the supernatant after CaCl₂ treatment produces a high molecular weight, insoluble product that entraps a (1→3)-β-glucan synthase of high specific activity. The product-entrapped enzyme preparation contains six major polypeptides, and comparison of the SDS—PAGE pattern of this fraction with the polypeptide profile of an immunoprecipitated (1→3)-β-glucan synthase preparation suggests that polypeptides at 30–31 and 55–58 kDa are the most likely candidates for participation in (1→3)-β-glucan synthesis. When the reaction is performed on a larger scale, milligram quantities of product can be seen precipitating from the reaction mixture within 1 h of substrate addition. This product has been characterized by methylation analysis, ¹H- and ¹³C-nmr spectroscopy, X-ray diffraction, electron microscopy, size exclusion chromatography, UV-induced fluorescence in the presence of the (1→3)-β-glucan-specific fluorochrome from aniline blue, and enzymic hydrolysis with a specific (1→3)-β-glucanase. These physical, chemical and enzymic analyses clearly demonstrate that the product is a microfibrillar (1→3)-β-glucan with a degree of polymerization of about 1500.     

Pachytene analysis in males heterozygous for a familial translocation (9;12;13) (q22; q22; q32) ascertained through a child with partial trisomy 9

A family with four male and three female heterozygotes for a three-way translocation (9;12;13) (q22; q22; q32) in three generations was ascertained through a chromosomally imbalanced newborn with an additional derivative chromosome 9 resulting from nondisjunction. Three heterozygous males from two generations with apparent differences in their fertility status were investigated using pachytene spreads and testicular histology. Pachytene analysis in all three individuals, the fertile (II-2) as well as the subfertile (III-7) and infertile (III-9), showed a hexavalent with central nonpairing around the translocation breakpoints in nearly all spermatocytes. Thus, the observed hexavalent configurations in pachytene do not seem to have caused impaired fertility. This rather may have been the result of sperm carrying unbalanced chromosome sets. However, the observed difference in fertility between the heterozygous fertile male in generation II and his two heterozygous sons remains unexplained.     

Trisomy 6qter resulting from a familial (6;10) (q23;q26) translocation

An infant deceased at 2 months of age was found to have a 46,XY,-10, +der(10),t (6;10) (q23;q26) mat karyotype. Since the clinical findings were similar to those of the trisomy 6qter syndrome, the present observation agrees with the assignment of the 6q23----qter segment as the pathogenetic determiner of this entity.     

Inbreeding depression in <Emphasis Type="Italic">Solanum carolinense</Emphasis> (Solanaceae), a species with a plastic self-incompatibility response

Background  

Solanum carolinense (horsenettle) is a highly successful weed with a gametophytic self-incompatibility (SI) system. Previous studies reveal that the strength of SI in S. carolinense is a plastic trait, associated with particular S -alleles. The importance of this variation in self-fertility on the ability of horsenettle to found and establish new populations will depend, to a large extent, on the magnitude of inbreeding depression. We performed a series of greenhouse and field experiments to determine the magnitude of inbreeding depression in S. carolinense, whether inbreeding depression varies by family, and whether the estimates of inbreeding depression vary under field and greenhouse conditions. We performed a series of controlled self- and cross-pollinations on 16 genets collected from a large population in Pennsylvania to obtain progeny with different levels of inbreeding. We grew the selfed and outcrossed progeny in the greenhouse and under field conditions and recorded various measures of growth and reproductive output.