白细胞介素—2加强小鼠T淋巴细胞产生白细胞介素—3

In addition to the regulation of T cell growth, IL-2 exerts effects on the induction of certain lymphokines. We show here that IL-2 synergizes with 5 micrograms/ml of ConA to promote the production of IL-3 in mouse splenic T cell cultures. IL-3 was measured as CFU-GEMM-inducing activity on mouse bone marrow progenitor cells in the supernatant of the stimulated mouse splenic T cells (TCM). The resting T cells produced no CFU-GEMM-inducing activity, but could be induced to produce low level of CFU-GEMM-inducing activity in the presence of ConA. In vitro exposure to IL-2 markedly increased CFU-GEMM-inducing activity production (nearly up to 8-fold) by the ConA-activated T cells. Optimal stimulation was observed when 80 u/ml IL-2 was used for 48 h incubation. Anti-mouse IL-3 monoclonal antibody inhibited the CFU-GEMM inducing activity of TCM. Moreover, the TCM stimulated the proliferation of IL-3 dependent cell line FDC-P1. We also show that IL-2 and ConA-treated T cells expressed high level of IL-3 mRNA through dot blot analysis. These results confirmed the nature of CFU-GEMM-inducing activity of TCM as IL-3. The capacity of IL-2 to promote the production of IL-3 may represent an important mechanism by which it mediate the communication between the immune and hematopoietic systems.     

白细胞介素-2加强小鼠T淋巴细胞产生白细胞介素-3

本文报道了白细胞介素-2(IL-2)刺激ConA(5μg/ml)活化的小鼠T细胞产生的条件培养液(TCM)中含有CFU-GEMM诱导活性。这种CFU-GEMM诱导活性的生成在IL-2作用后48h达到高峰。特异性抗IL-3单克降抗体可以完全中和该条件培养液中的CFU-GEMM诱导活性。进一步证明,TCM可以刺激IL-3依赖细胞系FDC-P_1细胞的增殖;在IL-2作用于ConA活化的T细胞后可促进其细胞表达高水平的IL-3mRNA。这些结果表明IL-2可以加强小鼠T细胞产生IL-3。     

白细胞介素—2受体的研究进展

自Morgan等人于1976年发现了白细胞介素—2(Inter leukin—2 IL—2)以来,人们逐渐认识到IL—2是通过与其在T细胞表面上的受体结合后才产生生物学活性的.因而,揭开IL—2受体(IL—2receptor,IL—2R)的奥秘,对于了解IL—2与IL—2R相互作用方式及IL—2引导的信号传递机制,进而了解机体免疫应答与调控的原理,从而达到对某些免疫系统疾病预防、诊断和治疗的目的,具有重要意义.因此,近十多年来,人们投入了大量精力来研究IL—2R.本文将在回顾历史的基础上,重点介绍IL—2R领域研究的最新成果,以使读者对IL—2R有一个比较全面,系统的认识.     

白细胞介素—15及其受体

白细胞介素－１５是一种新发现的细胞因子,其生物学性质与ＩＬ－２相似,ＩＬ－１５的受体由ＩＬ－１５Ｒａ,ＩＬ－２Ｒβ,ＩＬ－２Ｒγ组成,其中ＩＬ－２Ｒγ是参与ＩＬ－１５信号传递过程的必要成分。ＩＬ－１５和ＩＬ－２的体内表达及受体分布存在差异预示着ＩＬ－１５在体内具有独特的生物学作用。     

前列腺素对肺泡巨噬细胞分泌白细胞介素—8的调控

本实验在经血清调理酵母聚糖激活的大鼠肺泡巨噬细胞（ＡＭ）体外培养４ｈ后,检测其上清液中的ＰＧｓ含量。消炎痛预处理使激活的ＡＭ释放的ＰＧＥ１、Ｅ２、Ｆ２ａ量显著减少,Ｅ２／Ｅ１和Ｅ２／Ｆ２ａ比值降低;而细胞松弛素Ｂ预处理ＡＭ则可使上述ＰＧｓ的含量增加。以中性粒细胞趋化指数来反映ＩＬ－８的活性,表明消炎痛预处理ＡＭ组的ＩＬ－８的分泌量显著减少,而细胞松弛素Ｂ预处理ＡＭ组的ＩＬ－８分泌量显著增多。ＡＭ的     

抗IBDV独特型抗体杂交瘤细胞株的建立及其产物生物学特性测定

用纯化的抗IBDV IgG免疫Balb/c小鼠,取其脾细胞与SP2/0细胞在聚乙二醇（PEG）作用下融合,ELISA法检测筛选,经有限稀释法克隆3次,获得2株（5F₄株,2B₆株）分泌抗IBDV独特型抗体的杂交瘤细胞株,其能诱生Balb/c小鼠产生ELISA抗体效价分别为1∶12 800和1∶25 600的含抗IBDV独特型抗体的腹水。用此独特型抗体与福氏完全佐剂和福氏不完全佐剂乳化制备成抗IBDV独特型抗体疫苗,免疫接种SPF鸡和普通京白公鸡,然后用IBDV强毒株(SD株)2000 ELD50攻毒,SPF鸡免疫组50只,有5只发病、2只死亡;对照组10只全部发病,8只死亡。普通京白鸡免疫组30只,有7只发病, 1只死亡;对照组10只全部发病,6只死亡。经X²检验,SPF鸡X²＝34.15,普通鸡X²＝16.68,查X²值表得X²_(1)0.01＝6.63, SPF鸡X²和普通鸡X2均大于X²_{(1) 0.01}（P＜0.01）,由此表明抗IBDV独特型抗体疫苗具有很好的免疫原性,对易感日龄的SPF鸡和普通鸡均具有极其明显的保护作用。从而证实了抗IBDV独特型抗体疫苗有潜在的研究和应用价值。     

甲型H1N1流感病毒血凝素蛋白单抗杂交瘤细胞株的制备与初步鉴定

目的:建立具有高特异、高效价的甲型H1N1流感病毒血凝素蛋白(HA)单抗的杂交瘤细胞株。方法:以纯化的昆虫杆状病毒表达的甲型H1N1流感病毒HA蛋白为免疫原免疫BALB/c小鼠,取脾细胞与Sp2/0小鼠骨髓瘤细胞融合,通过有限稀释法筛选阳性克隆,经ELISA和Western blot分析单抗的特性和特异性。结果:获得6株甲型H1N1流感HA抗原特异单克隆抗体杂交瘤细胞株,抗原肽库ELISA检测结果表明其中3株(1E12,3F12,1C11)单抗只与甲型H1N1流感HA抗原肽库反应,不与H5N1病毒HA抗原肽库反应;Western blot分析表明,单抗1B3只特异识别甲型H1N1流感HA抗原,而与其他季节性甲流病毒(H1,H3)及人禽流感H5N1病毒不反应。结论:所获杂交瘤细胞株特异性强,效价高,分泌抗体性能稳定,为分析甲型H1N1流感病毒抗原性位点、建立诊断试剂奠定了基础。     

Interleukin 4 drives phytohemagglutinin-activated T cells through several cell cycles: No synergism between interleukin 2 and interleukin 4

Cell kinetic studies of T cells stimulated with the interleukin 2 (Il-2), Il-4, or both lymphokines were performed with conventional [3H]thymidine incorporation and with the bivariate BrdU/Hoechst technique. Il-2 and Il-4 are able to drive phytohemagglutinin-activated T cells through more than one cell cycle. Neither synergistic nor inhibitory effect on T-cell proliferation was seen for the stimulation with both Il-2 and Il-4 as compared with the effect of Il-2 alone. The quantitative data of the cell cycle distribution of phytohemagglutinin-activated T cells suggest that the population of Il-4-responsive cells is at least an overlapping population, if not a real subset of the population of the Il-2-responsive cells.     

Interleukin 2 production in iron-deficient children

The relationship between iron status and capacity for IL-2 production by lymphocytes was assessed in 81 children from 6 mo to 3 yr of age selected at random from a population with low socioeconomic status, undergoing free systematic examination in four children's health centers in the Paris area. Iron deficiency was defined by the existence of at least two abnormal values among the three indicators of iron status: serum ferritin level ≤12 μg/L, transferrin saturation <12%, and erythrocyte protoporphyrin concentration >3 μg/g hemoglobin. According to this definition, 53 children were classified as iron deficient and 28 as iron sufficient. No differences were observed between the iron-deficient and iron-sufficient groups in terms of the IL-2 concentration without stimulation by PHA. IL-2 production by lymphocytes stimulated with PHA, as well as the stimulation index (ratio of IL-2 concentration following stimulation by PHA to that of IL-2 concentration without stimulation by PHA) were significantly lower in iron-deficient children. The reduction in IL-2 production by activated lymphocytes observed in our study of iron-deficient children may be responsible for impairments in immunity found by other authors, particularly in cell-mediated immunity.     

一种改进的分泌抗半抗原抗体杂交瘤细胞株筛选方法简

目的：研究解决半抗原分子单克隆抗体制备技术路径中遇到的在阳性杂交瘤细胞株筛选时无法排除载体蛋白问交叉反应影响的问题,以半抗原去甲肾上腺素（norepinephrine,NE）为例。方法：在NE完全抗原免疫小鼠实施细胞融合后,分别包被牛血清白蛋白（BSA）、卵清白蛋白（OVA）、BSA-NE、OVA-NE等4种不同抗原的酶标板平行检测细胞培养上清液;挑选BSA、OVA检测结果为阴性,BSA-NE、OVA-NE检测结果为阳性的孔内细胞进行克隆化筛选单克隆细胞。结果：本筛选方法可一次性从8板96孔板中筛选到13个符合要求的阳性孔,经3次克隆化后获得6株特异性强的杂交瘤细胞株。结论：本方法避免了载体蛋白间交叉反应对筛选的影响,改进了传统的单一指标筛选方法,筛选效率更高。     

白细胞介素2受体在人各种肿瘤细胞表面的表达

鉴于白细胞介素2受体(IL-2R)在某些恶性骨髓瘤、白血病及异常的T、B细胞和单核细胞表面异常高表达,近年来对IL-2与IL-2R结合肽P1-30的研究不断深入。简要综述了近年来国内外报道的IL-2R及其各组分在多种肿瘤细胞表面的分布情况,为以IL-2R或其不同组分为靶标的靶向治疗药物研究提供理论依据。     

白细胞介素18

白细胞介素-18(IL-18)是新发现的细胞因子,具有多种生物学功能.IL-18能促进外周血单个核细胞产生干扰素-γ(IFN-γ)、IL-2、粒细胞巨噬细胞集落刺激因子(GM-CSF)等细胞因子,增强天然杀伤细胞(NK细胞)的细胞毒作用.IL-18结构上与IL-1相似,而功能更接近IL-12,IL-18与IL-12均能诱导Th1细胞产生IFN-γ,存在协同效应,但它们的作用途径不同.IL-18在抗感染抗肿瘤等方面有着潜在的应用前景,并与自身免疫性疾病的发病密切相关.     

T cell exhaustion and Interleukin 2 downregulation

T cells reactive to tumor antigens and viral antigens lose their reactivity when exposed to the antigen-rich environment of a larger tumor bed or viral load. Such non-responsive T cells are termed exhausted. T cell exhaustion affects both CD8+ and CD4+ T cells. T cell exhaustion is attributed to the functional impairment of T cells to produce cytokines, of which the most important may be Interleukin 2 (IL2). IL2 performs functions critical for the elimination of cancer cells and virus infected cells. In one such function, IL2 promotes CD8+ T cell and natural killer (NK) cell cytolytic activities. Other functions include regulating naïve T cell differentiation into Th1 and Th2 subsets upon exposure to antigens. Thus, the signaling pathways contributing to T cell exhaustion could be linked to the signaling pathways contributing to IL2 loss. This review will discuss the process of T cell exhaustion and the signaling pathways that could be contributing to T cell exhaustion.     

白细胞介素13及其研究进展

白细胞介素13(IL-13)是最近被发现的一种新型细胞因子,由激活的辅助T细胞产生,其分泌产物主要是一种分子量约为10000的非糖基化的单链蛋白.人和鼠IL-13成熟蛋白分别由112和111个氨基酸残基组成,IL-13不仅对单核细胞的体外生长、表面抗原表达以及多种细胞因子的产生具有明显的调控作用,而且对B细胞的增殖、分化以及免疫球蛋白合成同样具有调控作用.更引人注目的是,在造血祖细胞的增植分化调控中,IL-13与其他造血生长因子有协同作用.     

Interleukin 2: from immunostimulation to immunoregulation and back again

Interleukin 2 (IL-2) was one of the first cytokines to be discovered. However, the complex role of IL-2 and its receptor in the regulation of immune responses is only now emerging. This review explores the various signals triggered by IL-2 and discusses their translation into biological function. A model is outlined that accommodates the seemingly contradictory functions of IL-2, and explains how one cytokine can be an essential T-cell growth and differentiation factor and yet also be indispensable to maintain peripheral tolerance.