Thermal ecology of two sympatric skinks (Mabuya macrorhyncha and Mabuya agilis] in a Brazilian restinga habitat

Abstract In a restinga habitat of southeastern Brazil, we studied some aspects of the thermal biology of two sympatric skinks (Mabuya macrorhyncha and Mabuya agilis) with distinct microhabitat preferences. The two species did not differ significantly in their mean body temperature. Sex and body size did not explain body temperature variation within either species. The body temperature of both species is significantly affected by air temperature, but only that of M. agilis is significantly influenced by sand surface temperature, presumably due to its more terrestrial habit compared to M. macrorhyncha. We found no significant seasonal variation in body temperature for either species, although environmental temperatures varied seasonally, indicating that both species may be selecting an optimal range of body temperatures, possibly through behavioural thermoregulation.     

A simple method to predict body temperature of small reptiles from environmental temperature

To study behavioral thermoregulation, it is useful to use thermal sensors and physical models to collect environmental temperatures that are used to predict organism body temperature. Many techniques involve expensive or numerous types of sensors (cast copper models, or temperature, humidity, radiation, and wind speed sensors) to collect the microhabitat data necessary to predict body temperatures. Expense and diversity of requisite sensors can limit sampling resolution and accessibility of these methods. We compare body temperature predictions of small lizards from iButtons, DS18B20 sensors, and simple copper models, in both laboratory and natural conditions. Our aim was to develop an inexpensive yet accurate method for body temperature prediction. Either method was applicable given appropriate parameterization of the heat transfer equation used. The simplest and cheapest method was DS18B20 sensors attached to a small recording computer. There was little if any deficit in precision or accuracy compared to other published methods. We show how the heat transfer equation can be parameterized, and it can also be used to predict body temperature from historically collected data, allowing strong comparisons between current and previous environmental temperatures using the most modern techniques. Our simple method uses very cheap sensors and loggers to extensively sample habitat temperature, improving our understanding of microhabitat structure and thermal variability with respect to small ectotherms. While our method was quite precise, we feel any potential loss in accuracy is offset by the increase in sample resolution, important as it is increasingly apparent that, particularly for small ectotherms, habitat thermal heterogeneity is the strongest influence on transient body temperature.     

Geographic variation in field body temperature of sceloporus lizards

1. Using data from the literature, I assessed how broad climatic patterns affected field body temperatures (T_b’s) of lizards in the genus Sceloporus.

2. Sceloporus at temperate latitudes had mean T_b’s of 35°C throughout their elevational range. This pattern is associated with “tropical” temperatures that extend into high north latitudes during the summer and the relatively low elevations occupied by the lizards.

3. At tropical latitudes, mean T_b declined from 35°C at low elevations to 31°C at high elevations. This pattern is associated with low seasonal variation in temperature at tropical latitudes and the relatively high elevations occupied by the lizards.     

Evolution of viviparity in warm-climate lizards: an experimental test of the maternal manipulation hypothesis

The maternal manipulation hypothesis for the evolution of reptilian viviparity has been claimed to apply to any situation where gravid females are able to maintain body temperatures different from those available in external nests, but empirical data that support this hypothesis are very limited. Here, we tested this hypothesis using gravid females of a warm-climate lizard, Mabuya multifasciata, by subjecting them to five thermal regimes for the whole gestation period. We found gravid females selected lower body temperatures and thermoregulated more precisely than did nongravid females. Offspring produced in different treatments differed in head size, limb length and sprint speed, but not in overall body size or mass. Variation in morphological traits of offspring was induced primarily by extreme temperatures. Sprint speed of offspring was more likely affected by the mean but not by the variance of gestation temperatures. Gravid females maintained more stable body temperatures than did nongravid females not because these temperatures resulted in the optimization of offspring phenotypes but because the range of temperatures optimal for embryonic development was relatively narrow. Our data conform to the main predictions from the maternal manipulation hypothesis that females should adjust thermoregulation during pregnancy to provide optimal thermal conditions for developing embryos and that phenotypic traits forged by maternal thermoregulation should enhance offspring fitness.     

Diet-induced obesity reduces core body temperature across the estrous cycle and pregnancy in the rat

Obesity during pregnancy causes adverse maternal and fetal health outcomes and programs offspring for adult-onset diseases, including cardiovascular disease. Obesity also disrupts core body temperature (T_c) regulation in nonpregnant rodents; however, it is unknown whether obesity alters normal maternal T_c adaptations to pregnancy. Since T_c is influenced by the circadian system, and both obesity and pregnancy alter circadian biology, it was hypothesized that obesity disrupts the normal rhythmic patterns of T_c before and during gestation. Obesity was induced by cafeteria (CAF) feeding in female Wistar rats for 8 weeks prior to and during gestation, whereas control (CON) animals had free access to chow. Intraperitoneal temperature loggers measured daily T_c profiles throughout the study, while maternal body composition and leptin levels were assessed near term. Daily temperature profiles were examined for rhythmic features (mesor, amplitude and acrophase) by cosine regression analysis. CAF animals exhibited increased fat mass (93%) and associated hyperleptinemia (3.2-fold increase) compared to CON animals. CAF consumption reduced the average T_c (by up to 0.29°C) across the estrous cycle and most of pregnancy; however, T_c for CAF and CON animals converged toward the end of gestation. Obesity reduced the amplitude of T_c rhythms at estrus and proestrus and on day 8 of pregnancy, but increased the amplitude at day 20 of pregnancy. Photoperiod analysis revealed that obesity reduced T_c exclusively in the light period during pre-pregnancy but only during the dark period in late gestation. In conclusion, obesity alters rhythmic T_c profiles and reduces the magnitude of the T_c decline late in rat gestation, which may have implications for maternal health and fetal development.     

Modelling photosynthetic responses to temperature of grapevine (Vitis vinifera cv. Semillon) leaves on vines grown in a hot climate

Field measurements of photosynthesis of Vitis vinifera cv. Semillon leaves in relation to a hot climate, and responses to photon flux densities (PFDs) and internal CO(2) concentrations (c(i) ) at leaf temperatures from 20 to 40 °C were undertaken. Average rates of photosynthesis measured in situ decreased with increasing temperature and were 60% inhibited at 45 °C compared with 25 °C. This reduction in photosynthesis was attributed to 15-30% stomatal closure. Light response curves at different temperatures revealed light-saturated photosynthesis optimal at 30 °C but also PFDs saturating photosynthesis increased from 550 to 1200 μmol (photons) m(-2)s(-1) as temperatures increased. Photosynthesis under saturating CO(2) concentrations was optimal at 36 °C while maximum rates of ribulose 1,5-bisphosphate (RuBP) carboxylation (V(cmax)) and potential maximum electron transport rates (J(max)) were also optimal at 39 and 36 °C, respectively. Furthermore, the high temperature-induced reduction in photosynthesis at ambient CO(2) was largely eliminated. The chloroplast CO(2) concentration at the transition from RuBP regeneration to RuBP carboxylation-limited assimilation increased steeply with an increase in leaf temperature. Semillon assimilation in situ was limited by RuBP regeneration below 30 °C and above limited by RuBP carboxylation, suggesting high temperatures are detrimental to carbon fixation in this species.     

Response of body temperature to solar radiation in restrained nymphal migratory grasshoppers (Orthoptera: Acrididae): influences of orientation and body size

Abstract. .We restrained forty-nine nymphal migratory grasshoppers (Melanoplus sanguinipes) in a series of orientations to the sun and measured rate of temperature change due to radiation intercepted, compensating for temperature loss by convection and greybody reradiation. Direct solar radiation affected body temperature significantly, but diffuse radiation did not. The coefficient of direct solar heating ( H _dir), varied from 0.603 to 0.690 depending on how radiation interception was estimated, and on whether insect size was expressed as mass or as a function of length. H is the rate of temperature change as a proportion of the maximum rate assuming complete conversion of all incident radiation to heat. None of the H_dir estimates was significantly different from visible-spectrum absorbance to (0.722), suggesting that the latter is a reasonable approximation of H _dir
In simulations, equilibrium body temperature elevation decreased with body size where energy influx was expressed as the amount intercepted (W) , but increased with body size when expressed as insolation as (W m-²) because at a specified insolation, the amount of energy intercepted increases with body size. Expressing energy influx as the amount intercepted reveals the underlying biophysical mechanisms which determine body temperature.
This paper presents a model of body temperature elevation in which fundamental physical processes are assembled to provide good estimates. This basis in physics implies that the model can can be applied with confidence to conditions other than those in which it was derived.     

Adjustment of vibratory signals to ambient temperature in a host-searching parasitoid

Abstract.  Certain ichneumonid parasitoids (Hymenoptera) use self-produced vibrations transmitted on plant substrate, so-called vibrational sounding, to locate their immobile concealed pupal hosts. An ambient temperature dependency with higher frequencies and intensities at higher temperatures is postulated because signals are of myogenic origin. Here, temperature influence on vibratory signals is analysed in the temperate parasitoid Pimpla turionellae under different thermal conditions using plant-stem models to elicit host-searching behaviour. Signals are measured with laser Doppler vibrometry and analysed for time parameters and frequency components applying fast-Fourier transformations. The results reveal an unexpected effect of ambient temperature on signals produced by the female wasps. Although average values of time parameters (pulse trains, pulse train periods, inter pulse duration) are unchanged by ambient temperature, the frequency parameters show an inverse thermal effect. Within the temperature range tested (8–26 °C), decreasing temperature leads to significantly higher frequency and intensity of the self-produced vibrations in the temperate species. This inverse thermal effect may be explained by a temperature-coupled signal production in the frequency domain to compensate negative low-temperature effects on the mechanoreceptors by increased muscle activity. The option of heterothermy to produce signals reliably during vibrational sounding under low temperature is also discussed.     

Highly‐enhanced larval growth during the cold season mediated by the basking behavior of the butterfly Parnassius citrinarius (Lepidoptera: Papilionidae)

Some insect species are thought to grow quickly, even in low temperatures under natural conditions, presumably by conducting basking behaviors to use sunlight. However, whether basking behavior in fact enhances developmental speed and shortens the larval period in the field has not been determined. Moreover, few studies have examined whether basking is behavioral thermoregulation or simply the result of highly‐heterogeneous heat environments in the field. To examine these issues, we conducted field observations and laboratory experiments using larvae of Parnassius citrinarius Motschulsky, which mature within a short period after the thaw in early spring. First, body temperatures of larvae were measured under sunny and cloudy conditions. Second, larval preference for warmer locations was examined. Finally, we compared the developmental speed of larvae when they basked under field conditions and when did not bask in laboratory conditions under different air temperature regimes. Under sunny conditions, larval body temperature was substantially higher than either the temperature of the host plant or the air temperature, and was equivalent to the temperature of dead leaves, which the larvae used as basking sites. In contrast, no such tendency was observed under cloudy conditions. Larvae exhibited an exclusive preference for warmer locations. Moreover, in the field, despite the low ambient temperature, larvae grew much faster than those reared in the laboratory. These results imply that the basking behavior of P. citrinarius larvae is active thermoregulation to maintain high body temperatures in the cold season.     

Effects of phenotypic variation onto body temperature and flight activity in a polymorphic insect

Abstract.  According to biophysical principles, colour and size are important phenotypic factors that may influence body temperature and activity in ectothermic insects. In taxa showing female-limited polymorphism, males and female morphs differ in body colour, size and activity pattern. However, no previous study has evaluated whether such phenotypic and behavioural variation relates to differences between males and female morphs in thermal properties. In the present study, the relationships between body colour, size, activity and body temperature are examined under laboratory and field conditions, for the polymorphic damselfly Enallagma cyathigerum (Charpentier, 1840) (Odonata: Zygoptera). Contrary to expectation, males and female colour morphs of this species do not differ in thermal properties (i.e. heating characteristics or field body temperatures). When questioning phenotype and activity, temperature does not appear to be relevant for understanding the maintenance of female-limited polymorphism.     

Modelling the establishment and spread of autotetraploid plants in a spatially heterogeneous environment

The establishment and spread of autotetraploids from an original diploid population in a heterogeneous environment were studied using a stochastic simulation model. Specifically, we investigated the effects of heterogeneous habitats and nonrandom pollen/seed dispersal on the critical value (micro) of unreduced 2n gamete production necessary for the establishment of autotetraploids as predicted by deterministic models. Introduction of a heterogeneous environment with random pollen/seed dispersal had little effect on the micro value. In contrast, incorporating nonrandom pollen/seed dispersal into a homogeneous environment considerably reduced the micro value. Incorporating both heterogeneous habitats and nonrandom pollen/seed dispersal may lead either to an increase or to a decrease in the micro value compared to that with random dispersal, indicating that the two factors interact in a complex way.     

Effects of a five-hour exposure of arm and leg to a slightly cool thermal environment in the afternoon on the body temperature rhythm

The purpose of this study was to determine whether the different thermal conditions of the arm and leg due to wearing different types of clothing during the afternoon could modulate the circadian rhythm of body temperature and subjective sleep quality. Six healthy female volunteers were studied twice with two types of clothing, leaving the arm and leg covered or uncovered. The environmental chamber was controlled at 24 ± 0.5°C and 50 ± 5% RH during wakefulness and 28 ± 0.5°C and 50 ± 5% RH during night sleep. One type of clothing consisted of long-sleeved shirts and full-length trousers (Type L, 989 g, 0.991 clo); the other type was of half-sleeved shirts and knee-length trousers (Type H, 750 g, 0.747 clo). One testing session lasted for 16 h from 14:00 to 06:00 h the next morning. Subjects wore Type L or Type H clothing during the afternoon exposure (14:00 - 19:00), and Type L clothing during the evening (19:00 - 22:30 h) and the night sleep (22:30 - 06:00 h). Results were as follows. (1) When wearing Type H rather than Type L clothing, skin temperatures of the arm and leg were significantly lower during the time of exposure, and increased more after the evening. (2) Rectal temperature was not significantly different between the two types of clothing except during the early part of the exposure period, but it decreased during the evening by a significantly greater amount when wearing Type H clothing. (3) Subjective sleep quality was not significantly different between the two clothing types. These results suggest that afternoon exposure of the arm and leg to a slightly cool environment does not have a strong after-effect on the body temperature and subjective sleep quality.     

Physiological colour change in the Moorish gecko,Tarentola mauritanica (Squamata: Gekkonidae): effects of background,light, and temperature

Colour has many different functions in animals, such as an involvement in thermoregulation, crypsis, and social interactions. Species capable of physiological colour change may alter their coloration in response to ecological conditions. The Moorish gecko, Tarentola mauritanica, is capable of actively changing its body coloration. In the present study, we investigated colour change in this gecko as a function of background, temperature, and light. Our results demonstrate that the Moorish gecko indeed changes its dorsal colour in response to changes in environmental conditions. By contrast to several other reptilian species, this rapid colour change does not appear to be associated with thermoregulation. Background matching, however, did appear to be a prominent function, although illumination appears to be an essential trigger. Future research should concentrate on individual variation and its effectiveness with respect to antipredatory mechanisms. © 2012 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, ?? , ??–??.     

Modelling and predicting the effect of temperature,water activity and pH on growth of Streptococcus iniae in Tilapia

Aims: To evaluate and model the growth of Streptococcus iniae affect by temperatures (10–45°C), water activity (A_w; 0·995–0·957), and pH (5–8). Methods and Results: Temperatures, A_w, and pH were adjusted. The behaviour of S. iniae was studied and modelled. Growth curves were fitted by using logistic, Gompertz, and Baranyi models. The maximum growth rates obtained from the primary model were then modelled as a function of temperature, A_w, and pH using the Belehradek‐type models for secondary model. The optimum values for growth were found to be in the range of 35–40°C, A_w 0.995–1, and pH 6–7. The statistical characteristics of the models were validated by r², mean square error, bias, and accuracy factors. The results of validation indicated that Baranyi model performed the best. Conclusions: The effect of temperature, A_w/NaCl, pH control of S. iniae in tilapia could be satisfactorily predicted under current experimental conditions, and the proposed models could serve as a tool for this purpose. Significance and Impact of the Study: The suggested predictive model can be used for risk assessment concerning S. iniae in tilapia.