共查询到20条相似文献,搜索用时 15 毫秒
1.
Qingsheng Zhao Bingxian XieJun Yan Fangchun Zhao Jie Xiao Lingyun Yao Bing Zhao Yunxiang Huang 《Carbohydrate polymers》2012,87(1):392-396
Ultrasonic circulating extraction technology was applied for the polysaccharide extraction from Asparagus officinalis. The crude polysaccharides were deproteinized by Sevag method and three main polysaccharide fractions, AOP-4, AOP-6 and AOP-8 were obtained by fractional precipitation with gradient concentrations of ethanol (40%, 60% and 80%). The in vitro antitumor and antioxidant activities of the polysaccharide fractions were evaluated by MTT assay and free radical-scavenging assay, respectively. Deproteinized AOPs showed higher antioxidant and antitumor activities than crude AOP. AOP-4 with molecular weight 5.75 × 104 Da showed significant function of scavenging hydroxyl radical. Three AOP fractions had significant antitumor activity against HeLa and BEL-7404 cells in a dose dependent manner. Furthermore, the inhibit activity of AOP-4 against HeLa cells was higher than those of other AOPs and the inhibition rate reached 83.96% at the concentration of 10 mg/mL. These results indicated that the AOP might be useful for developing natural safe antitumor drugs or health food. 相似文献
2.
Haibin Tong Ximing Liu Dan Tian Xin Sun 《World journal of microbiology & biotechnology》2013,29(5):775-780
Tricholoma matsutake, a high-class edible mushroom in China, has been regarded as famous foods and biopharmaceutical materials with a great deal of interest. In the previous investigations, researchers believe the water-soluble polysaccharide β-glucan is the major active component of T. matsutake, which displays various biological activities. In the present study, two novel alkali-extracted polysaccharide fractions, TM-APS-1 and TM-APS-2, were isolated from the fruit bodies of T. matsutake by DEAE-cellulose and Sepharose CL-6B columns on ÄTKA explorer chromatography system. Their chemical and physical characteristics and radical scavenging capacity were valuated, including chemical methods, GC, HPLC, scavenging activity against DPPH radicals, superoxide radicals, hydroxyl radicals, and chelating ability. The results showed that TM-APS-1 and TM-APS-2 exhibited significantly antioxidant activity at a concentration-dependent manner. The alkali-extracted polysaccharide fractions from T. matsutake can be developed to be novel functional food or pharmaceutical products with antioxidant effects. 相似文献
3.
Zhang Z Wang X Yu S Yin L Zhao M Han Z 《International journal of biological macromolecules》2011,49(5):1012-1015
Sulfated polysaccharide extracted from blue algae Enteromorpha linza is proved to possess excellent antioxidant activities. Two derivatives by means of oversulfated and acetylated coupling were synthesized and investigated their antioxidant activities including scavenging effect of superoxide, hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals. And then the relationship between antioxidant activity and chemical characteristics was characterized. The results of chemical analysis and FT-IR showed that the modification of polysaccharide was successful. In addition, it was found that certain derivatives exhibited stronger antioxidant activity than raw material. They could serve as free-radical inhibitors or scavengers, acting possibly as primary antioxidants. 相似文献
4.
In vitro antioxidant activities of sulfated polysaccharide fractions extracted from Corallina officinalis 总被引:1,自引:0,他引:1
Yang Y Liu D Wu J Chen Y Wang S 《International journal of biological macromolecules》2011,49(5):1031-1037
Sulfated polysaccharides (F1, F2) from seaweed Corallina officinalis were isolated through anion-exchange column chromatography. Their chemical characteristics were determined by GC, HPLC, FT-IR and UV spectra. F1 and F2 contained only two monosaccharides, namely galactose and xylose. The antioxidant activities of F1, F2 and the de-sulfated polysaccharides (DF-1, DF-2) in vitro were investigated, including hydroxyl radicals scavenging effect, superoxide radical scavenging capacity, DPPH radical activity and reducing power. As expected, antioxidant assay showed that the two sulfated polysaccharide fractions (F1, F2) possessed considerable antioxidant properties and had more excellent abilities than de-sulfated polysaccharides (DF-1, DF-2). 相似文献
5.
Yin ChenWenjun Mao Yupin YangXiancun Teng Weiming ZhuXiaohui Qi Yanli ChenChunqi Zhao Yujiao HouChunyan Wang Na Li 《Carbohydrate polymers》2012,87(1):218-226
An extracellular polysaccharide AVP was isolated from the fermented broth of coral-associated fungus Aspergillus versicolor LCJ-5-4. AVP was a mannoglucan with molecular weight of about 7 kDa, and the molar ratio of glucose and mannose was 1.7:1.0. On the basis of detailed one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR) spectroscopic analyses, the backbone of AVP was characterized to be composed of (1 → 6)-linked α-d-glucopyranose and (1 → 2)-linked α-d-mannopyranose units. The mannopyranose residues in the backbone were substituted mainly at C-6 by the side chain of (1 → 2)-linked α-d-mannopyranose trisaccharides units. The antioxidant activity of AVP was evaluated with the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals in vitro, and the results indicated that AVP had good antioxidant activity, especially scavenging ability on superoxide radicals. AVP was a novel extracellular polysaccharide with different structural characteristics from other extracellular polysaccharides and could be a potential source of antioxidant. 相似文献
6.
Fucose-containing sulfated polysaccharides from brown macroalgae Lobophora variegata with antioxidant,anti-inflammatory,and antitumoral effects 总被引:1,自引:0,他引:1
Luiza Sheyla E. P. Will Castro Thuane S. Pinheiro Allisson J. G. Castro Celina M. P. G. Dore Naissandra B. da Silva Monique Gabriela das C. Faustino Alves Marilia S. Nascimento Santos Edda Lisboa Leite 《Journal of applied phycology》2014,26(4):1783-1790
This study analyzed sulfated polysaccharides, such as fucans, from the brown alga Lobophora variegata to verify their antioxidant activity in vitro, antitumoral effect on human colon adenocarcinoma cell line HT-29, and anti-inflammatory activity. Sulfated polysaccharide fractions containing fucans were obtained after fractionation with increasing volumes (v) of acetone (0.3–2.0 v). The polysaccharide was eluted with 1.5 v of acetone and named F1.5. The results showed that F1.5 contained a high yield. Chemical and structure analyses were performed by infrared spectroscopy, electrophoresis in agarose gel, and chemical dosages (sugar, protein, phenolic compounds, and sulfate). We observed that this sulfated polysaccharide had antioxidant activity and antitumoral effect. Anti-inflammatory activity in vivo of F1.5 was observed in the croton oil mouse-ear model at 75 mg kg-1. The results were correlated with histopathological analysis. 相似文献
7.
Purification and biochemical characterization of antioxidant peptide from horse mackerel (Magalaspis cordyla) viscera protein 总被引:2,自引:0,他引:2
In the present study, a peptide having high antioxidant properties was isolated from horse mackerel viscera protein, Magalaspis cordyla. In vitro gastrointestinal digestion was employed to obtain potential protein hydrolysate and was subjected to consecutive chromatographic methods using fast protein liquid chromatography (FPLC) connected to diethyl amino ethyl (DEAE) anion exchange column and Sephadex G-25 gel filtration column. The activity of the fractions was tested against DPPH and hydroxyl radicals and the isolated peptide showed 89.2 and 59.1 percentage of scavenging. The amino acid sequence of purified peptide was determined using ESI-MS/MS as Ala-Cys-Phe-Leu (518.5 Da), it exhibited high activity against polyunsaturated fatty acid (PUFA) peroxidation than that of natural antioxidant, α-tocopherol. 相似文献
8.
He L Ji P Gong X Li W Cheng J Qian H Song X 《International journal of biological macromolecules》2011,49(3):422-427
A novel water-soluble polysaccharide pMTPS-3, obtained from Melia toosendan Sieb. Et Zucc fruit by hot-water extraction and ethanol precipitation, was fractionated by DEAE-52 cellulose anion-exchange and Sephadex G-100 gel filtration chromatography. Its primary structural features and molecular weight were characterized by Fourier infrared spectrometry (FTIR), gel permeation chromatography (GPC) and gas chromatography (GC). And the antioxidant activities of pMTPS-3 in vitro were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, superoxide radical scavenging assay and hydroxyl radical scavenging assay. The results suggested that pMTPS-3 was a heteropolysaccharide, composed of arabinose, glucose, mannose, and galactose in the molar ratio of 17.3:28.3:41.6:12.6 with molecular weight 26 100 Da. The purified pMTPS-3 was revealed to have notable scavenging activity against DPPH radical in a concentration-dependent manner and present a moderate inhibition of superoxide radicals with an IC50 (5.6 mg/ml), and potent inhibiting power for hydroxyl radical compared with crude polysaccharide. Further, it exhibited strong inhibition effect in vitro on the growth of human gastric cancer BGC-823 cells. It is strongly evidenced that pMTPS-3 purified from the crude polysaccharides of Melia toosendan Sieb. Et Zucc could be explored as a potential antioxidant and therapeutics. 相似文献
9.
Background
Based on the ethnomedicinal uses and the effective outcomes of natural products in various diseases, this study was designed to evaluate Isodon rugosus as possible remedy in oxidative stress, alzheimer’s and other neurodegenerative diseases. Acetylecholinestrase (AChE) and butyrylcholinesterase (BChE) inhibitory activities of crude methanolic extract (Ir.Cr), resultant fractions (n-hexane (Ir.Hex), chloroform (Ir.Cf), ethyl acetate (Ir.EtAc), aqueous (Ir.Aq)), flavonoids (Ir.Flv) and crude saponins (Ir.Sp) of I. rugosus were investigated using Ellman’s spectrophotometric method. Antioxidant potential of I. rugosus was determined using DPPH, H2O2 and ABTS free radicals scavenging assays. Total phenolic and flavonoids contents of plant extracts were determined and expressed in mg GAE/g dry weight and mg RTE/g of dry sample respectively.Results
Among different fractions Ir.Flv and Ir.Cf exhibited highest inhibitory activity against AChE (87.44 ± 0.51, 83.73 ± 0.64%) and BChE (82.53 ± 0.71, 88.55 ± 0.77%) enzymes at 1 mg/ml with IC50 values of 45, 50 for AChE and 40, 70 μg/ml for BChE respectively. Activity of these fractions were comparable to galanthamine causing 96.00 ± 0.30 and 88.61 ± 0.43% inhibition of AChE and BChE at 1 mg/ml concentration with IC50 values of 20 and 47 μg/ml respectively. In antioxidant assays, Ir.Flv, Ir.Cf, and Ir.EtAc demonstrated highest radicals scavenging activities in DPPH and H2O2 assays which were comparable to ascorbic acid. Ir.Flv was found most potent with IC50 of 19 and 24 μg/ml against DPPH and H2O2 radicals respectively. Whereas antioxidant activates of plant samples against ABTS free radicals was moderate. Ir.Cf, Ir.EtAc and Ir.Cr showed high phenolic and flavonoid contents and concentrations of these compounds in different fractions correlated well to their antioxidant and anticholinestrase activities.Conclusion
It may be inferred from the current investigations that the Ir.Sp, Ir.Flv and various fractions of I. rugosus are good sources of anticholinesterase and antioxidant compounds. Different fractions can be subjected to activity guided isolation of bioactive compounds effective in neurological disorders. 相似文献10.
Extraction, preliminary structural characterization, and antioxidant activities of polysaccharides from Salvia miltiorrhiza Bunge 总被引:1,自引:0,他引:1
Four polysaccharides were extracted from Salvia miltiorrhiza Bunge using hot water, ultrasonic, alkali, and enzyme methods. Preliminary structural characterization was conducted by physicochemical property, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) analyses. Antioxidant activities against 2,2-diphenyl-1-picryl-hydrazyl (DPPH), hydroxyl, and superoxide radicals were also evaluated. The physicochemical property analysis indicated the identicalness of the polysaccharide indices obtained by the hot water and ultrasonic methods. The indices obtained by the alkali and enzyme methods were significantly different. The FTIR spectra revealed the general characteristic absorption peaks of the four polysaccharides. The SEM images demonstrated significant differences in the surface features of the different polysaccharides. The antioxidant activity assay revealed the significant antioxidant activities of three polysaccharides. Overall, the polysaccharides from S. miltiorrhiza Bunge may have potential applications in the medical and food industries. 相似文献
11.
Antioxidant activity of a water-soluble polysaccharide purified from Pteridium aquilinum 总被引:1,自引:0,他引:1
A water-soluble crude polysaccharide, obtained from fern Pteridium aquilinum, was fractionated by DEAE-Sepharose Fast-Flow column chromatography, and purified by Sephacryl S-400 HR column chromatography. The average molecular weight (Mw) of the purified polysaccharide (PLP) is 458,000 Da. The monosaccharide components of PLP were characterized by gas chromatography (GC), and the majority of the monosaccharide components was glucose (relative mass 58.1%) with low levels of galactose, mannose, rhamnose, and arabinose (relative mass 18.7%, 6.8%, 10.2%, and 6.1%, respectively). The Fourier-transform infrared spectra (FTIR) of PLP revealed typical characteristics of polysaccharides. On the basis of the ferric-reducing antioxidant power assay (FRAP), DPPH radical-scavenging, the superoxide radical assay, and self-oxidation of 1,2,3-phentriol assay, the antioxidant activities of PLP were investigated. The purified polysaccharide was demonstrated to have strong reductive power (FRAP value: 827.6 μmol/L), moderate scavenging activities against DPPH radicals (83.1%) and superoxide radicals (60.5%), and moderate inhibiting power for self-oxidation of 1,2,3-phentriol (52.4%). 相似文献
12.
C.L. Lin C.C. Wang S.C. Chang B. Stephen Inbaraj B.H. Chen 《International journal of biological macromolecules》2009,45(2):146-151
Antioxidant activity of polysaccharide fractions isolated from Lycium barbarum Linnaeus was evaluated. Polysaccharides were extracted with boiling water, followed by precipitating with ethanol, protein hydrolysis, dialysis, and fractionation with a DEAE–Sepharose CL-6B column. A total of 4 fractions, including 1 neutral polysaccharide (LBPN) and 3 acidic polysaccharides were obtained, and compared with crude polysaccharide (CP), crude extract of polysaccharide (CE), deproteinated polysaccharide (DP), and deproteinated and dialyzed polysaccharide (DDP) for antioxidative activity. With the exception of CE and DDP, most polysaccharides were effective in scavenging DPPH and ABTS+ free radicals, superoxide anion and hydroxyl radical at 1000 μg/mL. 相似文献
13.
The composition, molecular weight and in vitro antioxidant activity of various sulfated polysaccharides obtained by anion
exchange chromatography, acid hydrolysis and radical process degradation of the crude sulfated polysaccharide extracted from
Laminaria japonica were compared. The low sulfated F-A2, with a peak-molecular weight (Mp) of 5–15 kDa, 14.5% sulfated ester and 21.8% glucuronic
acid, exhibited a very strong antioxidant activity on superoxide and hydroxyl radicals, with activity even higher than that
of large molecular weight fractions F-A and F-B. However, highly sulfated fractions with a peak-molecular weight below 15 kDa
had much lower antioxidant activities than other fractions. These results indicated that the sulfate group of the low molecular
weight fractions represents a physical block for the reaction with oxygen radicals. The chemical properties and antioxidant
activities of sulfated polysaccharide fractions obtained by radical process degradation of crude sulfated polysaccharide were
quite different from those obtained by acid hydrolysates. By radical process degradation, the high molecular weight was decreased
to give LM2 (Mp 8 kDa) and LM1 (Mp 1.5 kDa), with a yield of 40% and 15%, respectively. LM2 was enriched with fucose and sulfated
ester, while containing low amounts of glucuronic acid. The antioxidant activity showed that LM2 was unable to scavenge either
superoxide or hydroxyl radical, which suggested that radical process degradation targeted mainly ascopyllan-like species rich
in glucuronic acid, while the fraction rich in sulfated l-fucose remained unchanged. However, LM1 with Mp 1.5 kDa still retained apparent scavenging ability for superoxide radical,
although it contained no glucuronic acid and certain amounts of galactose and mannose as main neutral sugars. These result
suggest that the antioxidant activity of sulfated polysaccharides is apparently related not only to molecular weight and sulfated
ester content, as previously determined, but also to glucuronic acid and fucose content. 相似文献
14.
In this study, three alginate fractions with different molecular weights and ratios of mannuronic acid (M) to guluronic acid
(G) were prepared by enzymatic hydrolysis and ultrafiltration to assess the antioxidant property of alginates from Laminaria japonica with molecular weight below 10 kDa. The antioxidant properties of different molecular weight alginates were evaluated by
determining the scavenging abilities on superoxide, hydroxyl, and hypochlorous acid and inhibitory effect on Fe2+-induced lipid peroxidation in yolk homogenate. The results showed that low molecular weight alginates exhibited high scavenging
capacities on superoxide, hydroxyl, and hypochlorous acid radicals and good inhibition of Fe2+-induced lipid peroxidation in yolk. By comparison, alginate A1 with molecular weight below 1 kDa and M/G of 1.84 had better
scavenging activity on superoxide, hydroxyl, and hypochlorous acid radicals in vitro than A2 (1–6 kDa), A3 (6–10 kDa), ascorbic
acid, and carnosine. With similar M/G ratio, A2 exhibited better antioxidant activity on superoxide and hypochlorous acid
radicals than A3. However, fraction A3 with molecular weight of 6–10 kDa exhibited higher inhibitory ability on lipid peroxidation
in yolk in vitro than A1 and A2. The results indicated that molecular weight played a more important role than M/G ratio on
alginate to determine the antioxidant ability. By comparison, low molecular weight alginates composed of guluronic acid and
mannuronic acid exhibited better antioxidant ability on oxygen free radicals than sulfated polysaccharides from L. japonica in our previous study and represent a good source of marine polysaccharide with potential application as natural antioxidant. 相似文献
15.
The cells of the red microalga Porphyridium UTEX 637 are encapsulated within a sulfated polysaccharide whose external part (i.e., the soluble fraction) dissolves into the medium. It is thought that the main function of the polysaccharide is to protect the algal cells from the extreme environmental conditions, such as drought and high light, prevailing in their native sea-sand habitat. In this study, we evaluated the antioxidant properties of the water-soluble polysaccharide of Porphyridium sp. by determining the ability of a polysaccharide solution to inhibit: (1) autooxidation of linoleic acid, as determined by the standard thiobarbituric acid (TBA) and ferrous oxidation (FOX) assays; and (2) oxidative damage to 3T3 cells as determined by the dichlorofluorescein (DCFH) assay. In all three assays, the polysaccharide inhibited oxidative damage in a dose-dependent manner. Antioxidant activity was also exhibited by fractions of the polysaccharide obtained by sonication followed by separation on a reverse-phase HPLC with a C8 semi-preparative column. It is suggested that the antioxidant activity of the sulfated polysaccharide protects the alga against reactive oxygen species produced under high solar irradiation, possibly by scavenging the free radicals produced in the cell under stress conditions and transporting them from the cell to the medium. 相似文献
16.
Mohsen Mohamed Selim Asker Youssri Mohamed Ahmed Mohamed Fawzy Ramadan 《Carbohydrate polymers》2009,77(3):563-567
The Gram-positive bacterial strain isolated from soil was identified as the non-pathogenic Microbacterium terregens. The exopolysaccharide (CPS) produced from M. terregens was obtained by isopropanol precipitation (13.72 g L?1 growth medium), The resulted exopolysaccharide was purified by chromatography on DEAE-cellulose and Sephacryl S-200 columns, when two polysaccharide fractions termed CPSI and CPSII were obtained. Structure features of CPSI and CPSII were investigated by a combination of chemical and chromatographic analyses, such as acid hydrolysis, methylation analysis, periodate oxidation–Smith degradation, HPLC, GC–MS, and IR. The results indicated that CPSI and CPSII were composed of glucose: mannose in a ratio of 2.7:1 and 3.2:1 with molecular weights 80 and 150 kDa, respectively. It has a backbone of (1 → 4)-linked β-glucose residues, which occasionally branches at O-6. The branches were composed of (1 → 4)-linked β-mannose residues. The antioxidant activity of the CPS, CPSI and CPSII was evaluated in-vitro by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay (RSA). CPSI fraction showed the highest antioxidant activity among the three fractions, with an IC50 value of 230 μg mL?1. The effect of molecular weight of the polysaccharide on the improvement of the antioxidant potential seems to be significant. 相似文献
17.
Shoudong Guo Wenjun Mao Yuanli Li Qianqun Gu Yanli Chen Chunqi Zhao Na Li Chunyan Wang Tiantian Guo Xue Liu 《Process Biochemistry》2013,48(3):539-544
A water-soluble extracellular polysaccharide, designated Os2-1, was isolated from the fermented liquid of the fungus Oidiodendron truncatum GW using ethanol precipitation, anion-exchange and gel-permeation chromatography. Os2-1 was mainly composed of glucose with minor amounts of glucosamine, and its average molecular weight was about 9.6 kDa. On the basis of chemical and spectroscopic analyses, including one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR) spectroscopy, the backbone of Os2-1 was characterized to mainly consist of (1→6)-linked α-d-glucopyranose residues with minor amounts of (1→2)-linked α-d-glucopyranose residues. The antioxidant activity of Os2-1 was evaluated with the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radicals and inhibition effect of lipid peroxidation in vitro, and the results indicated that Os2-1 had good antioxidant activity, especially scavenging ability on DPPH and superoxide radicals. The investigation demonstrated that Os2-1was an extracellular polysaccharide differing from previously described extracellular polysaccharides, and could be a potential antioxidant. 相似文献
18.
Faizan Naeem Razali Amirah Ismail Nurhayati Zainal Abidin Adawiyah Suriza Shuib 《PloS one》2014,9(10)
The polysaccharide fraction from Solanum nigrum Linne has been shown to have antitumor activity by enhancing the CD4+/CD8+ ratio of the T-lymphocyte subpopulation. In this study, we analyzed a polysaccharide extract of S. nigrum to determine its modulating effects on RAW 264.7 murine macrophage cells since macrophages play a key role in inducing both innate and adaptive immune responses. Crude polysaccharide was extracted from the stem of S. nigrum and subjected to ion-exchange chromatography to partially purify the extract. Five polysaccharide fractions were then subjected to a cytotoxicity assay and a nitric oxide production assay. To further analyze the ability of the fractionated polysaccharide extract to activate macrophages, the phagocytosis activity and cytokine production were also measured. The polysaccharide fractions were not cytotoxic, but all of the fractions induced nitric oxide in RAW 264.7 cells. Of the five fractions tested, SN-ppF3 was the least toxic and also induced the greatest amount of nitric oxide, which was comparable to the inducible nitric oxide synthase expression detected in the cell lysate. This fraction also significantly induced phagocytosis activity and stimulated the production of tumor necrosis factor-α and interleukin-6. Our study showed that fraction SN-ppF3 could classically activate macrophages. Macrophage induction may be the manner in which polysaccharides from S. nigrum are able to prevent tumor growth. 相似文献
19.
Zhang L Hu TJ Liu HL Shuai XH 《International journal of biological macromolecules》2011,49(4):607-615
The aim of this study was to investigate the inhibitory effect of Sargassum polysaccharide on oxidative stress induced by infectious bursa disease virus (IBDV) in chicken bursal lymphocytes. The levels of IL-1β, IL-8, IL-10, TNF-α, MCP-1, reduced glutathione and reactive oxygen species in chicken bursal lymphocytes treated with IBDV or both IBDV and Sargassum polysaccharide were measured, and the activities of superoxide dimutase and glutathione peroxidase were evaluated. Our results showed that oxidative stress appeared when chicken bursal lymphocytes were incubated with IBDV for 8 h at 100 TCID50. Sargassum polysaccharide inhibited oxidative stress by increasing the amount of reduced glutathione, promoting the activities of superoxide dimutase and glutathione peroxidase and reducing the level of reactive oxygen species. The polysaccharide also raised IL-1β, IL-8, IL-10 and TNF-α levels in cells infected with IBDV. These findings suggest that Sargassum polysaccharide acts against infection by elevating antioxidant capacity and cytokine levels in chicken bursal lymphocytes. 相似文献
20.
Juçara palm tree is considered an important species in the Atlantic forest ecosystem and the exploitation of its fruits is a main sustainable strategy adopted to its maintenance. Among several microorganisms in the juçara fruits, were found lactic acid bacteria (LAB) highly capable of synthesizing exopolysaccharides (EPS). Thus, the EPS synthesized by Leuconostoc pseudomesenteroides JF17 was characterized according to its chemical structure, physicochemical properties and antioxidant activity. HPLC analysis showed that the EPS consisted of a homopolysaccharide composed of glucose units, FT-IR spectroscopy revealed a dextran, confirmed by 1H and 13C NMR spectra which showed the composition of the polysaccharide, d-glucose units bound by α-(1→6). The EPS showed high water retention capacity, 507.812 ± 47.471 %, and may be related to the porous structure revealed by SEM images. Thermal analysis indicated that the EPS had excellent thermal stability with degradation temperature of 320.8 °C and melting point at 279.81 °C, with the enthalpy of 106.557 J/g. The EPS also presented sequestration ability on different free radicals tested, indicating high antioxidant activity. The results suggest that L. pseudomesenteroides JF17 might be widely used in the production of dextran which has potential qualities for applications in the food industry. 相似文献