Behavioral differentiation of underyearlings of the Black Sea salmon <Emphasis Type="Italic">Salmo trutta labrax</Emphasis>: Rheoreaction in the year preceding smoltification

Hatchery rearing underyearlings of the Black Sea salmon Salmo trutta labrax at the age of five-six months (nine-ten months before the beginning of smoltification) divide into two spatial groups—bottom group and pelagic group. This is caused by lack of territory (bottom area): the minority of fish are bottom-dwelling—such fish are territorial, they extrude the majority of individuals to the water column. It is determined experimentally that the bottom-dwelling fish belonging to the bottom group, in comparison with the fish of pelagic group, are characterized by higher critical flow velocities and lower locomotor activity in the rheogradient. Part of individuals with the static type of rheoreaction among them is higher. The fish from the pelagic group do not have such resource as individual territory. This fact modifies the probability of manifestation of resident and migrant behavior after starvation (during ten days). The individuals from the pelagic group demonstrate the behavior characteristic of future migrants (downstream movement) to a higher degree. The individuals from the bottom group, in comparison with pelagic fish, demonstrate, to a great degree, behavior typical for future residents (upstream movement).     

Parameters of energy metabolism in juveniles of Atlantic salmon <Emphasis Type="Italic">Salmo salar</Emphasis> living in the mainstream and in the tributary of the Varzuga River (the Kola Peninsula)

In juveniles of Atlantic salmon Salmo salar of two age groups (0+ and 1+) living in the mainstream and in the tributary of the subarctic Varzuga River, the activity of some enzymes was determined (cytochrome c oxidase, malate dehydrogenase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase, and 1-glycerophosphate dehydrogenase), reflecting the intensity of the direction of principal pathways of carbohydrate metabolism and of synthesis of ATP. The effect of environmental conditions on growth and development of different age groups is different. Underyearlings (0+) living in the tributary are characterized by an advanced locomotor performance and growth rate. They possess a higher level of aerobic and anaerobic energy metabolism and assimilation of carbohydrates for synthesis of structural and stock compounds in comparison with yearlings living in the mainstream. No significant differences are found between two-summer-old fish (1+) from different habitats in parameters of energy metabolism. This demonstrates that the living conditions for them in the tributary are not so favorable as from underyearlings. The food items in the tributary are small and numerous, i.e., are more available for salmon underyearlings. Parrs 1+ feed on large invertebrates and feeding in the mainstream is preferable. The differences in parameters of energy metabolism of juvenile salmon manifesting themselves in the first year of life make the basis for the fact that the subsequent smoltification of parrs and migration for feeding occur at different ages—2+, 3+, or 4+. This contributes to the formation of the complex age structure of the Varzuga stock of salmon.     

On methods of determination of the rheoreaction type in fish

Type of rheoreaction is investigated in some species of salmonids, cyprinids, and percids. Three types of rheoreaction are discerned: positive (the fish prefer to move against the current), static (the fish retain their position in the current), and negative (the fish prefer to move downstream). It is shown that the type of rheoreaction is probabilistic and is determined by the motivational state of fish. Procedures used in investigation of the rheoreaction type are discussed.     

Rheoreaction type of early juveniles of sockeye salmon Oncorhynchus nerka in the Ozernaya River and Lake Kurilskoe

Rheoreaction of early juveniles of sockeye salmon Oncorhynchus nerka is experimentally investigated, including different spatial groups (denatant migrants, contranatant migrants, specimens living in bays and at closed river banks, juveniles from the coastal zone of the lake). Investigation is made in water of the river flowing from the lake and in water of the stream flowing into the lake. It is shown that the rheoreaction (the type of rheoreaction) is one of behavioral mechanisms performing feeding migration of early juveniles of sockeye salmon. Contranatant migrants that reached the feeding lake retain the positive type of rheoreaction, which favors the exit of juveniles from the zone of discharge exclusion and prevents the drift of juveniles to the river at night at poorer conditions for orientation. Juveniles placed into the water of the inflowing stream manifest a sharply negative rheoreaction type contributing to their rapid lakeward migration for feeding. In water of two steams (taking its beginning in the lake and flowing into it) such type of rheoreaction is observed which ensures migration of sockeye juveniles to their principal feeding water body-Lake Kurilskoe.     

Patterns of movements of fish with negative type of rheoreaction

Experiments carried out on underyearlings of four fish species—roach Rutilus rutilus, carp Cyprinus carpio, bream Abramis brama, and perch Perca fluviatilis—at illumination of 200–300 lx showed that the specimens with negative type of rheoreaction move downstream mainly (47–93%) in the active form, with their heads downstream, and rarer (3–37%) in the active-passive form, with their tails downstream. Increase of motivation for downstream movement caused by starvation leads to a higher part of fish with an active form of movement and to greater speed of movement of underyearlings. Active downstream movement is a manifestation of rheoreaction.     

The Interaction between Water Currents and Salmon Swimming Behaviour in Sea Cages

Positioning of sea cages at sites with high water current velocities expose the fish to a largely unknown environmental challenge. In this study we observed the swimming behaviour of Atlantic salmon (Salmo salar L.) at a commercial farm with tidal currents altering between low, moderate and high velocities. At high current velocities the salmon switched from the traditional circular polarized group structure, seen at low and moderate current velocities, to a group structure where all fish kept stations at fixed positions swimming against the current. This type of group behaviour has not been described in sea cages previously. The structural changes could be explained by a preferred swimming speed of salmon spatially restricted in a cage in combination with a behavioural plasticity of the fish.     

Development and application of methods for investigating the ratio of rheoreaction types of fish in a circular tank

Methods for evaluating the motivational component of the fish rheoreaction (ratio of rheoreaction types) are developed and tested in a circular hydrodynamic tank for three fish species (Danio rerio, Poecilia reticulata, and Inpaichthys kerri). Three rheoreaction types of fish were distinguished: a positive type (fish movements against water current), a negative type (fish movements along with water current), and a compensatory type (fish maintaining their position in relation to immobile reference points). The main distinguishing features of these new methods are unrestricted distance for fish movements, continuous monitoring of individual fish movements (video recording), and evaluation of ratio between the types of rheoreaction in terms of duration of demonstration of these types by each. The minimum required observation time and water current velocities are determined. It is shown that these methods can be used for investigating the types of rheoreaction in different fish species. Every single individual repeatedly demonstrated all these three rheoreaction types during the test.     

Determination of body shape variation in Irish hatchery‐reared and wild Atlantic salmon

Discriminant function analysis was used to distinguish morphologically between samples of parr, smolts and adult Atlantic salmon Salmo salar from several hatchery and river systems in Ireland. The effect of habitat shift was investigated in Atlantic salmon parr. Parr grown from the eyed‐egg stage with a non‐sibling group in a hatchery environment, came to resemble the mean body shape of their host hatchery Atlantic salmon stock more closely than that of a full sibling group grown at their natal hatchery. Wild Atlantic salmon smolts differed in shape from hatchery‐reared smolts. This difference was less pronounced, but still statistically significant when wild adults were compared with hatchery‐reared adults captured in the coastal drift‐net fishery after a year spent at sea. Rearing conditions had a significant impact on the production and growth of fish body shape. This in turn may have affected adaptability and survivorship of ranched Atlantic salmon in the marine environment.     

Factors affecting river entry of adult Atlantic salmon in a small river

In this study, effects of stock origin, fish size, water flow and temperature on time of river ascent of adult Atlantic salmon Salmo salar were tested. Brood stocks were collected in eight Norwegian rivers situated between 59 and 69° N. The fish were reared to smolts, individually tagged and released in the River Imsa, south-west Norway (59° N). Adults from all stocks approached the Norwegian coast concurrently, but Atlantic salmon ≥70 cm in natural tip length entered coastal water slightly earlier during summer than smaller fish. Atlantic salmon <70 cm, however, ascended the river significantly earlier and at lower water flow and higher water temperature than larger fish. Although largest in size, the fish from the northern populations (62–69° N) ascended the River Imsa almost 1 month earlier than those from the south (59–60° N). They seemed less restricted by the environmental factors than the fish originating from the more southern rivers. There was no apparent trend among years in time of river ascent. Maximum ascent per day occurred at water discharges between 12·5 and 15 m³ s⁻¹ and at water temperatures between 10 and 12·5° C. There was a significant positive correlation between water flow and river ascent during the first part of the upstream run from July to September with best correlation for September, when multiple regression analysis indicated that water temperature had an additional positive effect. Stock origin, fish size and water discharge were important variables influencing the upstream migration of Atlantic salmon in small rivers.     

Thyroid hormone levels in Atlantic salmon (Salmo salar) during the return migration from the ocean to spawn

Serum thyroid hormone levels were determined in adult Atlantic salmon ( Salmo salar ) of both sexes caught in the ocean and at a sequence of locations on their return migration to spawn. Tri-iodothyronine (T₃) levels were greatest in fish caught in coastal or estuarine waters or in a river near head-of-tide. T₃ levels were lower in fish caught in rivers throughout the angling season and lowest in those captured entering a tributary near spawning. Thyroxine (T₄) levels were lowest in immature fish captured in the ocean in winter but raised in fish captured in spring; many of the latter group showed endocrine evidence of their becoming sexually mature. T₄ levels were greatest in fish captured in coastal waters and progressively lower in fish captured in an estuary, near head-of-tide and in rivers. T₄ levels in fish captured at tributary entry near spawning exceeded those in fish caught in rivers earlier in the year. In general, these data support the hypothesis that motor activity level in migrant fish is a determinant of thyroid status.     

The feeding behaviour of juvenile Atlantic salmon in relation to turbulent flow

The feeding behaviour of juvenile Atlantic salmon Salmo salar in the Sainte‐Marguerite River, Quebec, Canada, varied with the characteristics of turbulent flow. Simulations indicated that juveniles would decrease their swimming costs during attacks by 19·8% in low and by 31·1% in high turbulent conditions by initiating movements in low‐speed flow events. The real swimming costs did not differ from the swimming costs estimated for a situation where fish initiate their movements at randomly selected flow velocities. The juvenile Atlantic salmon did not seem to prefer low‐speed flow events when initiating their movements. The proportion of time used for movements by fish decreased with an increase in the mean and the s . d . of the flow velocity.     

Type of rheoreaction in the early ontogenesis of roach <Emphasis Type="Italic">Rutilus rutilus</Emphasis> (L.) as a factor determining its biotopic distribution

The specific features of rheoreaction in roach Rutilus rutilus (L.) larvae from open and protected shallows of the Rybinsk Reservoir have been studied. Data on length and weight of roach sampled at various habitats at B–E developmental stages are presented. It has been revealed that the type of roach larvae rheoreaction is a behavioral mechanism of their distribution over different types of habitats, not only in rivers but also in the waterbodies with slow water turnover rates—lakes and reservoirs. By the time of developmental stage E, this mechanism provides the sustainable spatial separation of juvenile fish, which in the future may lead to intrapopulation differentiation in roach.     

Differential expression of Atlantic salmon thyrotropin β subunit mRNA and its cDNA sequence

To study the regulation of the thyroid system, an Atlantic salmon Salmo salar cDNA clone was isolated for thyroid stimulating hormone (TSH) β subunit gene. A cDNA (866 bp) was isolated from an adult Atlantic salmon pituitary cDNA library, this clone was sequenced and shown to be highly conserved when compared to other teleost β TSH subunit sequences. The cDNA was used as a probe for Northern blot analysis of total pituitary RNA from the different life cycle stages of Atlantic salmon. Northern blot analysis demonstrated that β TSH mRNA is expressed at all life cycle stages studied, including parr, smolt, immature fish at sea and sexually mature male fish. Densitometry of Northern blots showed that sexually mature male salmon had low levels of salmon β TSH mRNA compared to non-mature fish. Stunts, fish performing poorly in salt water, were shown to have elevated levels of β TSH mRNA when compared to healthy fish.     

Migration of farmed adult Atlantic salmon with and without olfactory sense, released on the Norwegian coast

The dispersal and migration of farmed Atlantic salmon, Salmo salar , allowed to escape during the summer was studied. Three groups of 4–year–old fish of the River Imsa stock were released in coastal waters off south-western Norway: one group, with functional olfactory organs, was released at a fish farm 4 km away from the R. Imsa; two other groups, one with transected olfactory nerves and the other with functional olfactory organs, were released in the sea 90 km from the R. Imsa. To compare them with the migration pattern of reared, large smolts of the Imsa stock, a group of 3 + smolts was released in the R. Imsa.
Adults of salmon released as 3–year–old smolts homed with high precision to the R. Imsa. Four– year–olds released in the sea were recaptured in the fjord and in the coastal current, the majority north of the places of release. Immatures migrated to feeding areas in the North Atlantic. Matures seemed to enter rivers at random when ready to spawn. There was no difference in migration pattern between anosmics and controls. The olfactory sense was not mandatory for entering fresh water. The results indicate that the homing behaviour of Atlantic salmon is not a direct consequence of a single imprinting of the smolts, and that there is not a direct genetic link for return to a particular river. The present results support the sequential imprinting hypothesis proposed by Harden Jones (1968).     

Natural Colonization and Establishment of a Chinook Salmon, <Emphasis Type="Italic">Oncorhynchus tshawytscha</Emphasis>, Population in the Santa Cruz River,an Atlantic Basin of Patagonia

Synopsis We report the finding of an established population of exotic Chinook salmon spawning in headwaters of the Santa Cruz River system (Argentina), the first for this species in an Atlantic basin of South America. Spawning takes place in the Caterina River, a small tributary of Lake Argentino, located 488.5 km from the ocean. Anadromy was verified by correspondence of N and C stable isotope ratios with those of fish captured by bottom-trawlers in the ocean and those of anadromous rainbow trout from the same river basin. The scale patterns of most fish examined were consistent with a stream-type life cycle (i.e., seaward migration by juveniles after a full year in fresh water). Two potential origins were identified for this population: in situ introductions of fish imported directly from the USA in the early 20th century or fish from two ranching experiments conducted in southern Chile during the 1980s. In the latter case, colonization would have proceeded through the Strait of Magellan, helped by prevailing eastward currents.     

Migration speeds and orientation of Atlantic salmon and sea trout post-smolts in a Norwegian fjord system

We recorded the observed and actual swimming speeds of Atlantic salmon and sea trout post-smolts in a Norwegian fjord system, and initiated studies on the orientation mechanisms of the post-smolts. We tracked Atlantic salmon and sea trout with acoustic transmitters for up to 14 h after release. The actual swimming speed and direction of a fish relative to the ground is the vector sum of the observed movements of the fish and the movements of the water. We determined actual swimming speeds and directions of the post-smolts, which reflect their real swimming capacities and orientation, by corrections for the speed and direction of the water current. The post-smolts were actively swimming. The observed direction of movement was dependent on the actual movement of the fish and not the water current. Water currents were not systematically used as an orientation cue either in Atlantic salmon or sea trout, as the actual movements were random compared to the direction of the water current. The actual movement of sea trout were in all compass directions, with no systematic pattern. The Atlantic salmon also moved in all compass directions, but with the lowest frequency of actual movement towards the fjord.     

Development of spinal deformities in Atlantic salmon and Arctic charr fed diets supplemented with oxytetracycline

Some individuals within populations of Atlantic salmon Salmo salar and Arctic charr Salvelinus alpinus fed diets supplemented with oxytetracycline (OTC) developed spinal deformations. Possible differences in feed intake and growth of spinally deformed fish relative to fish without any deformities were investigated. Amongst Atlantic salmon, 17% of the fish fed OTC-supplemented feed developed spinal fractures, whereas none of the fish receiving the basic feed did so. Despite deformation of the spinal column, the injured fish continued to feed and grow, but at lower rates than unaffected individuals. In contrast to Atlantic salmon, Arctic charr showed no signs of spinal fractures at any time during the 65-day experiment.     

Migration patterns and navigation cues of Atlantic salmon post-smolts migrating from 12 rivers through the coastal zones around the Irish Sea

The freshwater phase of the first seaward migration of juvenile Atlantic salmon (Salmo salar) is relatively well understood when compared with our understanding of the marine phase of their migration. In 2021, 1008 wild and 60 ranched Atlantic salmon smolts were tagged with acoustic transmitters in 12 rivers in England, Scotland, Northern Ireland and Ireland. Large marine receiver arrays were deployed in the Irish Sea at two locations: at the transition of the Irish Sea into the North Atlantic between Ireland and Scotland, and between southern Scotland and Northern Ireland, to examine the early phase of the marine migration of Atlantic salmon smolts. After leaving their natal rivers' post-smolt migration through the Irish Sea was rapid with minimum speeds ranging from 14.03 to 38.56 km.day⁻¹ for Atlantic salmon smolts that entered the Irish Sea directly from their natal river, to 9.69–39.94 km.day⁻¹ for Atlantic salmon smolts that entered the Irish Sea directly from their natal estuary. Population minimum migration success through the study area was strongly correlated with the distance of travel, populations further away from the point of entry to the open North Atlantic exhibited lower migration success. Post-smolts from different populations experienced different water temperatures on entering the North Atlantic. This was largely driven by the timing of their migration and may have significant consequences for feeding and ultimately survivorship. The influence of water currents on post-smolt movement was investigated using data from previously constructed numerical hydrodynamic models. Modeled water current data in the northern Irish Sea showed that post-smolts had a strong preference for migrating when the current direction was at around 283° (west-north-west) but did not migrate when exposed to strong currents in other directions. This is the most favorable direction for onward passage from the Irish Sea to the continental shelf edge current, a known accumulation point for migrating post-smolts. These results strongly indicate that post-smolts migrating through the coastal marine environment are: (1) not simply migrating by current following (2) engage in active directional swimming (3) have an intrinsic sense of their migration direction and (4) can use cues other than water current direction to orientate during this part of their migration.     

Upstream passage problems for wild Atlantic salmon (<Emphasis Type="Italic">Salmo salar</Emphasis> L.) in a regulated river and its effect on the population

Due to hydropower development, the upstream migration of wild anadromous salmon and brown trout is impaired in many European rivers, causing negative effects on the long-term survival of natural salmonid populations. This study identified problems for Atlantic salmon during upstream migration in a regulated river in northern Sweden, Umeälven (mean flow: 430 m³ s^?1). Tagging from 1995 to 2005 involved radio tags (n = 503), PIT tags (n = 1574) and Carlin tags (n = 573) to study the spawning migration of salmon from the coast past the regulated section of the river to a fish ladder at the dam/spillway 32 km upriver. The results demonstrate that migration success from the coast to the fish ladder varied between 0% and 47% among years, indicating an average loss of 70% of potential spawners. Discharge from the turbines attracted the salmon away from the bypass route. Echo-sounding in the turbine outlet showed that salmon were normally found at 1–4 m depths. They responded with upstream and/or downstream movements depending on flow changes; increased spill in the bypass channel attracted salmon to the bypass. Once in the bypass channel, salmon could be delayed and had difficulties passing the first rapid at high spills. Additional hindrances to upstream migration were found at rapids and the area of the fish ladder, located further upstream in the regulated river section. The average migration duration was 44 days from the estuary to the top of the fish ladder, with large variation among individuals within years. Modelling the salmon population dynamics showed a potential population increase of 500% in 10 years if the overall migration success could be improved from the current 30% to levels near 75%. Consequently improved migration facilities at the regulated river section should be implemented to achieve a long-term sustainability of these threatened anadromous salmonids.     

Analysis of correlation between body length and genetic polymorphism for loci ESTD-1 and IDHP-3 in the Atlantic salmon Salmo salar

Correlation between body length and characteristics of genetic polymorphism for loci Me-2, IDHP-3, ESTD-1, and AAT-2 were studied in the Atlantic salmon Salmo salar. The study was based on data on genetic variation in more than 4000 fish grown at the Kandalaksha Hatchery. Of four loci studied, loci ESTD-1 and IDHP-3 were distinguished by the fact that they can be confidently considered body-length markers in the Atlantic salmon. Analysis of mean locus heterozygosity indicates a disturbance of the mechanism of negative heterosis that is characteristic of natural populations. This presumably reflects a change in the genetic diversity of the Atlantic salmon under the influence of breeding.