共查询到20条相似文献,搜索用时 15 毫秒
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Smith-Espinoza CJ Phillips JR Salamini F Bartels D 《Molecular genetics and genomics : MGG》2005,274(4):364-372
The resurrection plant (Craterostigma plantagineum) is desiccation tolerant. However, callus derived from this plant, when propagated in vitro, requires exogenously applied
abscisic acid (ABA) in order to survive desiccation. Treatment of callus tissue with ABA induces most of the genes that are
induced by dehydration in the whole plant. This property has been exploited for the isolation of mutants that show dominant
phenotypes resulting from the ectopic expression of endogenous genes induced by the insertion of a foreign promoter. Here
we describe new T-DNA tagged Craterostigma desiccation-tolerant (cdt) mutants with different molecular and physiological characteristics, suggesting that different pathways of desiccation tolerance
are affected. One of the mutants, cdt-2, constitutively expresses known osmoprotective Lea genes in callus and leaf tissue. Further analysis of this mutant revealed that the tagged locus is similar to a previously
characterised gene, CDT-1, which codes for a signalling molecule that confers desiccation tolerance. The nature of the T-DNA insertion provides insight
into the mechanism by which the CDT-1/2 gene family functions in ABA signal transduction. 相似文献
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Andrea Ditzer Hans-Hubert Kirch Ambili Nair Dorothea Bartels 《Journal of plant physiology》2001,158(5):623
Expression of many genes is induced during dehydration in vegetative tissues of the desiccation tolerant resurrection plantCraterostigma plantagineum. The most abundant group of desiccation-related gene products belong to the LEA (= Late Embryogenesis Abundant) proteins. Here we describe structures and expression patterns of members of group 3 and group 4Lea genes fromC. plantagineum. The most intriguing observation is the strong conservation of repeat motifs inLea genes found across divers plant species includingC. plantagineum and non-desiccation tolerant plants. This conservation of structural elements leads to speculations about evolution of desiccation tolerance in the resurrection plant. 相似文献
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Bartels D 《Integrative and comparative biology》2005,45(5):696-701
This review will focus on the acquisition of desiccation tolerancein the resurrection plant Craterostigma plantagineum. Molecularaspects of desiccation tolerance in this plant will be comparedwith the response of non-tolerant plants to dehydration. Uniquefeatures of C. plantagineum are described like the CDT-1 (Craterostigmadesiccation tolerance gene-1) gene and the carbohydrate metabolism.Abundant proteins which are associated with the desiccationtolerance phenomenon are the late embryogenesis abundant (=LEA)proteins. These proteins are very hydrophilic and occur in severalother species which have acquired desiccation tolerance. 相似文献
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Röhrig H Schmidt J Colby T Bräutigam A Hufnagel P Bartels D 《Plant, cell & environment》2006,29(8):1606-1617
Reversible phosphorylation of proteins is an important mechanism by which organisms regulate their reactions to external stimuli. To investigate the involvement of phosphorylation during acquisition of desiccation tolerance, we have analysed dehydration-induced protein phosphorylation in the desiccation tolerant resurrection plant Craterostigma plantagineum. Several dehydration-induced proteins were shown to be transiently phosphorylated during a dehydration and rehydration (RH) cycle. Two abundantly expressed phosphoproteins are the dehydration- and abscisic acid (ABA)-responsive protein CDeT11-24 and the group 2 late embryogenesis abundant (LEA) protein CDeT6-19. Although both proteins accumulate in leaves and roots with similar kinetics in response to dehydration, their phosphorylation patterns differ. Several phosphorylation sites were identified on the CDeT11-24 protein using liquid chromatography-tandem mass spectrometry (LCMS/MS). The coincidence of phosphorylation sites with predicted coiled-coil regions leads to the hypothesis that CDeT11-24 phosphorylations influence the stability of coiled-coil interactions with itself and possibly other proteins. 相似文献
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It was recently discovered that plants respond to environmental stress not only with a specific gene expression programme at the mRNA and protein level but also small RNAs as response modulators play an important role. The small RNAs lead to cleavage or translational inhibition of mRNAs via complementary target sites. Different examples are described where small RNAs have been shown to be involved in stress responses. A link between hormonal action and small RNA activities has frequently been observed thus coupling exogenous factors with endogenous transmitters. Using the CDT-1 gene from the desiccation tolerant plant Craterostigma plantagineum as an example, it is discussed that generation of novel small RNAs could be an evolutionary pathway in plants to adapt to extreme environments. 相似文献
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Fisher KM 《American journal of botany》2008,95(4):506-515
Desiccation tolerance is a complex trait that is broadly but infrequently present throughout the evolutionary tree of life. Desiccation tolerance has played a significant role in land plant evolution, in both the vegetative and reproductive life history stages. In the land plants, the late embryogenesis abundant (LEA) gene families are involved in both abiotic stress tolerance and the development of reproductive propagules. They are also a major component of vegetative desiccation tolerance. Phylogenies were estimated for four families of LEA genes from Arabidopsis, Physcomitrella, and the desiccation tolerant plants Tortula ruralis, Craterostigma plantagineum, and Xerophyta humilis. Microarray expression data from Arabidopsis and a subset of the Physcomitrella LEAs were used to estimate ancestral expression patterns in the LEA families and to evaluate alternative hypotheses for the origins of vegetative desiccation tolerance in the flowering plants. The results contradict the idea that vegetative desiccation tolerance in the resurrection angiosperms Craterostigma and Xerophyta arose through the co-option of genes exclusively related to stress tolerance, and support the propagule-derived origin of vegetative desiccation tolerance in the resurrection plants. 相似文献
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Hilbricht T Salamini F Bartels D 《The Plant journal : for cell and molecular biology》2002,31(3):293-303
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Reversible protein phosphorylation/dephosphorylation is crucial for regulation of many cellular events, and increasing evidence indicates that this post-translational modification is also involved in the complex process of acquisition of desiccation tolerance. To analyze the phosphoproteome of the desiccation tolerant resurrection plant Craterostigma plantagineum, MOAC-enriched proteins from leaves at different stages of a de-/rehydration cycle were separated by 2-D PAGE and detected by phosphoprotein-specific staining. Using this strategy 20 putative phosphoproteins were identified by MALDI-TOF MS and MS/MS, which were not detected when total proteins were analyzed. The characterized desiccation-related phosphoproteins CDeT11-24 and CDeT6-19 were used as internal markers to validate the specificity of the analyses. For 16 of the identified proteins published evidence suggests that they are phosphoproteins. Comparative analysis of the 2-D gels showed that spot intensities of most identified putative phosphoproteins change during the de-/rehydration cycle. This suggests an involvement of these proteins in desiccation tolerance. Nearly all changes in the phosphoproteome of C. plantagineum, which are triggered by dehydration, are reversed within 4 days of rehydration, which is in agreement with physiological observations. Possible functions of selected proteins are discussed in the context of the de-/rehydration cycle. 相似文献
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Dan Pelah Wangxia Wang Arie Altman Oded Shoseyov Dorothea Bartels 《Physiologia plantarum》1997,99(1):153-159
Proteins inducible by dehydration and abscisic acid (ABA), have been identified in a number of species and have been suggested to play a role in desiccation tolerance. Recently, we identified a novel boiling-stable protein (BspA) which accumulated in shoots of aspen ( Populus tremula L.) cultured in vitro, in response to gradual water stress and ABA application (Pelah et al. 1995. Tree Physiol. 15: 673–678.). Accumulation of BspA, and of the water stress-related protein dehydrin dsp- 16 and sucrose synthase from the resurrection plant. Craterostigma plantagineum , was examined in two greenhouse-grown Populus species to investigate the relationship between the presence of the proteins and water stress tolerance. Detached leaves of Populus tomentosa lost more water than Populus popularis , resulting in a significant decrease in leaf water potential. Using electrolyte leakage analysis, it was found that detached leaves of Populus popularis are more tolerant to water stress than those of Populus tomentosa . Using western blots with the corresponding antibodies, we have found in Populus popularis accumulation of BspA and sucrose synthase due to water stress, and the constitutive presence of a dehydrin-like protein. In contrast, a low expression of BspA was found in Populus tomentosa , but not of sucrose synthase and dehydrin-like proteins. Desiccation tolerance in many tissues can be partly attributed to soluble sugars. Analysis of the amount of soluble sugars did not reveal clear-cut differences between the two species, except for significant sucrose accumulation and glucose reduction in water-stressed Populus tomentosa and increase in glucose in water-stressed Populus popularis . The data obtained points to a positive correlation between increased water stress tolerance of one poplar species as compared with another and accumulation of water stress-related proteins and sucrose synthase. 相似文献