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C P Verrijzer J A van Oosterhout W W van Weperen P C van der Vliet 《The EMBO journal》1991,10(10):3007-3014
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The ubiquitously expressed mammalian POU-domain protein Oct-1 specifically recognizes two classes of cis-acting regulatory elements that bear little sequence similarity, the octamer motif ATGCAAAT and the TAATGARAT motif. The related pituitary-specific POU protein Pit-1 also recognizes these two motifs but, unlike Oct-1, binds preferentially to the TAATGARAT motif. Yet in our assay, Pit-1 still binds octamer elements better than does the octamer motif-binding protein Oct-3. The POU domain is responsible for recognizing these diverse regulatory sequences through multiple DNA contacts that include the two POU subdomains, the POU-specific region, and the POU homeodomain. The DNA-binding properties of 10 chimeric POU domains, in which different POU-domain segments are derived from either Oct-1 or Pit-1, reveal a high degree of structural plasticity; these hybrid proteins all bind DNA well and frequently bind particular sites better than does either of the parental POU domains. In these chimeric POU domains, the POU-specific A and B boxes and the hypervariable POU linker can influence DNA-binding specificity. The surprising result is that the influence a particular segment has on DNA-binding specificity can be greatly affected by the origin of other segments of the POU domain and the sequence of the binding site. Thus, the broad but selective DNA-binding specificity of Oct-1 is conferred both by multiple DNA contacts and by dynamic interactions within the DNA-bound POU domain. 相似文献
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The DNA binding specificity of the bipartite POU domain and its subdomains. 总被引:15,自引:0,他引:15
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C P Verrijzer M J Alkema W W van Weperen H C Van Leeuwen M J Strating P C van der Vliet 《The EMBO journal》1992,11(13):4993-5003
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Developmental Effects of Ectopic Expression of the Glucocorticoid Receptor DNA Binding Domain Are Alleviated by an Amino Acid Substitution That Interferes with Homeodomain Binding
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Jun Ming Wang Gratien G. Prfontaine Madeleine E. Lemieux Louise Pope Marie-Andre Akimenko Robert J. G. Hach 《Molecular and cellular biology》1999,19(10):7106-7122
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