Modulation of leaf elongation, tiller appearance and tiller senescence in spring barley by far-red light

Supplemental far-red (FR) illumination of light-grown grass seedlings inhibits tiller production while enhancing leaf elongation. Although much is known about FR enhancement of internode elongation in dicots, relatively little research has been conducted to determine the effects of FR on monocot development. In growth chamber experiments, fibre optics were used to direct supplemental FR to elongating leaf blades, main stem bases and mature leaf blades of light-grown barley (Hordeum vulgare L.) seedlings. Our objective was to identify specific sites of perception for FR enhancement of leaf elongation and inhibition of tiller production, and to assess potential FR effects on tiller senescence. Far-red illumination of elongating leaves or of the main stem base reduced the total number of tillers per plant, primarily by reducing secondary and tertiary tiller production, and enhanced leaf elongation. However, leaf elongation was less sensitive to stem base treatments than to illumination of the elongating blade. Increased leaf length resulted from increased leaf elongation rate, while the duration of leaf elongation was unaffected. Exposure of mature leaf blades to FR had no effect on tillering or leaf elongation. None of the FR treatments led to tiller senescence. Localization of FR perception in vertically oriented tissues such as elongating blades and stem bases permits early detection of reflected light from neighbouring plants, allowing rapid response to impending competition.     

The relationship between leaf canopy development and yield of barley

A study was carried out into the relationship between sowing date, canopy development and yield of four barley genotypes sown in autumn and spring. Yield was considered as the product of PAR absorbed by the crop canopy up till anthesis, the dry matter: radiation quotient, the ratio of grains m²at maturity to the weight of the crop at anthesis and the mean grain weight. Equations were derived to predict the time course of the proportion of PAR absorbed in relation to date of sowing using thermal time as predictor. In crops that were not short of water and were fully vernalised, genotypic differences were detected in plant population density, date of anthesis, and the four yield components identified above. All attributes except plant population density were affected by shortage of water but genotypes differed in their response.     

The effect of tiller removal and tiller defoliation on competition between the main shoot and tillers of spring barley

In glasshouse and field experiments the source-sink relations of the main shoot of plants of spring barley were modified by tiller removal and tiller defoliation. Decreasing competition by tiller removal promoted the growth of the residual main shoot and its component parts, and the earlier tillers were removed the greater was the effect. Stem dry weight was increased four-fold in the glasshouse by early tiller removal and was doubled in the field experiment. The grain yield of the main shoot ear was increased by 26 – 30% by tiller removal compared with tillering control plants and this was due to larger grains in all spikelet positions. On the other hand increasing competition by regular tiller defoliation had relatively little effect on the growth and development of the main shoot in the glasshouse study, but in the field the main shoot grain yield was reduced by 10% compared with the control. The main effect of tiller defoliation was on the development of tillers. In the glasshouse tillers survived repeated defoliation, continued to be produced, and the majority produced grain but with fewer and smaller grains per ear than in control plants. Tiller growth was supported by the import of assimilate from the main shoot and this was accompanied by an increase in the photosynthetic rate of the main shoot leaves. In the field all defoliated tillers died within 4 wk. These responses are discussed in terms of the physiological interrelations between the main shoot and tillers.     

Genetics of barley hooded suppression

The molecular basis of the barley dominant Hooded (K) mutant is a duplication of 305 bp in intron IV of the homeobox gene Bkn3. A chemical mutagenesis screen was carried out to identify genetical factors that participate in Bkn3 intron-mediated gene regulation. Plants from recurrently mutagenized KK seeds were examined for the suppression of the hooded awn phenotype induced by the K allele and, in total, 41 suK (suppressor of K) recessive mutants were identified. Complementation tests established the existence of five suK loci, and alleles suKB-4, suKC-33, suKD-25, suKE-74, and suKF-76 were studied in detail. All K-suppressed mutants showed a short-awn phenotype. The suK loci have been mapped by bulked segregant analysis nested in a standard mapping procedure based on AFLP markers. K suppressor loci suKB, B, E, and F all map in a short interval of chromosome 7H, while the locus suKD is assigned to chromosome 5H. A complementation test between the four suK mutants mapping on chromosome 7H and the short-awn mutant lks2, located nearby, excluded the allelism between suK loci and lks2. The last experiment made clear that the short-awn phenotype of suK mutants is due to a specific dominant function of the K allele, a function that is independent from the control on hood formation. The suK loci are discussed as candidate participants in the regulation of Bkn3 expression.     

Temporal development of the barley leaf metabolic response to Pi limitation

The response of plants to P_i limitation involves interplay between root uptake of P_i, adjustment of resource allocation to different plant organs and increased metabolic P_i use efficiency. To identify potentially novel, early‐responding, metabolic hallmarks of P_i limitation in crop plants, we studied the metabolic response of barley leaves over the first 7 d of P_i stress, and the relationship of primary metabolites with leaf P_i levels and leaf biomass. The abundance of leaf P_i, Tyr and shikimate were significantly different between low Pi and control plants 1 h after transfer of the plants to low P_i. Combining these data with ¹⁵N metabolic labelling, we show that over the first 48 h of P_i limitation, metabolic flux through the N assimilation and aromatic amino acid pathways is increased. We propose that together with a shift in amino acid metabolism in the chloroplast a transient restoration of the energetic and redox state of the leaf is achieved. Correlation analysis of metabolite abundances revealed a central role for major amino acids in P_i stress, appearing to modulate partitioning of soluble sugars between amino acid and carboxylate synthesis, thereby limiting leaf biomass accumulation when external P_i is low.     

Photosynthetic leaf area modulates tiller bud outgrowth in sorghum

Shoot branches or tillers develop from axillary buds. The dormancy versus outgrowth fates of buds depends on genetic, environmental and hormonal signals. Defoliation inhibits bud outgrowth indicating the role of leaf‐derived metabolic factors such as sucrose in bud outgrowth. In this study, the sensitivity of bud outgrowth to selective defoliation was investigated. At 6 d after planting (6 DAP), the first two leaves of sorghum were fully expanded and the third was partially emerged. Therefore, the leaves were selectively defoliated at 6 DAP and the length of the bud in the first leaf axil was measured at 8 DAP. Bud outgrowth was inhibited by defoliation of only 2 cm from the tip of the second leaf blade. The expression of dormancy and sucrose‐starvation marker genes was up‐regulated and cell cycle and sucrose‐inducible genes was down‐regulated during the first 24 h post‐defoliation of the second leaf. At 48 h, the expression of these genes was similar to controls as the defoliated plant recovers. Our results demonstrate that small changes in photosynthetic leaf area affect the propensity of tiller buds for outgrowth. Therefore, variation in leaf area and photosynthetic activity should be included when integrating sucrose into models of shoot branching.     

Resistance QTL confirmed through development of QTL-NILs for barley leaf rust resistance

Near-isogenic lines (NILs) differing with regard to disease QTLs provide valuable material for a more detailed study into the genetic basis of quantitative resistance. Previously obtained information on QTLs that show an effect on leaf rust (Puccinia hordei) in barley was used in a marker-assisted backcross programme. The genome origin in backcross plants was controlled through AFLP marker analysis and graphical genotyping. Plants obtained after the third generation of backcrossing sufficiently resembled the recurrent parent. For one QTL, BC₃S₁ plants were evaluated in a disease test and genotyped. NILs containing the desired QTL in homozygous condition in a recipient background were finally obtained. A disease test and verification of the marker genotype confirmed the identity of the NILs. Simultaneous with the backcross programme a simulation study on efficiency of marker-assisted backcrossing was performed.     

Induction of barley leaf urease

Foliar urea application on barley plants increased leaf urease activity for 5 hours with a peak of 20-fold at 2 hours. To discern the mode of urease induction, urea with or without inhibitors and [³⁵S]methionine were incubated with leaf sections for different lengths of time. Urease was extracted, partially purified, electrophoresed, and then quantified by fluorogram. Five urease (U) isozymes were separated by PAGE. U_a and U_b might be polymers or complexes that occurred only at the peak of induced activity. U₁ and U₂ appeared at 0.5 and 0.75 hour, respectively, after urea induction, peaked at 2 hours, and persisted only in treated leaves for several additional hours indicating that they are transient inducible forms. U₃ was the constitutive form present in control and treated leaves. Induction with cordycepin or cycloheximide completely prevented urea stimulated activity and nullified the existence of isozymes U_a, U_b, U₁, and U₂. ³⁵S-U₁, which was labeled in the last hour of induction, appeared on fluorogram 1 hour after induction, peaked at 2 hours, and declined at 3 hours. Results indicated that de novo synthesis of urease is activated by the influx of urea.     

Purification and properties of barley leaf ribonuclease

The principal ribonuclease from young barley plants was purified 29 200-fold by a six-step procedure. The enzyme showed a high specific activity (15 5OO ΔA₂₆₀ units/min/mg protein) and a molecular weight of about 25 000 was indicated by gel filtration and equilibrium sedimentation. Kinetic analysis of the cleavage of dinucleoside monophosphates and of yeast RNA indicated a base preference of Gua > Ade ≥ Ura ? Cyt, and was sensitive to the base located on either side of the phosphodiester bond. The enzyme resembles the Type I class of plant ribonucleases (E.C. 2.7.7.x).     

Genetics and development

A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in genetics and development.     

Genetics and development

A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in genetics and development.     

Genetics and development

A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in genetics and development.     

Genetics and development

A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in genetics and development.     

Genetics and development

A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in genetics and development.     

Genetics and development

A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in genetics and development.     

Phyllochron and winter barley leaf growth rate

Growth rate of leaves of winter barley (Hordeum vulgare L. cv. Efra) plant in thermal time correlated with the final length of the corresponding leaf (r = 0.66). The sum of effective temperatures corresponding to the time of growth of a particular leaf linearly increased with leaf insertion (r = 0.82). Phyllochron value in plants grown in the field with either natural or controlled photoperiod was dependent on phyllochron order, especially up to the double ridge stage (r = 0.89 and r = 0.74, respectively). The mean value of phyllochrons in the field variants showed a very good agreement with the value calculated on the basis of the change in day length at the time of seedling emergence.