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1.
The effects (and interaction) of two solution concentrations of Mg (50, 500, μM) and two of K (250, 4250 μM) on the growth of micropropagated plants of “F. 12/1” and “Colt” were investigated using a flowing solution culture system.
Magnesium inflow and growth of “Colt” and “F. 12/1” were inhibited to a similar extent by an increased concentration of K
in the nutrient solution. However, the consequences of this inhibition were different. Reduced inflow of Mg in “F. 12/1” caused
Mg deficiency symptoms at high and low concentrations of K, whereas this only occurred with a combination of high K concentration
and low Mg concentration in “Colt”. The distribution of dry matter within the plant was significant in determining susceptibility
to Mg deficiency. Since “F. 12/1” has a smaller root:shoot ratio than Colt it is unable to sustain the same concentration
of Mg in leaves as “Colt” irrespective of external K concentration. The molar ratio of K:Mg in soil solutions should remain
<8.5:1 in order to ensure maximum growth of “F. 12/1” and “Colt”.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
2.
Alison Woodcock 《Primates; journal of primatology》1983,24(4):501-514
In a series of five experiments, female/female twin and male/male twin common marmosets were observed in each of the following
social situations: as offspring in their respective family groups; housed together as twins; in competition for a mate and
in the heterosexual pairs resulting from this competition. Quantitative records were made of their behaviour. These were then
related to winning or losing the competition for a mate. Individuals who demonstrated more “assertive” behaviour and won the
competition (winners) were not in every experiment faster to produce and rear offspring than were the less “assertive” animals
(losers). Categories of behaviour described as “assertive” predicted the outcome of the competition in most cases, but were
not good indicators of future breeding potential. 相似文献
3.
Citrus is an important fruit crop as regards accumulation of carotenoids. In plant carotenoid biosynthesis, phytoene synthase gene
(Psy) plays a key role in catalyzing the head-to-head condensation of geranylgeranyl diphosphate molecules to produce colorless
phytoene. In the present paper, we reported the phytoene contents determination and characterization of Psy during fruit ripening of “Washington” navel orange and its red-fleshed mutant “Cara Cara”. Results showed that phytoene was
exclusively accumulated in peel and pulp of “Cara Cara”. Although phytoene was observed accumulating with fruit ripening of
“Cara Cara”, the contents in pulp were 10 times higher than those in peel. The isolated two Psy cDNAs were both 1520 bp in full length, containing 436 deduced amino acid residues, with a different amino acid at 412th.
Genomic hybridization results showed that one or two copies might be present in “Cara Cara” and “Washington” genomes. During
“Cara Cara” and “Washington” fruit coloration, expression of Psy was observed to be up-regulated, as revealed by tissue specific profiles in the flavedo, albedo, segment membrane and juice
sacs. However, Psy expression in albedo of “Cara Cara” was higher than that in “Washington”, as evidenced by phytoene accumulation in the peel. 相似文献
4.
C. L. Carretero M. Cantos J. L. García A. Troncoso 《In vitro cellular & developmental biology. Plant》2007,43(4):364-369
Three cassava clones (SOM-1, “05”, and “50”) were cultured in vitro on MS medium plus sucrose (30 g L−1) and myo-inositol (100 mg L−1) without plant growth regulators and with additions of 0 (control), 0.5, 1, 1.5, 2, 2.5, and 3 g L−1 NaCl to test their salt tolerance. The same cassava clones were cultivated in greenhouse conditions on a sandy soil substratum
and irrigated with 20% strength Hoagland solution, and additions of 0, 4, and 8 g L−1 of NaCl. Salinity negatively affected the survival, development, leaf water content, and mineral composition (mainly by accumulation
of Cl and Na) of both in vitro and ex vitro plants, but with different intensity in each clone. In both conditions of culture (in vitro and ex vitro) clone SOM-1, from a desert arid saline zone of Somalia, was the most tolerant and clone “05”, from a rainy region of Ivory
Coast, the most sensitive. Clone “50” tolerance to in vitro salt treatments, although lower, was not significantly different from that of SOM-1 but the ex vitro response was similar to “05”. In general, there was a correlation between in vitro and ex vitro behavior of the cassava plant regarding salt tolerance, which would allow the in vitro culture method to be used for selection of salt-tolerant plants of this crop. 相似文献
5.
Chang-Gi Kim Dae In Kim Hyo-Jeong Kim Ji Eun Park Bumkyu Lee Kee Woong Park Soon-Chun Jeong Kyung Hwa Choi Joo Hee An Kang-Hyun Cho Young Soon Kim Hwan Mook Kim 《Journal of Plant Biology》2009,52(3):251-258
We conducted a 2-year field assessment of the gene flow from genetically modified (GM) chili pepper (Capsicum annuum L.), containing the PepEST (pepper esterase) gene, to a non-GM control line “WT512” and two commercial hybrid cultivars, “Manidda” and “Cheongpung Myeongwol
(CM).” After seeds were collected from the pollen-recipient non-GM plants, hybrids between them and the GM peppers were screened
by a hygromycin assay. PCR with the targeting hpt gene was performed to confirm the presence of transgenes in hygromycin-resistant seedlings. Out of 7,071 “WT512” seeds and
6,854 “Manidda” seeds collected in 2006, eight and 12 hybrids, respectively, were detected. In 2007, 33 hybrids from 3,456
“WT512” seeds and 50 hybrids from 3,457 “CM” seeds were found. The highest frequency of gene flow, 6.19%, was observed in
that 2007 trial. These results suggest that a limited isolation distance would be sufficient to prevent gene flow from GM
to conventionally bred chili peppers. 相似文献
6.
Field grown foliage from the resistant soybean [Glycine max (L.) Merrill] breeding line GAT “81–327” and the susceptible cultivar “Ransom” was used to rear unparasitized larvae of the
soybean looper (SBL),Pseudoplusia includens (Walker), and larvae parasitized byCopidosoma truncatellum (Dalman). SBL larvae, whether parasitized or not, consumed more foliage when fed “Ransom”. Unparasitized larvae reared on
“81–327” had longer developmental times and suffered greater mortality than unparasitized larvae reared on “Ransom”. Parasitization
of SBL larvae byC. truncatellum increased total foliage consumption of both soybean lines. Parasitized larvae reared on the resistant “81–327” weighed less
and yielded fewer parasitoid adults.
Résumé Des larves dePseudoplusia includens (Walker) parasitées ou non parCopidosoma truncatellum (Dalman) ont été nourries des feuilles de deux lignées de soja [Glycine max (L.) Merrill] l'une, GAT “81–327” résistante et l'autre, “Ransom” sensible. Les larves deP. includens qu'elles soient parasitées ou non consommaient plus de feuillage lorsqu'elles étaient nourries de la lignée “Ransom”. Les larves non parasitées élevées sur “81–327” avaient un cycle de développement beaucoup plus long et un taux de mortalité beaucoup plus élevé que les larves non parasitées élevées comparativement sur feuilles de “Ransom”. Par contre, les larves parasitées manifestaient une consommation accrue du feuillage des deux lignées de soja. Les larves parasitées élevées sur les feuilles de la variété résistante GAT “81–327” pesaient moins et produisaient moins également de parasites adultes.相似文献
7.
M Mizianty W Bieniek A Czech W Strzaτka M Szklarczyk 《Plant Systematics and Evolution》2005,256(1-4):193-200
The AFLP method was used to study the inter-population variability of eight populations of Elymus caninus (L.) L. as well as three populations of Hordelymus europaeus (L.) Jess. ex Harz. In these studies a clear distinction was found between two phenotypes of E. caninus collected in the same locality. It also appeared that two populations of E. caninus representing the “pauciflorum” morphotype were clustered together, similarly as two populations of E. caninus exhibiting morphotype “caninus”. Additionally, the populations of the “pauciflorum” type were clustered together with all
samples of H. europaeus. Furthermore, the same approach was applied to analyze the intra-population variability of E. caninus. The populations ranged from nearly uniform to as diverse as the samples collected from different localities. In some populations
of this species the presence of off-type plants was revealed. Our data indicate the predominantly self-pollinating character
of E. caninus and the possible genetic relationship between of E. caninus and H. europaeus.
It is the second paper from the series: Biodiversity of wild Triticeae (Poaceae) in Poland, the first is: Mizianty M. 2005.
Variability and structure of natural populations of Elymus caninus (l.) L. based on morphology. Pl. Syst. Evol. 251: 199–216. 相似文献
8.
Chang-Gi Kim Bumkyu Lee Dae In Kim Ji Eun Park Hyo-Jeong Kim Kee Woong Park Hoonbok Yi Soon-Chun Jeong Won Kee Yoon Chee Hark Harn Hwan Mook Kim 《Journal of Plant Biology》2008,51(1):74-77
Gene flow from genetically modified (GM) crops to conventional non-GM crops is a serious concern for protection of conventional
and organic farming. Gene flow from GM watermelon developed for rootstock use, containing cucumber green mottle mosaic virus
(CGMMV)-coat protein (CP) gene, to a non-GM isogenic control variety “Clhalteok” and grafted watermelon “Keumcheon” was investigated
in a small scale field trial as a pilot study. Hybrids between GM and non-GM watermelons were screened from 1304 “Chalteok”
seeds and 856 “Keumcheon” seeds using the duplex PCR method targeting theCGMMV- CP gene as a marker. Hybrids were found in all pollen recipient plots. The gene flow frequencies were greater for “Chaiteok”
than for “KeumcheonD; with 75% outcrossing in the “Chaiteok” plot at the closest distance (0.8 m) to the GM plot. A much larger
scale field trial is necessary to identify the isolation distance between GM and non-GM watermelon, as the behaviors of insect
pollinators needs to be clarified in Korea. 相似文献
9.
Den sharing by conspecific spiny lobsters (aggregation) is modulated by chemical attraction but may confer several, not necessarily
mutually exclusive, antipredator byproduct benefits: a “guide effect”, which only benefits the individual attracted to a sheltered
conspecific; a “dilution effect”, which reduces per-capita risk of predation simply through aggregation; or active “group
defense”. Each potential benefit has a different set of predictors (relationships between aggregation and conspecific or predator
densities), but conflicting results could suggest the simultaneous operation of more than one benefit. These predictions were
tested for coexisting Panulirus guttatus (a reef-obligate) and Panulirus argus (a temporary reef-dweller) using data collected during 11 surveys on fixed sites over a coral reef in Mexico. P. guttatus greatly outnumbered P. argus, but P. argus showed a greater tendency to aggregate. All three benefits of den sharing operated for the more social P. argus, with “group defense” being of the most benefit for larger individuals, and the “guide” and “dilution” effects for smaller
individuals recently immigrating into the reef habitat and sharing dens with larger conspecifics. P. guttatus did not display “group defense” and its aggregations appeared to be modulated by the interplay between attraction and aggressive
behaviors. This species relied more on solitary crypticity, especially at larger sizes, but appeared to benefit from a “guide
effect” at high conspecific densities. In experimental tanks, each species tended to aggregate when tested separately, but
when tested jointly, aggregation among P. guttatus was significantly reduced. The experimental results reflect the differential patterns of aggregation between the fore-reef,
where P. guttatus dominated, and the back-reef, where coexistence of both species was greater. 相似文献
10.
George D. Koufos Louis de Bonis Dimitris S. Kostopoulos Laurent Viriot Theodora D. Vlachou 《Pal?ontologische Zeitschrift》2004,78(1):213-228
Two skulls, two mandibles and some postcranials ofMesopithecus from the locality “Vathylakkos 2” of Axios Valley (Macedonia, Greece) enlarge our knowledge about the genus and its differentiation.
The new material together with an old skull from the same locality is compared with the Pikermi, “Ravin des Zouaves 5” (Axios
Valley), and Maramena (Serres basin) samples. It is concluded that the VathylakkosMesopithecus resemblesM. pentelicus from Pikermi, as wellM. delsoni from “Ravin des Zouaves 5” and it is referred asMesopithecus sp. aff.M. pentelicus. The biochronological age of “Vathylakkos 2” has been considered as MN 12, while recent magnetostratigraphic data indicate
an age of about 7.5 Ma. This age confirms the position of the VathylakkosMesopithecus between Pikermi and “Ravin des Zouaves 5”. Some dental indices compared with those of the RecentCercopithecus suggest that the Vathylakkos sample is monospecific.
相似文献
11.
We observed a case of infant adoption in an unprovisioned group of wild black-fronted titi monkeys (Callicebus nigrifrons). During our long-term field study we noticed that an infant had moved from one of our study groups (“Desbotado”) to another
(“Rio”). Observations of the adoptive group confirm that it was being cared for by the adult male, and initially the group’s
adult female was nursing the infant alongside her biological infant. Interestingly, the native and adoptive groups have frequent
inter-group interactions, but at no point have we observed the native group trying to retrieve its infant. As of April 2007
the infant has been living in its adoptive group for 19 months. These data document the first case of adoption in this genus;
they suggest that infant recognition is poorly developed in this species and that under certain circumstances wild groups
of C.
nigrifrons can successfully rear twins. In our study population reproductive females give birth to one infant every year; the only case
when this has not happened is with the group that adopted the infant, suggesting that adoption may generate a reproductive
cost. 相似文献
12.
The genetic diversity and genetic relatedness of mei (Prunus mume; 2n = 16) were studied using amplified fragment length polymorphism (AFLP) markers. Eight EcoRI–PstI AFLP primer combinations were applied to 121 distinct genotypes of mei cultivars and related species. A total of 508 AFLP
product bands were produced, of which 382 were polymorphic. The unweighted pair group method with arithmetic averages analysis
was carried out based on these AFLP markers. From this analysis, “Qugeng Mei,” “Yan Mei,” “Chaodou Mei,” and mei cultivars
were seen to share the same P. mume genetic stem. The AFLP data were able to clearly discriminate P. mume from other species in the genus Prunus, with P. armeniaca aligning as its closest related species. Two major groups and nine subgroups of mei flower were identified, and there was
a strong coincidence of these AFLP-based groupings with the respective morphological characters of the accessions. The genetic
diversity of mei accessions was greatest in the Yunnan Province and decreased toward Eastern China and Japan, so supporting
the hypothesis that the southwest of China represents the genetic diversity center of the species. 相似文献
13.
14.
Populations of the “continental” Great Cormorant P. c. sinensis have expanded from north-eastern Europe towards the western part of the range of the “marine” P. c. carbo breeding in the United Kingdom and France. The aim of the study was to test the hypothesis of ecological segregation between
subspecies by analysing the structuring of the European populations. Sequencing the mtDNA of 231 birds belonging to 20 colonies
revealed 38 haplotypes based on 25 polymorphic sites (5.76% sequence divergence). P. c. sinensis (“S”) was well confirmed, but usual P. c. carbo formed two coastal populations, the real P. c. carbo “C” mainly in the western part of the range (United Kingdom, coastal France), and also in Norway and Sardinia, and “N”, branched
to the Japanese Cormorant P. capillatus and probably isolated by glaciations, mainly present in the Nordic range (Norway, but also on the coasts from Sweden to Brittany),
we named P. c. norvegicus. In a variable position in the trees but close to C is a group of undetermined origin haplotypes, named U, also present in
both traditional ranges. The new tree-nesting colonies in Brittany are clearly a mixture of S and the two clades C and N previously
described as P. c. carbo, with a decreasing proportion of C + N between 1993 (67%), 1996 (60%) and 2002 (33%) for the pioneering Grand-Lieu colony. These results confirmed the current introgression
of continental populations in the western range, with probable hybridization. Although the subspecies can switch habitats
locally due to social behaviour and migrations, the ecological segregation between the two usual subspecies appears to be
largely confirmed in Europe. 相似文献
15.
Karine Louise dos Santos Nivaldo Peroni Raymond Paul Guries Rubens Onofre Nodari 《Economic botany》2009,63(2):204-214
Traditional Knowledge and Management of
Feijoa (Acca sellowiana)
in Southern Brazil. This paper investigates traditional knowledge of the use and management of Acca sellowiana in southern Brazil. Fifty-six informants from three rural communities were assigned to one of four subgroups (“maintainers,”
“managers,” “cultivators,” or “users”) based on their responses regarding management and use of A. sellowiana. Traditional knowledge related to use of this species is widespread among rural residents, but traditional knowledge related
to management is fragmented depending on whether one uses, manages, or cultivates the species. Knowledge held in rural communities
suggests that A. sellowiana could play an expanded role in local economies as well as biodiversity conservation. We suggest that participatory research
could stimulate greater local use as well as on-farm conservation of A. sellowiana. 相似文献
16.
A seaweed with the local name “endong” is a rare eucheumoid variety that has recently been farmed in Tawi-Tawi, southern Philippines.
Local farmers describe it as “eel-like” because of its long, slender and smooth thalli. Being somewhat similar in appearance
to the dominantly farmed seaweed “tambalang” (Kappaphycus alvarezii var. tambalangii), farmers mix their harvests of “endong” with those of “tambalang”. We analyzed the hydrocolloid extract from “endong” using
spectroscopic and chemical techniques. The extract’s infrared and nuclear magnetic resonance (1H and 13C) spectra, and sulfate and 3,6-anhydrogalactose contents revealed carrageenan of the iota-type, similar to extracts from
another farmed species, Eucheuma denticulatum. This result implies that “endong” carrageenan exhibits physico-chemical properties different from those of kappa-carrageenan
of “tambalang”. The findings of this study are of significance to the seaweed industry, as carrageenan quality problems could
potentially arise from a mixed harvest. Seaweed farmers are advised to separate their harvests of “endong” and “tambalang”.
Presented at the 19th International Seaweed Symposium, Kobe, Japan 相似文献
17.
C. A. Abella X. P. Cristina A. Martinez I. Pibernat X. Vila 《Archives of microbiology》1998,169(5):452-459
Two new phototrophic consortia, “Chlorochromatium lunatum” and “Pelochromatium selenoides”, were observed and collected in the hypolimnion of several dimictic lakes in Wisconsin and Michigan (USA). The two consortia
had the same morphology but different pigment composition. The cells of the photosynthetic components of the consortia were
half-moon-shaped. This morphology was used to differentiate them from the previously described motile phototrophic consortia
“Chlorochromatium aggregatum” and “Pelochromatium roseum”. These phototrophic cells did not resemble any described unicellular green sulfur bacteria. The predominant pigments detected
were bacteriochlorophyll d and chlorobactene for the green-colored “Clc. lunatum”, and bacteriochlorophyll e and isorenieratene for the brown-colored “Plc. selenoides”. Their pigment compositions and the presence of chlorosomes attached to the inner face of the cytoplasmic membrane in both
kinds of photosynthetic cells confirmed this new half-moon-shaped morphotype as a green sulfur bacterium. Both consortia were
found thriving in lakes with low concentrations of sulfide (< 60 μM), below the layers of “Clc. aggregatum” and “Plc. roseum”. The green consortia were observed in lakes where the oxic-anoxic interface was located at shallow depths (2–7 m), while
the brown consortia were found at greater depths (8–16 m). The two newly described consortia were never detected together
at the same depth in any lake.
Received: 30 April 1997 / Accepted: 17 January 1998 相似文献
18.
Abdel Gaffar Elhag Said Toshio Murashige 《In vitro cellular & developmental biology. Plant》1979,15(8):593-602
Summary Initial trials with tomato-root cultures disclosed the desirability of employing a gently agitated liquid medium containing
iron in the chelated form. For the normal cultivars “Ace” and “Tropic”, subcultures were best achieved by utilizing sectors
that possessed one or more newly emerged laterals. Continuous cultures of a nonlateral-forming tomato mutant, “Diageotropica”,
and of citron were accomplished by subculturing tips of the elongating primary roots. The tomato roots were cultured in White's
medium with the Fe2(SO4)3 replaced by 0.03 mM NaFeEDTA. Sustained growth of citron-root tips necessitated the use of a medium containing Murashige
and Skoog salts, 7.5% sucrose, 100 mg per I each of citric acid and thiamine HCl, and 5000 mg per li-inositol. The success with citron-root cultures was extendable to all cultivars ofC. medica L., but not to otherCitrus species relatives. Both citron and “Diageotropica” root cultures manifested undiminished elongation through repeated subcultures;
but neither produced laterals in response to any cultural treatments.
Research was supported in part by National Science Foundation Grant OIP75-10390 and Elvenia J. Slosson Fellowship in Ornamental
Horticulture. 相似文献
19.
Paula Sánchez-Hernández Martha P. Ramírez-Pinilla Miguel Molina-Borja 《Acta ethologica》2012,15(1):65-71
There have been relatively few attempts to quantitatively describe behaviours in scincid lizards. Chalcides viridanus is a small body-sized skink endemic of Tenerife (Canary Islands). We describe and quantify 18 behaviour patterns (both social
and agonistic) of this species, some of which have not been described before for other scincids. Video recordings of male–male,
female–female, and male–female interactions were made under laboratory conditions, with controlled light–dark cycle and temperature.
We describe several agonistic and courtship behaviour patterns. Within the first context, we detected a new agonistic behaviour
for a scincid, “Snout to body”, that appeared at the beginning of agonistic sequences; it consisted of each animal placing
its snout in contact with the other individual’s lateral side of the body. The amplitude of head movement during “Head bobbing”
was lower than that described for many other lizard species. Agonistic behaviours were shown in intrasexual staged encounters
both within males and females. The comparison of behaviour patterns of both types of intrasexual encounters showed that females
were more active, exhibiting significantly higher frequencies of behaviour than males. Specifically, females showed the “Snout
to body” pattern more frequently than males. In male–female encounters we detected courtship and copulation patterns only
in April, when males performed “Bites” and “Snout to body” directed at females. 相似文献
20.
Parent-specific, randomly amplified polymorphic DNA (RAPD) markers were obtained from total genomic DNA ofChlamydomonas reinhardtii. Such parent-specific RAPD bands (genomic fingerprints) segregated uniparentally (through mt+) in a cross between a pair of polymorphic interfertile strains ofChlamydomonas (C. reinhardtii andC. minnesotti), suggesting that they originated from the chloroplast genome. Southern analysis mapped the RAPD-markers to the chloroplast
genome. One of the RAPD-markers, “P2” (1.6 kb) was cloned, sequenced and was fine mapped to the 3 kb region encompassing 3′
end of 23S, full 5S and intergenic region between 5S and psbA. This region seems divergent enough between the two parents,
such that a specific PCR designed for a parental specific chloroplast sequence within this region, amplified a marker in that
parent only and not in the other, indicating the utility of RAPD-scan for locating the genomic regions of sequence divergence.
Remarkably, the RAPD-product, “P2” seems to have originated from a PCR-amplification of a much smaller (about 600 bp), but
highly repeat-rich (direct and inverted) domain of the 3 kb region in a manner that yielded no linear sequence alignment with
its own template sequence. The amplification yielded the same uniquely “sequence-scrambled” product, whether the template
used for PCR was total cellular DNA, chloroplast DNA or a plasmid clone DNA corresponding to that region. The PCR product,
a "unique" new sequence, had lost the repetitive organization of the template genome where it had originated from and perhaps
represented a “complex path” of copy-choice replication. 相似文献