共查询到20条相似文献，搜索用时 0 毫秒
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Yongjian Zhang Xiangyu Wu Lixing Yuan 《The Plant journal : for cell and molecular biology》2020,102(4):823-837
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Daisuke Iyaguchi Min Yao Isao Tanaka Eiko Toyota 《Acta Crystallographica. Section F, Structural Biology Communications》2009,65(3):285-287
Adenylate/uridylate‐rich elements (AREs), which are found in the 3′‐untranslated region (UTR) of many mRNAs, influence the stability of cytoplasmic mRNA. HuR (human antigen R) binds to AREs and regulates various genes. In order to reveal the RNA‐recognition mechanism of HuR protein, an RNA‐binding region of human HuR containing two N‐terminal RNA‐recognition motif domains bound to an 11‐base RNA fragment has been crystallized. The crystals belonged to space group P212121, with unit‐cell parameters a = 42.4, b = 44.9, c = 91.1 Å. X‐ray diffraction data were collected to 1.8 Å resolution. 相似文献
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Kristina Kapinas Catherine B. Kessler Anne M. Delany 《Journal of cellular biochemistry》2009,108(1):216-224
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Dev A Nayernia K Meins M Adham I Lacone F Engel W 《Molecular reproduction and development》2007,74(11):1456-1464
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Brittany R Morgan Francesca Massi 《Protein science : a publication of the Protein Society》2010,19(6):1222-1234
TIS11d is a member of the CCCH-type family of tandem zinc finger (TZF) proteins; the TZF domain of TIS11d (residues 151–220) is sufficient to bind and destabilize its target mRNAs with high specificity. In this study, the TZF domain of TIS11d is simulated in an aqueous environment in both the free and RNA-bound states. Multiple nanosecond timescale molecular dynamics trajectories of TIS11d wild-type and E157R/E195K mutant with different RNA sequences were performed to investigate the molecular basis for RNA binding specificities of this TZF domain. A variety of measures of the protein structure, fluctuations, and dynamics were used to analyze the trajectories. The results of this study support the following conclusions: (1) the structure of the two fingers is maintained in the free state but a global reorientation occurs to yield a more compact structure; (2) mutation of the glutamate residues at positions 157 and 195 to arginine and lysine, respectively, affects the RNA recognition by this TIS11d mutant in agreement with the findings of Pagano et al. (J Biol Chem 2007; 282:8883–8894); and (3) we predict that the E157R/E195K mutant will present a more relaxed RNA binding specificity relative to wild-type TIS11d based on the more favorable nonsequence-specific Coulomb interaction of the two positively charged residues at positions 157 and 195 with the RNA backbone, which compensates for a partial loss of the stacking interaction of aromatic side chains with the RNA bases. 相似文献
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