Estimating the Microtubule GTP Cap Size In Vivo

1. Download : Download high-res image (160KB)
2. Download : Download full-size image

Highlights? The microtubule (MT) GTP cap is ～750 tubulin subunits in vivo and extends over 1 μm ? EGFP brightness can be calibrated in vivo using GFP-tubulin signals from MTs ? The presence of GTP-tubulin in the MT lattice influences MT shortening and rescue ? MT tip trackers, like EB1, can be highly concentrated at the MT plus end (～100 μM)     

AMPK Is a Negative Regulator of the Warburg Effect and Suppresses Tumor Growth In Vivo

1. Download : Download high-res image (85KB)
2. Download : Download full-size image

Highlights? Loss of AMPKα1 cooperates with the Myc oncogene to accelerate lymphomagenesis ? AMPKα dysfunction enhances aerobic glycolysis (Warburg effect) ? Inhibiting HIF-1α reverses the metabolic effects of AMPKα loss ? HIF-1α mediates the growth advantage of tumors with reduced AMPK signaling     

In Vivo HIF-Mediated Reductive Carboxylation Is Regulated by Citrate Levels and Sensitizes VHL-Deficient Cells to Glutamine Deprivation

1. Download : Download high-res image (183KB)
2. Download : Download full-size image

Highlights? HIF is necessary and sufficient to induce reductive carboxylation in RCC cell lines ? HIF promotes the reductive carboxylation flux by reducing citrate levels ? HIF-expressing tumors display reductive carboxylation in vivo ? HIF renders RCC cells sensitive to glutaminase inhibition in vitro and in vivo     

Integration of Genome-wide Approaches Identifies lncRNAs of Adult Neural Stem Cells and Their Progeny In Vivo

1. Download : Download high-res image (314KB)
2. Download : Download full-size image

Highlights? RNA-seq, RNA Capture-Seq of SVZ-NSCs reveals unannotated lncRNAs and splice variants ? lncRNA loci have dynamic chromatin signatures, including bivalency in SVZ-NSCs ? Online resource facilitates discovery of lncRNAs involved in adult neurogenesis ? lncRNAs can regulate glial-neuronal lineage specification of SVZ-NSCs     

In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis

1. Download : Download full-size image

Highlights? In vivo SILAC-based quantitative phosphoproteomics of skin carcinogenesis ? Phosphoproteomics identifies distinct signatures in skin and tumor tissues ? Phosphoproteomics pinpoints differences between papilloma and SCC tissues ? Predicted PAK4-PKC/SRC network is highly deregulated in SCC, but not papilloma     

In Vivo Epigenomic Profiling of Germ Cells Reveals Germ Cell Molecular Signatures

1. Download : Download high-res image (85KB)
2. Download : Download full-size image

Highlights? Modified small-scale ChIP-seq method applicable to small number of cells ? Genome-wide maps of H3K4me3, H3K27me3, H3K27ac, and H2BK20ac of germ cells in vivo ? Identification of active and inactive regulatory elements in germ cells in vivo ? Germ cell H3K27me3 regions are enriched for retrotransposon repeats     

Enabled Negatively Regulates Diaphanous-Driven Actin Dynamics In Vitro and In Vivo

1. Download : Download high-res image (166KB)
2. Download : Download full-size image

     

Repression of the Long Noncoding RNA-LET by Histone Deacetylase 3 Contributes to Hypoxia-Mediated Metastasis

1. Download : Download high-res image (174KB)
2. Download : Download full-size image

Highlights? There is low expression of lncRNA-LET in various tumor tissues ? Hypoxia-induced low expression of lncRNA-LET occurred through histone deacetylase 3 ? The lncRNA-LET is bound to NF90, which increases NF90 degradation by the proteasome ? Low lncRNA-LET expression contributes to hypoxia-induced cell invasion     

Principles of E-Cadherin Supramolecular Organization In Vivo

1. Download : Download high-res image (205KB)
2. Download : Download full-size image

     

Monitoring Tumorigenesis and Senescence In Vivo with a p16INK4a-Luciferase Model

1. Download : Download high-res image (210KB)
2. Download : Download full-size image

Highlights? Activation of cellular senescence with aging does not predict cancer mortality ? p16^LUC detects emerging tumors in 14 genetically distinct tumor models ? p16^LUC provides a detection advantage over other in vivo cancer monitoring systems ? Both the tumor and stroma of emerging neoplasms express high levels of p16^INK4a     

Autologous iPSC-Based Vaccines Elicit Anti-tumor Responses In Vivo

1. Download : Download high-res image (185KB)
2. Download : Download full-size image

     

Mixed-Linkage Ubiquitin Chains Send Mixed Messages

1. Download : Download high-res image (304KB)
2. Download : Download full-size image

Highlights? K48 and K63 linkages in branched and unbranched tri-Ub retain their structural features ? Linkage-selective receptors recognize K48 and K63 linkages in the branched tri-Ub ? Linkage-specific deubiquitinases cleave their cognate Ub-Ub linkages in the mixed-linkage chains ? The 26S proteasome recognizes and processes branched K48- and K63-linked tri-Ub     

Distinct States of Methionyl-tRNA Synthetase Indicate Inhibitor Binding by Conformational Selection

1. Download : Download high-res image (270KB)
2. Download : Download full-size image

Highlights? Complexes of MetRS from T. brucei with its substrate, intermediate, and inhibitors ? In crystals, substrate-bound MetRS undergoes extensive changes with inhibitor binding ? The inhibitor-bound conformation is surprisingly similar to the ligand-free state ? This indicates inhibitor binding occurs via conformational selection not by induced fit     

Designed Hsp90 Heterodimers Reveal an Asymmetric ATPase-Driven Mechanism In Vivo

1. Download : Download high-res image (154KB)
2. Download : Download full-size image

     

Reconstitution of Microtubule Nucleation In Vitro Reveals Novel Roles for Mzt1

1. Download : Download high-res image (159KB)
2. Download : Download full-size image

     

Organism-Scale Modeling of Early Drosophila Patterning via Bone Morphogenetic Proteins

1. Download : Download high-res image (265KB)
2. Download : Download full-size image

Highlights? Capturing 3D spatiotemporal content of expression data increases model utility ? Embryo geometry shapes signaling; prepatterns compensate in misshapen embryos ? Cell-surface binding proteins compensate for weak ligand/inhibitor/receptor binding ? BMP-mediated patterning is “nearly” scale-invariant     

An EB1-Kinesin Complex Is Sufficient to Steer Microtubule Growth In Vitro

1. Download : Download high-res image (82KB)
2. Download : Download full-size image

     

Secreted Frizzled-Related Protein 3 Regulates Activity-Dependent Adult Hippocampal Neurogenesis

1. Download : Download high-res image (313KB)
2. Download : Download full-size image

Highlights? Wnt inhibitor sFRP3 exhibits activity-dependent expression in the adult hippocampus ? sFRP3 maintains quiescence of adult hippocampal radial glia-like neural stem cells ? sFRP3 inhibits maturation, dendritic development, and spinal formation of new neurons ? sFRP3 partially mediates activity-dependent adult hippocampal neurogenesis