PGC-1 Coactivators Regulate MITF and the Tanning Response

1. Download : Download high-res image (175KB)
2. Download : Download full-size image

Highlights? PGC-1 induces pigment formation in melanocytes ? PGC-1s activate expression of MITF ? α-MSH induces PGC-1s, which are required for induction of melanogenic genes ? eQTLs in human PGC-1β are associated with tanning ability and melanoma protection     

Fas/CD95-Induced Chemokines Can Serve as “Find-Me” Signals for Apoptotic Cells

1. Download : Download high-res image (126KB)
2. Download : Download full-size image

Highlights? Fas-induced apoptosis is associated with secretion of cytokines and chemokines ? Fas-driven MCP-1 and IL-8 were chemotactic for monocytes and neutrophils, respectively ? IL-8 and MCP-1 can act as “find-me” signals for apoptotic cells ? Apoptotic cells can actively communicate with cells of the immune system     

ACTL6a Enforces the Epidermal Progenitor State by Suppressing SWI/SNF-Dependent Induction of KLF4

1. Download : Download high-res image (200KB)
2. Download : Download full-size image

Highlights? ACTL6a is downregulated during epidermal differentiation ? ACTL6a loss in epidermis causes progenitor loss and terminal differentiation ? ACTL6a is required for repression of KLF4 and other epidermal differentiation genes ? ACTL6a prevents binding of the SWI/SNF complex to differentiation genes     

Human IL-34 and CSF-1 Establish Structurally Similar Extracellular Assemblies with Their Common Hematopoietic Receptor

1. Download : Download high-res image (402KB)
2. Download : Download full-size image

Highlights? Structural insights into the complete extracellular human IL-34:CSF-1R complex ? The C-terminal tail of hIL-34 is flexible and heavily O-linked glycosylated ? Inclusion of N-linked glycans drastically improves fitting of models to SAXS data ? Consensus principles for hCSF-1R activation by its two distinct cytokine ligands     

The Bcl6-SMRT/NCoR cistrome represses inflammation to attenuate atherosclerosis

1. Download : Download high-res image (171KB)
2. Download : Download full-size image

Highlights? Bcl6 is a critical antiatherogenic regulator ? Inhibition of Bcl6-SMRT/NCoR interactions induces atherogenic gene expression ? SMRT and NCoR cistromes each exceed 30,000 sites, with a nearly 50% overlap ? Bcl6 recruits SMRT and NCoR to NF-κB-driven inflammatory and tissue remodeling genes     

Homeodomain-interacting protein kinase regulates yorkie activity to promote tissue growth

1. Download : Download high-res image (130KB)
2. Download : Download full-size image

Highlights? Hipk induces tissues overgrowth and leads to activation of Yki target genes ? Hipk is required for activity of hyperactive Yki ? Hipk kinase function is essential to promote Yki target genes     

Genetic Control of Obesity and Gut Microbiota Composition in Response to High-Fat,High-Sucrose Diet in Mice

1. Download : Download high-res image (253KB)
2. Download : Download full-size image

Highlights? Detailed analysis of diet-induced obesity in more than 100 inbred mouse strains ? Identification of 11 genetic loci associated with obesity and dietary responsiveness ? Significant overlap between mouse loci with human GWAS loci for obesity ? Strain-specific shifts in gut microbiota composition in response to dietary intervention     

Direct recruitment of polycomb repressive complex 1 to chromatin by core binding transcription factors

1. Download : Download high-res image (106KB)
2. Download : Download full-size image

Highlights? PRC1 interacts with Runx1 and CBFβ ? PRC1 and Runx1/CBFβ colocalize on chromatin and coregulate a subset of genes ? PRC1 and Runx1/CBFβ deficiencies generate partial phenocopies of one another in vivo ? Runx1 directly recruits PRC1-independent of PRC2     

Origin of immunoglobulin isotype switching

1. Download : Download high-res image (158KB)
2. Download : Download full-size image

Highlights? Switching in sharks consistent with immunization and cytidine deaminase expression ? Isotype switching can be reciprocal between genes of established positions ? Switch joints show DNA double-strand breakage repaired by nonhomologous end-joining ? Unlike in tetrapods, switching in shark is bidirection between IgM and IgW     

Dynamics of 5-Hydroxymethylcytosine and Chromatin Marks in Mammalian Neurogenesis

1. Download : Download full-size image

Highlights? 5hmC levels increase in neuronal function-related genes during neurogenesis ? 5hmC gain is accompanied by H3K27me3 loss at promoters and gene bodies ? Gain of 5hmC is not associated with substantial DNA demethylation ? Polycomb and Tet protein together promote proper progression of neurogenesis     

Riboswitch Control of Aminoglycoside Antibiotic Resistance

1. Download : Download high-res image (257KB)
2. Download : Download full-size image

Highlights? The 5′ leader RNA of aminoglycoside antibiotic-resistance genes is conserved ? Aminoglycosides induce reporter gene expression by interacting with the leader RNA ? The aminoglycosides bind to the leader RNA and induce a change in the RNA structure ? Induction is independent of leader peptide stalling or drug-ribosome interactions     

LRP6 Enhances Glucose Metabolism by Promoting TCF7L2-Dependent Insulin Receptor Expression and IGF Receptor Stabilization in Humans

1. Download : Download high-res image (198KB)
2. Download : Download full-size image

Highlights? Nondiabetic LRP6 mutation carriers are hyperinsulinemic and insulin resistant ? IR expression is reduced in skeletal muscles of the LRP6 mutation carriers ? Wnt/LRP6 regulate the insulin receptor and IGFR expression ? The LRP6 mutation reduces TCF7L2-dependent IR expression and enhances mTOR activity     

Design of a Phosphorylatable PDZ Domain with Peptide-Specific Affinity Changes

1. Download : Download high-res image (234KB)
2. Download : Download full-size image

Highlights? Successful design of phosphorylation sites into a globular protein ? Redesigned PDZ domain phosphorylation is associated with local destabilization ? Phosphorylation switches peptide binding function ? Phosphorylation switches peptide recognition specificity     

Sustained PU.1 Levels Balance Cell-Cycle Regulators to Prevent Exhaustion of Adult Hematopoietic Stem Cells

1. Download : Download high-res image (312KB)
2. Download : Download full-size image

Highlights? PU.1 prevents hematopoietic stem cell (HSC) exhaustion ? PU.1 is a master regulator of cell-cycle genes in HSCs ? PU.1 binding mediates chromosome looping in HSCs ? Positive autoregulation via a ?14 kb enhancer sustains PU.1 levels in HSCs     

O-GlcNAc Signaling Entrains the Circadian Clock by Inhibiting BMAL1/CLOCK Ubiquitination

1. Download : Download high-res image (176KB)
2. Download : Download full-size image

Highlights? The hexosamine/O-GlcNAc pathway regulates cellular clock oscillation ? OGT promotes expression of BMAL1/CLOCK target genes in cultured cells and liver ? Rhythmic O-GlcNAcylation stabilizes BMAL1 and CLOCK by inhibiting their ubiquitination ? Hepatic manipulation of OGT perturbs the diurnal rhythm of glucose homeostasis     

The RNA Pol II Elongation Factor Ell3 Marks Enhancers in ES Cells and Primes Future Gene Activation

1. Download : Download high-res image (254KB)
2. Download : Download full-size image

Highlights? Ell3 is preferentially found at active, poised, and inactive enhancers in ES cells ? Promoter-proximal Pol II occupancy at key developmental genes requires Ell3 ? Cohesin functions in Ell3-associated enhancer-promoter communication ? Recruitment of P-TEFb within SEC during differentiation is Ell3 dependent