The metabolic profile of tumors depends on both the responsible genetic lesion and tissue type

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Highlights? MYC and MET affect glucose and glutamine metabolism differently in the liver ? Tissue context affects the outcome of metabolic reprogramming by MYC ? MYC overexpression sensitizes cells to inhibition of Gls1 glutaminase     

RIF1 Is Essential for 53BP1-Dependent Nonhomologous End Joining and Suppression of DNA Double-Strand Break Resection

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Highlights? RIF1 is essential for 53BP1-dependent CSR and fusion of dysfunctional telomeres ? BRCA1 antagonizes RIF1 in S phase to prevent error-prone repair by toxic NHEJ ? N-terminal phospho-SQ/TQ domain of 53BP1 interacts with and recruits RIF1 to DSBs ? RIF1 and 53BP1 promote NHEJ in G1 by blocking 5′ end resection of DSBs     

Replacement of Oct4 by Tet1 during iPSC Induction Reveals an Important Role of DNA Methylation and Hydroxymethylation in Reprogramming

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Highlights? Tet1 facilitates iPSC induction by promoting Oct4 demethylation and reactivation ? Tet1 can replace Oct4 to generate fully pluripotent TSKM iPSCs ? Both 5mC and 5hmC increase on the genome during TSKM 2° reprogramming ? 5hmC promotes the demethylation of pluripotency genes during reprogramming     

Macrohistone Variants Preserve Cell Identity by Preventing the Gain of H3K4me2 during Reprogramming to Pluripotency

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Highlights? Macrohistone variants are regulated during reprogramming and differentiation ? The levels of macrohistone variants modulate the efficiency of reprogramming ? MacroH2A.1 occupies pluripotency and differentiation genes in keratinocytes ? MacroH2A.1 occupancy prevents the gain of H3K4me2 during reprogramming     

ESCs Require PRC2 to Direct the Successful Reprogramming of Differentiated Cells toward Pluripotency

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Highlights? Mouse pluripotent cells reprogram human B cells in heterokaryons ? ESCs lacking PRC1 or PRC2 cannot successfully reprogram ? Failure of PRC1/2-deficient ESCs to reprogram is functionally dominant ? PRC1/2 play a critical role in the chromatin reorganization required for reprogramming     

Streamlining genomes: toward the generation of simplified and stabilized microbial systems

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Highlights? Top-down and bottom-up approaches to genome streamlining. ? Computational support for constructing and refactoring streamlined genomes. ? From genome engineering to metabolic reprogramming. ? Perspectives in applied genome engineering.     

Protein Determinants of Meiotic DNA Break Hot Spots

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Highlights? DSB hot spots for meiotic recombination are specified by largely unknown mechanisms ? Linear element proteins, required for most DSBs, are highly enriched at hot spots ? These proteins, the first known global hot spot determinants, bind without DSB formation ? Hot spot determination by these proteins provides a paradigm for crossover control     

Different Roles for Tet1 and Tet2 Proteins in Reprogramming-Mediated Erasure of Imprints Induced by EGC Fusion

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Highlights? EGCs can erase DNA methylation at ICRs in somatic cells after fusion ? EGCs selectively induce 5hmC accumulation at ICRs in the somatic genome ? Conversion of 5mC to 5hmC at these imprinted domains requires Tet1 ? Tet2 depletion results in delayed reprogramming by EGCs     

Structure of a bacteriophytochrome and light-stimulated protomer swapping with a gene repressor

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Highlights? Bacteriophytochrome structure containing photosensory and output transducing domain ? RpBphP1 binds to the cognate repressor RpPpsR2 on illumination with far-red light ? HOS domain is distantly related to Dhp and is essential for binding to the repressor ? Light-induced protomer swapping mechanism between dimers     

The ROSA26-iPSC Mouse: A Conditional,Inducible, and Exchangeable Resource for Studying Cellular (De)Differentiation

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Highlights? Conditional and inducible reprogrammable mouse model presented ? Allows removal of the Yamanaka factors after reprogramming ? Fully and partially reprogrammed endothelial cells generated ? Lineage-directed differentiation of iPSCs toward beating cardiomyocytes     

Architecture of a dodecameric bacterial replicative helicase

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Highlights? Crystal structure of the ATPase domain of DnaB from Helicobacter pylori ? MALLS analysis shows that HpDnaB forms dodecamers ? EM reconstruction shows that HpDnaB forms head-to-head double hexamers     

Sprouty1 Regulates Reversible Quiescence of a Self-Renewing Adult Muscle Stem Cell Pool during Regeneration

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Highlights? Pax7⁺ skeletal satellite cells regenerate muscle in their endogenous environment ? Quiescent satellite cells express the RTK inhibitor Spry1 ? Spry1 is needed for satellite cells to return to quiescence after muscle injury ? Deletion of Spry1 in satellite cells depletes the muscle stem cell pool     

The bacterial stressosome: a modular system that has been adapted to control secondary messenger signaling

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Highlights? Regulation of cyclic di-GMP biosynthesis in Moorella thermoacetica ? Stressosome signal transduction and resetting ? X-ray crystal structure of type 2C phosphatase MtX ? X-ray crystal structures of phosphorylated MtS and the N-terminal domain of MtR     

Characterization and In Vivo Pharmacological Rescue of a Wnt2-Gata6 Pathway Required for Cardiac Inflow Tract Development

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Highlights? Wnt2 is required for atrial and inflow tract morphogenesis ? Wnt2 regulates expansion of secondary heart field progenitors ? Defects in Wnt2^?/? mutants can be rescued using Wnt signaling agonists ? Wnt2 cooperates with Gata6 to regulate cardiac inflow tract development     

Variation in the Dorsal Gradient Distribution Is a Source for Modified Scaling of Germ Layers in Drosophila

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Highlights? The germ layers of Drosophila species are unequally scaled to size during evolution ? Evolutionary changes in nuclear size and density affect the Dl gradient scaling ? Mesodermal variations rely on Dl distribution instead of regulation of target genes ? Dl gradient distortions allow new positioning of ventral neuroectodermal border     

MicroRNA-92a Upholds Bmp Signaling by Targeting noggin3 during Pharyngeal Cartilage Formation

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Highlights? mir92a inactivation leads to loss of pharyngeal cartilages in zebrafish embryos ? mir92a is required for chondrogenic progenitor proliferation and survival ? Bmp signaling is positively regulated by mir92a in the pharyngeal region ? nog3 mRNA is degraded by mir92a to maintain Bmp activity     

Genetic Correction of a LRRK2 Mutation in Human iPSCs Links Parkinsonian Neurodegeneration to ERK-Dependent Changes in Gene Expression

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Highlights? hiPSC-derived neurons with LRRK2 G2019S exhibit Parkinsonian neurodegeneration ? Genetic correction of the LRRK2 mutation rescued neurodegenerative phenotypes ? Comparative analysis links LRRK2 G2019S, gene dysregulation, and ERK activation ? ERK-dependent gene dysregulation by LRRK2 G2019S contributes to neurodegeneration