Snx3 Regulates Recycling of the Transferrin Receptor and Iron Assimilation

1. Download : Download high-res image (201KB)
2. Download : Download full-size image

Highlights? Snx3 is highly expressed in vertebrate hematopoietic tissues ? Silencing of Snx3 results in anemia and hemoglobin defects in vertebrates ? Snx3 and Vps35 physically interact with Tfrc ? Snx3 is required for endosomal recycling of Tf-Tfrc complex     

LRP6 Enhances Glucose Metabolism by Promoting TCF7L2-Dependent Insulin Receptor Expression and IGF Receptor Stabilization in Humans

1. Download : Download high-res image (198KB)
2. Download : Download full-size image

Highlights? Nondiabetic LRP6 mutation carriers are hyperinsulinemic and insulin resistant ? IR expression is reduced in skeletal muscles of the LRP6 mutation carriers ? Wnt/LRP6 regulate the insulin receptor and IGFR expression ? The LRP6 mutation reduces TCF7L2-dependent IR expression and enhances mTOR activity     

Anions Mediate Ligand Binding in Adineta vaga Glutamate Receptor Ion Channels

1. Download : Download high-res image (223KB)
2. Download : Download full-size image

Highlights? The AvGluR1 LBD is a hybrid structure with iGluR prokaryotic and eukaryotic features ? AvGluR1 binds hydrophobic amino acids as well as glutamate ? Chloride ions acts as surrogate ligand atoms in the Ala, Ser, and Met complexes ? The AvGluR1 LBD assembles as a dimer but with very low affinity     

RANKL Employs Distinct Binding Modes to Engage RANK and the Osteoprotegerin Decoy Receptor

1. Download : Download high-res image (257KB)
2. Download : Download full-size image

Highlights? Crystal structures of the cytokine-binding regions of OPG and RANK bound to RANKL ? High-affinity OPG engagement is associated with a distinct RANKL-binding cleft ? Structural basis of how the OPG^F96L mutation promotes juvenile Paget’s disease     

Endogenous Purification Reveals GREB1 as a Key Estrogen Receptor Regulatory Factor

1. Download : Download full-size image

Highlights? A proteomic method identifies protein-protein interaction in primary tumors ? GREB1 is the top estrogen-induced ER-interacting protein ? GREB1 is an essential ER cofactor recruited to chromatin ? GREB1 is an independent prognostic marker     

Human IL-34 and CSF-1 Establish Structurally Similar Extracellular Assemblies with Their Common Hematopoietic Receptor

1. Download : Download high-res image (402KB)
2. Download : Download full-size image

Highlights? Structural insights into the complete extracellular human IL-34:CSF-1R complex ? The C-terminal tail of hIL-34 is flexible and heavily O-linked glycosylated ? Inclusion of N-linked glycans drastically improves fitting of models to SAXS data ? Consensus principles for hCSF-1R activation by its two distinct cytokine ligands     

Functional Insights from the Crystal Structure of the N-Terminal Domain of the Prototypical Toll Receptor

1. Download : Download high-res image (219KB)
2. Download : Download full-size image

Highlights? The N-terminal cap of Toll adopts a new fold ? It resembles a duplicated beta hairpin structure with three disulphide bonds ? Toll LRRNT cap is not critical for Spätzle binding ? The first 13 LRRs are sufficient for Spätzle binding     

Insights into Negative Regulation by the Glucocorticoid Receptor from Genome-wide Profiling of Inflammatory Cistromes

1. Download : Download high-res image (136KB)
2. Download : Download full-size image

Highlights? TLR4 activation dramatically remodels the GR cistrome in macrophages ? Both negative and positive GR enhancers include GREs, NF-κB, and AP-1 sites ? Classical models fail to predict positive or negative polarity of GR regulation ? Hormone treatment marks negative enhancers by histone deacetylation     

Dynamic Interaction between Sigma-1 Receptor and Kv1.2 Shapes Neuronal and Behavioral Responses to Cocaine

1. Download : Download high-res image (433KB)
2. Download : Download full-size image

Highlights? Sig-1R activity enhances sensitivity to cocaine by decreasing accumbal firing ? Cocaine decreases accumbal firing via Sig-1R-dependent increase of Kv1.2 current ? The Sig-1R forms a complex with Kv1.2 channel in the nucleus accumbens shell ? Cocaine triggers a long-lasting upregulation of the Sig-1R-Kv1.2 channel complex     

Bystander-Activated Memory CD8 T Cells Control Early Pathogen Load in an Innate-like,NKG2D-Dependent Manner

1. Download : Download full-size image

Highlights? Memory CD8 T cells become cytotoxic when activated by inflammation (BA-CTLs) ? Bystander activation of memory CD8 T cells occurs with minimal TCR signaling ? BA-CTLs eliminate target cells in an innate-like, NKG2D-dependent manner ? BA-CTLs are necessary to limit pathogen replication early after an infection     

Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayers

1. Download : Download high-res image (125KB)
2. Download : Download full-size image

Highlights? RNAi screens reveal 117 host molecules involved in enterovirus infection ? Components of immune signaling including MAPKs, Akts, and TLR8 restrict infection ? Adenylate cyclases are required for both PV and CVB infection ? Rab GTPases, Src tyrosine kinases, and tyrosine phosphatases control infection     

Inflammatory Chemokines Direct and Restrict Leukocyte Migration within Live Tissues as Glycan-Bound Gradients

1. Download : Download high-res image (352KB)
2. Download : Download full-size image

Highlights? Chemokines form tissue-bound gradients in vivo through heparan sulfate proteoglycans ? Chemokines guide neutrophils in vivo as haptotactic gradients ? Chemokines guide neutrophils toward infection loci by triggering orthotaxis ? Chemokines locally restrict neutrophil motility at sites of infection     

Moderation of Arabidopsis Root Stemness by CLAVATA1 and ARABIDOPSIS CRINKLY4 Receptor Kinase Complexes

1. Download : Download high-res image (385KB)
2. Download : Download full-size image

Highlights? CLV1 receptor kinase regulates stemness in Arabidopsis root and shoot meristems ? CLV1 and ACR4 form homo- and heteromeric complexes as shown by a novel FRET assay ? CLV1/ACR4 complexes differ in composition between plasma membrane and plasmodesmata ? CLV1/ACR4 complexes regulate distal root meristem maintenance     

Monitoring the inflammatory response to infection through the integration of MALDI IMS and MRI

1. Download : Download high-res image (103KB)
2. Download : Download full-size image

Highlights? IMS provides a whole-animal view of the inflammatory response to infection ? IMS identifies protein masses that are abundant at sites of inflammation ? Anatomic information at the site of infection can be generated by MRI ? Integrated IMS and MRI enable a 3D view of the host-pathogen interaction     

The Ciliary G-Protein-Coupled Receptor Gpr161 Negatively Regulates the Sonic Hedgehog Pathway via cAMP Signaling

1. Download : Download high-res image (363KB)
2. Download : Download full-size image

Highlights? The GPCR Gpr161 localizes to primary cilia in a Tulp3/IFT-A-dependent manner ? Complete loss of Gpr161 increases Shh signaling in the mouse neural tube ? Gpr161 couples protein kinase A activation to Shh signaling via cAMP signaling ? Shh signaling internalizes Gpr161 from the cilia, preventing its activity     

An ERK-p38 Subnetwork Coordinates Host Cell Apoptosis and Necrosis during Coxsackievirus B3 Infection

1. Download : Download high-res image (237KB)
2. Download : Download full-size image

Highlights? Intracellular signaling predicts the fate of coxsackievirus B3 (CVB3)-infected cells ? p38 and ERK signaling pathways are tightly interconnected during CVB3 infection ? CVB3-induced apoptosis of cardiomyocytes is inhibited by ERK5 and ERK1/2 pathways ? CVB3 induces necrosis of cardiomyocytes via p38 activation     

Class II Major Histocompatibility Complex Plays an Essential Role in Obesity-Induced Adipose Inflammation

1. Download : Download high-res image (274KB)
2. Download : Download full-size image

Highlights? Obesity enhances MHCII expression in primary human and mouse adipocytes ? Adipocytes activate CD4+ ARTs via MHCII and leptin to induce adipose inflammation ? Macrophage changes in adipose follow adipocyte and T cell interactions during HFD ? Adaptive immune mechanisms are essential to obesity-induced adipose inflammation     

c-MYC-Induced Sebaceous Gland Differentiation Is Controlled by an Androgen Receptor/p53 Axis

1. Download : Download full-size image

Highlights? Myc-induced sebaceous differentiation depends on the androgen receptor and p53 ? The androgen receptor promotes sebocyte differentiation ? Myc induces a p53 response that promotes sebocyte proliferation ? p53 and the androgen receptor are mutually antagonistic     

Incoming RNA Virus Nucleocapsids Containing a 5′-Triphosphorylated Genome Activate RIG-I and Antiviral Signaling

1. Download : Download high-res image (187KB)
2. Download : Download full-size image

Highlights? RIG-I is activated by the incoming RNA virus nucleocapsids during infection ? RIG-I activation requires a 5′triphosphate dsRNA structure on the nucleocapsids ? Viral nucleocapsids trigger conformational switching and oligomerization of RIG-I ? RIG-I directly binds to viral nucleocapsids containing a 5′triphosphate dsRNA structure