Resistance to eyespot of wheat, caused by Tapesia yallundae, derived from Thinopyrum intermedium homoeologous group 4 chromosome

Thinopyrum intermedium was identified previously as resistant to Tapesia yallundae, cause of eyespot of wheat. Using GUS-transformed isolates of T. yallundae as inoculum, we determined that wheat lines carrying Th. intermedium chromosome 4Ai#2 or the short arm of chromosome 4Ai#2 were as resistant to the pathogen as the eyespot-resistant wheat- Th. ponticum chromosome substitution line SS767 (PI 611939) and winter wheat cultivar Madsen, which carries gene Pch1 for eyespot resistance. Chromosome 4E from Th. elongatum and chromosome 4J from Th. bessarabicum did not confer resistance to T. yallundae. Genome-specific PCR primers confirmed the presence of Thinopyrum chromatin in these wheat- Thinopyrum lines. Genomic in situ hybridization using an St genomic probe from Pseudoroegneria strigosa demonstrated that chromosome 4Ai#2 belongs to the J^s genome of Thinopyrum. The eyespot resistance in the wheat- Th. intermedium lines is thus controlled by the short arm of this J^s chromosome. This is the first report of resistance to T. yallundae controlled by a J^s genome chromosome of Th. intermedium.     

Long-term performance of fungicides applied to control eyespot in winter wheat

Fungicide treatments, repeated twice yearly at timings suitable for controlling eyespot, to plots of continuous winter wheat over 17 yr (except for a year in “set‐aside”), resulted in altered population structures of the eyespot pathogens (Tapesia spp.) and performance of the fungicides. Carbendazim failed to control eyespot after 3 yr of the experiment because of rapid selection for resistance. Carbendazim often increased disease compared with that in untreated plots. This may have resulted from control of unknown antagonistic fungi or, possibly, from selection for increased fitness in carbendazim ‐resistant strains. In the final years of the experiment, prochloraz, applied with or without carbendazim, became ineffective. This gradual loss of efficacy is explained by selection in favour of T. acuformis rather than T. yallundae (which had a naturally narrower range of sensitivity levels in its populations) and for strains with least sensitivity within the T. acuformis population, although no resistance to prochloraz was found. Effects of treatments on yield were usually not statistically significant. This may be explained partly by the predominance of the more slowly developing T. acuformis in many of the plots.     

Development of Eyespot and Brown Foot Rot in Wheat Plants Inoculated with Mixtures of Pseudocercosporella herpotrichoides Types and Fusarium Species

The effects of Fusarium avenaceum, F. culmorum and Microdochium nivale on eyespot development and of types of the eyespot fungus Pseudocercosporella herpotrichoides on brown foot rot caused by the Fusarium or Microdochium spp. were investigated by sequential inoculation of wheat plants grown in pots in a controlled environment. The W-type of the eyespot fungus induced more severe disease than the R-type, but its symptons were suppressed to a greater extent by Fusarium spp., especially F. avenaceum. Brown foot rot symptoms were sometimes suppressed by P. herpotrichoides but were occasionally more severe when the Fusarium inoculum was applied after the P. herpotrichoides. There, sults are discussed in relation to observations of natural infections.     

Surveys of stem base diseases and fusarium ear diseases in winter wheat in England, Wales and Scotland, 1989–1990

An investigation into the incidence of stem base diseases and the pathogens associated with them was undertaken on winter wheat samples collected at growth stages 31 and 73–75 in 1989 and 1990. Symptoms at growth stage 31 were classified into 10 different types based on visual characteristics. Although Pseudocercosporella herpotrichoides was associated mainly with eyespot lesions and Rhizoctonia spp. with sharp eyespot lesions, a significant number of isolates of each pathogen were taken from lesions classified as fusarium. Different types of fusarium lesion on stem bases at both growth stages were not associated with any one particular Fusarium or Microdochium species. 98% of P. herpotrichoides isolates obtained from eyespot lesions at growth stage 31 in 1989, and 87% of those obtained in 1990 were identified as the sub-species P. herpotrichoides var. acuformis, Microdochium nivale was more common than any of the Fusarium species at both growth stages, and was particularly prevalent in samples from Scotland at the earlier growth stage in 1989. Isolations from the top internodes at growth stage 73–75 indicated that systemic infection by Fusarium species, although present in some stems, was of little importance. Fusarium ear blight affected 0.4% of ears in 1989 and 0.5% in 1990. Glume spot lesions on the ears in 1990 were predominantly associated with Fusarium poae.     

Strategies for control of eyespot (Pseudocercosporella herpotrichoides) in UK winter wheat and winter barley

This review considers strategies for control of eyespot (Pseudocercosporella herpotrichoides) in winter wheat and winter barley in the UK. Varietal resistance to eyespot may take the form of direct resistance to the growth of the pathogen in the stem base or of indirect tolerance to eyespot through resistance to lodging. The French variety Cappelle-Desprez has been a source of resistance for most UK wheat varieties and a new source of resistance in wild goat grass has been discovered recently. Use of fungicides for control of eyespot increased rapidly after the introduction of the highly effective MBC fungicides in the 1970s, but has decreased recently because UK populations of P. herpotrichoides are now predominantly resistant to MBC fungicides and alternative fungicides are more expensive. Historically, cultural methods, especially crop rotation, have been important in the control of eyespot. The importance of cultural control of eyespot is now increasing because more non-cereal break crops are being grown and fungicide use is declining.     

Identification of a QTL conferring seedling and adult plant resistance to eyespot on chromosome 5A of Cappelle Desprez

Eyespot is an economically important fungal disease of wheat and other cereals caused by two fungal species: Oculimacula yallundae and Oculimacula acuformis. However, only two eyespot resistance genes have been characterised and molecular markers made available to plant breeders. These resistances are Pch1, introduced into wheat from the relative Aegilops ventricosa, and Pch2, originally identified in the cultivar Cappelle Desprez (CD). There are drawbacks associated with both resistances; Pch1 is linked to deleterious traits carried on the Ae. ventricosa introgression and Pch2 has been shown to have limited effectiveness. An additional resistance has been reported on chromosome 5A of CD that confers resistance to eyespot in adult plants. In the present study, we demonstrate that resistance on this chromosome is effective against both O. yallundae and O. acuformis eyespot pathogens and confers resistance at both seedling and adult plant stages. This resistance was mapped in both seedling bioassays and field trials in a 5A recombinant population derived from a cross between CD and a CD single chromosome substitution line carrying 5A from the susceptible line Bezostaya. The resistance was also mapped using seedling bioassays in a 5A recombinant population derived from a cross between the susceptible line Chinese Spring (CS) and a single chromosome substitution line carrying 5A from CD. A single major QTL on the long arm of chromosome 5A was detected in all experiments. Furthermore, the SSR marker Xgwm639 was found to be closely associated with the resistance and could be used for marker-assisted selection of the eyespot resistance by plant breeders.     

Surveys of diseases of winter wheat in England and Wales, 1976–1988

Samples from 300 – 400 randomly selected winter wheat crops were taken annually at growth stage 73 – 75 from 1976 to 1988 with the exception of 1983 and 1984. The number of samples from each region was proportional to the area of wheat grown in each region. The percentage of the area of the top two leaves affected by diseases, the severity of ear and stem base diseases and, in 6 years, the severity of take-all were recorded. Septoria tritici and Septoria nodorum were, on average, the most severe of the foliar diseases and eyespot (Pseudocercosporella herpotrichoides) was the most severe of the stem base diseases. Regional differences in levels of S. tritici, brown rust, sharp eyespot and nodal fusarium were significant. Cultivar resistance affected disease severity, and previous cropping patterns particularly affected take-all and eyespot. Eyespot and sharp eyespot were less severe in late- than in early-sown crops. The percentage of crops treated with a fungicidal spray increased from 14% in 1976 to over 90% between 1983 and 1985. Use of benzimidazole fungicides applied at growth stage 31 declined, while use of morpholines from flag leaf emergence onwards increased between 1985 and 1988.     

Microbiota in Wheat Roots,Rhizosphere and Soil in Crops Grown in Organic and Other Production Systems

Culturable microbial communities and diseases were compared in organic, integrated and conventional systems of winter wheat production and monoculture. Particular emphasis was placed on the density and diversity of cereal pathogens and their potential antagonists, and on the association of the active microbial populations with the health and productivity of wheat. In roots, rhizoplane and rhizosphere, fungi tended to be most abundant in the integrated system or monoculture, and bacteria in the organic system. The dominant fungal groups (with individual frequency >5%) included root pathogens (Fusarium, Gibberella, Haematonectria and Ilyonectria) and known pathogen antagonists (Acremonium strictum, Clonostachys, Chaetomium, Gliocladium and Trichoderma spp.). The 50 subdominant species (with individual frequency 1–5%) included the pathogens Alternaria, Cladosporium (leaf spot), Gaeumannomyces graminis (take‐all), Glomerella graminicola (anthracnose), Oculimacula yallundae (eyespot), Phoma spp. (leaf spots), and Pythium and Rhizoctonia (root rot). The 40 subrecedent species (with individual frequency <1%) included minor pathogens (Botrytis, Coniothyrium, Leptosphaeria). Antagonists in roots, rhizoplane and rhizosphere were most frequent in the organic system and least frequent in monoculture, suggesting that these systems had the most and least disease‐suppressive habitats, respectively. The other two systems were intermediate, with microbial communities suggesting that the conventional system produced a slightly more suppressive environment than the integrated system. The highest grain yield, in the integrated system, was associated with high abundance of fungi, including fungal pathogens, lowest abundance of Arthrobacter, Pseudomonas and Streptomyces in roots, rhizoplane and soil, and relatively high stem‐base and leaf disease severity. The lowest grain yields, in the organic system and monoculture, were associated with less abundant fungi and more abundant Pseudomonas. There is no clear indication that yields were affected by diseases.     

Surveys of diseases of winter barley in England and Wales, 1981–1991

Samples from 200–400 randomly selected winter barley crops were taken annually at growth stage 71–73 from 1981 to 1991, with the exception of 1984 and 1985. The number of samples from each region was proportional to the area of barley growth in each region. The percentage of the area of the top two leaves affected by diseases and the severity of stem base diseases were recorded. Mildew (Erysiphe graminis) was the most widespread of the foliar diseases and in three years (1982, 1986 and 1991) was also the most severe. Rhynchosporium (Rhynchosporium secalis), net blotch (Pyrenophora teres) and brown rust (Puc-cinia hordei) were also prevalent in some years. Of the stem base diseases, fusarium was often the most widespread. Eyespot (Pseudocercosporella her-potrichoides) severity varied widely from year to year ranging from 1.2% of stems affected by moderate or severe symptoms in 1982 to 24.1% in 1988. There were regional differences in the severity of mildew, rhynchosporium, brown rust, halo spot (Selenophoma donacis) and eyespot. Cultivar resistance affected disease severity and previous cropping affected eyespot and less frequently mildew, rhynchosporium and net blotch. Eyespot, and to a lesser extent, sharp eyespot, were less severe in late- than in early-sown crops. The percentage of crops treated with a fungicidal spray increased from 72% in 1981 to 95% in 1991. The use of benzimidazole fungicides for the control of eyespot declined in response to the development of resistance, and more recently the use of prochlo-raz also declined. Broad spectrum DMI fungicides were widely used, and the use of morpholines to improve mildew control increased significantly. The proportion of crops grown from seed treated with a non-mercurial fungicidal seed dressing reached a peak of 47% in 1986 but subsequently declined to 22% in 1990 and 1991.     

Mapping resistance genes for Oculimacula acuformis in Aegilops longissima

Key message

This study identified three QTL conferring resistance to Oculimacula acuformis in Aegilops longissima and their associated markers, which can be useful in marker-assisted selection breeding for eyespot resistance.

Abstract

Oculimacula acuformis is one of two species of soilborne fungi that cause eyespot of wheat, the other being Oculimacula yallundae. Both pathogens can coexist in the same field and produce elliptical lesions on stem bases of wheat that are indistinguishable. Pch1 and Pch2 are the only two eyespot resistance genes readily available to wheat breeders, but neither provides complete control. A new source of eyespot resistance was identified from Aegilops longissima (2n = 14, S^lS^l), a wild relative of wheat. Three QTL for resistance to O. acuformis were mapped in chromosomes 1S^l, 3S^l, and 5S^l using a recombinant inbred line population developed from the cross Ae. longissima accessions PI 542196 (R) × PI 330486 (S). The three QTL explained 66 % of phenotypic variation by β-glucuronidase score (GUS) and 84 % by visual rating. These QTL had LOD values of 10.6, 8.8, and 6.0 for GUS score, and 16.0, 10.0, and 13.0 for visual rating. QTL associated with resistance to O. acuformis have similar chromosomal locations as some for resistance to O. yallundae, except that a QTL for resistance to O. yallundae was found in chromosome 7S^l but not for O. acuformis. Thus, it appears that some genes at the same locus in Ae. longissima may control resistance to both eyespot pathogens. QTL effective against both pathogens will be most useful for breeding programs and have potential to improve the effectiveness and genetic diversity of eyespot resistance.     

Analysis of Microdochium nivale isolates from wheat in the UK during 1993

A total of 144 isolates of Microdochium nivale from stem bases of winter wheat were taken from 30 sites and 91 isolates from grain were taken from seven sites throughout the UK. Identification by polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) region followed by restriction enzyme digestion of the PCR product revealed that 70% of stem base isolates were M. nivale var. majus and 30% var. nivale while 93% grain isolates were var. majus and 7% var. nivale. Almost all isolates were resistant to the benzimidazole fungicide benomyl. Perithecial production in vitro was more common in var. majus isolates and occurred in almost all grain isolates, but was less common in stem base isolates. The implications of the findings in terms of epidemiology and chemical control of this important cereal pathogen are discussed.     

Effects of treating seed potatoes from commercial and stem cutting stocks with benomyl, thiabendazole and 2-aminobutane on yield and disease

Potato seed tubers of six cultivars from commercial stocks and from stocks derived from stem cuttings (healthier seed) were fumigated with 2-aminobutane 2 wk after lifting or treated with benomyl or thiabendazole in January. 2-aminobutane prevented skin spot and gangrene developing on treated tubers. Experiments were planted at Rothamsted (clay with flints soil) and at Woburn (sandy loam soil) in 1973–75. Healthier seed produced more stems/plant than commercial stocks and yielded on average 8% more at Rothamsted in 1973 and 1974 and respectively 5 and 10% more at Woburn in 1973 and 1975. Seed treatments did not consistently affect stem numbers or increase yield although all treatments tended to decrease tuber size. Infection of stem bases and tubers by Polyscytalum pustulans and Rhizoctonia solani was usually less from healthier than from commercial seed and was decreased by benomyl and thiabendazole in 1973 and 1974. Infection by Helminthosporium solani of the skin around tuber eyes was greater from healthier than from commercial seed but was decreased by benomyl and thiabendazole. 2-aminobutane sometimes decreased infection of tubers by P. pustulans and R. solani but neither of stem bases nor of tubers by H. solani. Gangrene on tubers uniformly wounded at lifting was not consistently affected by seed source or seed treatment. Treating seed with benomyl or thiabendazole in 1975 decreased skin spot and black scurf in tubers stored until March 1976. These treatments also decreased silver scurf on the produce of commercial seed at Rothamsted but gangrene was not consistently affected by seed treatments.     

Fungitoxic and phytotoxic effects of fungicides controlling powdery mildew on apple

Benomyl, pyrazophos (HOE 2873), triarimol, triforine and the non-systemic fungicide diethyl phthalimidophosphonothionate (Dowco 199) were sprayed at c. 200 gal/acre (2250 1/ha) at 14-day intervals from pink bud (late April/early May) on seven apple cultivars including Cox's Orange Pippin, Golden Delicious and Jonathan. Compared with binapacryl or dino-cap (and with elemental sulphur on the sulphur-tolerant cv. Golden Delicious), these compounds were more phytotoxic without substantially or consistently improving the control of Podosphaera leucotricha; fruit russet was increased and the crop and fruit size of some cultivars were reduced in some trials. In 1969 the inclusion of bis(dimethylthiocarbamoylthio)-methylarsine (44 ppm) as Urbacid^(R) in the sprays reduced russeting by benomyl and Dowco 199 so that these fungicides became as safe as captan on several cultivars; Dowco 199 was also safened by captan (500 ppm). Urbacid^(R) (88 ppm a.i.) safened benomyl and triarimol on fewer cultivars in 1970 when russet was less severe. The addition of Urbacid^(R) did not prevent yield losses caused by sprays in either year. The crop is most sensitive to spray damage from pink bud until late June. This is a crucial period for the control of mildew, and unless new fungicides are less phytotoxic, palliatives may become necessary adjuvants.     

Effect of Leaf Infection by Kabatiella zeae on Stalk Rot Prevalence and Grain Yield of Maize Hybrids

Eight maize hybrids were tested for resistance to eyespot (Kabatiella zeae) and stalk rot (Fusarium spp.) under field conditions in 1993–1995. The relationship between eyespot and stalk rot and their effect on grain yield of hybrids were studied. Effectiveness of the prochloraz fungicide in controlling both diseases was evaluated. The experiments were performed in three subtrials where plants were naturally infected, inoculated with K. zeae or treated with the fungicide, Sportak 450 EC. The relationship between intensity of leaf damage by eyespot and incidence of stalk rot has been evidently confirmed. High infection of leaf by K. zeae reduced grain yield of hybrids. However, considerable differences in prevalence of disease and yield losses were noted in successive years. The intensity of eyespot depended upon environmental conditions highly variable over the years: it was enhanced by low temperature and high air humidity in July and August. Hybrids showed significant differences in susceptibility to both diseases. Mona – hybrid from the USA and two experimental hybrids from Poland were less affected by diseases than Smolimag and Hidosil (commercial hybrids from Poland). The fungicide Sportak was effective in controlling eyespot and had an indirect influence on decreasing fusarium stalk rot.     

Healthier seed potatoes. I. Effects of inoculating stem cutting stocks with Polyscytalum pustulans and Rhizoctonia solani on growth, yield and disease

Potato seed tubers of seven cultivars derived from stem cuttings in 1965 (healthier seed) were grown in 1969–72 at two sites, one clay with flints soil (Rothamsted) and the other sandy loam soil (Woburn). Inoculating sprouted tubers at planting with Polyscytalum pustulans did not affect the number of stems/plant or total yield but increased stem base and tuber infection. The yields of large tubers (57–83 cm) were increased and small tubers (>57 cm) decreased, indicating a decrease in tuber numbers. Rhizoctonia solani inoculated at planting decreased numbers of stems/plant and yield by up to 14% at Woburn but not at Rothamsted. At both sites, yields of large tubers were increased with cvs Majestic and Record and decreased with Pentland Crown. Stem canker and tuber infection were increased but infection was also prevalent on tubers from non-inoculated seed at Woburn. When both pathogens were inoculated together yields of large tubers were increased in cvs King Edward, Majestic and Record and decreased in Pentland Crown. Infection of stem bases and tubers was sometimes less than when either pathogen was inoculated singly.     

Sources of resistance and susceptibility to Septoria tritici blotch of wheat

An association genetics analysis was conducted to investigate the genetics of resistance to Septoria tritici blotch, caused by the fungus Zymoseptoria tritici (alternatively Mycosphaerella graminicola), in cultivars and breeding lines of wheat (Triticum aestivum) used in the UK between 1860 and 2000. The population was tested with Diversity Array Technology (DArT) and simple‐sequence repeat (SSR or microsatellite) markers. The lines formed a single population with no evidence for subdivision, because there were several common ancestors of large parts of the pedigree. Quantitative trait loci (QTLs) controlling Septoria resistance were postulated on 11 chromosomes, but 38% of variation was not explained by the identified QTLs. Calculation of best linear unbiased predictions (BLUPs) identified lineages of spring and winter wheat carrying different alleles for resistance and susceptibility. Abundant variation in Septoria resistance may be exploited by crossing well‐adapted cultivars in different lineages to achieve transgressive segregation and thus breed for potentially durable quantitative resistance, whereas phenotypic selection for polygenic quantitative resistance should be effective in breeding cultivars with increased resistance. The most potent allele reducing susceptibility to Septoria, on chromosome arm 6AL, was associated with reduced leaf size. Genes which increase susceptibility to Septoria may have been introduced inadvertently into UK wheat breeding programmes from cultivars used to increase yield, rust resistance and eyespot resistance between the 1950s and 1980s. This indicates the need to consider trade‐offs in plant breeding when numerous traits are important and to be cautious about the use of non‐adapted germplasm.     

Effects of fungicides on the growth and conidial germination of Colletotrichum coccodes and on the development of black dot disease of potatoes

The effects of 10 fungicides on the growth of Colletotrichum coccodes in agar culture and on the germination of conidia was investigated. In field experiments in 1990 and 1991 the extent to which treating black dot-affected potato seed tubers with fungicides affected the development of the disease on stem bases, roots and tubers was assessed. Black dot was also assessed on plants from field trials in 1990 which were designed to investigate the efficacy of the soil sterilant 1,3-dichloropropene (Telone) and two nematicides, aldicarb (Temik) and ethoprophos (Mocap). Prochloraz and fenpiclonil were the most effective fungicides in decreasing the size of C. coccodes colonies on agar. Imazalil, propiconazole and dichlorophen were also effective, but at higher concentrations, whereas tolclofos-methyl, thiabendazole and benomyl were only moderately effective. Resistant sectors developed from inhibited colonies on agar containing fenpiclonil and tolclofos-methyl. Conidial germination was prevented at 1 mg/litre fenpiclonil and 5 mg/ litre dichlorophen; imazalil, benomyl and thiabendazole were also moderately effective. Fenpiclonil and propiconazole seed tuber treatments consistently decreased black dot infection on roots, stem bases and daughter tubers early in the season, but only fenpiclonil decreased disease on tubers at harvest in October. Propiconazole also delayed emergence and decreased stem numbers. Soil treatment with 1,3-dichloropropene, aldicarb or ethoprophos had no effect on black dot but Rhizoctonia solani tuber infection and black scurf were increased.     

Fusarium Head Blight of Common Polish Winter Wheat Cultivars – Comparison of Effects of Fusarium avenaceum and Fusarium culmorum on Yield Components

The effects of Fusarium avenaceum and Fusarium culmorum on the reduction in yield components, after independent inoculation of 14 winter wheat cultivars, were investigated. Single isolates of F. avenaceum and F. culmorum were independently used in inoculations of winter wheat heads. Reductions in the following yield traits: 1000‐kernel weight (TKW), the weight (WKH) and number (NKH) of kernels per head after inoculation were analysed statistically. The results indicate differences between both pathogens in their effects on yield traits. The statistical calculations were performed using analysis of variance (a three‐factor experiment) for particular yield trait reductions and multivariate analysis of variance for the yield trait reductions jointly. Almost all of the univariate and multivariate hypotheses concerning no differences between pathogens (F. culmorum, F. avenaceum), climatic conditions (years) and cultivars as well as hypotheses concerning no interactions between factors (pathogens, years, cultivars) were rejected at least at P= 0.05 significance level. The reduction of yield traits indicated individual reactions of the tested winter wheat cultivars to different pathogens. Among the tested traits the highest influence on the rejection of the hypothesis concerning the equivalence of F. avenaceum and F. culmorum was observed for TKW and WKH. The effect of the pathogen on yield reduction was greater for F. avenaceum than for F. culmorum during 1996 and 1997. A comparison of the cultivars indicated that the Begra cultivar showed the highest tolerance to inoculation with both Fusarium pathogens. Moreover, this genotype as well as several others showed lower tolerance to F. avenaceum rather than to F. culmorum, whereas Elena was the only cultivar with the opposite tendency.     

β-1,3-glucanase and chitinase activities in winter triticales during cold hardening and subsequent infection by Microdochium nivale

The accumulation of pathogenesis-related proteins such as β-1,3-glucanases and chitinases was studied in cold induced snow mould resistance in two Polish cultivars of winter triticale, cv. Hewo and cv. Magnat that substantially differ in resistance to Microdochium nivale. The plants were pre-hardened at 12°C for 10 days and hardened at 4°C for 28 days. Subsequently, cold hardened plants were inoculated with fungal mycelium (M. nivale) and incubated at 4°C for 7 days in dark. Cold acclimatisation resulted in suppression of the total glucanase and chitinases activities in the resistant Hewo as well as sensitive Magnat cultivars that possibly coincides with altered metabolism. However, upon infection with M. nivale the chitinases were markedly induced in the cv. Hewo. At the same time, total β-1,3 glucanases activities did not seem to be affected by fungus in any of the tested triticale cultivars. The pattern and/or the activity of chitinases in plants might be indicative for the resistance/susceptibility against M. nivale.     

Epidemiology,identification and disease management of grape black rot and potentially useful metabolites of black rot pathogens for industrial applications – a review

Phyllosticta ampelicida (teleomorph: Guignardia bidwellii) is the fungal plant pathogen that causes black rot on grapevine. It is able to infect all green, expanding grape tissues. Black rot poses a threat to both yield and wine quality; a severe attack can virtually cause a complete crop loss. The fungus is native to North America, and was spread to Europe at the end of the 19th century. With the beginning of the 21st century, an increasing importance of the disease was observed in several European winegrowing regions. Successful black rot control strategies combine sanitary measures, cultural techniques, growing cultivars with reduced susceptibility and the use of effective fungicides. Berries are most susceptible to infections between flowering and bunch closure and consequently, fungicide applications against black rot need to focus on this period. In this paper, forecast models and decision support systems that help to achieve satisfactory control with a minimum of fungicide input are presented. Black rot in grapevine was reported to be well controlled in field experiments by a broad range of fungicide classes including quinone outside inhibitors (98 ± 3% efficacy), demethylation inhibitors (98 ± 3% efficacy) and dithiocarbamates (92 ± 7% efficacy). Average efficacies of other fungicide classes tested so far ranged from 32 to 69%; meta data on the efficacy of fungicides in numerous field trials are given. At present, black rot causes major problems primarily if vineyard management and fungicide use were reduced or abandoned. Organically managed vineyards are highly affected. Hence, site and cultivar selection as well as cultural measures are of the highest importance especially in organic viticulture. Recent studies showed that some strains formerly classified as G. bidwellii in fact belong to a distinct species (P. parthenocissi). Black rot pathogens produce some phytotoxic secondary metabolites, such as phenguignardic acid, guignardic acid, alaguignardic acid and the guignardianones A, E and F, which could stimulate the development of new herbicides of natural origin.