雪雀属系统发育关系的研究（雀形目：文鸟科)

雷雀属Montifringilla7种36个形态特征作为分类单元,进行支序分析。以麻雀属Passer、石雀属Petronia部分种类作为比较外群,讨论雪雀属的分类地位及其与近缘属麻雀属、石雀属系统发育关系;分析了雪雀属内种间的系统发育。支序分析的结果表明雪雀属为单系群,建议将雪雀属划分成两个亚属,讨论了雪雀属种类在进货上的位置,在3个近缘属的系统发育关系中,麻雀属类群较原始,雪雀属类群较进化,石雀属在进货中介于二之间。     

试用聚类方法探讨中国雪雀的分类地位

应用聚类方法对雪雀属、种及部分亚种的分类地位进行初步探索,选取一定的数量性状,取平均值组成原始数据矩阵。原始数据矩阵再进一步标准化,然后应用距离系数和相关系数进行聚类分析。比较了数量性状的聚类结果与传统的形态特征分类之间的异同,并探讨了雪雀属种级分类单元的亲缘关系和分类地位。     

白腰雪雀体重、脂肪累积的阶段性变化

2004年在青海省河南县和兴海县于繁殖早期、育雏期、换羽期等不同阶段用粘网捕获白腰雪雀,称量体重,半定量法测定脂肪的含量,游标卡尺测量泄殖腔的高度,以考察白腰雪雀体重、脂肪的季节性变化.结果 表明:白腰雪雀体重在繁殖早期从2月到3月增加,育雏期下降,换羽期再次增加.脂肪含量在繁殖早期从2月到3月增加,育雏期的6月、7月...     

苔草属复序苔草亚属植物果皮的扫描电镜观察

应用扫描电子显微镜 ,观察了 15种苔草属复序苔草亚属植物果皮的微形态特征。结果表明 ,复序苔草亚属植物果皮的微形态性状多种多样 ,可以为系统学研究提供较丰富的信息 ;并且在所观察的种类中 ,果皮的微形态特征在种内很稳定 ,种间存在不同程度的差异 ,某些近缘种也可表现出一定的相似性。因此 ,上述特征可以做为探讨种之间的分类及亲缘关系的参考。     

群体表型性状研究揭示环境与遗传因素对霍山石斛表型及物种分类的影响

石斛属(Dendrobium)种类繁多, 属内物种具有丰富的表型多样性。霍山石斛(D. huoshanense)为我国特有物种, 其与河南石斛(D. henanense)和细茎石斛(D. moniliforme)以及铁皮石斛(D. catenatum)等近缘种表型相似, 在分类处理中存在争议。这种争议很大程度上与植物普遍存在的表型可塑性和代际共存有关。为探究环境和代际间遗传因素对霍山石斛表型性状的影响以及霍山石斛与近缘种的物种边界问题, 本研究观测了安徽省霍山县霍山石斛(野生、林间和温室F₁代、林间和温室F₂代)、野生河南石斛、细茎石斛和铁皮石斛, 共计16个群体2,279株植株的假鳞茎茎长等12个表型性状; 在种内层面, 首次借鉴生态学同质园实验和遗传学代际间性状比较的方法, 对霍山石斛群体表型性状进行差异显著性检验和95%置信区间比较以及主成分和变异系数等统计学分析。在种间层面, 对霍山石斛与河南石斛和铁皮石斛等近缘种群体表型性状进行比较和分析。结果表明, 环境因素对霍山石斛假鳞茎茎长和假鳞茎直径等具有显著的影响, 代际间遗传因素对霍山石斛假鳞茎直径具有显著的影响。霍山石斛与铁皮石斛和细茎石斛等近缘种群体在假鳞茎茎长、假鳞茎直径、花瓣长和花瓣宽等表型性状方面均存在显著性差异和间隔, 但与河南石斛仅在假鳞茎表型性状方面有显著性差异。我们的研究明确了环境和代际间遗传因素对霍山石斛表型性状的影响程度, 为霍山石斛与近缘种等争议物种的分类和鉴定提供了表型证据。     

同工酶电泳技术在动物分类上的应用

本文系统地介绍了同工酶电泳技术应用于动物分类的理论基础和形式。与传统的分类方法相比较,同工酶电泳技术尚存在一些不足之处,但它弥补了传统方法在种下亚分类方面的缺陷,并且能成功地区分形态上十分接近的近缘种;电泳技术应用于分类学还能精确地阐明种以下各类群间、种间、甚至属间的遗传分化程度。遗传距离是用基因频率的函数表示的群体间的遗传差异;而固定指数则是表示某基因位点等基因频率在不同地区群体间分化程度的测度。     

基于线粒体COI基因片段的麻蝇属(双翅目:麻蝇科)部分种类DNA分类研究

本研究在Pape(1996)提出的麻蝇属(双翅目:麻蝇科)分类系统基础上,选取麻蝇属54个物种(分属于30个亚属),基于线粒体COI基因片段,结合雄性成蝇尾器形态特征,对所选取的30个亚属进行了DNA分类研究,初步探明了各亚属的分类地位与系统发育关系.麻蝇属30个亚属内的平均遗传距离为6.0％(1.8％～11.0％),各亚属间的平均遗传距离为10.1％(5.2％ ～16.1％),亚属内与亚属间遗传距离差异较为明显,说明COI基因片段对麻蝇属各亚属级阶元能进行有效区分.     

中国水鳖科（Hydrocharitaceae）植物的果皮微形态特征

应用扫描电镜对国产水鳖科6属植物的果皮微形态特征进行了初步的观察。结果表明水鳖科各属植物在果皮表面纹饰及表皮细胞形态,径向壁和横向壁凸出与否等特征上存在差异,可以作为属间分类的依据之一。从果皮微形态特征来看,水鳖属和海菜花属差异很大,支持将它们分别作为不同的族处理的观点,而水筛属和苦草属则较为近缘。果皮微形态下的观察结果表明水鳖科和泽泻科植物在果皮表面细胞形态,径向壁明显凸出等方面十分相似,因而支持了水鳖科与泽泻目近缘的观点。     

中国水鳖科( Hydrocharitaceae )植物的果皮微形态特征

应用扫描电镜对国产水鳖科６属植物的果皮微形态特征进行了初步的观察。结果表明水鳖科各属植物在果皮表面纹饰及表皮细胞形态,径向壁和横向壁凸出与否等特征上存在差异,可以作为属间分类的依据之一。从果皮微形态特征来看,水鳖属和海菜花属差异很大,支持将它们分别作为不同的族处理的观点,而水筛属和苦草属则较为近缘。果皮微形态下的观察结果表明水鳖科和泽泻科植物在果皮表面细胞形态,径向壁明显凸出等方面十分相似,因而支持了水鳖科与泽泻目近缘的观点。     

青藏高原白腰雪雀鸣声结构的复杂性

本文以SAS Lab鸟类声谱分析软件对采自青藏高原黑马河、玛多与花石峡、沱沱河的白腰雪雀不同地方种群的鸣叫与鸣唱的复杂性进行了分析 ,发现白腰雪雀的鸣叫相对变化率随着采样量的增加由 0 5 2 2迅速降低到 0 1 2 7和 0 1 1 9,表明其鸣叫声组成中音节类型较少。同时发现其声谱图结构比较简单 ,表现出高度的音节重复并多有谐波的现象。鸣唱结构相对复杂 ,不同鸣唱音节数随鸣唱曲目的增加 ,开始有明显的增加 ,当鸣唱曲目增加到一定值时 ,不同音节数的增加趋于平缓 ,几乎保持稳定 ;野外未发现任何相同的鸣唱型 ,但在不同鸣唱型之间具有不同程度的音节共享现象。依据雷富民等 (2 0 0 3)对鸟类鸣唱多样性和复杂性的评述 ,白腰雪雀鸣唱的结构模式符合“多音节序列不稳定变化型” ,其鸣唱曲目中音节的转换形式为“序列鸣唱”。然而 ,鸣唱模式中音节类型的有限性和鸣唱型的高度多样化表明 :白腰雪雀鸣唱的复杂性不仅体现在鸣唱型内较丰富的音节组成 ,而且更重要的还在于不同鸣唱型间具有多变的音节序列组合形式     

Molecular phylogenetic relationship of snow finch complex (genera Montifringilla, Pyrgilauda, and Onychostruthus) from the Tibetan plateau

The snow finch complex (Montifringilla, Pyrgilauda, and Onychostruthus) has its center of distribution on the Tibetan plateau, with six out of seven species in the genera occurring there. Phylogenetic relationships among these six species of three genera have been studied based on DNA sequence data obtained from the mitochondrial cytochrome b gene and the nuclear myoglobin gene. The results support monophyly of the snow finch complex group and three major evolutionary lineages are recognized. The first clade consists of ruficollis, blanfordi, and davidiana. These three taxa are sometimes placed in their own genus, Pyrgilauda, and the DNA data supports this. The three taxa nivalis, henrici, and adamsi have traditionally been placed in the genus Montifringilla, and they group together strongly in the present analysis. The results further suggest that nivalis and adamsi are more closely related to each other than are nivalis and henrici, despite that the latter two are often regarded as conspecific. The third distinct lineage within the snow finch complex consists of taczanowskii, which has been placed its own genus, Onychostruthus. This taxon has a basal position in the phylogenetic tree and is sister to all other snow finches. We estimated that taczanowskii split from the other taxa between 2 and 2.5 mya, i.e., about the time for the most recent uplift of the Tibetan plateau, "the Tibet movement", 3.6-1.7 mya. Cladogenesis within the Montifringilla and Pyrgilauda clades seems to be contemporary with the second phase of "Tibet movement" at 2.5 mya and the third phase at 1.7 mya and "Kunhuang movement" in 1.5-0.6 mya. The dramatic climatic and ecological changes following from the uplift of the Tibetan plateau, together with the cyclic contraction and expansion of suitable habitats during the Pleistocene, are probably the most important factors for the cladogenesis in snow finch complex.     

A morphological and chemical analysis of geographical variation in Tilia L. of eastern North America

A statistical analysis of morphological variation and a Chromatographic analysis of flavonoid variation were performed to determine taxonomic relationships among the species ofTilia of eastern North America. No apparent morphological discontinuities were seen between populations within the sample area although two characters (involving leaf pubescence and gland length) showed definite patterns of geographical variation. Flavonoid patterns showed definite differences between northern and southern populations with an intermediate zone in the Smoky Mountain region. The continuous nature of the morphological and flavonoid variation suggested that the genus as represented in eastern North America should be regarded as one species,Tilia americana L.     

A study of genetic variation and evolution of Phyllostachys (Bambusoideae: Poaceae) using nuclear restriction fragment length polymorphisms

Phylogenetic and taxonomic difficulties are common within the woody bamboos, due to their unique life cycle, which severely limits the availability of floral characters. To addresss some of these problems, 20 species of woody bamboos in the genus Phyllostachys were analyzed using nuclear restriction fragment length polymorphisms (RFLPs). The RFLP data were used to generate genetic distances between all pairs of taxa and to examine the degree of genetic variation within and among bamboo species. The genetic distances were also used to create dendrograms of accessions and species. These trees supported the current division of the genus into two sections and provided some information on the thorny taxonomic problems in this group. We show that RFLPs can be used for species identification and the delineation of species limits.     

Multivariate analysis of morphological variation among closely related species Bromus japonicus, B. squarrosus and B. arvensis (Poaceae) in comparison with isozyme evidences

Morphological variation and taxonomic continuum of 110 specimens of Bromus arvensis, B. japonicus and B. squarrosus were analysed to assess the morphological variability of these close taxa. The importance of every morphological character was estimated by the GLM procedure. Stepwise discriminant analysis was used in order to find a set of morphological characters that maximises differences among the species. According to this, the most important characters appeared to be the length of anthers, width of lemma and lemma margin angle shape. The most important characters for the determination of Bromus arvensis was the length of anthers and for B. squarrosus width of lemma. According to the UPGMA cluster analysis, canonical discriminant, classificatory discriminant and principal component analyses, all specimens were separated into three moderately distinct groups which corresponded to the three traditional species. The study showed that qualitative characters were the best for the delimitation of the tax a by statistical analyses and demonstrated the incongruity between the pattern of morphological and genetic (allozyme) variation among the three Bromus species.     

高海拔和高纬度生境的相似性:综述以及青藏高原鸟类肾上腺皮质激素对应激反应的初步研究

在介绍性教科书、保护计划甚至基础著作中 ,常常将北极区和高山生物群系等同看待。高山和北极区生物群系无树的广阔区域 ,草本和非禾本科草本植物的初级生产力总量低 ,食物网单一。此外 ,除热带高山生境可能有例外 ,该两种环境都具有明显的季节性。但是 ,对于这些表面上相似的环境以前很少进行比较 ,而两者之间应具有明显的不同。我们假定 :如果环境相似 ,栖息在这些环境里的鸟类会对意外的应激刺激表现出相似的反应。相反 ,如果这些环境很不同 ,那么鸟类对剧烈应激的反应可进行调整以适应当地的不同环境 ,而不应一成不变。本文初步报道了青藏高原某些繁殖鸟类的肾上腺皮质反应 ,并与已发表的栖息于北极区苔原生态系统鸟类的数据进行了比较。白腰雪雀 (Onychostruthustaczanowskii)和棕颈雪雀 (Pyrgilaudaruficollis)被认为是高山代表物种 ,随着标准化捕捉 -处理 -抑制的应激刺激 ,它们表现出典型的血浆肾上腺皮质酮含量升高。但是 ,不同于许多北极区的代表物种 ,它们对应激的肾上腺皮质反应并无任何季节性变化。研究结果表示 :生活在高山和北极区环境下的鸟类物种可能具有激素反应以适应其生存环境所施加的生态挑战 [动物学报 49(1) :1～ 19,2 0 0 3]。     

Genetic variation in two sympatric European populations of Bosmina spp. (Cladocera) tested with RAPD markers

We used RAPDs (Random Amplified Polymorphic DNA) to test genetic divergence between two populations of Bosmina spp. in Lake Östersjön, Sweden. Previous taxonomic studies on European species within the genus Bosmina have been based on morphological characters alone. RAPD markers distinguished the two populations and supported the specific status of B. coregoni and B. longispina based on morphological characters. Furthermore, juveniles with a long antennule and a mucro were classified as B. coregoni. RAPDs also revealed genetic differences among the tested individuals, suggesting several clones within each species.     

Biosystematic Studies on Adenophora potaninii Korsh. Complex (Campanulaceae). Ⅰ. Phenotypic Plasticity

Phenotypic plasticity is the environmental modification of genotypic expression and an important means by which individual plants respond to environmental heterogeneity. The study of phenotypic plasticity in the genus Adenophora, which is very complicated taxo nomically because of great morphological variation, proves to be helpful in both investigating the phenotypic variation so as to evaluate potential taxonomic value of their characters and providing important sources of information on the variation, adaptation and evolution of the genus. Twenty-three populations representing all the six species in Adenophora potaninii complex were transplanted into the garden. Of them six populations were selected for study ing their performance in the field and in the garden, in addition to cultivation experiment under different treatments. The results show that there exists considerable developmental plasticity in some leaf, floral and capsule characters. In particular, the leaf shape and length of calyx lobe display significant developmental variation with the maximum being three times as great as the minimum, which is noteworthy because they were previously considered as diagnostic. The characters of root, caudex, stem and inflorescence are found to be very plastic, especially the root diameter, the number of stems, stem height and inflorescence length with great environmental plasticity. In addition, the populations from different habi tats show distinct amounts of plasticity. On the contrary, the characters of leaf, floral, cap sule and seed are less influenced by environments. It seems that the considerable variation in the characters of leaf is attributed mainly to genetic differences. Finally, the phenotypic plasticity of morphological characters of A. potaninii complex and its taxonomic significanceis discussed.