粘虫核糖体蛋白S7cDNA的克隆和表达分析

运用RT—PCR和RACE技术克隆了粘虫Mythimnaseparata（Walker）核糖体蛋白s7基因（RPS7）的全长eDNA序列（GenBank登录号：JN582331）,并对其进行生物信息学分析。结果表明,粘虫RPS7全长eDNA序列为762bp,包括5’非编码区32bp和3’非编码区67bp。其开放阅读框（573bp）编码190氨基酸肽链,具有核糖蛋白S7e蛋白家族典型特征。该肽链理论分子量为21．924ku,等电点为9．82,富含4种类型的特定功能位点。该蛋白序列与其他动物RPS蛋白序列具有96．8％-98．2％高度同源性。应用荧光实时定量技术建立了粘虫脑部胚后发育RPS7表达模式。RPS7表达量随胚后发育脑部重建呈现出动态变化,这一结果显示RPS7是在转录水平上呈现发育性调节。     

粘虫核糖体蛋白S7 cDNA的克隆和表达分析

运用RT-PCR和RACE技术克隆了粘虫Mythimna separata(Walker)核糖体蛋白S7基因(RPS7)的全长cDNA序列(GenBank登录号:JN582331),并对其进行生物信息学分析。结果表明,粘虫RPS7全长cDNA序列为762 bp,包括5'非编码区32 bp和3'非编码区67 bp。其开放阅读框(573 bp)编码190氨基酸肽链,具有核糖蛋白S7e蛋白家族典型特征。该肽链理论分子量为21.924 ku,等电点为9.82,富含4种类型的特定功能位点。该蛋白序列与其他动物RPS蛋白序列具有96.8%～98.2%高度同源性。应用荧光实时定量技术建立了粘虫脑部胚后发育RPS7表达模式。RPS7表达量随胚后发育脑部重建呈现出动态变化,这一结果显示RPS7是在转录水平上呈现发育性调节。     

油菜叶绿体核糖体蛋白基因rps7的克隆和序列分析

从甘蓝型油菜（Ｂｒａｓｓｉｃａ ｎａｐｕｓ ｃｖ．Ｈ１６５）叶绿体基因组克隆得到了编码核糖体蛋白的基因ｒｐｓ７。经序列分析得知，该基因编码区包含个核苷酸，编码一个分子量为２０ １０９ Ｄ、由１５５个氨基酸组成的蛋质。该基因的核苷酸和编码的氨基酸序列与烟草对应基因的同源性皆高达９７％；而与水稻对应基因的同源性为９０％和８４％。该基因不含内含子，没有典型的ＳＤ序列，但在５’端－２５～－２２位发现一个与     

舌鳎亚科鱼类单系起源和同种异名的线粒体DNA证据

舌鳎亚科鱼类因形态特征的特殊性, 其分类及系统发育关系一直存在争议。本研究测定了中国沿海14种舌鳎亚科鱼类线粒体DNA的16S rRNA和Cyt b基因的部分片段。两个基因构建的舌鳎亚科系统发育树结果显示, 中国沿海舌鳎亚科鱼类为明显的单系群, 但舌鳎亚科鱼类内部的系统发育关系与形态分类划分的亚属并不完全一致, 日本须鳎(Paraplagusis japonica)与其他舌鳎属(Cynoglossus)种类并未形成不同的分支。虽然舌鳎属内的舌鳎亚属(Cynoglossus)、拟舌鳎亚属(Cynoglossoides)和三线舌鳎亚属(Areliscus)均可以聚为独立分支, 但长吻红舌鳎(C. lighti)与短吻红舌鳎(C. joyneri)、短吻三线舌鳎(C. abbreviatus)与紫斑舌鳎(C. purpureomaculatus)、半滑舌鳎(C. semilaevis)与窄体舌鳎(C. gracilis)及褐斑三线舌鳎(C. trigrammus)这三组物种可能存在同种异名现象。这一结果提示, 基于形态学对舌鳎亚科的种属分类鉴定尚存在不足, 线粒体DNA的系统发育关系可为其分类的修订提供有意义的参考和佐证。     

油菜叶绿体核糖体蛋白基因rps7的克隆和序列分析(英文)

从甘蓝型油菜 (Brassicanapuscv .H1 65)叶绿体基因组克隆得到了编码核糖体蛋白的基因rps7。经序列分析得知 ,该基因编码区包含 468个核苷酸 ,编码一个分子量为 2 0 1 0 9D、由 1 55个氨基酸组成的蛋白质。该基因的核苷酸和编码的氨基酸序列与烟草对应基因的同源性皆高达 97% ;而与水稻对应基因的同源性分别为 90 %和 84%。该基因不含内含子 ,没有典型的SD序列 ,但在 5’端 - 2 5～- 2 2位发现一个与烟草psbA基因的顺式作用元件RBS2完全相同的TGAT框。与烟草和水稻的同源序列比较 ,该基因在 3’端非编码区变异较大 ,发生了多次插入和缺失。构建了包含该基因在内的一个 1 .0kbDNA的限制性内切酶图谱。所报告的基因序列已登录GenBank。     

云南鲤科鱼类一新属新种(鲤形目:鲤科:(鱼丹)亚科)

本文记述采自云南澜沧江水系的鲤科(鱼丹)亚科鱼类一新属新种。新属裸(鱼丹)属Gymnodanio gen.nov.在侧线、臀鳍条数目等特征上近似于低线(?)属Barilius,但以其除侧线鳞外体裸露无鳞,具不完全之腹棱等而与其及(鱼丹)亚科现有各属相区别。新种命名为条纹裸(鱼丹)G.strigatus sp.nov.。     

水稻胞质核糖体蛋白基因OsRPS7的克隆与序列分析

以已公布的黑麦胞质核糖体蛋白基因ScRPS7的cDNA序列为信息探针,在中国华大水稻基因组数据库中搜索与之高度同源的基因组重叠群。采用计算机拼接和RT-PCR方法克隆了水稻胞质核糖体蛋白基因的全长cDNA序列,命名为OsRPS7。该cDNA序列全长919bp,编码192个氨基酸;其与黑麦、拟南芥和芸薹的S7核糖体蛋白的氨基酸一致率分别为88%、72%和72%。对OsRPS7 的基因组结构和基因的功能进行了分析和预测。Abstract：Using the cDNA of rye cytoplasmic ribosomal protein ScRPS7 as a query probe, a highly homologous rice genomic contig was obtained from Huada rice genome database. The full-length cDNA sequence of rice cytoplasmic ribosomal protein S7 was assembled by informatics based on the contig. Furthermore, with the two primers designed according to this assembled cDNA, the full-length cDNA of rice ribosomal protein was cloned by RT-PCR and named as OsRPS7. The cDNA was 919bp in length and contained a complete Open Reading Frame (ORF) of 576bp, encoding a protein of 192 amino acid residues. The deduced amino acids of OsRPS7 showed 88%、72% and 72% identity with those from Secale cereale、Arabidopsis thaliana and Brassica oleracea, respectively. The genome structure of OsRPS7 was analyzed, and its function was predicted in this paper.     

泛肽、核糖体蛋白及泛肽-核糖体蛋白S27a与肿瘤的关系

泛肽-核糖体蛋白S27a(Ubiquitin-ribosomal protein S27a,UBRS27a)是泛肽和核糖体蛋白的融合蛋白,N端为泛肽,C端由含C2-C2型锌指结构域的高度保守核糖体蛋白S27a构成。在真核细胞中表达时,被酶解成泛肽和核糖体蛋白。该多功能核糖体蛋白在各种活性增殖细胞和瘤组织中高度表达,在多种类型的肿瘤细胞中,该基因的过量表达是一个典型特征。本实验室对该蛋白在家蚕中的作了初步研究,也发现RPS27a在活性增殖细胞中表达量很高。大多数核糖体蛋白的功能还没有完全探明,它们不仅仅在组装成核糖体时起作用,往往还有核糖体外的功能。回顾了最近几年有关该融合蛋白以及与它相关的泛肽途径、核糖体蛋白与肿瘤之间的关系。通过对它们的研究,有可能预示肿瘤的发生和发展,并为肿瘤临床诊断提供依据,为恶性肿瘤的治疗提供靶点。     

泛肽、核糖体蛋白及泛肽-核糖体蛋白S27a与肿瘤的关系

泛肽-核糖体蛋白S27a（Ubiquitin-ribosomal protein S27a,UBRS27a）是泛肽和核糖体蛋白的融合蛋白,N端为泛肽,C端由含C2-C2型锌指结构域的高度保守核糖体蛋白S27a构成。在真核细胞中表达时,被酶解成泛肽和核糖体蛋白。该多功能核糖体蛋白在各种活性增殖细胞和瘤组织中高度表达,在多种类型的肿瘤细胞中,该基因的过量表达是一个典型特征。本实验室对该蛋白在家蚕中的作了初步研究,也发现RPS27a在活性增殖细胞中表达量很高。大多数核糖体蛋白的功能还没有完全探明,它们不仅仅在组装成核糖体时起作用,往往还有核糖体外的功能。回顾了最近几年有关该融合蛋白以及与它相关的泛肽途径、核糖体蛋白与肿瘤之间的关系。通过对它们的研究,有可能预示肿瘤的发生和发展,并为肿瘤临床诊断提供依据,为恶性肿瘤的治疗提供靶点。     

中国( )属鱼类的研究(鲤形目:鲤科)

中国Ｄａｎ属鱼类共有７种,分布于云南南部和西南部的三大流域中。其中半线Ｄａｎ Ｄａｉｏ ｉｎｔｅｒｒｕｂｔｕｓ,红蚌Ｄｎａ Ｄ．ｋａｋｈｉｅｎｅｎｓｉｓ,缺须Ｄａｎ Ｄ．ａｐｏｇｏｎ和珍珠Ｄａｎ Ｄ．ａｌｂｏｌｉｎｅａｔｕｓ分布地伊洛瓦底江流域的大盈江和龙川江水系,布良Ｄａｎ Ｄ．ｂｒｏｗｎｉ和撞邦Ｄａｎ．Ｄ．ｓｈａｎｅｎｓｉｓ分布于萨尔温江上游的怒江水系,金线Ｄａｎ Ｄ．ｃｈｒｙｓｏｔａｅｎｉａ     

似鮈类鱼类的系统发育研究（鲤形目：鲤科）

本文通过对似类８属１３种和外类群７属１０种鱼类的比较研究,选取３７个骨骼及外部特征,运用外类群比较法进行特征分析,根据分支系统学原理重建了似类的系统发育。特征分析和系统发育分析表明似类是具有许多共同离征的单系群,朝着流水性底栖生活和刮食性的主干方向发展;其中以似属最原始,胡属最特化构成７对姐妹群。蛇属是较早分化出的相对特化的类群,棒花鱼属和小鳔属的关系相对较远。     

Molecular phylogenetics of the family Cyprinidae (Actinopterygii: Cypriniformes) as evidenced by sequence variation in the first intron of S7 ribosomal protein-coding gene: further evidence from a nuclear gene of the systematic chaos in the family

The family Cyprinidae is the largest freshwater fish group in the world, including over 200 genera and 2100 species. The phylogenetic relationships of major clades within this family are simply poorly understood, largely because of the overwhelming diversity of the group; however, several investigators have advanced different hypotheses of relationships that pre- and post-date the use of shared-derived characters as advocated through phylogenetic systematics. As expected, most previous investigations used morphological characters. Recently, mitochondrial DNA (mtDNA) sequences and combined morphological and mtDNA investigations have been used to explore and advance our understanding of species relationships and test monophyletic groupings. Limitations of these studies include limited taxon sampling and a strict reliance upon maternally inherited mtDNA variation. The present study is the first endeavor to recover the phylogenetic relationships of the 12 previously recognized monophyletic subfamilies within the Cyprinidae using newly sequenced nuclear DNA (nDNA) for over 50 species representing members of the different previously hypothesized subfamily and family groupings within the Cyprinidae and from other cypriniform families as outgroup taxa. Hypothesized phylogenetic relationships are constructed using maximum parsimony and Basyesian analyses of 1042 sites, of which 971 sites were variable and 790 were phylogenetically informative. Using other appropriate cypriniform taxa of the families Catostomidae (Myxocyprinus asiaticus), Gyrinocheilidae (Gyrinocheilus aymonieri), and Balitoridae (Nemacheilus sp. and Beaufortia kweichowensis) as outgroups, the Cyprinidae is resolved as a monophyletic group. Within the family the genera Raiamas, Barilius, Danio, and Rasbora, representing many of the tropical cyprinids, represent basal members of the family. All other species can be classified into variably supported and resolved monophyletic lineages, depending upon analysis, that are consistent with or correspond to Barbini and Leuciscini. The Barbini includes taxa traditionally aligned with the subfamily Cyprininae sensu previous morphological revisionary studies by Howes (Barbinae, Labeoninae, Cyprininae and Schizothoracinae). The Leuciscini includes six other subfamilies that are mainly divided into three separate lineages. The relationships among genera and subfamilies are discussed as well as the possible origins of major lineages.     

鳅鮀鱼类鳔囊结构及系统发育研究(鲤形目:鲤科)

本文应用分支系统学原理对鳅(鱼它)鱼类的鳔囊、鳔及相关结构进行了特征分圻,并以(鱼句)亚科作为外类群。解剖结果表明,鳅(鱼它)鱼类的鳔分四种类型;分析结果进一步证实鳅(鱼它)鱼类是一个单源群,作为一亚科可分为异鳔鳅(鱼它)属和鳅(鱼它)属,后者又分为原鳅(鱼它)和鳅(鱼它)二个亚属。     

基于12S rRNA基因的鹳形目系统发生关系

采用分子系统学的方法探讨鹳形目5个科之间的系统发生关系.文中测出鹳形目鸟类7种mtDNA 12SrRNA基因全序列,并结合来自Genbank的鹳形目另外7个物种及原鸡的同源区序列,经Clustal W软件对位排列后共1 009位点,包含405个变异位点,其中多态性位点381个,260个简约信息位点.基于上述序列数据,以原鸡为外群,使用距离邻接法、最大简约法、最大似然法及贝叶斯法分别重建了鹳形目5科14种的系统发生树.重建的系统发生树显示,内群中的14个种聚合为4支:鹮科构成第一支,聚在系统树的基部;锤头鹳科与鲸头鹳科聚为一支;鹭科和鹳科各自聚成一支.在比较不同建树方法的结果并进行合意树分析后认为:在鹳形目的系统发生中,鹮科可能是最早分化出的一支;锤头鹳科与鲸头鹳科之间的亲缘关系最近,它们祖先与鹭科、鹳科之间的分歧在时间上可能非常接近.鹳形目5个科之间的系统关系可以表示为:(鹮科,(鹭科,鹳科,(锤头鹳科,鲸头鹳科))).     

基于12S rRNA基因序列探讨崇安地蜥的分类地位

为探讨崇安地蜥Platyplacous sylvaticus的分类地位,测定了崇安地蜥线粒体12S rRNA基因全序列,并从GenBank中下载了东亚产10种草蜥、3种地蜥的同源序列进行分析,采用Mega V2.1软件的NJ法和ME法、PAUP4.0软件的MP法构建分子系统树.结果表明:崇安地蜥线粒体12S rRNA基因全序列(952 bp)中T、C、A、G碱基含量分别为23.1%、22.9%、35.9%、18.1%;与其它同源序列比对后有978 bp,发现321个位点出现变异,占总位点数32.8%,其中199个简约信息位点,为总变异位点的62%;转换/颠换之比平均为2.16.构建的分子系统树中,NJ树和ME树完全一致,与MP树略有差异.3种构树法中崇安地蜥与南台草蜥Takydromus sauteri、峨眉地蜥P.intermedius、先岛地蜥P.dorsalis均聚为一支,崇安地蜥与先岛地蜥亲缘关系最近.本实验结果支持将地蜥属并入草蜥属和取消地蜥亚属的观点.     

THE ROLE OF INTROGRESSIVE HYBRIDIZATION IN THE EVOLUTION OF THE GILA ROBUSTA COMPLEX (TELEOSTEI: CYPRINIDAE)

Abstract The extent and impact of introgressive hybridization was examined in the Gila robusta complex of cyprinid fishes using mitochondrial DNA (mtDNA) sequence variation. Lower Colorado River basin populations of G. robusta, G. elegans, and G. cypha exhibited distinct mtDNAs, with only limited introgression of G. elegans into G. cypha. The impact of hybridization was significant in upper Colorado River basin populations; most upper basin fishes sampled exhibited only G. cypha mtDNA haplotypes, with some individuals exhibiting mtDNA from G. elegans. The complete absence of G. robusta mtDNA, even in populations of morphologically pure G. robusta, indicates extensive introgression that predates human influence. Analysis of the geographic distribution of variation identified two distinctive G. elegans lineages; however, the small number of individuals and localities sampled precluded a comprehensive analysis. Analysis of haplotype and population networks for G. cypha mtDNAs from 15 localities revealed low divergence among haplotypes; however, significant frequency differences among populations within and among drainages were found, largely attributable to samples in the Little Colorado River region. This structure was not associated with G. cypha and G. robusta, as morphotypes from the same location are more similar than conspecific forms in other locations. This indicates that morphological and mtDNA variation are affected by different evolutionary forces in Colorado River Gila and illustrates how both hybridization and local adaptation can play important roles in evolution.     

PHYLOGENY OF THE BATRACHOSPERMALES (RHODOPHYTA) INFERRED FROM rbcL AND 18S RIBOSOMAL DNA GENE SEQUENCES

The sequence data from the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase ( rbc L) gene and 18S ribosomal DNA (small subunit) of taxa in the freshwater rhodophyte order Batrachospermales were used to construct phylogenetic hypotheses. Taxa examined in this study represent four families, eight genera, and six sections of the genus Batrachospermum . In addition, Rhododraparnaldia oregonica Sheath, Whittick et Cole, was included in the analysis because it shares particular ultrastructural, reproductive, and morphological characteristics with members of the Batrachospermales and Acrochaetiales. The trees generated from each gene, as well as a combined data set, were largely congruent. Rhododraparnaldia consistently occurs on an early branch within the Acrochaetiales – Palmariales clade and does not appear to be a member of the Batrachospermales. In addition, Thorea violacea Bory de St. Vincent was not closely related to the other taxa of the Batrachospermales in all trees and hence the family Thoreaceae does not appear to be a natural grouping within this order. All other taxa analyzed, which are presently classified within this order, formed a monophyletic clade in most analyses. Psilosiphon scoparium Entwisle was not closely allied with the taxa of the Lemaneaceae, lending support to the newly proposed family Psilosiphonaceae. Sequence data from the remaining taxa of the Lemaneaceae support the concept of a derived monophyletic clade. The genus Batrachospermum appears to comprise many morphologically similar but distantly related taxa, which will need further investigation to resolve their taxonomic status. Tuomeya, Sirodotia and Nothocladus are retained at the generic level until further data are obtained.     

棉铃虫18S核糖体RNA基因的序列分析及其分子系统学

克隆并分析了鳞翅目棉铃虫Helicoverpa armigera (Hübner)18S核糖体RNA基因(18S rDNA)的全序列,将该序列与鞘翅目、膜翅目、同翅目、双翅目、捻翅目和弹尾目各一种昆虫的同源保守区进行了比较。序列分析结果显示：鳞翅目和双翅目昆虫在18S rDNA结构上彼此较为相似,捻翅目昆虫的18S rDNA分子结构表现出与其它目昆虫有较大的差异,但相对与弹尾目昆虫的18S rDNA较为接近。该结果支持了有关捻翅目属于一个独立的目级分类阶元的论点。     

淡水鱼类粘孢子虫的18S rDNA分子系统学研究

利用两个通用引物myxoF(5‘-CGCGGTAATTCCAGCTCCAGTAG-3‘)和myxoR(5-ACCAGGTAAGTTTTCCGTGTTGA-3’)成功扩增出圆形碘泡虫、全圆碘泡虫、武汉单极虫、微山尾孢虫和库班碘泡虫5种粘孢子虫的部分18S rDNA序列,其GenBank登录号为：AY165179-AY165183。并结合GenBank其他13个相关序列构建了18个物种的分子系统树。结果表明,碘泡虫,尾孢虫和单极虫较Tetracapsula bryozoides和“PKX”分化晚,它们形成了2个聚类：T．wuhanensis-T.hovorkai-M.rotundatus-M.rotundus-M.bidullatus-M.pellicides-M.pendula-H.salminicola聚类和H.exilis-H.ictaluri-M.spinacurvatura-M.osburni-H.lesteri-H.weishanensis-M.kubanicum的聚类;同一属内,采自国内的种类并没有因为地域关系而形成独立的分支,而是与国外的种类交叉在一起,这意味着粘孢子虫种类之间的地域差别并不大;碘泡虫和尾孢虫在进化上都不是单系的,而且分子数据难以将这两个属的种类分开,因此尾孢虫的尾突可能并不是有效的分类依据,而是和碘泡虫的壳片突起同源的一种附属结构。     

属鱼类的分类整理（鲤形目：鲤科）

本文对鲤科、亚科的属鱼类分类进行全面的整理和研究,除增补和作必要更动外,还描记二新种,间,新种Ｈｅｍｉｂａｒｂｕｓｍｅｄｉｕｓｓｐ．ｎｏｖ．和短鳍,新种Ｈｅｍｉｂａｒｂｕｓｂｒｅｖｉｐｅｎｎｕｓｓｐ．ｎｏｖ,重整后本属共计８种。