猴源细小病毒血凝和血凝抑制试验的优化与应用

目的优化猴源细小病毒的血凝和血凝抑制（HA/HI）试验条件,以优化的方法对160份2010年～2011年间采集于中国北京地区圈养猴群的血清样品进行猴源细小病毒抗体调查。方法 在不同的缓冲液、缓冲液pH值、猪红细胞浓度、兔血清浓度、阿氏液比例和温度下进行猴源细小病毒的HA/HI试验,选择合适的HA/HI条件。通过优化的HA/HI方法对160份猴血清进行猴源细小病毒抗体检测。结果pH 7.0、0.02 mol/L PBS、0.75%猪红细胞、0.5%兔血清和4℃可作为猴源细小病毒HA/HI试验合适的反应条件,猪血球以1∶1阿氏液抗凝,可保存5～7 d不影响实验结果。猴血清的猴源细小病毒抗体阳性率为58.1%。结论方法优化后稳定性增强、检测时间缩短、准确性提高。细小病毒在我国北京地区圈养猴群中感染比较普遍。     

Cimulative hemagglutination by Mycoplasma pneumoniae and other agglutinins

John, T. Jacob (University of Colorado School of Medicine, Denver), Marlene Stahl, and Vincent A. Fulginiti. Cumulative hemagglutination by Mycoplasma pneumoniae and other agglutinins. J. Bacteriol. 92:1002-1004. 1966.-The phenomenon of cumulative hemagglutination, or hemagglutination by two agglutinins, each in subagglutinating concentration, was demonstrated by use of four different systems, namely, horse serum and Mycoplasma pneumoniae, horse serum and measles antigen, M. pneumoniae and measles antigen, and parainfluenza 2 virus and M. pneumoniae. Cumulative hemagglutination appears to be the mechanism by which a horse-serum diluent causes high hemagglutination titers of M. pneumoniae, since both contain hemagglutinins against vervet erythrocytes. It was also shown that antibodies against either one of the two antigens may cause inhibition of such hemagglutination.