A simple,rapid assay for cysteamine and other thiols

Cysteamine is under investigation as an aid in radiation therapy and as a treatment for the inherited disorder cystinosis. An assay is presented for its measurement in biological fluids. The specific reaction of thiosulfonates with sulfhydryl compounds is employed to form a radiolabeled derivative of cysteamine which is then isolated by high-voltage electrophoresis on paper. Cysteamine can be measured in aqueous solutions, plasma, and urine with this method.     

Factors influencing performance in the Hecht vault and implications for modelling

This paper investigated the factors that influence Hecht vault performance and assessed the level of model complexity required to give an adequate representation of vaulting. A five-segment planar simulation model with a visco-elastic shoulder joint and a torque generator at the shoulder joint was used to simulate the contact phase in vaulting. The model was customized to an elite gymnast by determining subject-specific segmental inertia and joint torque parameters. The simulation model was matched to a performance of the Hecht vault by varying the visco-elastic characteristics of the shoulders and the arm-horse interface and the activation time history of the shoulder torque generator until the best match was found. Perturbing the matching simulation demonstrated that appropriate initial kinematics are necessary for a successful performance. Fixing the hip and knee angles at their initial values had a small effect with 3 degrees less rotation. Applying shoulder torque during the contact phase also had a small effect with only a 7 degrees range in landing angles. Excluding the hand segment from the model was found to have a moderate effect with 15 degrees less rotation and the time of contact reduced by 38%. Removing shoulder elasticity resulted in 50 degrees less rotation. The use of a five-segment simulation model confirmed that the use of shoulder torque plays a minor role in vaulting performance and that having appropriate initial kinematics at touchdown is essential. However, factors such as shoulder elasticity and the hands which have previously been ignored also have a substantial influence on performance.     

Factors influencing the distribution of Azorean mountain vegetation: implications for nature conservation

In order to understand the main ecological factors that influence the distribution of mountain vegetation in Azores and to use it as a model for nature conservation, a study on Santa Bárbara Mountain on Terceira Island was performed. A multivariate analysis was done on vegetation data and the abiotic factors governing the vegetation pattern are discussed. The major factors contributing to the observed vegetation patterns are wind exposure, soil water saturation and historical human impacts. The vegetation analysis showed that Azorean Mountain habitats have a high variation of vegetation structure as a result of the habitat heterogeneity and the extreme ecological conditions. Using the co-dominant plants as indicator species, eco-zones are distinguished, characterized by the combination of different ecological–human factors which contribute to the distribution of specific types of natural vegetation communities. The studies of vegetation distribution give important information about the relation between vegetation and landscape that is essential for conservation, restoration proposals and landscape planning. Azores terrestrial Natura 2000 sites are largely dominated by mountain vegetation, therefore this work provides essential background data for the management plans of these sites.     

Factors influencing the oxidation of the radioprotector WR-1065

N-(2-Mercaptoethyl)-1,3-diaminopropane (WR-1065) is the free thiol form of the radio- and chemoprotector S-2-(3-aminopropylamino)ethylphosphorothioic acid (WR-2721). Interest currently exists in the clinical use of WR-2721 and WR-1065 as radio- and chemoprotectors of normal tissues. However, measurement of plasma levels of WR-1065 has proven difficult, due to rapid drug oxidation. Therefore, we studied factors influencing the oxidation of WR-1065, in Hepes-buffered saline as well as in tissue culture media containing 10% fetal bovine serum. The rate of oxygen consumption by WR-1065, as determined using the Clark oxygen electrode system, was faster in medium plus serum than in Hepes-buffered saline. That this effect is largely due to the presence of trace metal ions in tissue culture media and serum was indicated by the observation that addition of Cu2+ or Fe3+ to buffer stimulated oxygen consumption. Addition of KCN inhibited the reaction of WR-1065 with oxygen, and this effect was dependent on KCN concentration. That KCN blocked WR-1065 oxidation to the disulfide was verified using Ellman's reagent to quantitate the free thiol form. The rate of oxygen consumption was shown to be affected by temperature as well as concentration of WR-1065. Catalase reduced the rate of oxygen consumption of WR-1065, indicating that peroxide is formed in this system. Superoxide dismutase had a stimulatory effect. WR-1065 was found to stimulate the hexose monophosphate shunt in A549 cells. Since this stimulation was prevented by the presence of catalase, it appeared to be due to the response of the cells to peroxide, formed as a result of WR-1065 autooxidation.     

Radiation carcinogenesis in experimental animals and its implications for radiation protection

Cancer induction is generally considered to be the most important somatic effect of low doses of ionizing radiation. It is therefore of great concern to assess the quantitative cancer risk of exposure to radiations of different quality and to obtain information on the dose-response relationships for carcinogenesis. Tissues in the human with a high sensitivity for cancer induction include the bone marrow, the lung, the thyroid and the breast in women. If the revised dosimetry estimates for the Japanese survivors of the atomic bomb explosions are correct, there is no useful data base left to derive r.b.e. values for human carcinogenesis. As a consequence, it will be necessary to rely on results obtained in biological systems, including experimental animals, for these estimates. With respect to radiation protection, the following aspects of experimental studies on radiation carcinogenesis are of relevance: Assessment of the nature of dose-response relationships. Determination of the relative biological effectiveness of radiations of different quality. Effects of fractionation or protraction of the dose on tumour development. For the analysis of tumour data in animals, specific approaches have to be applied which correct for competing risks. These methods include actuarial estimates, non-parametric models and analytical models. The dose-response curves for radiation-induced cancers in different tissues vary in shape. This is exemplified by studies on myeloid leukaemia in mice and mammary neoplasms in different rat strains. The results on radiation carcinogenesis in animal models clearly indicate that the highest r.b.e. values are observed for neutrons with energies between 0.5 and 1 MeV. On the basis of such results it might be concluded that the maximum quality factor of 10 for neutrons should be increased. Based on current evidence, an increase by a factor of 2 to 3 seems more realistic than a tenfold rise. The diversity of dose-response relationships point to different mechanisms involved in the induction of different tumours in various species and even in different strains of the same species.     

Factors influencing hydroxylamine inactivation of photosynthetic water oxidation

The kinetics of Mn release during NH₂OH inactivation of the water oxidizing reaction is largely insensitive to the S-state present during addition of NH₂OH. This appears to reflect reduction by NH₂OH of higher S-states to a common more reduced state (S₀ or S_?1) which alone is susceptible to NH₂OH inactivation. Sequences of saturating flashes with dark intervals in the range 0.2–5 s^?1 effectively prevent NH₂OH inactivation and the associated liberation of manganese. This light-induced protection disappears rapidly when the dark interval is longer than about 5 s. Under continuous illumination, protection against NH₂OH inactivation is maximally effective at intensities in the range 10³–10⁴ erg · cm^?2 · s^?1. This behavior differs from that of NH₂OH-induced Mn release, which is strongly inhibited at all intensities greater than 10³ erg · cm^?2 · s^?1. This indicates that two distinct processes are responsible for inactivation of water oxidation at high and low intensities. Higher S-states appear to be immune to the reaction by which NH₂OH liberates manganese, although the overall process of water oxidation is inactivated by NH₂OH in the presence of intense light. The light-induced protection phenomenon is abolished by 50 μM DCMU, but not by high concentrations of carbonyl cyanide m-chlorophenylhydrazone, which accelerates inactivation reactions of the water-splitting enzyme, Y (an ADRY reagent). The latter compound accelerates both inactivation of water oxidation and manganese extraction in the dark.     

Genomic instability, bystander effects and radiation risks: implications for development of protection strategies for man and the environment

The last few years has seen what people are now referring to as a "shifting Paradigm" in our way of thinking about radiation effects on biological systems. The concept of the central role of DNA damage due to double strand breaks induced by a radiation "hit" has been itself hit by many studies showing persistent effects in the distant progeny of radiation exposed cells. This phenomenon is known as radiation induced genomic instability. More recently evidence has been accumulating that not even the parent cell need be exposed to radiation (the bystander effect). The new paradigm suggests that cellular stress responses or damage signalling through a range of signal transduction pathways are involved and that cell-cell contact or secretion of damage signalling molecules can induce responses in undamaged and unirradiated cells. Are these new effects relevant to risk assessment, or does it matter HOW radiation affects cells if we have good epidemiological evidence of which to base our risk estimates? The aim of this paper is to introduce the new concepts and to consider reasons why they might alter our methods of risk estimation. The paper also considers the impact of the new concepts on environmental protection and discusses the need for research in the field of comparative radiobiology if we are to develop policies which can adequately protect biodiversity.     

Factors influencing the microbial composition of metalworking fluids and potential implications for machine operator's lung

Hypersensitivity pneumonitis, also known as "machine operator's lung" (MOL), has been related to microorganisms growing in metalworking fluids (MWFs), especially Mycobacterium immunogenum. We aimed to (i) describe the microbiological contamination of MWFs and (ii) look for chemical, physical, and environmental parameters associated with variations in microbiological profiles. We microbiologically analyzed 180 MWF samples from nonautomotive plants (e.g., screw-machining or metal-cutting plants) in the Franche-Comté region in eastern France and 165 samples from three French automotive plants in which cases of MOL had been proven. Our results revealed two types of microbial biomes: the first was from the nonautomotive industry, showed predominantly Gram-negative rods (GNR), and was associated with a low risk of MOL, and the second came from the automotive industry that was affected by cases of MOL and showed predominantly Gram-positive rods (GPR). Traces of M. immunogenum were sporadically detected in the first type, while it was highly prevalent in the automotive sector, with up to 38% of samples testing positive. The use of chromium, nickel, or iron was associated with growth of Gram-negative rods; conversely, growth of Gram-positive rods was associated with the absence of these metals. Synthetic MWFs were more frequently sterile than emulsions. Vegetable oil-based emulsions were associated with GNR, while mineral ones were associated with GPR. Our results suggest that metal types and the nature of MWF play a part in MWF contamination, and this work shall be followed by further in vitro simulation experiments on the kinetics of microbial populations, focusing on the phenomena of inhibition and synergy.