Diversity of digger wasps in Kasnak Oak Forest Nature Reserve,Isparta, Turkey,with records of eight species new to Turkey (Hymenoptera: Sphecidae,Crabronidae and Ampulicidae)

Three stations selected for altitude and habitat types within the Kasnak Oak Forest Nature Reserve (Isparta, Turkey) were studied comparatively for species composition, richness and abundance of the Hymenoptera families Ampulicidae, Crabronidae, and Sphecidae. A total of 56 species was recorded, 1 from the family Ampulicidae, 43 from the family Crabronidae and 12 from the family Sphecidae. 8 species are new records for Turkey.     

Identifying the sister group to the bees: a molecular phylogeny of Aculeata with an emphasis on the superfamily Apoidea

Debevec, AH., Cardinal, S & Danforth, BN. Identifying the sister group to the bees: a molecular phylogeny of Aculeata with an emphasis on the superfamily Apoidea. —Zoologica Scripta, 41, 527–535. The hymenopteran superfamily Apoidea includes the bees (Anthophila) as well as four predatory wasp families (Heterogynaidae, Ampulicidae, Sphecidae and Crabronidae) collectively referred to as the “sphecoid” or “apoid” wasps. The most widely cited studies suggest that bees are sister to the wasp family Crabronidae, but alternative hypotheses have been proposed based on both morphological and molecular data. We combined DNA sequence data from previously published studies and newly generated data for four nuclear genes (28S, long‐wavelength rhodopsin, elongation factor‐1α and wingless) to identify the likely sister group to the bees. Analysis of our four‐gene data set by maximum likelihood and Bayesian methods indicates that bees most likely arise from within a paraphyletic Crabronidae. Possible sister groups to the bees include Philanthinae, Pemphredoninae or Philanthinae + Pemphredoninae. We used Bayesian methods to explore the robustness of our results. Bayes Factor tests strongly rejected the hypotheses of crabronid monophyly as well as placement of Heterogynaidae within Crabronidae. Our results were also stable to alternative rootings of the bees. These findings provide additional support for the hypothesis that bees arise from within Crabronidae, rather than being sister to Crabronidae, thus altering our understanding of bee ancestry and evolutionary history.     

A cladistic analysis of the cockroach wasps based on morphological data (Hymenoptera: Ampulicidae)

The Ampulicidae are one of the most basal groups within the apoid wasps, the paraphyletic assemblage of wasps previously known as Sphecidae. The morphology and taxonomy of the Ampulicidae are poorly studied, and there is no strict cladistic approach on their phylogeny yet. Here we assemble morphological characters of phylogenetic significance and submit them to parsimony analyses using modern cladistic methods. This produces a well-resolved and firmly supported phylogenetic hypothesis on the generic relationships within the group. Based on our phylogenetic results a revised classification is provided, subdividing the Ampulicidae into two monophyletic subfamilies, Ampulicinae ( Ampulex and Trirogma ) and Dolichurinae, the latter comprising two monophyletic tribes, Dolichurini ( Dolichurus and Paradolichurus ) and Aphelotomini, new tribe ( Aphelotoma and Riekefella ). Based on the resulting cladogram, the historical biogeography and the fossil record of Ampulicidae are briefly discussed.
© The Willi Hennig Society 2009.     

A preliminary detective survey of hymenopteran insects at Jazan Lake Dam Region,Southwest of Saudi Arabia

A preliminary detective survey for the hymenopteran insect fauna of Jazan Lake dam region, Southwest Saudi Arabia, was carried out for one year from January 2018 to January 2019 using mainly sweep nets and Malaise traps. The survey revealed the presence of three hymenopteran Superfamilies (Apoidea, Vespoidea and Evanioidea) representing 15 species belonging to 10 genera of 6 families (Apidae, Crabronidae, Sphecidae, Vespidae, Mutillidae, and Evaniidae). The largest number of species has belonged to the family Crabronidae is represented by 6 species under 2 genera. While the family Apidae, is represented by 2 species under 2 genera. Family Vespidae is represented by 2 species of one genus. While, the rest of the families Sphecidae, Mutillida, and Evaniidae each is represented by only one species and one genus each. Eleven species are predators, two species are pollinators and two species are parasitics. Note for each family was provided, and species was provided with synonyms and general and taxonomic remarks and their worldwide geographic distribution and information about their economic importance are also included. All species were photographed with dorsal and lateral views.     

DNA barcoding largely supports 250 years of classical taxonomy: identifications for Central European bees (Hymenoptera,Apoidea partim)

This study presents DNA barcode records for 4118 specimens representing 561 species of bees belonging to the six families of Apoidea (Andrenidae, Apidae, Colletidae, Halictidae, Megachilidae and Melittidae) found in Central Europe. These records provide fully compliant barcode sequences for 503 of the 571 bee species in the German fauna and partial sequences for 43 more. The barcode results are largely congruent with traditional taxonomy as only five closely allied pairs of species could not be discriminated by barcodes. As well, 90% of the species possessed sufficiently deep sequence divergence to be assigned to a different Barcode Index Number (BIN). In fact, 56 species (11%) were assigned to two or more BINs reflecting the high levels of intraspecific divergence among their component specimens. Fifty other species (9.7%) shared the same Barcode Index Number with one or more species, but most of these species belonged to a distinct barcode cluster within a particular BIN. The barcode data contributed to clarifying the status of nearly half the examined taxonomically problematic species of bees in the German fauna. Based on these results, the role of DNA barcoding as a tool for current and future taxonomic work is discussed.     

A molecular phylogeny for digger wasps in the tribe Ammophilini (Hymenoptera,Apoidea, Sphecidae)

The evolution of parental care strategies in aculeate (stinging) wasps and bees has been much studied from a functional perspective, but relatively little phylogenetic information is available to place this in a rigorous historical context, especially at the species level. We used mitochondrial cytochrome oxidase I and two nuclear genes, the elongation factor‐1α and LW rhodopsin, to investigate the phylogeny of Sphecidae digger wasps. We focus particularly on the tribe Ammophilini, a clade of nonsocial apoid wasps that exhibit unusually diverse parental care strategies. We analysed a 2232 bp dataset for 40 ammophilines plus nine other taxa from within the remaining Sphecidae. Our Bayesian phylogeny provides strong support for the monophyly of Ammophilini and for the monophyly of all six individual ammophiline genera, except that the position of P. affinis within the genus Podalonia is only weakly supported. The monophyly of some, but not all, previously designated species groups within the genus Ammophila is supported. We discuss the implications of our results for the evolution of morphological traits used previously in ammophiline systematics.     

A comprehensive DNA barcode database for Central European beetles with a focus on Germany: adding more than 3500 identified species to BOLD

Beetles are the most diverse group of animals and are crucial for ecosystem functioning. In many countries, they are well established for environmental impact assessment, but even in the well‐studied Central European fauna, species identification can be very difficult. A comprehensive and taxonomically well‐curated DNA barcode library could remedy this deficit and could also link hundreds of years of traditional knowledge with next generation sequencing technology. However, such a beetle library is missing to date. This study provides the globally largest DNA barcode reference library for Coleoptera for 15 948 individuals belonging to 3514 well‐identified species (53% of the German fauna) with representatives from 97 of 103 families (94%). This study is the first comprehensive regional test of the efficiency of DNA barcoding for beetles with a focus on Germany. Sequences ≥500 bp were recovered from 63% of the specimens analysed (15 948 of 25 294) with short sequences from another 997 specimens. Whereas most specimens (92.2%) could be unambiguously assigned to a single known species by sequence diversity at CO1, 1089 specimens (6.8%) were assigned to more than one Barcode Index Number (BIN), creating 395 BINs which need further study to ascertain if they represent cryptic species, mitochondrial introgression, or simply regional variation in widespread species. We found 409 specimens (2.6%) that shared a BIN assignment with another species, most involving a pair of closely allied species as 43 BINs were involved. Most of these taxa were separated by barcodes although sequence divergences were low. Only 155 specimens (0.97%) show identical or overlapping clusters.     

Digger wasps (Hymenoptera,Apoidea: Ampulicidae,Sphecidae, Crabronidae) of the Black Sea coast of Krasnodar Territory,Abkhazia, and adjacent areas

A list of 208 digger-wasp species (Hymenoptera, Apoidea: Ampulicidae, Sphecidae, Crabronidae) from the Black Sea coast and adjacent areas within Krasnodar Territory, Abkhazia, and Adygea is given. Gorytes schmiedeknechti Handlirsch, 1888, Miscophus albufeirae Andrade, 1952, Psenulus fulvicornis (Schenck, 1857), and Passaloecus pictus Ribaut, 1952) are new to the Russian fauna, and 47 species are recorded for the first time from Abkhazia. Ectemnius guttatus (Vander Linden, 1829) does not occur in the Western Caucasus; the record of the species from this region was based on a misidentified female of E. lituratus Pz. Tachysphex gibbus Kohl, 1885, stat. n. is considered a distinct species based on its ecological and morphological features.     

DNA barcoding of crickets,katydids and grasshoppers (Orthoptera) from Central Europe with focus on Austria,Germany and Switzerland

We present a DNA barcoding study on the insect order Orthoptera that was generated in collaboration between four barcoding projects in three countries, viz. Barcoding Fauna Bavarica (Germany), German Barcode of Life, Austrian Barcode of Life and Swiss Barcode of Life. Our data set includes 748 COI sequences from 127 of the 162 taxa (78.4%) recorded in the three countries involved. Ninety‐three of these 122 species (76.2%, including all Ensifera) can be reliably identified using DNA barcodes. The remaining 26 caeliferan species (families Acrididae and Tetrigidae) form ten clusters that share barcodes among up to five species, in three cases even across different genera, and in six cases even sharing individual barcodes. We discuss incomplete lineage sorting and hybridization as most likely causes of this phenomenon, as the species concerned are phylogenetically young and hybridization has been previously observed. We also highlight the problem of nuclear mitochondrial pseudogenes (numts), a known problem in the barcoding of orthopteran species, and the possibility of Wolbachia infections. Finally, we discuss the possible taxonomic implications of our barcoding results and point out future research directions.     

DNA Barcoding for Species Assignment: The Case of Mediterranean Marine Fishes

Background

DNA barcoding enhances the prospects for species-level identifications globally using a standardized and authenticated DNA-based approach. Reference libraries comprising validated DNA barcodes (COI) constitute robust datasets for testing query sequences, providing considerable utility to identify marine fish and other organisms. Here we test the feasibility of using DNA barcoding to assign species to tissue samples from fish collected in the central Mediterranean Sea, a major contributor to the European marine ichthyofaunal diversity.

Methodology/Principal Findings

A dataset of 1278 DNA barcodes, representing 218 marine fish species, was used to test the utility of DNA barcodes to assign species from query sequences. We tested query sequences against 1) a reference library of ranked DNA barcodes from the neighbouring North East Atlantic, and 2) the public databases BOLD and GenBank. In the first case, a reference library comprising DNA barcodes with reliability grades for 146 fish species was used as diagnostic dataset to screen 486 query DNA sequences from fish specimens collected in the central basin of the Mediterranean Sea. Of all query sequences suitable for comparisons 98% were unambiguously confirmed through complete match with reference DNA barcodes. In the second case, it was possible to assign species to 83% (BOLD-IDS) and 72% (GenBank) of the sequences from the Mediterranean. Relatively high intraspecific genetic distances were found in 7 species (2.2%–18.74%), most of them of high commercial relevance, suggesting possible cryptic species.

Conclusion/Significance

We emphasize the discriminatory power of COI barcodes and their application to cases requiring species level resolution starting from query sequences. Results highlight the value of public reference libraries of reliability grade-annotated DNA barcodes, to identify species from different geographical origins. The ability to assign species with high precision from DNA samples of disparate quality and origin has major utility in several fields, from fisheries and conservation programs to control of fish products authenticity.     

Efficacy of using DNA barcoding to identify parasitoid wasps of the melon-cotton aphid (<Emphasis Type="Italic">Aphis gossypii</Emphasis>) in watermelon cropping system

Parasitoid wasps have received a great deal of attention in the biological control of melon-cotton aphid (Aphis gossypii Glover). The species of parasitoids are often difficult to identify because of their small body size and profound diversity. DNA barcoding offers scientists who are not expert taxonomists a powerful tool to render their field studies more accurate. Using DNA barcodes to identify aphid parasitoid wasps in specific cropping systems may provide valuable information for biological control. Here, we report the use of DNA barcoding to confirm the morphological identification of 14 species (belonging to 13 genera of 7 families) of parasitoid wasps from two-year field samples in a watermelon cropping system. We generated DNA sequences from the mitochondrial COI gene and the nuclear D2 region of 28S rDNA to assess the genetic variation within and between parasitoid species. Automatic Barcode Gap Discovery (ABGD) supported the presence of 14 genetically distinct groups in the dataset. Among the COI sequences, we found no overlap between the maximum K2P distance within species (0.49%) and minimum distance between species (6.85%). The 28S sequences also showed greater interspecific distance than intraspecific distance. DNA barcoding confirmed the morphological identification. However, inconsistency and ambiguity of taxonomic information available in the online databases has limited the successful use of DNA barcoding. Only five species matched those in the BOLD and GenBank. Four species did not match the entries in GenBank and five species showed ambiguous results in BOLD due to confusing nomenclature. We suggested that species identification based on DNA barcodes should be performed using both COI and other genes. Nonetheless, we demonstrate the potential of the DNA barcoding approach to confirm field identifications and to provide a foundation for studies aimed at improving the understanding of the biocontrol services provided by parasitoids in the melon ecosystem.     

The phylogenetic position of the enigmatic wasp family Heterogynaidae based on molecular data, with description of a new, nocturnal species (Hymenoptera: Apoidea)

Abstract.  The wasp family Heterogynaidae is described and previous phylogenetic hypotheses on its position within the Apoidea are discussed. The higher level phylogeny of the Apoidea based on sequence data of a nuclear gene, long-wavelength (LW) opsin, is analysed for the first time. Although preliminary because of limited taxon sampling and use of a single gene, the analysis reveals some significant results. Monophyly of the Apoidea, including the Heterogynaidae, is supported strongly, as is monophyly of Heterogynaidae. The monophyly of the bees is also confirmed, but support is rather weak. Monophyly of the Ampulicidae + Sphecidae, which is the sister group to the remaining Apoidea (Crabronidae + Anthophila + Heterogynaidae), is confirmed. Our results imply that Sphecidae may be paraphyletic with respect to Ampulicidae, and Crabronidae with respect to bees and Heterogynaidae. Remarkably, Heterogynaidae is well nested within the Crabronidae + Anthophila clade, which is in conflict with the morphological data. This result implies that Heterogynaidae may not have originated from a basal node within Apoidea, as previously considered, and that the character states presumed to be plesiomorphies may instead be homoplasies. Our analysis confirms the value of LW opsin in resolving deeper nodes within Apoidea. A new species, Heterogyna nocticola Ohl, from the Sultanate of Oman is described based on both sexes, with a diagnosis, information on distribution and collecting period. A key to the Palaearctic species of Heterogynaidae is presented.     

Alpha–beta diversity in trap-nesting wasps (Hymenoptera: Aculeata) in Southern Brazil

This study was carried out at the Parque Municipal das Araucárias, in the municipality of Guarapuava, state of Paraná, southern Brazil. The aims of the present study were to access the spectrum of wasp species inhabiting trap-nests in three habitats, to record their richness and diversity and elucidate the process responsible for their composition and abundance and to investigate if there is difference in their diversity between Brazilian tropical and subtropical regions. A total of 1097 nests, belonging to 21 species of solitary wasps, were collected. Of these, nine belonged to the family Vespidae (42.86%), six to Pompilidae (28.57%), four to Crabronidae (19.05%) and two to Sphecidae (9.52%). Crabronidae was the most abundant family in the samplings, with a total of 927 collected nests (84.3%). The environment that presented the greatest species diversity, richness and evenness was the grassland areas. Using the Bray–Curtis coefficient (beta diversity), the greatest similarity, in relation to species abundance, occurred between swamp and grasslands. In contrast, the araucaria forest was the habitat with the greatest dissimilarity, a result corroborated by the correspondence analysis.     

Taxonomic challenges in freshwater fishes: a mismatch between morphology and DNA barcoding in fish of the north‐eastern part of the Congo basin

This study evaluates the utility of DNA barcoding to traditional morphology‐based species identifications for the fish fauna of the north‐eastern Congo basin. We compared DNA sequences (COI) of 821 samples from 206 morphologically identified species. Best match, best close match and all species barcoding analyses resulted in a rather low identification success of 87.5%, 84.5% and 64.1%, respectively. The ratio ‘nearest‐neighbour distance/maximum intraspecific divergence’ was lower than 1 for 26.1% of the samples, indicating possible taxonomic problems. In ten genera, belonging to six families, the number of species inferred from mtDNA data exceeded the number of species identified using morphological features; and in four cases indications of possible synonymy were detected. Finally, the DNA barcodes confirmed previously known identification problems within certain genera of the Clariidae, Cyprinidae and Mormyridae. Our results underscore the large number of taxonomic problems lingering in the taxonomy of the fish fauna of the Congo basin and illustrate why DNA barcodes will contribute to future efforts to compile a reliable taxonomic inventory of the Congo basin fish fauna. Therefore, the obtained barcodes were deposited in the reference barcode library of the Barcode of Life Initiative.     

A DNA barcode library for 5,200 German flies and midges (Insecta: Diptera) and its implications for metabarcoding‐based biomonitoring

This study summarizes results of a DNA barcoding campaign on German Diptera, involving analysis of 45,040 specimens. The resultant DNA barcode library includes records for 2,453 named species comprising a total of 5,200 barcode index numbers (BINs), including 2,700 COI haplotype clusters without species‐level assignment, so called “dark taxa.” Overall, 88 out of 117 families (75%) recorded from Germany were covered, representing more than 50% of the 9,544 known species of German Diptera. Until now, most of these families, especially the most diverse, have been taxonomically inaccessible. By contrast, within a few years this study provided an intermediate taxonomic system for half of the German Dipteran fauna, which will provide a useful foundation for subsequent detailed, integrative taxonomic studies. Using DNA extracts derived from bulk collections made by Malaise traps, we further demonstrate that species delineation using BINs and operational taxonomic units (OTUs) constitutes an effective method for biodiversity studies using DNA metabarcoding. As the reference libraries continue to grow, and gaps in the species catalogue are filled, BIN lists assembled by metabarcoding will provide greater taxonomic resolution. The present study has three main goals: (a) to provide a DNA barcode library for 5,200 BINs of Diptera; (b) to demonstrate, based on the example of bulk extractions from a Malaise trap experiment, that DNA barcode clusters, labelled with globally unique identifiers (such as OTUs and/or BINs), provide a pragmatic, accurate solution to the “taxonomic impediment”; and (c) to demonstrate that interim names based on BINs and OTUs obtained through metabarcoding provide an effective method for studies on species‐rich groups that are usually neglected in biodiversity research projects because of their unresolved taxonomy.     

Sublethal effect of brood parasitism on the grass-carrying wasp Isodontia mexicana

Abstract.  1. The nests of solitary, nest-provisioning wasps (Sphecidae and Crabronidae) are commonly attacked by brood parasites, including flies of the families Phoridae and Sarcophagidae. Larvae of the flies commonly kill the wasp offspring directly or starve it by consuming prey provided by the adult female wasps.
2. To determine whether brood parasitic flies can have a sublethal effect (i.e. reduced body size) on wasp offspring, nests of the wasp Isodontia mexicana were collected at two field sites in upstate New York, U.S.A. Nest diameter had no effect on the probability that a wasp cell would be inhabited by a brood parasite. Most offspring that developed in cells also containing phorids or sarcophagids managed to complete development and emerge as adults. Nevertheless, they had significantly smaller body size than conspecifics emerging from unparasitised cells in which the developing wasp did not have to compete for food (which in this species consists of tree crickets and katydids). Apparently, this is the first time that a sublethal effect of brood parasitism on offspring body size has been quantified for a solitary wasp species. Known effects of body size on the reproductive success of adult wasps suggest that sublethal consequences of the presence of brood parasites may have a significant effect on the fitness of adult I. mexicana .     

The dark side of pseudoscorpion diversity: The German Barcode of Life campaign reveals high levels of undocumented diversity in European false scorpions

DNA barcoding is particularly useful for identification and species delimitation in taxa with conserved morphology. Pseudoscorpions are arachnids with high prevalence of morphological crypsis. Here, we present the first comprehensive DNA barcode library for Central European Pseudoscorpiones, covering 70% of the German pseudoscorpion fauna (35 out of 50 species). For 21 species, we provide the first publicly available COI barcodes, including the rare Anthrenochernes stellae Lohmander, a species protected by the FFH Habitats Directive. The pattern of intraspecific COI variation and interspecific COI variation (i.e., presence of a barcode gap) generally allows application of the DNA barcoding approach, but revision of current taxonomic designations is indicated in several taxa. Sequences of 36 morphospecies were assigned to 74 BINs (barcode index numbers). This unusually high number of intraspecific BINs can be explained by the presence of overlooked cryptic species and by the accelerated substitution rate in the mitochondrial genome of pseudoscorpions, as known from previous studies. Therefore, BINs may not be an appropriate proxy for species numbers in pseudoscorpions, while partitions built with the ASAP algorithm (Assemble Species by Automatic Partitioning) correspond well with putative species. ASAP delineated 51 taxonomic units from our data, an increase of 42% compared with the present taxonomy. The Neobisium carcionoides complex, currently considered a polymorphic species, represents an outstanding example of cryptic diversity: 154 sequences from our dataset were allocated to 23 BINs and 12 ASAP units.     

Recovery of DNA barcodes from blackfly museum specimens (Diptera: Simuliidae) using primer sets that target a variety of sequence lengths

In this study, we evaluated the efficacy of various primers for the purpose of DNA barcoding old, pinned museum specimens of blackflies (Diptera: Simuliidae). We analysed 271 pinned specimens representing two genera and at least 36 species. Due to the age of our material, we targeted overlapping DNA fragments ranging in size from 94 to 407 bp. We were able to recover valid sequences from 215 specimens, of which 18% had 500‐ to 658‐bp barcodes, 36% had 201‐ to 499‐bp barcodes and 46% had 65‐ to 200‐bp barcodes. Our study demonstrates the importance of choosing suitable primers when dealing with older specimens and shows that even very short sequences can be diagnostically informative provided that an appropriate gene region is used. Our study also highlights the lack of knowledge surrounding blackfly taxonomy, and we briefly discuss the need for further phylogenetic studies in this socioeconomically important family of insects.     

DNA barcoding the geometrid fauna of Bavaria (Lepidoptera): successes, surprises, and questions

Background

The State of Bavaria is involved in a research program that will lead to the construction of a DNA barcode library for all animal species within its territorial boundaries. The present study provides a comprehensive DNA barcode library for the Geometridae, one of the most diverse of insect families.

Methodology/Principal Findings

This study reports DNA barcodes for 400 Bavarian geometrid species, 98 per cent of the known fauna, and approximately one per cent of all Bavarian animal species. Although 98.5% of these species possess diagnostic barcode sequences in Bavaria, records from neighbouring countries suggest that species-level resolution may be compromised in up to 3.5% of cases. All taxa which apparently share barcodes are discussed in detail. One case of modest divergence (1.4%) revealed a species overlooked by the current taxonomic system: Eupithecia goossensiata Mabille, 1869 stat.n. is raised from synonymy with Eupithecia absinthiata (Clerck, 1759) to species rank. Deep intraspecific sequence divergences (>2%) were detected in 20 traditionally recognized species.

Conclusions/Significance

The study emphasizes the effectiveness of DNA barcoding as a tool for monitoring biodiversity. Open access is provided to a data set that includes records for 1,395 geometrid specimens (331 species) from Bavaria, with 69 additional species from neighbouring regions. Taxa with deep intraspecific sequence divergences are undergoing more detailed analysis to ascertain if they represent cases of cryptic diversity.     

西双版纳不同经济作物环境泥蜂物种多样性研究

泥蜂是一类重要的捕猎性天敌昆虫和传粉昆虫。本研究于2018年6月-2019年11月对西双版纳地区观赏经作区、柚林区、古茶林区和农田区(样地Ⅰ-Ⅳ)4个调查区采用马氏网诱捕方法定期定点调查,每月调查一次。结果如下：西双版纳调查区域分布有泥蜂类群3科15族27属110种;其中,泥蜂科包括3族5属7种;蠊泥蜂科包括2族3属8种;方头泥蜂科包括10族19属95种,为该地区的优势科。脊短柄泥蜂属Psenulus、小唇泥蜂属Larra和短翅泥蜂属Trypoxylon为优势属,刻臀小唇泥蜂Larra fenchihuensis、磨光小唇泥蜂红腿亚种Larra polita luzonensis为优势种。4个作物区的优势科均为方头泥蜂科,但不同作物区泥蜂的优势属种存在差异,观赏经作区(样地Ⅰ)优势属为脊短柄泥蜂属和短翅泥蜂属,优势种为普氏脊短柄泥蜂指名亚种Psenulus pulcherrimus pulcherrimus(17.4%)和岩田隆痣短柄泥蜂Carinostigmus iwatai(15.6%);柚木林区(样地Ⅱ)优势属为脊短柄泥蜂属和短翅泥蜂属,优势种为普氏脊短柄泥蜂指名亚种(17.7%...