共查询到20条相似文献,搜索用时 15 毫秒
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Murray R Boase David H Lewis Kevin M Davies Gayle B Marshall Deepa Patel Kathy E Schwinn Simon C Deroles 《BMC plant biology》2010,10(1):107
Background
Cyclamen is a popular and economically significant pot plant crop in several countries. Molecular breeding technologies provide opportunities to metabolically engineer the well-characterized flavonoid biosynthetic pathway for altered anthocyanin profile and hence the colour of the flower. Previously we reported on a genetic transformation system for cyclamen. Our aim in this study was to change pigment profiles and flower colours in cyclamen through the suppression of flavonoid 3', 5'-hydroxylase, an enzyme in the flavonoid pathway that plays a determining role in the colour of anthocyanin pigments. 相似文献15.
Molecular genetics of blood‐fleshed peach reveals activation of anthocyanin biosynthesis by NAC transcription factors
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Hui Zhou Kui Lin‐Wang Huiliang Wang Chao Gu Andrew P. Dare Richard V. Espley Huaping He Andrew C. Allan Yuepeng Han 《The Plant journal : for cell and molecular biology》2015,82(1):105-121
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Park JS Choung MG Kim JB Hahn BS Kim JB Bae SC Roh KH Kim YH Cheon CI Sung MK Cho KJ 《Plant cell reports》2007,26(4):507-516
Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method.
BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in
secondary metabolism. Such clones had a particularly high abundance of flavonoid-metabolism proteins (6.5%). Following SSH,
566 clones were rescreened for differential gene expression using dot-blot hybridization. Of these, 53 were found to overexpressed
during red coloration. The up-regulated expression of six genes was confirmed by Northern blot analyses. The expression of
chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and dihydroflavonol 4-reductase (DFR) genes showed a positive correlation with anthocyanin accumulation in UV-B-irradiated lettuce leaves; flavonoid 3′,5′-hydroxylase (F3′,5′H) and anthocyanidin synthase (ANS) were expressed continuously in both samples. These results indicated that the genes CHS, F3H, and DFR coincided with increases
in anthocyanin accumulation during the red coloration of lettuce leaves. This study show a relationship between red coloration
and the expression of up-regulated genes in lettuce. The subtractive cDNA library and EST database described in this study
represent a valuable resource for further research for secondary metabolism in the vegetable crops. 相似文献
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The proanthocyanidin‐specific transcription factor MdMYBPA1 initiates anthocyanin synthesis under low‐temperature conditions in red‐fleshed apples
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Nan Wang Changzhi Qu Shenghui Jiang Zijing Chen Haifeng Xu Hongcheng Fang Mengyu Su Jing Zhang Yicheng Wang Wenjun Liu Zongying Zhang Ninglin Lu Xuesen Chen 《The Plant journal : for cell and molecular biology》2018,96(1):39-55
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Jean Baptiste Bassene Yann Froelicher Claudie Dhuique-Mayer Waffa Mouhaya Rosa Mar Ferrer Gema Ancillo Raphael Morillon Luis Navarro Patrick Ollitrault 《Plant cell reports》2009,28(11):1689-1697
Allopolyploidy is known to induce novel patterns of gene expression and often gives rise to new phenotypes. Here we report
on the first attempt to relate phenotypic inheritance in an allotetraploid somatic hybrid with gene expression. Carotenoid
compounds in the fruit pulp of the two parental species and the hybrid were evaluated quantitatively by HPLC. Only very low
levels of β-carotene and β-cryptoxanthin were observed in Citrus limon, while β-cryptoxanthin was a major component of C. reticulata, which also displayed high levels of phytoene, phytofluene, β-carotene, lutein, zeaxantin and violaxanthin. Total carotenoid
content in mandarin juice sacs was 60 times greater than that in lemon. The allotetraploid hybrid produced all the same compounds
as mandarin but at very low levels. Transgressive concentration of abscisic acid (ABA) was observed in the somatic hybrid.
Real-time RT-PCR of total RNA from juice sacs was used to study expression of seven genes (CitDxs, CitPsy, CitPds, CitZds, CitLcy-b, CitChx-b, and CitZep) of the carotenoid biosynthetic pathway and two genes (CitNced1 and CitNced2) involved in abscisic acid synthesis from carotenoid. Gene expression was significantly higher for mandarin than lemon for
seven of the nine genes analyzed. Lemon under expression was partially dominant in the somatic hybrid for three upstream steps
of the biosynthetic pathway, particularly for CitDxs. Transgressive over expression was observed for the two CitNced genes. A limitation of the upstream steps of the pathway and a downstream higher consumption of carotenoids may explain the
phenotype of the somatic hybrid. 相似文献