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1.
《Micron and Microscopica Acta》1986,17(2):149-166
The subsequent development and application of the negative staining technique to isolated virus particles and their components, following the early studies on the structure of T2 bacteriophage proteins, is described. The use of the method to prepare particles covering a wide range of different types of virus for examination in the electron microscope is reviewed, together with more recent advances in image analysis of electron micrographs obtained from negatively stained virus specimens. 相似文献
2.
An improved negative staining technique using a thin quartz membrane as sample support 总被引:1,自引:0,他引:1
A negative staining technique is presented based on the use of 40-60 nm quartz membrane supported by a silicon grid. The quartz membrane is fabricated by thermal growth of silicon dioxide on a silicon substrate followed by an anisotropic silicon etching step giving rectangular holes in the silicon substrate. The hydrophilic membrane is shown to be ideally suited for negative staining due to its spreading characteristics, homogeneity, heat resistance and mechanical stability. Micrographs of phage lambda are presented showing the detailed structure of the tail. A simple method of calculating the number of adsorbed particles based on diffusion limited association is also presented. 相似文献
3.
A negative staining technique is presented baaed on the use of 40-60 nm quartz membrane supported by a silicon grid. The quartz membrane is fabricated by thermal growth of silicon dioxide on a silicon substrate followed by an anisotropic silicon etching step giving rectangular holes in the silicon substrate. The hydrophilic membrane is shown to be ideally suited for negative staining due to its spreading characteristics, homogeneity, heat resistance and mechanical stability. Micrographs of phage λ are presented showing the detailed structure of the tail. A simple method of calculating the number of adsorbed particles based on diffusion limited association is also presented. 相似文献
4.
June D. Almeida 《CMAJ》1963,89(16):787-798
Recent improvements in electron microscope techniques which allow the study of virus fine structure have permitted the grouping of many viruses on a purely morphological basis. Briefly the techniques used in electron microscopy for the study of viruses are reviewed and the symmetry properties of virus particles as revealed by negative staining are discussed somewhat more fully.Finally, virus particles are grouped on two bases, firstly the site of formation of the virus within the cell as seen by thin sectioning techniques, and secondly the symmetry property of the virus as seen by negative staining. Consideration of the groupings obtained in this way reveals that the biochemical and physical properties of a virus can be deduced from the readily established morphological characteristics. 相似文献
5.
Usefulness of estrogen receptor detection using archival Papanicolaou-stained smears. 总被引:1,自引:0,他引:1
OBJECTIVE: To examine estrogen receptor (ER) detection using cytologic specimens and to compare the results with those obtained by the dextran-coated charcoal (DCC) method and enzyme immunoassay (EIA). STUDY DESIGN: Immunocytochemical staining was conducted on 60 cases of breast cancer resected at our hospital between April 1993 and November 1997 in which ER had been measured by DCC or EIA. Specimens for immunocytochemical staining were prepared by a cell transfer method using archival Papanicolaou-stained imprint smears, and ER staining was performed by the labeled streptavidin method using an anti-ER monoclonal antibody. These results were compared with those obtained by DCC or EIA. RESULTS: In immunocytochemical staining for ER, positive staining was observed in the nuclei of tumor cells. A good correlation was obtained between the immunocytochemical staining results and biochemical results. Five cases were positive in anti-ER staining but negative in biochemical tests, and two cases were negative in anti-ER staining and positive in biochemical tests. CONCLUSION: Unlike biochemical assays, the immunocytochemical method does not necessitate use of fresh frozen materials and can be performed even using archival Papanicolaou-stained smears. Immunocytochemical study is a highly useful method for routine ER determination. 相似文献
6.
J. D. Almeida 《The Yale journal of biology and medicine》1980,53(1):5-18
The electron microscope technique of negative staining was first used to obtain fundamental information about virus morphology, but in recent years it has developed into a practical method for virus diagnosis. The methods employed are both simple and rapid. The following review discusses in detail the steps that must be taken to obtain good electron microscope preparations and illustrates some of the results that can be obtained. 相似文献
7.
Micro-neutralization test for mumps virus using the 96-well tissue culture plate and PAP (peroxidase-antiperoxidase) staining technique 总被引:2,自引:0,他引:2
A rapid micro-test method for mumps virus neutralization was developed. In this method, a 96-well tissue culture plate was used for preparation of cell monolayers and the PAP staining technique was used for visualization of mumps virus infected cells. Clusters of infected cells were observed as a focus and the numbers of foci could be counted by the naked eye 2 days after the infection. A linear relationship between virus dilutions and focus numbers was observed. When neutralizing antibodies in sera from cases of natural mumps infection were assayed, a good correlation was observed between those obtained by the focus reduction method applying the micromethod and those obtained by the ordinary plaque method. Our results indicate that this micromethod is useful in mumps virus neutralization tests and it has many advantages over other methods previously reported. 相似文献
8.
用细胞病变阳性(positive cytopathogenic effect,CPE+ )病毒马立克氏病毒(Marek'sdisease virus, MDV)血清1,2,3 型以及细胞病变阴性(negative cytopathogenic effect,CPE- )病毒猪瘟病毒(Hog cholera virus,HCV)强毒与弱毒和鸡新城疫病毒(New castle diseasevirus. NDV)Lasota 毒株及其对应的异硫氢酸荧光素(FITC)标记的特异抗体为试验材料,以免疫荧光抗体技术(FA)为基础、并加以改进,建立了标记抗体染色病毒空斑计数技术. 该技术不仅能克服常规病毒空斑计数技术不能计数细胞病变阴性病毒和一种样品含有两种或两种以上病毒的各自空斑数的缺点,能迅速准确计数出CPE- 病毒和多病毒样品中病毒各自空斑数及其空斑总数,具较高敏感性、良好的可重复性. 相似文献
9.
Direct visualization of the RNA location in cucumber green mottle mosaic virus and tobacco mosaic virus protein disk 总被引:1,自引:0,他引:1
The location of RNA in cucumber green mottle mosaic virus and tobacco mosaic virus protein disks was visualized by a negative staining method as a narrow ring localized at a radius of 4 nm, which corresponds to the location of RNA obtained by X-ray diffraction studies of tobacco mosaic virus. The same ring-shaped stains were observed in the end views of helical rods prepared in acidic solutions from viral protein without RNA. Since such a ring-shaped image could not be observed in end views of natural particles and reconstituted particles composed of protein and RNA, the narrow ring was concluded to indicate the RNA location on the basis of X-ray analysis. 相似文献
10.
《Micron and Microscopica Acta》1991,22(4):449-460
Viruses have unique morphology and are therefore good candidates for negative staining. Negative staining with phosphotungstic acid (PTA) or uranyl acetate has facilitated the detection of many viruses in clinical specimens. Enhancement procedures have included the use of centrifugation and agar diffusion for concentrating virus particles, the use of solid phase capture reagents to trap virus particles and the use of secondary antibodies and electron dense markers to help visualize them. Techniques currently in use and employing negative staining include direct EM, immune electron microscopy (IEM), solid phase immune electron microscopy (SPIEM), colloidal gold-labeled protein A (PAG), solid phase IEM employing a second decorator antibody (SPIEMDAT), and solid phase IEM using colloided gold-labeled secondary antibodies (SPEIMDAGT). IEM methods assist with the detection of small viruses or viruses present in low numbers while PAG offers increased sensitivity over direct EM and IEM. In our experience the serum-in-agar (SIA) method is the most sensitive of the PAG IEM techniques for detection of rotavirus particles in clinical specimens. SPIEMDAT enhances the detection of small viruses which are often missed by other techniques due to background staining in specimens. SPEIMDAGT employing colloidal gold-labeled secondary antibody has increased sensitivity and offers the advantage of detecting viral antigen when whole virus particles are not visible. IEM techniques have recently been used for typing viruses using either monospecific antisera or monoclonal antibodies and colloidal gold-labeled secondary antibody. 相似文献
11.
A P Ponomarev A I Molchanova V L Uziumov 《Nauchnye doklady vysshe? shkoly. Biologicheskie nauki》1984,(6):18-23
It has been shown that the electron microscopy method can be used for characteristics of the electric properties of foot-and-mouth disease virus. The appearance of simultaneous positive and negative staining during the negative staining of virus preparations with 3-4% PTV solution shows the presence of full virions of constant poles designated as positively and negatively stained areas of protein coat surface. The lateral orientation of virions on the film at routine conditions of preparation and the possibility of virion orientation on the film in the external electrical field allow to characterize the full virions as electric dipoles. It has been suggested that there is a relationship between a virus structure and the character of its electric properties. 相似文献
12.
Jacques G. Bindels Huub N.M.W. Nievelstein Herman J. Hoenders 《Analytical biochemistry》1983,131(2):291-294
A more informative method for the visualization of proteins on thin-layer gels, based on combining the images of the gel at different stages of destaining, is presented. It is useful whenever important information seems to be lost after prolonged destaining. The method, which makes it unnecessary to run different loads in different channels, has been developed utilizing isoelectric focusing on polyester film-bound agarose gels. The strongly destained gel is superimposed on a negative image of the same gel made at an earlier phase of destaining, thus showing white spots on a gray background for minor components and dark bands in a white field surrounded by the grey background for the abundant ones. In general, the method may be applied to gel images obtained by different staining procedures. 相似文献
13.
BACKGROUND: Although there is a growing need in the field of biotechnology to rapidly and accurately quantify viruses, time-consuming techniques such as the plaque titer method remain the "gold standard." Flow cytometric methods for virus quantification offer the advantages of rapid analysis and statistical treatment. The technique presented in this work represents the first demonstration of a flow cytometric determination of a viral count that is directly related to the count obtained by plaque titer. METHODS: A flow cytometric instrument for rapid quantification of virus particles was designed, constructed, and thoroughly characterized. A two-color method, which involved staining the viral genome and the protein coat for baculoviruses, was developed in addition to an algorithm to identify simultaneous events on the DNA and protein channels. RESULTS: The instrument was fully characterized, which included analysis of the data acquisition rate, sampling time, flow rate, detection efficiency, linear dynamic range, channel cross-talk, and the limit of detection. Baculovirus samples were analyzed and the results were compared with concentrations obtained by a one-channel flow cytometer and plaque assay. CONCLUSIONS: The dual channel virus counter yields a representative value for the concentration of active viruses in an unpurified sample when compared with plaque assay and a one-channel flow cytometer. The technique is rapid (within minutes), requires only minimal sample preparation and minimum sample size (approximately 100 microl). 相似文献
14.
《Micron (1969)》1981,12(1):37-45
The formation of two-dimensional arrays of isometric plant viruses using the negative staining carbon-film technique has been extended to include experiments on the addition of polyethylene glycol (PEG). The original negative staining carbon-film method depended on the availability of freshly prepared highly concentrated plant virus suspensions, as material stored at 4°C from 24hr to several weeks resulted in a considerable reduction in the areas of crystalline arrays. The addition of PEG, mol. wt. 6000, to stored plant virus suspensions containing cowpea chlorotic mottle virus, broad bean mottle virus, turnip rosette virus and southern bean mosaic virus has resulted in the formation of extensive continuous areas of crystalline arrays. 相似文献
15.
16.
Specimens of brain tissue obtained at autopsy from three patients suffering from progressive multifocal leukoencephalopathy (PML) were examined by electron microscopy. In specimens from all three cases particles similar to those of the papova virus group were present, confirming previous observations. By the negative staining method it was possible to define the morphological characteristics of the particles more precisely and it was shown that they are structurally similar to virions of the polyoma-SV40-K type. The need is emphasized for obtaining fresh unfixed diseased tissue from persons suffering from PML in order that the biological properties of the particles can be investigated. 相似文献
17.
Rapid Identification and Typing of Herpes Simplex Virus Types 1 and 2 by a Direct Immunofluorescence Technique 总被引:17,自引:5,他引:12
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An immunofluorescence (FA) technique has been developed which can identify herpes simplex virus (HSV) in clinical specimens and also type the virus directly as type 1 or type 2. This test, first applied to cervicovaginal specimens obtained from 80 mice genitally inoculated with HSV, indicated a sensitivity approaching 80% in comparison to standard viral isolation methods. A similar sensitivity was found when the test was applied to 185 clinical specimens with adequate cells for staining, which were obtained from a variety of sites of patients with suspect herpetic infection. In only 1 of 6 specimens positive by both FA and culture methods was the HSV type wrongly identified by the FA technique. There were also six specimens which were negative by culture methods but positive by the FA test, indicating a specificity of 91%. It is likely that these are not instances of false-positive tests but of other factors which may have resulted in negative viral isolations by culture methods. As more specific reagents become available, it is anticipated that the FA technique will have wider usage in diagnostic laboratories for the identification and typing of HSV types 1 and 2. 相似文献
18.
S Furui 《Microbiology and immunology》1986,30(10):1023-1035
A modified technique using protein A in the serum-in-agar (SIA) method for immune electron microscopy (IEM) was presented. Grids coated with staphylococcal protein A were floated on samples mounted on agar containing 2% antiserum and incubated at 37 C, for 60 min. After washing and staining, the grids were observed in an electron microscope. The effects of protein A on virus detection were evaluated using poliovirus and bovine rotavirus infected cell culture fluids. The results showed that the technique using protein A (PA-SIA) had at least 10-fold higher sensitivity for virus detection than the original SIA. The optimal concentration of protein A was 1 to 10 micrograms/ml for coating the grids to trap virus particles. The PA-SIA method was also compared with immunosorbent electron microscopy (ISEM). The former showed higher or at least the same sensitivity and some advantages in detecting antigen-antibody reaction than the latter method. These results indicate that our PA-SIA method may be superior to other IEM techniques presented previously for the detection and identification of viruses. 相似文献
19.
A new and rapid working method is presented for electronmicroscopic preparation of capsules from gram negative bacteria, e.g. Bordetella bronchiseptica and Pasteurella multocida. The advantage of the new technique is the availability of the results within 30 min after starting the preparation. The staining of the capsule by alcian blue has to be done as a first step together with glutaraldehyde fixation, before staining the bacterial cell with phosphotungstic acid. The new staining technic also reveals structural details of the capsule. The described procedure was found to be useful in controlling the development of the bacterial capsule depending on culture media for propagation and maintenance of the above mentioned bacteria. 相似文献
20.
D Zhao XM Yang QY Chen XS Zhang CJ Guo XY Che 《Journal of microbiological methods》2012,91(1):128-132
A modified acid-fast staining method was developed for rapid detection of Mycobacterium tuberculosis and its L forms, wherein carbol fuchsin and dioxogen were mixed into the sputum smear. With this method, the dyeing time is shortened and heating is not required. The sensitivity, specificity, positive predictive value, negative predictive value, positive rate, and diagnostic efficiency of the new method were compared to those obtained by PCR using 50 clinical samples. Further, 468 clinical samples were analyzed using the new method, the modified intensified Kinyoun (IK) acid-fast staining method, and the traditional Ziehl-Neelsen acid-fast staining method. Differences among the positive detection rates of the three methods were analyzed using Student's t-test, and no significant differences were found between the new method and the modified IK acid-fast staining method, while the rates of both these methods were higher than that of the traditional acid-fast staining method. Additionally, the dyeing time in the new method was markedly less than that in the modified IK acid-fast staining method (5min and 24h, respectively). 相似文献