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1.
Koyama S Nagahama T Ootsu N Takayama T Horii M Konishi S Miwa T Ishikawa Y Aizawa M 《Marine biotechnology (New York, N.Y.)》2005,7(4):272-278
We report successful larval hatching of deep-sea shrimp after decompression to atmospheric pressure. Three specimens of deep-sea shrimp were collected from an ocean depth of 1157 m at cold-seep sites off Hatsushima Island in Sagami Bay, Japan, using a pressure-stat aquarium system. Phylogenetic analysis of Alvinocaris sp. based on cytochrome c oxidase subunit gene sequences confirmed that these species were a member of the genus Alvinocaris. All 3 specimens survived to reach atmospheric pressure conditions after stepwise 63-day decompression. Two of the specimens contained eggs, which hatched after 10 and 16 days, respectively, of full decompression. Although no molting of the shrimp larvae was observed during 74 days of rearing under atmospheric pressure, the larvae developed conventional dark-adapted eyes after 15 days. 相似文献
2.
Effects of the piezo-tolerance of cultured deep-sea eel cells on survival rates, cell proliferation, and cytoskeletal structures 总被引:1,自引:0,他引:1
Koyama S Kobayashi H Inoue A Miwa T Aizawa M 《Extremophiles : life under extreme conditions》2005,9(6):449-460
We investigated the pressure tolerance of deep-sea eel (Simenchelys parasiticus; habitat depth, 366–2,630 m) cells, conger eel (Conger myriaster) cells, and mouse 3T3-L1 cells. Although there were no living mouse 3T3-L1 and conger eel cells after 130 MPa (0.1 MPa = 1 bar) hydrostatic pressurization for 20 min, all deep-sea eel cells remained alive after being subjected to pressures up to 150 MPa for 20 min. Pressurization at 40 MPa for 20 min induced disruption of actin and tubulin filaments with profound cell-shape changes in the mouse and conger eel cells. In the deep-sea eel cells, microtubules and some actin filaments were disrupted after being subjected to hydrostatic pressure of 100 MPa and greater for 20 min. Conger eel cells were sensitive to pressure and did not grow at 10 MPa. Mouse 3T3-L1 cells grew faster under pressure of 5 MPa than at atmospheric pressure and stopped growing at 18 MPa. Deep-sea eel cells were capable of growth in pressures up to 25 MPa and stopped growing at 30 MPa. Deep-sea eel cells required 4 h at 20 MPa to finish the M phase, which was approximately fourfold the time required under atmospheric conditions. 相似文献
3.
Tissue culture for the deep-sea clam Calyptogena soyoae (C. soyoae) has been examined. Mantle tissue was cultured in Dulbecco's modified Eagle medium that was prepared using artificial seawater
supplemented with fetal bovine serum (FBS) and the body fluid of C. soyoae. The mantle cells were viable in culture for at least 13 days at 4°C and atmospheric pressure on a polylysine-coated dish,
although no cells attached in the body fluid-free culture medium. It was found that mantle cells synthesized DNA and seemed
to proliferate under atmospheric conditions.
Received: June 1, 2000 / Accepted: October 4, 2000 相似文献
4.
Mohammad Hassan Qureshi Chiaki Kato Koki Horikoshi 《Extremophiles : life under extreme conditions》1998,2(2):93-99
We investigated for the first time the respiratory chain system of a deep-sea barophilic bacterium, Shewanella sp. strain DB-172F. A membrane-bound ccb-type quinol oxidase, from cells grown at 60 MPa pressure, was purified to an electrophoretically homogeneous state. The purified
enzyme complex consisted of four kinds of subunits with molecular masses of 98, 66, 18.5, and 15 kDa, and it contained 0.96
mol of protoheme and 1.95 mol of covalently bound heme c per mol of enzyme. Only protoheme in the enzyme reacted with CO and CN−, and the catalytic activity of the enzyme was 50% inhibited by 4 μM CN−. The isoelectric point of the native enzyme complex was determined to be 5.0. This enzyme was specifically induced only under
conditions of elevated hydrostatic pressure, and high levels were expressed in cells grown at 60 MPa. The membranes isolated
from cells grown at atmospheric pressure (0.1 MPa) exhibited high levels of both cytochrome c oxidase and N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPDH2)-oxidase activity. These results suggest the presence of two kinds of respiratory chains regulated
in response to pressure in the deep-sea bacterium DB-172F.
Received: November 25, 1997 / Accepted: December 25, 1997 相似文献
5.
Miroshnichenko ML Bonch-Osmolovskaya EA 《Extremophiles : life under extreme conditions》2006,10(2):85-96
The diversity of thermophilic prokaryotes inhabiting deep-sea hot vents was actively studied over the last two decades. The ever growing interest is reflected in the exponentially increasing number of novel thermophilic genera described. The goal of this paper is to survey the progress in this field made in the years 2000–2005. In this period, representatives of several new taxa of hyperthermophilic archaea were obtained from deep-sea environments. Two of these isolates had phenotypic features new for this group of organisms: the presence of an outer cell membrane (the genus Ignicoccus) and the ability to grow anaerobically with acetate and ferric iron (the genus Geoglobus). Also, our knowledge on the diversity of thermophilic bacteria from deep-sea thermal environments extended significantly. The new bacterial isolates represented diverse bacterial divisions: the phylum Aquificae, the subclass Epsilonproteobacteria, the order Thermotogales, the families Thermodesulfobacteriaceae, Deferribacteraceae, and Thermaceae, and a novel bacterial phylum represented by the genus Caldithrix. Most of these isolates are obligate or facultative lithotrophs, oxidizing molecular hydrogen in the course of different types of anaerobic respiration or microaerobic growth. The existence and significant ecological role of some of new bacterial thermophilic isolates was initially established by molecular methods. 相似文献
6.
Sotaro Fujii Misa Masanari-Fujii Shinya Kobayashi Chiaki Kato Masayoshi Nishiyama Yoshie Harada 《Bioscience, biotechnology, and biochemistry》2018,82(5):792-799
Two cytochromes c5 (SBcytc and SVcytc) have been derived from Shewanella living in the deep-sea, which is a high pressure environment, so it could be that these proteins are more stable at high pressure than at atmospheric pressure, 0.1 MPa. This study, however, revealed that SBcytc and SVcytc were more stable at 0.1 MPa than at higher pressure. In addition, at 0.1–150 MPa, the stability of SBcytc and SVcytc was higher than that of homologues from atmospheric-pressure Shewanella, which was due to hydrogen bond formation with the heme in the former two proteins. This study further revealed that cytochrome c551 (PMcytc) of deep-sea Pseudomonas was more stable than a homologue of atmospheric-pressure Pseudomonas aeruginosa, and that specific hydrogen bond formation with the heme also occurred in the former. Although SBcytc and SVcytc, and PMcytc are phylogenetically very distant, these deep-sea cytochromes c are commonly stabilized through hydrogen bond formation. 相似文献
7.
Isolation of extremophiles with the detection and retrieval of<Emphasis Type="Italic"> Shewanella</Emphasis> strains in deep-sea sediments from the west Pacific 总被引:10,自引:0,他引:10
Tests to detect the presence of piezophilic Shewanella strains in the deep-sea sediments of the west, mid- and east Pacific at different depths were done by amplification of previously identified pressure-regulated operons (ORF1,2 and ORF3). The operon fragments were detected in all the deep-sea sediment samples, indicating the broad presence of piezophilic deep-sea Shewanella species or related species in the deep-sea sediments across the Pacific. Extremophiles were isolated from the deep-sea sediment of the west Pacific under atmospheric pressure. Two psychrophilic/psychrotrophic strains, WP2 and WP3, were assigned to the Shewanella genus as determined by their 16S rDNA sequences. WP2 and WP3 were both capable of amplifying pressure-regulated operons; the sequences of the pressure-regulated operons of WP2 and WP3 share high identity between each other, but have more differences from those of S. benthica and S. violacea. The major fatty acids of WP2 and WP3 are 3OH-i-13:0, 14:0, i-15:0, 16:0, 16:1, 18:1, and 20:5. Combined phenotypic analysis, 16S rDNA sequences, and DNA–DNA hybridization results suggest that WP2 and WP3 are two new deep-sea Shewanella species.Communicated by K. Horikoshi 相似文献
8.
Nitrile metabolising actinomycetes previously recovered from deep-sea sediments and terrestrial soils were investigated for
their nitrile transforming properties. Metabolic profiling and activity assays confirmed that all strains catalysed the hydrolysis
of nitriles by a nitrile hydratase/amidase system. Acetonitrile and benzonitrile, when used as growth substrates for enzyme
induction experiments, had a significant influence on the biotransformation activities towards various nitriles and amides.
The specific activities of selected deep-sea and terrestrial acetonitrile-grown bacteria against a suite of nitriles and amides
were higher than those of the only other reported marine nitrile-hydrolysing R. erythropolis, isolated from a shallow sediment. The increase of nitrile chain length appeared to have negative influence on the nitrile
hydratase activity of acetonitrile-grown bacteria, but the same was not true for benzonitrile-grown bacteria. The nitrile
hydratases and amidases were constitutive in 10 of the 16 deep-sea and terrestrial actinomycetes studied, and one strain showed
an inducible hydratase and a constitutive amidase. Most of the deep-sea strains had constitutive activities and showed some
of the highest activities and broadest substrate specificities of organisms included in this study.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
9.
Yoshizumi Nakagawa Yoshimi Suzuki 《Journal of experimental marine biology and ecology》2008,362(1):32-37
Incubation of unaltered deep-sea water and grazing experiment of nano- and micro- protozooplankton during incubation of deep-sea water were carried out to quantitatively characterize the planktonic structures of lower-trophic organisms and clarify the trophic pathways and controlling mechanisms involved. Phytoplankton biomass increased to 637 mg as carbon weight in a 500-l tank on Day 7 and was dominant in the planktonic structure of lower-trophic organisms. Nitrates in the incubation water was depleted after Day 7 and phytoplankton biomass decreased rapidly. On the other hand, bacteria, heterotrophic nano-flagellates and ciliates increased toward the end of incubation and were dominant in the later days of incubation. In grazing experiments on microbial organisms, bacterivory is more important for the carbon pathway in microbial food webs than herbivory when phytoplankton biomass is less than that of bacteria (low P/B conditions), while herbivory is more important than bacterivory when phytoplankton biomass is more than that of bacteria (high P/B conditions). Deep-sea water exhibited high phytoplankton productivity due to inherent high nutrients values. After depletion of nutrients, phytoplankton decreased (due also to enhanced nano- and micro-zooplankton grazing) and microbial organisms dominated. Thus, nutrients in the incubation water control the planktonic structure of lower-trophic organisms. 相似文献
10.
We used DNA transfection and protein introduction techniques to investigate the pressure tolerance of cytoskeletal structures
in pectoral fin cells derived from the deep-sea fish Simenchelys parasiticus (habitat depth, 366–2,630 m). The deep-sea fish cells have G418 resistance. The cell number increased until day 6 of cultivation
and all cells had died by day 35 when cultured in 35-mm Petri dishes in medium containing G418. Enhanced yellow fluorescent
protein-tagged human β-actin (EYFP-actin) was stably expressed by 1 in 100,000 deep-sea fish cells. Because almost none of
the EYFP-actin was incorporated into actin filaments of the cells, we replaced the relatively large EYFP tag with a chemical
fluorescent compound and succeeded in incorporating fluorescently labeled rabbit actins into the deep-sea fish actin filaments.
Most of the filament structure in the cells with rabbit actin inserted underwent depolymerization when subjected to pressure
of 100 MPa for 20 min, in contrast to control cells. There were no differences in the tubulin filament structure between control
cells and deep-sea fish cells with fluorescein-labeled bovine tubulin inserted after the application of pressure ranging from
40 to 100 MPa for 20 min. 相似文献
11.
Kaoru Nakasone Akihiko Ikegami C. Kato R. Usami Koki Horikoshi 《Extremophiles : life under extreme conditions》1998,2(3):149-154
A pressure-regulated operon has been cloned and sequenced from deep-sea barophilic Shewanella strains. To understand pressure-regulated mechanisms of gene expression, a regulatory element upstream of the pressure-regulated
operon from Shewanella sp. strain DSS12 was studied. Regions A and B were classified by sequence analysis. A unique octamer motif, AAGGTAAG, was
found to be repeated in tandem 13 times in region B. An electrophoretic mobility shift assay demonstrated that a σ54-like factor recognizes region A and other unknown factors recognize region B. Different shift patterns of the protein–DNA
complexes were observed when extracts of cells cultured at 0.1 MPa or 50 MPa were incubated with a DNA probe specific for
region B. These results indicate that the deep-sea strain DSS12 expresses different DNA-binding factors under different pressure
conditions.
Received: January 22, 1998 / Accepted: February 16, 1998 相似文献
12.
Florence Pradillon Françoise Gaill 《Reviews in Environmental Science and Biotechnology》2007,6(1-3):181-195
All biological processes of life on Earth experience varying degrees of pressure. Aquatic organisms living in the deep-sea, as well as chondrocytic cells of articular cartilage are exposed to hydrostatic pressures that rise up to several hundred times that of atmospheric pressure. In the case of marine larvae that disperse through the oceanic water column, pressure changes might be responsible for stress conditions during development, limiting colonisation capabilities. In a number of biological systems, life strategies may be significantly influenced by pressure. In this review, we will focus on the consequences of pressure changes on various biological processes, and more specifically on animals living in the deep-sea. Revisiting general principles of pressure effects on biological systems, we present recent data illustrating the diversity of effects pressure may have at different levels in biological systems, with particular attention to effects on gene expression. After a review of the main pressure equipments available today for studying species living naturally at high pressure, we summarise what is known concerning pressure impact during animal development. 相似文献
13.
Rupture of the Cell Envelope by Decompression of the Deep-Sea Methanogen Methanococcus jannaschii
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The effect of decompression on the structure of Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen, was studied in a novel high-pressure, high-temperature bioreactor. The cell envelope of M. jannaschii appeared to rupture upon rapid decompression (ca. 1 s) from 260 atm of hyperbaric pressure. When decompression from 260 atm was performed over 5 min, the proportion of ruptured cells decreased significantly. In contrast to the effect produced by decompression from hyperbaric pressure, decompression from a hydrostatic pressure of 260 atm did not induce cell lysis. 相似文献
14.
Grote R Li L Tamaoka J Kato C Horikoshi K Antranikian G 《Extremophiles : life under extreme conditions》1999,3(1):55-62
A novel coccoid-shaped, hyperthermophilic, anaerobic archaeon, strain RG-20, was isolated from a deep-sea hydrothermal vent
fluid sample taken at 1394-m depth at the Mid-Okinawa Trough (27°32.7′N, 126°58.5′E). Cells of this isolate occur singly or
in pairs and are about 0.8 to 2 μm in diameter. Growth was observed at temperatures between 50° and 93°C, with an optimum
at 85°C. The pH range for growth is 5.0–9.0, with an optimum around 7.0. Strain RG-20 requires 1%–4% of NaCl for growth, and
cell lysis occurs at concentrations below 1%. The newly isolated strain grows preferentially in the presence of elemental
sulfur on proteinaceous substrates such as yeast extract, peptone, or tryptone, and no growth was observed on carbohydrates,
carboxylic acids, alcohols, or lipids. This microorganism is resistant to streptomycin, chloramphenicol, ampicillin, and kanamycin
at concentrations up to 150 μg/ml, but is susceptible to rifampicin. Analysis of the hydrolyzed core lipids by thin-layer
chromatography (TLC) revealed the presence of archaeol and caldarchaeol. The mol% G+C content of the DNA is 55.8. Partial
sequencing of the 16S rDNA indicates that strain RG-20 belongs to the genus Thermococcus. Considering these data and on the basis of the results from DNA-DNA hybridization studies, we propose that this strain should
be classified as a new species named Thermococcus siculi (si′cu.li. L. gen. n. siculi, of the deep-sea [siculum, deep-sea in literature of Ovid], referring to the location of the sample site, a deep-sea hydrothermal vent). The type strain
is isolate RG-20 (DSM No. 12349).
Received: May 11, 1998 / Accepted: July 24, 1998 相似文献
15.
16.
Antibacterial and antilarval activity of deep-sea bacteria from sediments of the West Pacific Ocean 总被引:1,自引:0,他引:1
Abstract Deep-sea microorganisms are a new source of bioactive compounds. In this study, crude ethyl acetate extracts of 176 strains of deep-sea bacteria, isolated from sediments of the West Pacific Ocean, were screened for their antibacterial activity against four test bacterial strains isolated from marine biofilms. Of these, 28 deep-sea bacterial strains exhibited antibacterial activity against one or more of the bacteria tested. Active deep-sea bacterial strains belonged mainly to the genera of Pseudomonas, Psychrobacter and Halomonas. Additionally, antilarval activity of 56 deep-sea bacterial strains was screened using Balanus amphitrite larvae. Seven bacterial strains produced metabolites that had strong inhibitive effects on larval settlement. None of these metabolites showed significant toxicity. The crude extract of one deep-sea Streptomyces strain could completely inhibit larval settlement at a concentration of 25 μg ml?1. 相似文献
17.
Stefania E. Kapsetaki Stuart A. West 《Evolution; international journal of organic evolution》2019,73(6):1296-1308
The first step in the evolution of complex multicellular organisms involves single cells forming a cooperative group. Consequently, to understand multicellularity, we need to understand the costs and benefits associated with multicellular group formation. We found that in the facultatively multicellular algae Chlorella sorokiniana: (1) the presence of the flagellate Ochromonas danica or the crustacean Daphnia magna leads to the formation of multicellular groups; (2) the formation of multicellular groups reduces predation by O. danica, but not by the larger predator D. magna; (3) under conditions of relatively low light intensity, where competition for light is greater, multicellular groups grow slower than single cells; (4) in the absence of live predators, the proportion of cells in multicellular groups decreases at a rate that does not vary with light intensity. These results can explain why, in cases such as this algae species, multicellular group formation is facultative, in response to the presence of predators. 相似文献
18.
Marteinsson VT Reysenbach AL Birrien JL Prieur D 《Extremophiles : life under extreme conditions》1999,3(4):277-282
The whole-cell protein inventory of the deep-sea barophilic hyperthermophile Thermococcus barophilus was examined by one-dimensional SDS gradient gel electrophoresis when grown under different pressure conditions at 85°C (T
opt). One protein (P60) with a molecular mass of approximately 60 kDa was prominent at low pressures (0.3 MPa hydrostatic pressure
and 0.1 MPa atmospheric pressure) but not at deep-sea pressures (10, 30, and 40 MPa). About 17 amino acids were sequenced
from the N-terminal end of the protein. Sequence homology analysis in the GenBank database showed that P60 most closely resembled
heat-shock proteins in some sulfur-metabolizing Archaea. A high degree of amino acid identity (81%–93%) to thermosome subunits
in Thermococcales strains was found. Another protein (P35) with molecular mass of approximately 35.5 kDa was induced at 40 MPa hydrostatic
pressure but not under low-pressure conditions. No amino acid sequence homology was found for this protein when the 40 amino
acids from the N-terminal end were compared with homologous regions of proteins from databases. A PTk diagram was generated for T. barophilus. The results suggest that P
habitat is about 35 MPa, which corresponds to the in situ pressure where the strain was obtained.
Received: May 14, 1999 / Accepted: July 30, 1999 相似文献
19.
A key step in the evolution of multicellular organisms is the formation of cooperative multicellular groups. It has been suggested that predation pressure may promote multicellular group formation in some algae and bacteria, with cells forming groups to lower their chance of being eaten. We use the green alga Chlorella vulgaris and the protist Tetrahymena thermophila to test whether predation pressure can initiate the formation of colonies. We found that: (1) either predators or just predator exoproducts promote colony formation; (2) higher predator densities cause more colonies to form; and (3) colony formation in this system is facultative, with populations returning to being unicellular when the predation pressure is removed. These results provide empirical support for the hypothesis that predation pressure promotes multicellular group formation. The speed of the reversion of populations to unicellularity suggests that this response is due to phenotypic plasticity and not evolutionary change. 相似文献
20.
The effect of decompression on the structure of Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen, was studied in a novel high-pressure, high-temperature bioreactor. The cell envelope of M. jannaschii appeared to rupture upon rapid decompression (ca. 1 s) from 260 atm of hyperbaric pressure. When decompression from 260 atm was performed over 5 min, the proportion of ruptured cells decreased significantly. In contrast to the effect produced by decompression from hyperbaric pressure, decompression from a hydrostatic pressure of 260 atm did not induce cell lysis. 相似文献