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《现代生物医学进展》2007,7(10):I0001-I0002
科学网报道:很多科学家认为,维C等抗氧化剂能够阻止肿瘤生长的原因在于,它们能够夺取不稳定的氧自由基分子以避免它们对人体DNA造成伤害。然而,美国约翰霍普金斯大学研究人员的最新一项研究表明,抗氧化剂的真正作用有可能在于减弱肿瘤在缺氧条件下的生存能力。相关论文9月11日发表于《癌细胞》(Cancer Cell)上。[第一段] 相似文献
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循环肿瘤细胞(circulating tumor cell,CTC)是随血液循环一起转运的实体肿瘤细胞,与实体肿瘤的发展、转移、复发和预后等关系密切。然而,CTC数量的稀少使有效检测CTC具有较大的挑战性。微小RNA(microRNA,miRNA)作为一类新发现的基因表达调控分子,在肿瘤的发生、发展、转归等过程中起着重要的作用。CTC关联性miRNA的研究为CTC的检测和肿瘤的诊治开创了新思路。该文介绍了CTC的临床意义和主要分析方法,在CTC关联性miRNA与肿瘤诊断、治疗和预后等方面总结了这类新型肿瘤细胞标志物的研究进展。 相似文献
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肌肉生长抑制素(myostatin,MSTN)属于转化生长因子-β(transforming growth factor-β,TGF-β)超家族,主要功能为负向调节骨骼肌的生长.肌肉生长抑制素基因敲除小鼠肌肉出现显著增加,而将干涉该基因的短发夹RNA注射并电击转化入大鼠胫前肌则引起肌肉重量、肌纤维以及MHCⅡ表达的增加.通过与小鼠肌肉生长抑制素基因表达载体共转染HEK293细胞,筛选到两条能够高效抑制小鼠肌肉生长抑制素基因表达的小干涉RNA.构建了这两条小RNA的表达载体Mst-shRNA1和Mst-shRNA2,用其分别转染小鼠C2C12成肌细胞,并通过G418药物筛选和流式细胞仪富集整合了短发夹RNA表达载体的阳性细胞.通过采用Real-time PCR和Western blot分析,检测到在分别整合了Mst-shRNA1和Mst-shRNA2的C2C12细胞中,内源性肌肉生长抑制素基因的mRNA水平分别下降了10.2%和35.5%,蛋白质表达则分别下降了29.3%和64.7%.同时,在这两组中MyoD的表达上升了24.4%和40.4%,证明通过RNA干涉实现的肌肉生长抑制素基因的抑制导致了下游MyoD基... 相似文献
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Ting Zhang ;Jingyao Wang ;Xiaofeng Zhai ;Hongjie ;Changying Li ;Jiwu Chang 《Acta biochimica et biophysica Sinica》2014,(12):1072-1079
MicroRNAs (miRNAs) are a class of non-coding small RNAs that act as negative regulators of gene expression by binding to the 31-untranslated region (3'UTR) of target mRNAs. In order to investigate the physiological role of miR-124 in bladder cancer, target genes of miR-124 were predicted by the TargetScan software, and cyclin-depend- ent kinase (CDK4), which has been implicated as a regula- tor of cell cycle, was chosen for further study. MiR-124 could significantly repress CDK4 expression by targeting its binding site in the 31UTR of CDK4 in vitro. In both bladder cancer cell lines and tissues, the expression of miR- 124 was significantly down-regulated, while CDK4 expres- sion was up-regulated. Ectopic expression of miR-124 in transplanted HTl197 cells resulted in the retardation of tumor growth in mouse tumor xenografts. And the expres- sion of miR-124 and CDK4 showed an obvious inverse cor- relation in these xenograft tissues, which was also observed in human bladder cancer tissue samples. Taken together, our results strongly suggest that miR-124 can arrest cell cycle and restrain the growth of bladder cancer by targeting CDK4 directly. 相似文献
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Jun-Ming Liao Bo Cao XiangZhou Hua Lu 《分子细胞生物学报》2014,(3):206-213
The tumor suppressor p53 pathway, whose alterations are highly associated with all types of human cancers, plays an essential role in preventingtumor development and progression mostly through its downstream target genes. Over the last decade, a growing list of p53 microRNA (miRNA) targets has been identified as additional downstream players of this pathway. Further studies ofthese miRNAs have revealed their more complicated regulations and functions in executing and/or regulating p53 activity. Here, we review the p53 miRNA targets identified thus far, and discuss how they fine-tune p53 stress responses, mediate the crosstalk between p53 and other signaling pathways, and expand the role of p53 in other human diseases in addition to cancers. 相似文献
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目的:构建针对猪肌肉生长抑制素mRNA的RNA干涉载体,并体内验证其有效性。方法:分别体外转染化学合成4个19 bp的siRNA片段获取针对肌肉生长抑制素的序列,构建RNA干涉载体,将上述载体注射到猪股四头肌中,RT-PCR检测证明其表达有效性及生肌调节因子mRNA水平的变化。结果:获得了针对肌肉生长抑制素的2个小干涉RNA序列,构建了2个重组载体,股四头肌注射混合后的等量重组载体,RT-PCR显示注射重组RNA干涉载体可显著降低肌肉生长抑制素的mRNA水平(约降低了40%),生肌调节因子mRNA水平显著上调(分别约为对照组的2.1~3.9倍)。结论:该试验为在转基因动物的肌肉中表达外源基因奠定了基础。 相似文献
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