共查询到19条相似文献,搜索用时 46 毫秒
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长链非编码RNA(long non-coding RNA,lncRNA),是一种长度大于200个核苷酸的调控性非编码RNA,能在转录水平、转录后水平及表观遗传水平等多个层面影响基因的表达。脂肪生成是一个复杂而有序的过程。大量研究表明,lncRNA在脂肪生成过程中扮演着重要角色,它可以影响脂质代谢及成脂分化等多种生物过程,从而间接影响肉品质。这对于提高畜禽肉品质、避免养殖业饲料过多转化成脂肪所导致的浪费以及对预防和治疗与脂肪代谢相关的疾病都具有重要意义。对lncRNA的基本特征、在动物脂肪沉积中的作用进展进行了综述,以期为培育优质畜禽,预防和治疗与脂肪代谢相关的疾病提供理论依据。 相似文献
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为研究无量山乌骨鸡(Gallus gallus)肝组织脂代谢相关miRNA (microRNA)在不同发育阶段的表达特征,本研究采集出壳当日(D1)和168日龄(D168)母鸡肝组织样品作为试验材料,利用DNBSEQ平台进行测序,采用DEGseq筛选差异表达的miRNA及其靶基因,随机选取9个差异表达miRNA进行RT-qPCR验证,KEGG通路分类筛选出脂代谢相关miRNA并进行聚类分析,预测脂代谢相关miRNA靶基因并进行GO和KEGG通路功能富集,构建脂代谢相关miRNA和靶基因关联网络。分析结果表明,筛选出106个差异表达miRNAs,包括54个上调miRNA和52个下调miRNA;聚类得到41个脂代谢相关的miRNAs;预测到38个靶基因,对靶基因的功能注释确定主要富集于甘油磷脂代谢、脂肪酸代谢和鞘脂代谢等脂质代谢相关通路,novel-gga-miR2311-5p-DGKZ、 novel-gga-miR2047-3p-ACACA、 novel-gga-miR866-3p-DGKH是脂代谢相关候选miRNA-mRNA关系对。研究提示无量山乌骨鸡肝组织miRNA在不同发育阶段的表... 相似文献
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鼻咽癌(nasopharyngeal carcinoma,NPC)是一种多基因遗传性疾病,好发生于我国华南、东南亚及部分非洲地区。近年来随着分子生物学及其技术的迅速发展,人们对鼻咽癌发生、发展及其生物学行为的研究已进入基因水平。microRNA(miRNA)是一类广泛存在于动植物体内的非编码小RNA,主要参与基因转录后水平调控。随着对miRNA研究的深入,发现肿瘤的细胞分化障碍、增殖失控、细胞永生化与miRNA密切相关。人类肿瘤组织与正常细胞组织间的miRNA表达水平和类型存在明显差异,提示miRNA可能是一类新的参与肿瘤发生的重要分子。本文就鼻咽癌与miRNA相关的研究进展作一综述。 相似文献
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鼻咽癌(nasopharyngeal carcinoma, NPC)是一种多基因遗传性疾病,好发生于我国华南、东南亚及部分非洲地区。近年来随着分子生物学及其技术的迅速发展,人们对鼻咽癌发生、发展及其生物学行为的研究已进入基因水平。microRNA(miRNA)是一类广泛存在于动植物体内的非编码小RNA,主要参与基因转录后水平调控。随着对miRNA研究的深入,发现肿瘤的细胞分化障碍、增殖失控、细胞永生化与miRNA密切相关。人类肿瘤组织与正常细胞组织间的miRNA表达水平和类型存在明显差异,提示miRNA可能是一类新的参与肿瘤发生的重要分子。本文就鼻咽癌与miRNA相关的研究进展作一综述。 相似文献
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姜黄素(curcumin)是近年来发现的一种具有多种生物学活性的中药单体,研究表明姜黄素能够杀伤多种肿瘤细胞,显示出良好的防癌和抗癌作用,美国国立卫生(nationalinstituteofhealth,N/H)已将姜黄素列为第三代化学预防药,相关的临床试验已经展开。微小RNA(micmRNA,miRNA)是细胞内高度保守的非编码RNA,参与了包括肿瘤在内的多个病理生理过程。最新研究表明姜黄素通过调控miRNA机制发挥抗癌作用,我们的研究亦证实姜黄素影响肺癌细胞特异性miRNA表达引起肿瘤细胞凋亡和逆转耐药。总结最新研究进展,本文就姜黄素在不同系统肿瘤细胞中通过调控特异性miRNA发挥抗癌作用的机制作一综述。 相似文献
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脂代谢是心肌能量代谢网络的重要环节,其稳态的调控是心肌适应不同病理、生理状态的关键.运动是心肌脂代谢稳态的重要调节手段,大量研究发现miRNAs参与其中.该文将综述细胞内与脂代谢相关miRNAs的作用机制,阐释循环miRNAs对一次性运动的反应和对长期规律性运动的适应,以及心肌miRNAs表达对不同运动方式的适应,并探... 相似文献
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Bonnet M Cassar-Malek I Chilliard Y Picard B 《Animal : an international journal of animal bioscience》2010,4(7):1093-1109
The lean-to-fat ratio, that is, the relative masses of muscle and adipose tissue, is a criterion for the yield and quality of bovine carcasses and meat. This review describes the interactions between muscle and adipose tissue (AT) that may regulate the dynamic balance between the number and size of muscle v. adipose cells. Muscle and adipose tissue in cattle grow by an increase in the number of cells (hyperplasia), mainly during foetal life. The total number of muscle fibres is set by the end of the second trimester of gestation. By contrast, the number of adipocytes is never set. Number of adipocytes increases mainly before birth until 1 year of age, depending on the anatomical location of the adipose tissue. Hyperplasia concerns brown pre-adipocytes during foetal life and white pre-adipocytes from a few weeks after birth. A decrease in the number of secondary myofibres and an increase in adiposity in lambs born from mothers severely underfed during early pregnancy suggest a balance in the commitment of a common progenitor into the myogenic or adipogenic lineages, or a reciprocal regulation of the commitment of two distinct progenitors. The developmental origin of white adipocytes is a subject of debate. Molecular and histological data suggested a possible transdifferentiation of brown into white adipocytes, but this hypothesis has now been challenged by the characterization of distinct precursor cells for brown and white adipocytes in mice. Increased nutrient storage in fully differentiated muscle fibres and adipocytes, resulting in cell enlargement (hypertrophy), is thought to be the main mechanism, whereby muscle and fat masses increase in growing cattle. Competition or prioritization between adipose and muscle cells for the uptake and metabolism of nutrients is suggested, besides the successive waves of growth of muscle v. adipose tissue, by the inhibited or delayed adipose tissue growth in bovine genotypes exhibiting strong muscular development. This competition or prioritization occurs through cellular signalling pathways and the secretion of proteins by adipose tissue (adipokines) and muscle (myokines), putatively regulating their hypertrophy in a reciprocal manner. Further work on the mechanisms underlying cross-talk between brown or white adipocytes and muscle fibres will help to achieve better understanding as a prerequisite to improving the control of body growth and composition in cattle. 相似文献
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Yusuke Fujimoto Osamu Hashimoto Daichi Shindo Makoto Sugiyama Shozo Tomonaga Masaru Murakami Tohru Matsui Masayuki Funaba 《Journal of cellular biochemistry》2019,120(1):821-835
Brown and beige adipocytes dissipate energy as heat. Thus, the activation of brown adipocytes and the emergence of beige adipocytes in white adipose tissue (WAT) are suggested to be useful for preventing and treating obesity. Although β3-adrenergic receptor activation is known to stimulate lipolysis and activation of brown and beige adipocytes, fat depot–dependent changes in metabolite concentrations are not fully elucidated. The current study examined the effect of treatment with CL-316,243, a β3-adrenergic receptor agonist, on the relative abundance of metabolites in interscapular brown adipose tissue (iBAT), inguinal WAT (ingWAT), and epididymal WAT (epiWAT). Intraperitoneal injection of CL-316,243 (1 mg/kg) for 3 consecutive days increased the relative abundance of several glycolysis-related metabolites in all examined fat depots. The cellular concentrations of metabolites involved in the citric acid cycle and of free amino acids were also increased in epiWAT by CL-316,243. CL-316,243 increased the expression levels of several enzymes and transporters related to glucose metabolism and amino acid catabolism in ingWAT and iBAT but not in epiWAT. CL-316,243 also induced the emergence of more beige adipocytes in ingWAT than in epiWAT. Furthermore, adipocytes surrounded by macrophages were detected in the epiWAT of mice given CL-316,243. The current study reveals the fat depot–dependent modulation of cellular metabolites in CL-316,243-treated mice, presumably resulting from differential regulation of cell metabolism in different cell populations. 相似文献
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Mito Toyoda Yoshinori Matsubara Konghua Lin Keizou Sugimachi Masutaka Furue 《Cell biochemistry and function》2009,27(7):440-447
Different fat depots contribute differently to disease and function. These differences may be due to the regional variation in cell types and inherent properties of fat cell progenitors. To address the differences of cell types in the adipose tissue from different depots, the phenotypes of freshly isolated adipose tissue‐derived cells (ATDCs) from subcutaneous (SC) and omental (OM) adipose tissues were compared using flow cytometry. Our results showed that CD31?CD34+CD45?CD90‐CD105?CD146+ population, containing vascular smooth muscle cells and pericytes, was specifically defined in the SC adipose tissue while no such population was observed in OM adipose tissue. On the other hand, CD31?CD34+CD45?CD90?CD105?CD146? population, which is an undefined cell population, were found solely in OM adipose tissue. Overall, the SC adipose tissue contained more ATDCs than OM adipose tissue, while OM adipose tissue contained more blood‐derived cells. Regarding to the inherent properties of fat cell progenitors from the two depots, adipose‐derived stem cells (ADSCs) from SC had higher capacity to differentiate into both adipogenic and osteogenic lineages than those from OM, regardless of that the proliferation rates of ADSCs from both depots were similar. The higher differentiation capacity of ADSCs from SC adipose tissue suggests that SC tissue is more suitable cell source for regenerative medicine than OM adipose tissue. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
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Jack R. T. Darby Alexandra Sorvina Christie A. Bader Mitchell C. Lock Jia Yin Soo Stacey L. Holman Mike Seed Tim Kuchel Douglas A. Brooks Sally E. Plush Janna L. Morrison 《Journal of biophotonics》2020,13(3)
The primary metabolic pathway required to produce ATP differs as a result of tissue type, developmental stage and substrate availability. We utilized molecular and histological techniques to define the metabolic status in foetal and adult, adipose and skeletal muscle tissues. Redox ratios of these tissues were also determined optically by two‐photon microscopy. Adult perirenal adipose tissue had a higher optical redox ratio than fetal perirenal adipose tissue, which aligned with glycolysis being used for ATP production; whereas adult skeletal muscle had a lower optical redox ratio than fetal skeletal muscle, which aligned with oxygen demanding oxidative phosphorylation activity being utilized for ATP production. We have compared traditional molecular and microscopy techniques of metabolic tissue characterization with optical redox ratios to provide a more comprehensive report on the dynamics of tissue metabolism. 相似文献
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Gruffat D Gobert M Durand D Bauchart D 《Animal : an international journal of animal bioscience》2011,5(7):1090-1098
Feeding strategies based on the addition of plant lipids rich in polyunsaturated fatty acids (PUFAs) in diets of bovines during the finishing period are common to enhance the nutritional value of meat. However, following rumen biohydrogenations, these FAs could still be metabolised in various tissues/organs involved in the FA metabolism such as the liver and adipose tissues (ATs), thus affecting their subsequent deposition in muscles. In this context, the objective of this study was to characterise the various metabolic pathways of linoleic acid (LA) and α-linolenic acid (ALA) in the liver and ATs (subcutaneous (SC) and inter-muscular (IM)) of Normande cull cows fed a diet supplemented (LR) or not (C) with extruded linseeds and rapeseeds, using the ex vivo incubated tissue slice method. Hepatic uptake of both FAs was higher with the LR than with the C diet (P = 0.02). For the two diets, ALA uptake was higher than that of LA (+46%, P = 0.04). ALA was much more degraded by β-oxidation (>50% of ALA present in cells) than LA (~27%) with both diets (P = 0.015). Whatever the diet, ALA was not converted into longer and/or more unsaturated FA, whereas about 14% of LA was converted into 20:4n-6. The intensity of the esterification pathway was higher (+70%, P = 0.004) with the LR than with the C diet, for both FAs. Hepatic secretion of ALA as part of the very-low-density lipoprotein particles was lower than that of LA (-58% and -23% for C and LR diets respectively, P = 0.02). In SC and IM ATs, dietary lipid supplementation did not alter metabolic pathways of LA and ALA. They were efficiently taken up by ATs (>68% of FA present in the medium), with uptake being higher for IM than for SC AT (+12%, P = 0.01). Moreover, LA uptake by ATs was higher than ALA uptake (+10.7%, P = 0.027). Both FAs were mainly esterified (>97% of FA present in adipocytes) into neutral lipids (>85% of esterified FA). Around 9.5% of LA was converted into 20:4n-6, whereas only around 1.3% of ALA was converted into 20:5n-3. We concluded that, in our experimental conditions, liver was highly active in ALA catabolism limiting its subsequent deposition in muscles. However, bovine liver and ATs were inefficient at converting ALA into long-chain n-3 PUFA, but actively converted LA into 20:4n-6. 相似文献