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1.
Evoked electrical discharges in the spinal cord roots and dorsal surface ipsilateral to the previously severed sciatic nerve (as well as on the contralateral side) were investigated in rats one, three, seven, and 14 days after tractotomy. Monosynaptic reflex discharges in the ventral roots were found to return to 20–40% of the level of this parameter as measured on the contralateral side within seven and 14 days after tractotomy. Mean amplitude of antidromic dorsal root discharges, afferent peak, and the N1 component of potential(s) at the dorsal surface ipsilateral to the severed nerve barely altered, remaining significantly lower than on the contralateral side. Mechanisms are suggested for the increase in monosynaptic reflex ventral root discharges ipsilateral to the severed nerve following tractotomy — thought to be largely due to raised sensitivity to transmitter at the motoneuronal membrane resulting from degeneration of synapses of descending pathways.Medical Institute of the Ukrainian Ministry of Health, Dnepropetrovsk. Translated from Neirofiziologiya, Vol. 21, No. 3, pp. 366–371, May–June, 1989.  相似文献   

2.
Experiments on superfused isolated spinal cord preparations from rats aged 8–13 days showed that noradrenal in and serotonin have only a weak effect on monosynaptic reflex discharges but a substantial effect on polysynaptic motoneuronal discharges: noradrenalin potentiates whereas serotonin inhibits them. Both amines inhibit dorsal root potentials evoked by stimulation of high-threshold afferents. Potentiation of polysynaptic motoneuronal discharges induced by noradrenalin is connected with hyperpolarization of high-threshold afferents due to inhibition of the function of neurons in the substantia gelatinosa, and with increased excitability of interneurons participating in the generation of motoneuronal discharges. Serotonin inhibits polysynaptic motoneuronal discharges through its direct depolarizing effect on terminals of high-threshold afferents and depression of interneuron activity responsible for these discharges. Adrenergic and serotonin receptors, mediating these effects of noradrenalin and serotonin, were subjected to pharmacologic analysis.A. M. Gor'kii Donetsk Medical Institute. Translated from Neirofiziologiya, Vol. 14, No. 3, pp. 241–247, May–June, 1982.  相似文献   

3.
Experiments on anesthetized spinal cats showed that ammonium acetate, injected intravenously (2–4 mmoles/kg) inhibits the depolarization of the central endings of primary afferent fibers activated by stimulation of afferent nerves. Inhibition of primary afferent depolarization is transient in character and develops parallel with depression of postsynaptic inhibition of monosynaptic reflexes. The depression produced by the action of ammonium was not due to blocking of negative postsynaptic potentials of the dorsal surface of the spinal cord or blocking of reflex electrical discharges in the ventral spinal roots. It is suggested that depression of primary afferent depolarization is due to a decrease in the emf for synaptic ion currents producing depolarization.Allergologic Research Laboratory, Academy of Medical Sciences of the USSR, Moscow. Translated from Neirofiziologiya, Vol. 9, No. 1, pp. 52–60, January–February, 1977.  相似文献   

4.
Effects of the glucocorticoid hormone dexamethasone on the reflex discharges in the lumbar ventral roots and background activity (BA) of single neurons in the dorsal laminae of spinal grey were studied in rats after transection of the sciatic nerve. Administration of the hormone during early post-traumatic period (up to seven days) evoked no significant changes in the amplitude of increased (due to the postdenervation hyperreflexia) monosynaptic discharges on the side of nerve transection. At the same time, the monosynaptic discharges grew by 150–170% on the intact side. During later post-transection periods (up to 35 days), when ventral root reflex discharges were suppressed, dexamethasone facilitated reflex transmission via the polysynaptic segmental pathways on both the operated and intact sides. Nonetheless, the monosynaptic component of reflex discharges on the injured side did not recover. Dexamethasone treatment resulted in an increase in the number of BA-generating interneurons within the superficial dorsal horn laminae, and in a decrease in the proportion of units generating bursting activity (possibly of pathological nature).Neirofiziologiya/Neurophysiology, Vol. 27, No. 1, pp. 26–31, January–February, 1995.  相似文献   

5.
The effects on dorsal root potentials of applying dopamine to the perfusing fluid were investigated in experiments on a segment isolated from the spinal cord of 13- to 18-day-old rats. Dopamine induced slow, dose-dependent depolarization in motoneurons in 28 trials out of 32, retained in the solution blocking synaptic transmission. Threshold concentration of dopamine in the normal perfusing fluid measured 1·10–6 M and 1·10–5 M in a calcium-free perfusate containing magnesium or manganese ions. Depolarization was accompanied by an increased rate of motor discharges recorded from the ventral root. Segmental reflex response produced by dorsal root stimulation was depressed following depolarization. Hyperpolarization in response to dopamine was observed in 4 out of 32 experiments. Dopamine-induced electrotonic dorsal root potentials were suppressed by prior haloperidol application to the brain.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 19, No. 6, pp. 735–741, November–December, 1987.  相似文献   

6.
Different types of reflex discharges were produced in various preparations by stimulating the dorsal root of isolated frog spinal cord. These ranged from multiphasic low-amplitude waves to distinctly synchronized monosynaptic response. The discharges were followed by facilitation in the former and deep, protracted inhibition of response to test dorsal root stimulation in the latter. When interstimulus intervals measured 40–50 msec, inhibitory action was less pronounced than at shorter (15–30 msec) or longer (60–100 msec) intervals, thus indicating that at least two types of inhibition were at work, one at an earlier and the other at a later stage. Strychnine at a concentration of 10–5 M effectively reinforced the former and blocked the latter, while 10–4 M d-tubocurarine attenuated both types of inhibition substantially. It is concluded that inhibition of response occurs mainly as a result of recurrent activation of inhibitory systems via recurrent motoneuron axon collaterals when frog spinal cord afferents are excited. Intensity of the later (presynaptic) and earlier (postsynaptic) inhibition of reflex transmission is determined by the degree of synchrony in motoneuronal discharge in response to orthodromic stimulation.Institute of Medical Radiology, Academy of Medical Sciences of the USSR, Obninsk, Kaluga Oblast. Translated from Neirofiziologiya, Vol. 19, No. 3, pp. 343–350, May–June, 1987.  相似文献   

7.
Parameters of the reflex discharges evoked by spinal dorsal root stimulation were measured in rats with the sciatic nerve and spinal cord (at low thorasic level) transected five days earlier. Monosynaptic discharges in the ventral roots were found to increase after the operation; the degree of increase was significantly higher as compared with that observed after isolated transections of the spinal cord or the nerve. The combined lesion of the nerve and spinal cord could result in the appearance of high-amplitude reflex discharge components, probably of a polysynaptic nature. We concluded, from the comparison of modifications of reflex discharges, that the mechanisms underlying spinal hyperreflexia after nerve or spinal cord lesions differ considerably from each other.Neirofiziologiya/Neurophysiology, Vol. 26, No. 3, pp. 197–202, May–June, 1994.  相似文献   

8.
Microelectrode discharges of potentials have been realized from segmentary interneurons of the dorsal horn and intermediate nucleus of the spinal cord in cat at the L6–L7 level by electrical stimulation of the sensorimotor region of the brain cortex. It has been established that corticifugal influences on segmentary interneurons of the system of the flexor reflex and on neurons activated by high threshold muscle afferents (groups Ib, II, and III), or high threshold cutaneous afferents are predominantly excitatory. Interneurons activated by muscle afferents of group Ia or by the lowest threshold cutaneous fibers are weakly subjected to pyramidal influences. The mean latencies of excitatory postsynaptic potentials (EPSP's) and discharges evoked under the influence of pyramidal volley, for the neurons under study in the system of afferents of the flexor reflex are equal to 11.8±2.6 and 20.1±1.8 msec, respectively; for interneurons, excited only by high threshold muscle afferents, they are equal to 15.5±3.6 and 16.3±2.2 msec, respectively; and for interneurons, excited by high threshold cutaneous fibers they are equal to 11.8±2.6 and 18.3±1.4 msec, respectively. Possible pathways of activating segmentary interneurons from the lateral sensorimotor region of the brain cortex have been discussed.The A. A. Bogomolets Institute of Physiology, Academy of Sciences, Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 2, No. 1, pp. 17–25, January–February, 1970.  相似文献   

9.
The contribution of antidromic excitation of motoneurons to cord dorsum potentials (CDP) was studied in the spinal cord of anesthetized cats. It was shown that stimulation of ventral roots (VR) or peripheral nerves following deafferentiation of a number of segments by crosscutting of dorsal roots on the dorsal surface evokes appreciable positive-negative CDP (VR-CDP). Under intact conditions, VR effects of antidromic stimulation of efferent fibers brings appreciable input to the initial "fast" CDP component (the "afferent" peak); input values for the main mixed nerves of the hindlimb are presented. After conditioning stimulation of a mixed nerve, VR-CDP undergo inhibition with two maximums, associated with blocking of the effects of antidromic excitation of efferents by orthodromic mono- and polysynaptic reflex discharges of motoneurons. The hypothesis that intactness of efferents in nerves under stimulation can be determined from an analysis of initial CDP components is stated.Scientific-Research Institute of Biology, Dnepropetrovsk State University, Dnepropetrovsk. Translated from Neirofiziologiya, Vol. 23, No. 6, pp. 655–661, November–December, 1991.  相似文献   

10.
The effects of dopamine on ventral root potential produced by a single supramaximal dorsal root stimulation of the dorsal root was investigated during experiments on isolated superfused spinal cord segments from 10–16-day old rats. A reciprocal dose-dependent inhibition of the mono- and polysynaptic components of reflex response was also observed. Minimum effective concentration was 1×10–8 M dopamine. Extent of reflex response increased in step with dopamine concentration, so that the amplitude of the monosynaptic component of ventral root potential was decreased by 20% and 87% of baseline level by the action of 10–4 and 10–3 M dopamine respectively on the cord. The amplitude of the polysynaptic component was thereby decreased by an average of 18% and 87%. Findings indicate that dopaminergic brainstem-spinal pathways contribute to the governing of impulse transmission in the segmental reflex arcs. Inhibition of dopaminergic synaptic transmission probably underlies the increase in latency already described in the literature, as well as the increase observed in the threshold of reflex motor response to nociceptive action following either stimulation of the dopaminergic brainstem structures or intravenous administration of dopamine agonists.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 18, No. 5, pp. 616–621, September–October, 1986.  相似文献   

11.
Makii  E. A.  Rodinskii  A. G. 《Neurophysiology》2004,36(3):193-199
In albino rats, we studied the effects of long-lasting tetanization of the dorsal roots of the L 5 (homosynaptic activation) and L 4 (heterosynaptic activation) segments on reflex discharges in the L 5 ventral root evoked by single stimulation of the dorsal root of the same segment. Tetanization trains consisted of 5,000 stimuli applied with frequencies of 10, 50, 100, or 300 sec–1, and their effects were tested during 10 min. There were no long-term post-tetanic potentiation (PTP) of monosynaptic responses when low frequencies of homosynaptic tetanization (10 and 50 sec–1) were used. In the case of higher frequencies, PTP was rather clear and long-lasting. Under conditions of heterosynaptic activation, there was no PTP. Facilitation of polysynaptic responses developed at all the frequencies of homosynaptic tetanization used; when heterosynaptic tetanization was applied, such facilitation (although weaker) was also observed. In rats treated with agents increasing the excitability of spinal neuronal systems, such as thyroxine and 4-aminopyridine, tetanization of the studied inputs evoked long-term depression (LTD) of both mono- and polysynaptic components of the reflex discharges instead of PTP. Probable mechanisms of postsynaptic changes in the segmental reflex responses are discussed.  相似文献   

12.
The effects of glucocorticoid (dexamethasone) and mineralocorticoid (deoxycorticosterone) hormones on electrical excitability of nerve cells belonging to the dorsal and ventral horns of the spinal cord induced by stimulating the sciatic nerve, as well as background and evoked activity in single dorsal horn cells were investigated during experiments on adrenalectomized spinal rats using intracellular techniques for recording potential. Both hormones were found to produce mainly facilitatory effects in adrenalectomized animals, manifesting in increased background activity rates in single cells and higher amplitude of field potentials in nerve cells of the dorsal half of the spinal cord. It was shown that neuronal response followed different patterns in the ventral half of the spinal cord gray matter under the action of gluco- and mineralocorticoids: dexamethasone and deoxycorticosterone respectively increased and reduced the amplitude of field potentials in the motoneuronal region. Findings indicate the modulatory influence of adrenal cortical hormones on the electrical activity of spinal cord neurons.Institute of Experimental Biology, Academy of Sciences of the Armenian SSR, Erevan. I. A. Orbeli Academy of Sciences of the Armenian SSR, Erevan. Translated from Neirofiziologiya, Vol. 21, No. 2, pp. 233–238, March–April, 1989.  相似文献   

13.
The distribution of myelinated and nonmyelinated nerve fibers of the saphenous nerve of cats in the ventral and dorsal roots of the spinal cord was investigated by methods improving the signal—noise ratio in records of evoked responses from the nerve. The fibers of this nerve enter the spinal cord through roots of segments L4–6. Nerve fibers with conduction velocities of between 80 and 0.38 m/sec were distributed in the dorsal roots of these segments. Four groups of nerve fibers with conduction velocities of 80–60, 40–30, 12.0–3.0, and 1.1–0.51 m/sec, possibly afferent in nature, were found in the ventral roots. The conditions of origin and detection of low-amplitude potentials in the roots of the spinal cord and the probable functional role of the nerve fibers in the ventral roots are discussed.Research Institute of Applied Mathematics and Cybernetics, N. I. Lobachevskii State University, Gor'kii. Translated from Neirofiziologiya, Vol. 7, No. 6, pp. 647–654, November–December, 1975.  相似文献   

14.
Using the method of microelectrode (intracellular and extracellular) recording, the mechanism of inhibition following reflex discharge in interneurons of the lumbosacral section of the spinal cord of cats on activation of cutaneous and high-threshold muscle afferents was studied. It was shown that the postdischarge depression of the reflex responses 10–20 msec after the moment of activation of the neuron is due to afterprocesses in the same neuron and presynaptic pathways. The depression of spike potentials from the 20th to the 100th msec is produced by inhibitory postsynaptic potentials (IPSP). During the development of IPSP the inhibition of spike potentials can be due to both a decrease of the depolarization of the postsynaptic membrane below the critical threshold and a decrease of sensitivity of the cell membrane to the depolarizing action of the excitatory postsynaptic potential (EPSP). At intervals between the stimuli of 30–100 msec the duration of EPSP after the first stimulus does not differ from that after the second stimulus. Hence, it is suggested that the presynaptic mechanisms do not play an essential part in this type of inhibition of interneurons. The inhibition following the excitation favors the formation of a discrete message to the neurons of higher orders.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. Translated from Neirofiziologiya, Vol. 2, No. 1, pp. 3–9, January–February, 1970.  相似文献   

15.
Synaptic potentials of smooth muscles of the gastrointestinal tract arising in response to intramural stimulation were studied by intracellular recording of potentials and the sucrose gap method. The results showed that muscarinic cholinergic neuromuscular transmission in smooth-muscle cells of the gastrointestinal tract is purely excitatory. This transmission is most marked in the fundal part of the stomach. Adrenergic control of motor activity is manifested as excitation and inhibition of smooth muscles. Relations between these phenomena differ in different parts of the gastrointestinal tract. Depression of inhibitory adrenergic effects by apamin discloses excitation of smooth muscles which is not found under ordinary conditions. Like its inhibitory action, the excitatory action of noradrenalin is exerted as a result of activation of -adrenoreceptors. Nonadrenergic synaptic inhibition, which is more effective than adrenergic, is found in smooth-muscle cells of the circular layer of all parts of the gastrointestinal tract studied. Inhibitory postsynaptic potentials consists of two components: a first fast, and a second slow. Apamin blocks mainly the first phase of the synaptic response. During inhibition of nonadrenergic inhibitory postsynaptic potentials by apamin, noncholinergic synaptic excitation resistant to the action of blockers of cholinergic, adrenergic, and serotoninergic transmission is found in smooth muscles of the cecum. It is complex in character in this part of the intestine: an initial excitatory postsynaptic potential and a slow late depolarization wave. In smooth-muscle cells of other parts noncholinergic excitation is manifested only as a slow depolarization wave. The following types of synaptic influences of the autonomic nervous system on smooth-muscle cells of the gastrointestinal tract are therefore postulated: nonadrenergic excitatory, both cholinergic and noncholinergic; nonadrenergic inhibitory, adrenergic excitatory and adrenergic inhibitory, and also presynaptic modulation of neuromuscular transmission.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 16, No. 3, pp. 307–319, May–June, 1984.  相似文献   

16.
In experiments carried out on the isolated spinal cord of the tortoise Emys orbicularis postsynaptic potentials produced in spinal motoneurons by stimulation of the descending tracts and dorsal roots were investigated by means of the intracellular recording technique. Postsynaptic potentials were completely and reversibly blocked in Ca2+-free solutions containing 5.0 mM Mg2+ or 2.0 mM Mn2+. The amplitude and frequency of spontaneous synaptic potentials were also reduced under these conditions. The effect of Ca2+-free medium indicates that the synaptic transmission in these synapses is mediated by chemical mechanism.  相似文献   

17.
During experiments on an isolated segment of the spinal cord of 2- to 3-week-old rats, a study was made of the effects of vasopressin and oxytocin on the activity of dorsal horn cells produced by stimulating the afferent root. Both field and action potentials were recorded in single cells. It was established that vasopressin and oxytocin produced reversible inhibition of the postsynaptic component of field potentials. The amplitude of potentials was reduced by 33–39% by vasopressin and by 12–34% using oxytocin. The effect of the test substances depended on the concentration used and the duration of their action on the brain. Both vasopressin and oxytocin reversibly depressed discharges of single dorsal horn cells evoked by stimulating the dorsal root. These two neuropeptides prolonged latency, and reduced the number of evoked potentials or completely suppressed response. A facilitatory effect was recorded in a small number of cells. We deduced from our findings that their hypothalamospinal neurohormonal system inhibits transmission of afferent impulses at the level of interneurons of the dorsal horn.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 17, No. 5, pp. 634–640, September–October, 1985.  相似文献   

18.
In experiments on cats, we investigated focal potentials of Clarke's column neurons and discharges of individual neurons recorded extracellularly. An ultrasonic scalpel was used to remove the part of the spinal cord between Th13 and L3, and an electrode was inserted into the face of the caudal segment of the spinal cord along the axis of Clarke's column. Orthodromic excitation of Clarke's column neurons was evoked by stimulating cut nerves of the ipsilateral extremity; antidromic excitation was evoked by stimulating the dorsolateral funiculus, which was preliminarily separated from the removed portion of the spinal cord. It was found that the orthodromic potential, antidromic potential, and discharges are distinctly registered when the method of electrode insertion is used, whereas they were not recorded when the microelectrodes were sunk into the dorsal surface in these experiments. It is demonstrated that orthodromic and antidromic focal potentials of Clarke's column neurons are similar to motoneuron focal potentials with respect to time characteristics. Inversion of the charge sign was recorded with the approach of the microelectrode's tip to the soma of Clarke's column neurons. It is hypothesized that the success of recording focal potentials and extracellular discharges of Clarke's column neurons resulted from the fact that the orientation of dendrites of these cells matches the direction of microelectrode movement. The slender portion of the microelectrode penetrates the interdendritic space, where tension of the extracellular field is the greatest; it then moves through this space to reach the soma.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad; A. A. Bogomol'ets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 2, No. 5, pp. 528–535, September–October, 1970.  相似文献   

19.
Presynaptic inhibition of primary afferents can be evoked from at least three sources in the adult animal: 1) by stimulation of several supraspinal structures; 2) by spinal reflex action from sensory inputs; or 3) by the activity of spinal locomotor networks. The depolarisation in the intraspinal afferent terminals which is due, at least partly, to the activation of GABA(A) receptors may be large enough to reach firing threshold and evoke action potentials that are antidromically conducted into peripheral nerves. Little is known about the development of presynaptic inhibition and its supraspinal control during ontogeny. This article, reviewing recent experiments performed on the in vitro brainstem/spinal cord preparation of the neonatal rat, demonstrates that a similar organisation is present, to some extent, in the new-born rat. A spontaneous activity consisting of antidromic discharges can be recorded from lumbar dorsal roots. The discharges are generated by the underlying afferent terminal depolarizations reaching firing threshold. The number of antidromic action potentials increases significantly in saline solution with chloride concentration reduced to 50% of control. Bath application of the GABA(A) receptor antagonist, bicuculline (5-10 microM) blocks the antidromic discharges almost completely. Dorsal root discharges are therefore triggered by chloride-dependent GABA(A) receptor-mediated mechanisms; 1) activation of descending pathways by stimulation delivered to the ventral funiculus (VF) of the spinal cord at the C1 level; 2) activation of sensory inputs by stimulation of a neighbouring dorsal root; or 3) pharmacological activation of the central pattern generators for locomotion evokes antidromic discharges in dorsal roots. VF stimulation also inhibited the response to dorsal root stimulation. The time course of this inhibition overlapped with that of the dorsal root discharge suggesting that part of the inhibition of the monosynaptic reflex may be exerted at a presynaptic level. The existence of GABA(A) receptor-independent mechanisms and the roles of the antidromic discharges in the neonatal rat are discussed.  相似文献   

20.
Convergence of contralateral somatic afferent synaptic influences on segmental inhibitory neurons was investigated by intracellular recording of postsynaptic potentials of -motoneurons in experiments on cats. Excitatory synaptic influences of afferents of the contralateral flexor reflex were shown to converge on interneurons of both segmental inhibitory systems studied: afferents of flexor reflex and group Ia muscle afferents. Interneurons of inhibitory systems are exposed not only to excitatory but also to inhibitory contralateral influences. Contralateral inhibitory PSPs of montoneurons are produced through ipsilateral inhibitory systems; a leading role is played by inhibitory neurons of the flexor reflex system of afferents. Inhibitory neurons of the Ia system as a rule do not make an important contribution to generation of contralateral IPSPs.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. Translated from Neirofiziologiya, Vol. 5, No. 5, pp. 476–484, September–October, 1973.  相似文献   

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