Mismatch between temperature preferences and morphology in F1 hybrid newts (Triturus carnifex×T. dobrogicus)

1. The influence of interspecific hybridization on temperature preferences and morphology was examined in newts, Triturus carnifex and Triturus dobrogicus, before and after metamorphosis.

2. Thermoregulatory behavior was measured in an aquatic thermal gradient (5–32.5 °C) during 24 h.

3. Hybrid temperature preferences were similar to preferences of maternal species in both premetamorphic larvae and recently metamorphosed individuals.

4. Hybrid morphology (i.e., forelimb length and axilla–groin distance) was intermediate relative to parental species.

5. The mismatch between morphology and thermal preference in hybrid phenotypes indicates potential hybrid disadvantage in both intermediate and parental habitats.

Temperature-mediated morphology changes during metamorphic climax in the African clawed frog, Xenopus laevis

(1) Investigations of the effect of temperature on body size are largely limited to the larval phase, with our understanding of the effect of temperature during metamorphic climax entirely restricted to the insects.

(2) Environmental temperature was manipulated only during metamorphosis in the aquatic amphibian Xenopus laevis.

(3) Lower temperatures during metamorphosis resulted in individuals with greater mass, head width and snout–vent length on the completion of metamorphosis.

(4) This suggests that temperatures experienced during the relatively short metamorphic phase will play an important part in determining the temperature–size relationship in amphibians.

Correlated evolution of thermal characteristics and foraging strategy in lacertid lizards

1. We investigated the association between field body temperatures (T_b), field air temperatures (T_a), and their differences (Δ) with measurements of foraging activity (percentage of time moving (PTM), number of movements per minute (MPM) and proportion of prey attacked while moving (PAM)) for 25 species of lacertid lizards.

2. Lizards active at relatively high field body temperatures tended to have higher PTM and PAM values. We found no association between temperatures and MPM. The difference Δ did not co-vary with PTM and MPM, but showed a positive trend with PAM.

3. Our results seem robust with regard to the assumptions of different models of evolution and to the phylogenetic trees used.

Effect of temperature on the electrical activity of the rat epididymis in vitro

1. 1.|Regional differences in the frequency of electrical activity in rat epididymis were maintained at all temperatures below 39°C.

2. 2.|The change in frequency per deg C increased with temperature and was highest in the temperature region of 34–39°C and the Arrhenius plots of the frequency were linear and parallel in all parts of the epididymis.

3. 3.|The Q₁₀ of the frequency varied between 2.2.–4.3.

4. 4.|The conduction velocity at the cauda epididymis was highest (2.8 mm/s) at 37°C. The Q₁₀ of the conduction velocity was 2.3 in the temperature region of 24–37°C.

Serum catecholamine and hormonal titers in the hibernating snake Naja haje haje, with reference to the annual climatic cycle

(1) The analysis of catecholamines revealed alterations in norepinephrine levels in the blood serum and the central nervous system of the Egyptian cobra during the different phases of the hibernation cycle. This may reflect an increased central demand for norepinephrine as a mediator for adaptive changes to cold.

(2) The low and variable concentration of dopamine suggests retarded synthesis during all the phases studied.

(3) Serotonin may be depleted or associated with increased activity of serotonergic neurons during cold acclimation. This increases the rate of turnover of serotonin to 5-hydroxy indole acetic acid.

(4) Cricannual measurements of cortisol and adrenocorticotrophic hormones revealed marked suppression that would clarify the direct effect of hibernation on higher centers. The decreased levels during prehibernation and hibernation were restored in normothermic animals when the pituitary–adrenal axis resumed its activity.

(5) The decline in thyroid hormone concentrations has its behavioural and physiological responses that lead to seasonal adjustments in the hibernating cobras.

Ethanol effects on temperature-sensitive hypothalamic neurons in rat brain slices

1. We recorded impulse activity of thermosensitive hypothalamic neurons in rat brain slices during superfusion with ethanol at constant temperatures and during slow sinusoidal temperature changes.

2. At constant temperatures of 37 °C, ethanol application typically induced a triphasic change of the firing rate: An initial excitation turned into complete inhibition followed by spontaneous recovery to higher firing rates.

3. Ethanol application increased the neurons’ temperature sensitivity remarkably.

4. Our data indicate complex neuromodulatory effects of ethanol with different time delays which interfere with basic mechanisms of temperature transduction.

Heat flow of the paraplegic and able-bodied lower limb during resting heat exposure

1. The heat flow of paraplegic (PA) and able-bodied (AB) subjects were determined at rest in cool and warm conditions.

2. During heat exposure upper body sites for both groups showed heat loss, whereas the lower body sites of the PA groups showed heat gain.

3. During heat exposure, a systematic difference between groups in the relationship between heat flow and calf-skin temperature existed.

4. In conclusion, heat storage appears to be localised in PA subjects at rest and centralised for AB subjects.

Central thermosensitivity and the integrative responses of hypothalamic neurons

1. Hypothalamic thermosensitive neurons are important in the integrative regulation of body temperature. In addition to receiving afferent thermal input, their activity correlates with the stimulation of thermoregulatory mechanisms.

2. While cold sensitivity is synaptically mediated, warm sensitivity depends on inherent cellular responses. Warm sensitive neurons may also respond to other homeostatic variations, hormones, and endogenous mediators.

3. In contrast to peripheral thermoresponsiveness, which depends on conductance changes that regulate membrane potential, hypothalamic warm sensitivity relies on regulating the prepotential phase of the action potential.

4. Additionally, the recent morphologic characterization of these neurons supports the criterion for warm sensitivity.

Thermoregulatory effects of central injection of noradrenaline in the new-born columbian ground squirrel (Spermophilus columbianus)

1. 1.Effects of centrally injected noradrenaline (NA) into new-born (12–300 h. post-partum) Columbian ground squirrels (Spermophilus columbianus) were studied to provide comparative data on ontogeny of the thermoregulatory pathways in a hibernating species.

2. 2.At warm ambient temperatures (32–34°C, similar to nest temperature), NA increased heat production (47–92%). rectal temperature (0.27–1.73°C), and axillary temperature (0.59–1.92°C). Peak magnitudes of heat production increased with increasing age on a per unit weight basis.

3. 3.At lower temperatures (28–31°C), NA had no effect on heat production.

4. 4.The data indicate that metabolic and thermal responses to NA in neonates of hibernating species are comparable (e.g. rabbit. guinea pig) or different (e.g. lamb) from those observed in neonates of non-hibernating species.

Effect of hyperthermia on blood constituents in the domestic rabbit (Lepus cuniculus)

1. 1. Effects of exposing rabbits to temperatures 37–50°C on body-core temperature and some blood constituents were investigated.

2. 2. Heat stroke death occurred at or above a critical core-temperature of 43.0°C.

3. 3. Plasma osmolality and levels of glucose, urea and lactate were significantly elevated in hyperthermia.

4. 4. Widespread tissue damage was indicated by increases in plasma activities of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and creatinine phosphokinase (CPK).

5. 5. The most sensitive indicators of impending heat stroke in heat stressed rabbits were plasma levels or urea, lactate and CPK.

Strategies of social wasps for thermal homeostasis in light paper nests

1. With the derived free-run temperature of light paper nests the influence of the thermal capacity of the nest or the nest site, respectively, on thermal homeostasis is quantified.

2. Gravimetric information from the continuous investigation of the nest weight coupled with temperature and humidity measurement allows the correlation of the colonies’ foraging behavior with their heat production inside the nest.

3. Wasps (Vespa crabro) do not regulate their nest environment towards a specific and constant temperature but rather build nests adapted to their foraging behavior and general energy budget.

In-vitro heat exchange at the rete of the Boer goat under specified laboratory conditions

1. Each half rete from five Boer goats was perfused with water at 38 °C and flow rate of 2 ml min⁻¹ while simultaneously perfusing the cavernous sinus with water at different temperatures and flow combinations and recording temperatures across the rete.

2. The minimum temperature difference across the rete was recorded at a cavernous sinus perfusion temperature of 37.8 °C and flow rate of 2 ml min⁻¹.

3. Slopes of heat exchange increased threefold when the flow was increased four times.

4. These results support the idea that the rete is an obligate heat exchanger.

Thermal responses and temperature tolerance of a dessert ant-lion larva

1. 1.Cueta trivirgata larvae construct pits in the dry Kuiseb River bed in the Namib Desert.

2. 2.An art, Ocymyrmex robustior comprises 65.4% of the biomass of prey consumed by the ant-lions.

3. 3.O. robustior is active between surface temperatures of 27–68°C.

4. 4.Ant-lions tolerate high body temperatures (LD₅₀ = 53.4°C).

5. 5.By exploiting the pit microclimate and by digging below the surface during extreme thermal loads, ant-lions can capture prey at surface temperatures of 13–63°C.

6. 6.These behavioural and physiological adaptations enable ant-lions to maximize the duration of vigilance and hence prey capture success.

Body temperature measurements in the clinic; evaluation of practice in a Norwegian hospital

(1) The aim of this study was to determine the degree of agreement between the requirements of medical staff for body temperature measurements for clinical use, as defined in a previous study, and current practice in two departments in a Norwegian university hospital.

(2) The results showed that few of the highest-ranked requirements for body temperature measurements were regarded by health personnel as having been fulfilled.

(3) It will be necessary to change current practice in order to obtain agreements between these requirements and the performance of temperature measurements. This will help to improve the basis for diagnosis and treatment and provide more efficient body temperature measurement.

Electrical skin resistance and rectal temperature changes in resting human subjects exposed to heat

1. 1.|Fourteen male volunteers were examined under passive heating.

2. 2.|Electrical skin resistance (ESR) and rectal temperature (T_re) were measured during the whole period of exposure.

3. 3.|It was found that:

• —|ESR decreases rapidly with increasing air temperature. Assuming an exponential curve yields a mean time constant of 14 min.

• —|There is a correlation between the individual ESR time constants and T_re increases (r = 0.695, P < 0.005).

• —|Additional changes of ESR were noted in 8 subjects at a constant air temperature of 42°C.

4. 4.|It is concluded that ESR may be a useful indicator of the sweating response of the human thermoregulatory system during exogenous heat load.

Glutathion content, rate of apoptosis, and brown adipose tissue mass in rats exposed to different ambient temperatures

1. Amount of glutathione (GSH) in the interscapular brown adipose tissue (IBAT) of cold-adapted (5±1 °C), and 1, 3 and 7 days re-adapted (22±1 °C) rats were examined.

2. The higher amount of GSH was found in the IBAT of cold-adapted rats compared to control animals (22±1 °C).

3. After 1 and 7 days of re-adaptation, the level of GSH was still significantly higher compared to control group.

4. On the third day of re-adaptation, the quantity of GSH in the tissue was significantly lower compared to the cold-adapted group.

5. In cold-adapted animals, intensity of apoptosis in IBAT was lower, whereas in re-adapted animals (3rd and 7th day) it was higher than in control group.

6. This study indicates that GSH depletion is associated with an enhanced apoptosis in IBAT during re-adaptation.

Seasonal changes and wind dependence of thermal conductance in dorsal fur from two small mammal species (Peromyscus leucopus and Microtus pennsylvanicus)

(1) We measured the thermal conductance of dorsal pelage from meadow voles (Microtus pennsylvanicus) and white-footed mice (Peromyscus leucopus) during summer and winter.

(2) Thermal conductance was lower in the winter pelage of both species, but the seasonal change was greater in meadow voles.

(3) The form of wind speed dependence was determined by fitting a nonlinear curve of the form a+bu^c to data recorded at five wind speeds. The most appropriate exponent c was between 0.908 and 0.987, depending on species and season. These values are common and suggest that thermal and dynamic forces are important.

Activation of thermoregulatory control elements in precocial birds during the prenatal period

1. Heat production (HP) and body core temperature (CT) were measured in the last third of incubation period in embryos of domestic fowl (Gallus gallus) as well as Muscovy ducks (Cairina moschata). Breathing activity and blood flow in the chorioallantoic membrane were simultaneously estimated after internal pipping.

2. The results revealed that ambient temperature (T_a) affected HP and CT, thus manifest endothermic responses starting on D18 of incubation in Gallus gallus and on D20 in Cairina moschata.

3. In comparison to HP heat loss mechanisms are well developed during the embryonic period. Panting occur after internal pipping. By this and by vasodilatation of the chorioallantoic vessels the heat loss to the environment may be increased at high T_a. Both factors may protect the organism against overheating during a relative long period.

4. Some general rules concerning the mechanism of functional embryonic development are formulated.

Mechanisms of heat protection by cycloheximide or puromycin: Involvement of polyamines?

1. 1.|When Chinese hamster ovary cells are treated with cycloheximide (10 μg/ml) or puromycin (100 μg/ml) for 2 h before and during heating at 43°C for 3 h, there is protection from hyperthermic killing; i.e. the plating efficiency increases 2000-fold from 3.7 × 10⁻⁵ to (6–9) × 10⁻².

2. 2.|The total intracellular levels of spermidine and spermine are not altered by the hyperthermic or drug treatments.

3. 3.|The small 30% decrease in intracellular putrescine observed after heating is not altered by drug treatment.

4. 4.|Heat protection by treatment with cycloheximide or puromycin cannot be attributed to changes in levels of total intracellular polyamines.

Study of function of fingers by physiological tremor

1. 1. Physiological tremor is invisible mechanical vibration of body parts.

2. 2. It has two peak frequencies in the p ower spectrum. The origin of the peaks has not been well elucidated. Therefore, the mechanism and its application to labor science is studied in the paper.

3. 3. The effect of immersion of fingers in water and of loading weights on fingers are performed to elucidate the mechanism involved.

4. 4. It is found that the power spectrum output has two bands from the lower frequency band (1.5–18 Hz) from the CNS and the higher frequency band (18–50 Hz).

5. 5. These results are applied to the evaluation of fatigue of fingers in tapping and typing work.

6. 6. The total power, which is the sum of power spectrum in frequency range between 1.5 and 50 Hz, is employed as evaluational index. The change of total power explains finger fatigue during tapping and typing loads.

7. 7. Physiological tremor is an effective way of evaluating the function of fingers in human work.