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1.
To investigate salt stress and biochar application effects on nodulation and nitrogen metabolism of soybeans (Glycine max cv. M7), an experiment was conducted under the control condition. The treatments comprised three biochar rates (non, 50 and 100 g kg?1 soil) and three salinities (0, 5 and 10 dS m?1 NaCl), with four replications of treatments. Salt stress diminished the number of nodules and their weights in the soybean roots. Nitrogen content and metabolism decreased in nodules, roots and shoots, while reducing the activity of glutamate dehydrogenase (GDH), glutamine synthetase (GS), glutamine oxoglutarate aminotransferase (GOGAT) and nitrate reductase (NR). Also, salinity brought down root and shoot weight, total plant biomass, chlorophyll content, leaf area (LA) and rubisco activity in the soybean. On the other hand, application of biochar improved nodulation, nitrogen content, rubisco activity, GDH, GS, GOGAT and NR activities in different parts of the soybean and nodules under salt stress, and consequently improved chlorophyll content, LA, root and shoot weight. Both the 50 and 100 g kg?1 biochar rates showed similar effects in improving nitrogen metabolism and plant performance under salt stress. Generally, biochar increased nodulation and nitrogen metabolism of the soybean under saline conditions. 相似文献
2.
外源亚精胺对高温胁迫下黄瓜幼苗氮素代谢的影响 总被引:3,自引:0,他引:3
以较为耐热的黄瓜品种‘津春4号’为试材,在人工气候箱中,采用石英砂培加营养液浇灌的栽培方式,研究了外源亚精胺( Spd)对高温胁迫(42℃)下黄瓜幼苗氮素代谢的影响.结果表明:短期高温胁迫处理,尤其是4h内,植株硝态氮含量降低而铵态氮含量升高;外源Spd预处理使幼苗体内硝态氮和铵态氮含量升高且硝酸还原酶(NR)活性增强.较长期高温胁迫处理下,幼苗根系中硝态氮含量升高但向地上部运输受阻,根系NR钝化,根系和叶片中铵态氮含量均显著升高;高温胁迫下喷施Spd,除进一步促进根系吸收硝态氮且向地上部运输外,根系和叶片NR活性亦有所升高,从较长期的效果看,外源Spd还具有防止铵态氮过度积累、促进幼苗体内氮素代谢趋于正常的作用. 相似文献
3.
为研究纯化腐植酸(PHA)在不同水平氮胁迫下对黄瓜植株生长和氮代谢的影响,探明PHA对逆境胁迫的缓解作用机制,采用水培方式,选用新泰密刺为供试品种,以正常氮水平(11 mmol·L-1 NO3-)为对照,进行低氮(1.0 mmol·L-1 NO3-)和高氮(101 mmol·L-1 NO3-)胁迫处理,研究纯化腐植酸对氮胁迫下黄瓜幼苗生长和氮代谢的影响.结果表明: 低氮和高氮胁迫均抑制了黄瓜幼苗生长,株高、茎粗、叶面积、干物质积累量均低于正常氮水平处理.施用纯化腐植酸促进了正常氮水平及低氮胁迫下黄瓜干物质积累,在高氮胁迫下差异不显著.PHA影响了黄瓜幼苗NO3-的吸收,呈低氮下促进、高氮下抑制吸收的趋势;PHA显著降低了低氮、高氮胁迫下根系和叶片中的铵态氮含量;与正常氮水平相比,低氮、高氮胁迫下根系和叶片的硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸合酶(GOGAT)、谷氨酸脱氢酶(GDH)活性及根系中亚硝酸还原酶(NiR)活性均显著降低,PHA不同程度地提高了NR、NiR、GS、GOGAT、GDH活性,还提高了根系和叶片中游离氨基酸、可溶性蛋白的含量.综上,添加PHA缓解了氮胁迫对黄瓜幼苗生长的抑制作用. 相似文献
4.
Salicylic acid (SA) is known to affect photosynthesis under normal conditions and induces tolerance in plants to biotic and abiotic stresses through influencing physiological processes. In this study, physiological processes were compared in salt-tolerant (Pusa Vishal) and salt-sensitive (T44) cultivars of mungbean and examined how much these processes were induced by SA treatment to alleviate decrease in photosynthesis under salt stress. Cultivar T44 accumulated higher leaf Na+ and Cl− content and exhibited greater oxidative stress than Pusa Vishal. Activity of antioxidant enzymes, ascorbate peroxidase (APX) and glutathione reductase (GR) was greater in Pusa Vishal than T44. Contrarily, activity of superoxide dismutase (SOD) was greater in T44. The greater accumulation of leaf nitrogen and sulfur through higher activity of their assimilating enzymes, nitrate reductase (NR) and ATP-sulfurylase (ATPS) increased reduced glutathione (GSH) content more conspicuously in Pusa Vishal than T44. Application of 0.5 mM SA increased nitrogen and sulfur assimilation, GSH content and activity of APX and GR. This resulted in the increase in photosynthesis under non-saline condition and alleviated the decrease in photosynthesis under salt stress. It also helped in restricting Na+ and Cl− content in leaf, and maintaining higher efficiency of PSII, photosynthetic N-use efficiency (NUE) and water relations in Pusa Vishal. However, application of 1.0 mM SA resulted in inhibitory effects. The effect of SA was more pronounced in Pusa Vishal than T44. These results indicate that SA application alleviates the salt-induced decrease in photosynthesis mainly through inducing the activity of NR and ATPS, and increasing antioxidant metabolism to a greater extent in Pusa Vishal than T44. 相似文献
5.
Carla G. Zilli Karina B. Balestrasse Gustavo G. Yannarelli Ariel H. Polizio Diego M. Santa-Cruz María L. Tomaro 《Environmental and Experimental Botany》2008,64(1):83-89
The behaviour of enzymes involved in nitrogen metabolism, as well as oxidative stress generation and heme oxygenase gene and protein expression and activity, were analysed in soybean (Glycine max L.) nodules exposed to 50, 100 and 200 mM NaCl concentrations. A significant increase in lipid peroxidation was found with 100 and 200 mM salt treatments. Moreover, superoxide dismutase, catalase and peroxidase activities were decreased under 100 and 200 mM salt. Nitrogenase activity and leghemeoglobin content were diminished and ammonium content increased only under 200 mM NaCl. At 100 mM NaCl, glutamine synthetase (GS) and NADH-glutamate dehydrogenase (GDH) activities were similar to controls, whereas a significant increase (64%) in NADH-glutamate synthase (GOGAT) activity was observed. GS activity did not change at 200 mM salt treatment, but GOGAT and GDH significantly decreased (40 and 50%, respectively). When gene and protein expression of GS and GOGAT were analysed, it was found that they were positively correlated with enzyme activities. In addition, heme oxygenase (HO) activity, protein synthesis and gene expression were significantly increased under 100 mM salt treatment. Our data demonstrated that the up-regulation of HO, as part of antioxidant defence system, could be protecting the soybean nodule nitrogen fixation and assimilation under saline stress conditions. 相似文献
6.
外源甜菜碱对盐胁迫下枸杞光合功能的改善 总被引:59,自引:4,他引:59
研究了外源甜菜碱对盐胁迫下枸杞扦插苗叶片光合功能的影响。结果表明,外源甜菜碱能使盐胁迫下的枸杞叶片叶绿素荧光动力学参数Fo、Fm、Fv、Fv/Fm、Fm/Fo和Fv/Fo增高,使光合色素叶绿素a(Chla)、叶绿素b(Chlb)和类胡萝卜素(Car)含量明显增加,叶绿素a与b的比值(Chla/Chlb)升高,类胡萝卜素与叶绿素的比值(Car/Chl)降低,说明外源甜菜碱有利于植物对光能的捕获、吸收、传递和转换,提高叶片的光合活性,降低盐胁迫对植物的抑制作用。 相似文献
7.
盐胁迫对黄瓜幼苗根系生长和多胺代谢的影响 总被引:11,自引:3,他引:11
以两个不同抗盐性黄瓜品种为试材,采用营养液水培法,研究了NaCl胁迫对幼苗根系生长和多胺代谢的影响.结果表明:盐胁迫下黄瓜幼苗根系生长受抑制,膜脂过氧化和电解质渗漏升高,而弱抗盐品种‘津春2号'的变化幅度大于抗盐品种‘长春密刺';盐胁迫下‘长春密刺'根系精氨酸脱羧酶、鸟氨酸脱羧酶和S-腺苷蛋氨酸脱羧酶活性升高幅度均大于‘津春2号',其最高值分别比对照增加了149.3%、60.1%、69.4%和118.6%、56.2%、50.6%;'长春密刺'多胺氧化酶活性升高幅度小于‘津春2号',而二胺氧化酶活性仅在‘长春密刺'中增加.'长春密刺'根系游离态亚精胺和精胺、结合态和束缚态多胺含量均显著增加,而‘津春2号'根系游离态腐胺含量显著增加.表明黄瓜根系中较高的游离态亚精胺和精胺、结合态和束缚态多胺以及较低的游离态腐胺含量有利于提高幼苗对盐胁迫逆境的适应能力. 相似文献
8.
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10.
热带林下人工种植阳春砂仁的生长与果实产量动态 总被引:45,自引:3,他引:42
调查了西双版纳不同海拔热带沟谷雨林和次生林下的阳春砂仁生长和果实产量动态.结果表明,西双版纳热带林下阳春砂仁自身年龄增长、林下光照不足和旱季水分胁迫影响阳春砂仁果实产量.随种植期增加,阳春砂仁果实产量和成熟植株密度降低.当林下光照水平在全日照的35%以下时,阳春砂仁果实产量随林下日照水平变化呈线性增加(P<0.05).沟谷下方阳春砂仁果实产量显著高于上方(P<0.05).海拔600~1000m,由于阳春砂仁的主花期从干热季3~4月推迟到雨季5月,果实产量显著增加.沟谷雨林和次生林下阳春砂仁果实产量差异不显著.因此,在海拔800~1000m沟谷中轮歇地次生林下有计划种植阳春砂仁,代替在沟谷雨林下种植阳春砂仁,既能解决沟谷雨林下光照和当地旱季水分不足对阳春砂仁果实产量的影响,又有利于热带沟谷雨林的保护. 相似文献
11.
G. Chen P. S. Fan W. M. Feng A. Q. Guan Y. Y. Lu Y. L. Wan 《Russian Journal of Plant Physiology》2017,64(1):116-123
The effects of foliar spray application of 5-aminolevulinic acid (ALA) on the growth, nitrogen metabolism, and ion distribution of salt-stressed watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) seedlings were investigated. Supplementation of the nutrient solution with 100 mM NaCl significantly reduced leaf and root biomass of watermelon plants. Foliar application of 1.25 mM ALA significantly alleviated the inhibition of plant growth under salt stress. Salinity induced significant accumulation of nitrate, ammonium, and soluble protein and a significant decrease in the activities of nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate synthase (GOGAT), and glutamate dehydrogenase (GDH) in watermelon plants. However, ALA significantly increased the activities of NR, GS, GOGAT, and GDH, but decreased the ammonium content and NiR activity. In addition, salt stress resulted in significant accumulation of Na+ and Cl? in plants, but decreased the contents of K+ and Mg2+. Application of ALA alleviated the salt stress-induced ion toxicity, and increased the contents of K+ and Mg2+. ALA also increased soluble protein and proline contents in salt-stressed watermelon plants. These results indicated that application of ALA alleviated the accumulation of Na+ and Cl? in salt-stressed watermelon plants, especially through regulating nitrogen metabolism and ion distribution, which were associated with an improvement in plant growth. 相似文献
12.
盐胁迫下外源NO对苜蓿幼苗生长及氮代谢的影响 总被引:1,自引:0,他引:1
为探寻增强苜蓿耐盐能力的调控途径,以甘农4号苜蓿品种为材料,采用NO供体硝普钠、NO清除剂c-PTIO及硝普钠类似物亚铁氰化钠处理苜蓿幼苗,研究盐胁迫下外源NO对苜蓿幼苗生长、光合特征、氮同化酶活性和氮代谢物含量的影响.结果表明: 外源NO能明显缓解盐胁迫对苜蓿幼苗生长及光合作用的抑制,单株干质量、叶绿素含量、净光合速率、蒸腾速率和可溶性蛋白含量增加;外源NO能增强硝酸还原酶、谷氨酰胺合成酶和谷氨酸合酶活性,抑制蛋白水解酶和谷氨酸脱氢酶活性, 降低叶片中游离氨基酸含量,提高硝态氮含量,加快铵的同化.NO供体SNP的类似物亚铁氰化钠对盐胁迫下苜蓿幼苗生长及氮代谢无调控作用;施用NO清除剂c-PTIO加剧了盐胁迫对苜蓿幼苗生长和氮代谢的抑制,添加外源NO能缓解c-PTIO的抑制效应.盐胁迫下,外源NO和内源NO均参与了苜蓿幼苗氮代谢的调控. 相似文献
13.
Hydroponically grown cucumber plants were exposed to 14-d period of salinity (0, 50, 100 mM NaCl). NaCl caused reduction in
the relative water content in the leaves. The Na+ content increased and the K+ content decreased. The net photosynthetic rate, stomatal conductance and transpiration rate were markedly decreased by all
of the salt treatments. Salinity decreased also the maximum quantum efficiency of photosystem 2 (PS 2) determined as the variable
to maximum fluorescence ratio, the photochemical quantum yield of PS 2 and the photochemical fluorescence quenching, while
the non-photochemical quenching increased. Above results indicate that NaCl affects photosynthesis through both stomata closure
and non-stomatal factors. 相似文献
14.
土壤紧实胁迫对黄瓜根系生长及氮代谢的影响 总被引:3,自引:0,他引:3
用容重分别为1.25 g·cm-3(疏松土壤,对照)和1.55 g·cm-3(紧实土壤)的土壤进行盆栽试验,研究了土壤紧实胁迫对‘津春4号’黄瓜不同生育期根系生长、呼吸速率、活力及氮代谢的影响.结果表明:在土壤紧实胁迫条件下,黄瓜不同生育期根系总长度、表面积、分根数和根尖数均显著下降,根系的伸长生长及侧根的发生受到显著抑制,而根系的加粗生长得到激发,平均直径显著增加;根系活力和根系呼吸速率显著下降;根系中的NO3-、游离氨基酸和可溶性蛋白含量大幅下降,硝酸还原酶、谷氨酰胺合成酶和谷氨酸合酶活性显著降低,NH4+含量显著增加.说明在土壤紧实胁迫条件下黄瓜根系对硝态氮的吸收量减少,氨同化作用受到抑制,氮代谢显著受阻. 相似文献
15.
外源亚精胺对盐胁迫下黄瓜幼苗体内抗氧化酶活性的影响 总被引:10,自引:0,他引:10
以不同耐盐性黄瓜品种“长春密刺”和“津春2号”为材料,采用营养液栽培,研究了外源亚精胺(Spd)对NaCl胁迫下黄瓜幼苗叶片与根系中超氧阴离子(O2-.)产生速率、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的影响。结果表明,外源Spd对未经盐胁迫处理(对照)黄瓜幼苗体内O2-.产生速率、SOD、CAT和POD活性均无显著性影响;盐胁迫处理提高了O2-.产生速率,SOD、POD和CAT活性都有不同程度的升高;外源Spd处理进一步提高了盐胁迫下SOD、POD和CAT活性,减缓了O2-.产生速率。与耐盐型“长春密刺”品种相比,盐胁迫对盐敏感型“津春2号”影响较大,外源Spd对盐敏感型黄瓜品种盐胁迫伤害的缓解作用较大。表明盐胁迫下外源Spd可缓解盐胁迫对膜的伤害,从而提高黄瓜幼苗的耐盐性。 相似文献
16.
嫁接对铜胁迫下黄瓜幼苗根系多胺代谢的影响 总被引:4,自引:0,他引:4
采用营养液栽培法,研究了嫁接(以黑籽南瓜为砧木)对铜胁迫下黄瓜幼苗根系活力及多胺代谢的影响.结果表明:铜胁迫下黄瓜幼苗根系活力下降,电解质渗漏率升高,而嫁接苗的变化幅度显著小于黄瓜自根苗;铜胁迫下黄瓜嫁接植株根系中除游离态腐胺(Put)含量显著低于自根苗外,结合态和束缚态Put、3种形态亚精胺(Spd)和精胺(Spm)含量均显著高于自根苗,嫁接苗根系中游离态Put含量及腐胺/多胺(Put/PAs)显著低于自根苗;铜胁迫下,嫁接苗根系精氨酸脱羧酶(ADC)、鸟氨酸脱羧酶(ODC)和S-腺苷蛋氨酸脱羧酶(SAMDC)活性高于自根苗,而二胺氧化酶(DAO)和多胺氧化酶(PAO)活性显著低于自根苗.表明嫁接黄瓜幼苗根系PAs的合成增加,降解减少,使PAs含量维持在较高水平,从而提高了黄瓜幼苗抗铜胁迫能力. 相似文献
17.
Photosynthesis Research - When plants suffer from abiotic stresses, cyclic electron flow (CEF) is induced for photo-protection. Putrescine (Put), a primary polyamine in chloroplasts, plays a... 相似文献
18.
Comparative study of nitrogen fixation and carbon metabolism in two chick-pea (Cicer arietinum L.) cultivars under salt stress 总被引:1,自引:0,他引:1
Two cultivars of Cicer arietinum with differential
tolerance to salinity have been compared by analysing growth,
photosynthesis, nodulation, nitrogenase activity, and carbon metabolism in
the nodule cytosol. The aim was to help elucidate the relationships
between, on the one hand, sucrose and malate metabolism in nodules and, on
the other, the inhibition of nitrogen fixation under salt stress. Chick-pea
cultivars Pedrosillano (sensitive) and ILC1919 (tolerant) inoculated with
Mesorhizobium ciceri strain Ch-191 were grown in a
controlled environmental chamber and were treated with salt (0, 50, 75, and
100 mM NaCl) from sowing to harvest time (28 d). Plant growth and
photosynthesis were more affected by salt in Pedrosillano than in ILC1919.
Also the effect of salt on nodulation and nitrogen fixation was much more
pronounced in Pedrosillano. The increase in nodular mass in ILC1919 can
partially counteract the inhibition of nitrogenase activity. The enzymes of
sucrose breakdown were inhibited by NaCl, but in ILC1919 a rise in alkaline
invertase was observed with salinity, which could compensate for the lack
of the sucrose synthase hydrolytic activity. The activity of PEPC was
stimulated by salt in ILC1919. Also, this cultivar showed higher malate
concentrations in root nodules. 相似文献
19.
Effects of salt stress on basic processes of photosynthesis 总被引:2,自引:0,他引:2
Salt stress causes decrease in plant growth and productivity by disrupting physiological processes, especially photosynthesis. The accumulation of intracellular sodium ions at salt stress changes the ratio of K : Na, which seems to affect the bioenergetic processes of photosynthesis. Both multiple inhibitory effects of salt stress on photosynthesis and possible salt stress tolerance mechanisms in cyanobacteria and plants are reviewed. 相似文献
20.
Effects of salt stress on basic processes of photosynthesis 总被引:6,自引:0,他引:6
Salt stress causes decrease in plant growth and productivity by disrupting physiological processes, especially photosynthesis. The accumulation of intracellular sodium ions at salt stress changes the ratio of K : Na, which seems to affect the bioenergetic processes of photosynthesis. Both multiple inhibitory effects of salt stress on photosynthesis and possible salt stress tolerance mechanisms in cyanobacteria and plants are reviewed.This revised version was published online in March 2005 with corrections to the page numbers. 相似文献