Water structure changes induced by ceramics can be detected by increased permeability through aquaporin

Aquporins are intrinsic membrane proteins that function as water channel to transport water and/or mineral nutrients across biological membranes. In this study, we aimed to clarify whether water structure can be changed by the presence of ceramics and whether such a change can be determined by aquaporin. First, we confirmed that ceramics could transform tap water into active tap water by increasing water permeability through aquaporin. We also found that this change in water permeability by treatment with ceramics occurred in distilled water. The distilled water was determined to exhibit the same aquaporin permeability as the original tap water. Our data indicate that the aquaporin permeability of water can be changed by severe physical shocks, such as slapping and sonication, which is consistent with the implication that the aquaporin permeability is closely related to the structure of the water. In this study, using aquaporins, we first reported that the treatment of water with ceramics can affect the structure of water, and the water can retain the structure for a given period under certain condition     

The differential role of mechanisms for drought resistance in a Mediterranean evergreen shrub: a simulation approach

Plants have the ability to dampen the effects of variability in water resources. Various mechanisms contribute to these properties: reduction of leaf area, increased rooting depth and stomatal conductance. To evaluate the differential roles and interactions of these mechanisms, we have built a model and simulated flows of water in Mediterranean evergreen scrub. The essential concept of this model is that the water status of the canopy is governed by the water lost by transpiration, the availability of soil water and the hydraulic resistances to water flow in soil and plant. The amount of water supplied by the roots is related to changes in water potential between the soil and the leaf. The amount of water lost to the atmosphere is regulated by an interaction between atmospheric demand and canopy water potential. Water uptake by plant is assumed equal to plant water loss. Leaf area appears to affect largely the annual water balance. The critical leaf water potential required to reduce the maximum stomatal conductance by half has a dominant effect on annual leaf water potential. Reducing rooting depth induces a new functional equilibrium for the plant. This new equilibrium is reached by decreasing leaf area and the critical leaf water potential. Our results show the complexity of interactions of these mechanisms and highlight the importance of the coordination between them. Finally, we suggest a reconsideration of these mechanisms in a context of the survival and long-term persistence of the plant.     

Analysis of Water Sorption Isotherms of Superabsorbent Polymers by Solution Thermodynamics

Water sorption isotherms of superabsorbent polymers were measured, and their affinity for water was evaluated by solution thermodynamics. The results provide basic data for the functional packaging of food to control the water content of food during its transportation or storage. Water activity above 0.9 was measured by adding a specific amount of water to the samples, while that below 0.9 was measured with apparatus for evaluating water sorption isotherms. Thus, water sorption isotherms for superabsorbent polymers were obtained up to a water activity of approximately 0.98. The amount of water sorbed by the superabsorbent polymers was influenced by the type of functional groups in the polymers, and not by the degree of cross-linking in the polymers. The integral Gibbs free energy, which is the most suitable parameter for evaluating the affinity of a material for water, was evaluated from the water sorption isotherms by using solution thermodynamics.     

三江平原粮食作物生产水足迹时空特征及影响因素

水足迹是评价人类活动对水资源开采和水环境污染程度的重要方法,对农作物生长过程的绿水足迹、蓝水足迹和灰水足迹进行量化和分析,可以为农业用水综合评价和用水管理提供指导。以三江平原为研究区域量化粮食作物生产水足迹的时空特征,揭示粮食生产对区域水资源的占用情况,并分析水足迹的影响因素。结果表明:(1)三江平原粮食生产水足迹总量在2005-2018年间呈显著增加趋势,其中绿水足迹、蓝水足迹和灰水足迹在水足迹总量中的占比历年平均为28%、8%和64%;(2)粮食生产水足迹空间差异明显,在地市尺度,佳木斯市水足迹最高,占三江平原总量的47%,在县区尺度,富锦市、依兰县和桦南县是水足迹热点地区;(3)只考虑蓝水足迹,粮食生产给三江平原水资源造成轻度压力,而同时考虑蓝水和灰水足迹,粮食生产给三江平原水资源造成重度压力;各地市间水资源压力有较大差异,其中佳木斯市负担了该区域将近一半的粮食产量,水资源压力最高,鹤岗市则水资源压力最低;(4)降雨量、灌溉水利用效率、化肥施用量、粮食种植结构和作物单产水平等都会影响粮食作物生产水足迹,其中蓝水足迹响应种植结构的变化最敏感,灰水足迹响应化肥施用量的变化最敏感,而水足迹总量响应作物单产水平的变化最敏感。因此,建议减少化肥施用量、提高作物单产水平和优化作物种植结构纳入区域水资源可持续管理之中。     

内陆河干旱区生态需水分析

根据水分驱动生态演变模型 ,以水分运动和补给条件 ,研究了内陆河平原生态系统的需水结构。生态需水分为过渡带生态需水 ,绿洲生态需水 ;绿洲生态需水又进一步分为天然绿洲生态需水和人工绿洲生态需水。通过流域水分平衡和地面观测资料确定了生态圈层结构的水分需求 ,以及降水与径流对水分需求的补给比例。生态需水的分析计算 ,类似于供需平衡分析。从植物生理角度分析生态需水 ,得到天然植被的总腾发量 Et,作为植被生态需水总量。各典型天然植被的 Et通过实验资料获得。将植被和水面的总生态需水量扣除有效降水补充的部分 ,即为径流性生态需水量 GE。另一方面 ,以流域为单元进行降水和径流统一考虑的水分综合平衡 ,进行生态可利用水量分析。得到生态系统可能实际利用的径流性水资源量 GR。　　将生态需水量 GE与生态系统可能实际利用的水资源量 GR进行平衡分析 ,计算实际生态耗水 ,并分析盐碱地无效耗水。根据 2 0 2 0年生态状态情景分析 ,作出了 2 0 2 0年生态需水与生态系统结构演变的预测     

Soil Water Movement to Germinating Seeds

Water movement to germinating seeds of several range plantswas measured with a gammaray attenuation technique. Seeds wereplaced at a depth of 1.5 cm in soil at several water contentsand the soil water content of thin (3 mm) layers above and belowthe seeds was measured daily. It was found that the distancefrom which water was taken up by the seeds did not exceed 1cm and was not affected by soil water content. Germination wasfound to be dependent on seed water uptake, which was determinedby soil water content. The rate of seedling root growth wasnot affected by initial soil water content, but shoot growthwas strongly affected. Solutions of the water flow equation were applied to the caseof spheric geometry and infinite medium and to the case of linearflow and semi-infinite medium, in order to calculate water movementto a germinating seed or to a layer of seeds. Both the calculationsand the measurements showed that water uptake by a 2-mm-diameterseed from distances exceeding 1 cm was negligible.     

Electrical conduction in hydrated collagen. I. Conductivity mechanisms

The electrical conductivity of bovine Achilles tendon with various amounts of adsorbed water was measuredas a function of temperature. The conduction appeared to be fully determined by the water of hydration. The current is probably primarily carried by protons at water contents up to 45% and by small ions at water contents beyond 65%. In both ranges of water content, a linear relation between activation energy and water and content was found. As to the lower range, this is explained by the action of Coulombic forces during the separation of proton–hydroxyl ion pairs. In two regions of water content a linear relation between the logarithm of the pre-exponential factor and the activation energy was found. There are, however, indications that at certain water contents the dissociation constant of the adsorbed water is several orders of magnitude higher than in liquid water.     

Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status

The abiotic stresses of drought, salinity and freezing are linked by the fact that they all decrease the availability of water to plant cells. This decreased availability of water is quantified as a decrease in water potential. Plants resist low water potential and related stresses by modifying water uptake and loss to avoid low water potential, accumulating solutes and modifying the properties of cell walls to avoid the dehydration induced by low water potential and using protective proteins and mechanisms to tolerate reduced water content by preventing or repairing cell damage. Salt stress also alters plant ion homeostasis, and under many conditions this may be the predominant factor affecting plant performance. Our emphasis is on experiments that quantify resistance to realistic and reproducible low water potential (drought), salt and freezing stresses while being suitable for genetic studies where a large number of lines must be analyzed. Detailed protocols for the use of polyethylene glycol-infused agar plates to impose low water potential stress, assay of salt tolerance based on root elongation, quantification of freezing tolerance and the use of electrolyte leakage experiments to quantify cellular damage induced by freezing and low water potential are also presented.     

Isotopic assessment of fog drip water contribution to vegetation during dry season in Junshan wetland,northern Dongting Lake

Stable isotopes ²H and ¹⁸O of fog drip water, lake water, soil profiles, and vegetation leaves in Junshan wetland of East Dongting Lake, China, were investigated to estimate the contribution of fog drip water to wetland vegetation. Because of its recycled terrestrial meteoric water source, fog drip water is characterized by isotopic compositions that plot above the local meteoric water line (LMWL). Lake water undergoes intense evaporation owing to its low water volume and long residence time. The isotopic compositions of soil water suggest that it is recharged by both recent rainfall and fog drip water. A binary mixing model shows that approximately 16% of unsaturated soil water originates from continuous recharge by fog drip water, reaching 31% in surface soil where the vegetation roots are mainly distributed. Inspired by the literatures on acidification of intercepted clouds and fog as the major factor in forest die-back in Europe, the findings in this study inform future investigations into the relationships between fog water deposition and wetland degradation especially in heavily industrialized foggy areas.     

Go with the flow: mechanisms driving water transport during vegetative growth and fruiting

Fungi need water for all stages of life. Notably, mushrooms consist of ∼90% water. Fungi degrade organic matter by secreting enzymes. These enzymes need water to be able to break down the substrate. For instance, when the substrate is too dry, fungi transport water from moist areas to arid areas by hydraulic redistribution. Once nutrients are freed from the substrate, they are taken up by transporters lining the cell membrane. Thereby an intracellular osmotic potential is created which is greater than that of the substrate, and water follows by osmosis. Aquaporins may facilitate water uptake depending on the conditions. Since fungi possess a cell wall, the cell volume will not increase much by water uptake, but the cell membrane will exert higher pressure on the cell wall, thereby building up turgor. Fungi have tightly coordinated osmotic regulatory controls via the HOG pathway. When water is getting scarce, this pathway makes sure that enough osmolytes are synthesized to allow sufficient water uptake for maintaining turgor homeostasis. The fungal network is interconnected and allows water flow when small pressure differences exist. These pressure differences can be the result of growth, differential osmolyte uptake/synthesis or external osmotic conditions. Overall, the water potential of the substrate and of fungal tissues determine whether water will flow, since water flows from an area of high- to a low water potential area, when unobstructed. In this review we aim to give a comprehensive view on how fungi obtain and translocate water needed for their development. We have taken Agaricus bisporus growing on compost and casing soil as a case study, to discuss water relations during fruiting in detail. Using the current state-of-the-art we found that there is a discrepancy between the models describing water transport to mushrooms and the story that water potentials tell us.     

Cholinergic and adrenergic effects on diffusional water flux in the toadfish, Opsanus beta

Intraperitoneal injections of adrenaline resulted in increased tritiated water efflux rate in the toadfish, Opsanus beta. Adrenaline-stimulated water flux was inhibited by the beta-adrenergic blocker, propranolol, but not by the alpha-adrenergic blocker, phentolamine. Propranolol on its own had no effect but phentolamine significantly stimulated water flux; this action was attributed to a beta-mimetic effect of the drug. The cholinergic neurotransmitter acetylcholine, had no effect while the parasympathico-mimetic carbachol, significantly stimulated water flux. Arguments were advanced to explain the similarity in the effects of the adrenergic and cholinergic drugs although they are both known to produce opposing vascular haemodynamic effects in fish gills. Adrenaline substantially stimulated tritiated water flux in the toadfish, Opsanus beta. The adrenaline-stimulated water flux exhibited a linear dose-response curve up to an adrenaline dosage of 750 micrograms kg-1; wt. At higher doses there was apparently a desensitization of the beta-adrenergic receptor sites. The adrenaline effect was inhibited by the beta-blocker propranolol, but not by the alpha-blocker, phentolamine. This suggests that the adrenaline-stimulated water flux was due predominantly to beta-receptor site stimulation. Stimulation of water flux by phentolamine on its own could be due to the stimulation of endogenous catecholamine release by the drug. We have proposed that the beta-stimulated water efflux could be due to an increase in surface area of the branchial epithelium, a decrease in water to blood diffusion distance, a direct metabolic effect or any combination of these effects by adrenaline. Carbachol caused an increase in tritiated water efflux. The carbachol-stimulated water flux was inhibited by atropine thus suggesting that the drug acts via muscarinic receptor sites. We have suggested that the action of the drug on hydraulic water conductivity, water to blood diffusion distance, hydrostatic pressure or a direct effect on membrane diffusion coefficient.     

Spatial segregation between the water opossum Chironectes minimus and the water rat Nectomys squamipes: Just competition avoidance or a conservation problem as well?

Riparian zones, as spatially constrained environments, can favor intense competition among similar species and thus the occurrence of spatial segregation. We investigated the spatial pattern of water opossum Chironectes minimus and water rat Nectomys squamipes captures in Atlantic Forest rivers in southeast Brazil. Our goals were to test if the water opossum and water rat are spatially segregated, to describe the habitat selection by these two species and to verify if habitat degradation favors the water rat, at the expense of water opossum. The abundances of water opossum and water rat were negatively correlated. Water opossum abundance was higher in wide rivers with high tree density in the riverbank, while water rat abundance was higher in narrow streams within non-forested areas. The ratio between the water opossum and water rat relative abundances was positively related to the degree of conservation of the riverine habitat. The segregation showed by the water opossum and the water rat was produced by their morphological and ecological differences, rather than by interespecific competition. Based on ours results we predict that deforestation, especially the removal of riparian vegetation, will result in a reduction of the suitable habitat for the water opossum while the water rat will expand its range.     

Effect of distension on ADH-induced osmotic water flow in toad urinary bladder

Summary We recently described a method by which the resistance to water flow of the luminal membrane of ADH-stimulated toad bladder can be quantitatively distinguished from that of barriers lying in series with it. This method requires estimates of both total bladder water permeability (assessed by transbladder osmotic water flow at constant gradient) and luminal membrane water permeability (assessed by quantitation of the frequency of ADH-induced luminal membrane particle aggregates). In the present study we examined the effect of bladder distension on transepithelial osmotic water flow before and during maximal ADH stimulation. Base-line water flow was unaffected by bladder distension, but hormonally stimulated flow increased systematically as bladders became more distended. Distension had no effect on the frequency of ADH-induced intramembranous particle aggregates. By comparing the relationships between aggregate frequency and hormonally induced water permeability in distended and undistended bladders, we found that distension appeared to enhance ADH-stimulated water flow by decreasing the resistance of the series permeability barrier while the apparent water permeability associated with each single luminal membrane aggregate was unaffected. In that bladder distension causes tissue thinning, the series resistance limiting ADH-stimulated water flow appears to be accounted for by deformable barriers within the bladder tissue itself, probably unstirred layers of water.     

Drought-induced root aerenchyma formation restricts water uptake in rice seedlings supplied with nitrate

Previous studies demonstrated that ammonium nutrition results in higher water uptake rate than does nitrate nutrition under water stress, and thus enhances the tolerance of rice plants to water stress. However, the process by which water uptake is related to nitrogen form under water stress remains unknown. A hydroponic experiment with simulated water stress induced by polyethylene glycol (PEG6000) was conducted in a greenhouse to study the relationship between root aerenchyma formation and water uptake rate, such as xylem sap flow rate and hydraulic conductance, in two different rice cultivars (cv. 'Shanyou 63' hybrid indica and cv. 'Yangdao 6' indica, China). The results showed that root aerenchyma tissue increased in water-stressed plants of both cultivars fed by nitrate. No significant difference was found in root hydraulic conductivity and/or xylem sap flow rate between the two rice cultivars fed by ammonium regardless of water status, whereas these parameters decreased significantly in water-stressed plants fed by nitrate. It was concluded that aerenchyma that formed in the root cortex impeded the radial transport of water in the root cylinder and decreased water uptake in water-stressed rice plants fed by nitrate. Water transport occurred mainly through Hg-sensitive water channels in rice roots supplied with ammonium.     

Glass transition and dynamics in BSA-water mixtures over wide ranges of composition studied by thermal and dielectric techniques

Protein-water dynamics in mixtures of water and a globular protein, bovine serum albumin (BSA), was studied over wide ranges of composition, in the form of solutions or hydrated solid pellets, by differential scanning calorimetry (DSC), thermally stimulated depolarization current technique (TSDC) and dielectric relaxation spectroscopy (DRS). Additionally, water equilibrium sorption isotherm (ESI) measurements were performed at room temperature. The crystallization and melting events were studied by DSC and the amount of uncrystallized water was calculated by the enthalpy of melting during heating. The glass transition of the system was detected by DSC for water contents higher than the critical water content corresponding to the formation of the first sorption layer of water molecules directly bound to primary hydration sites, namely 0.073 (grams of water per grams of dry protein), estimated by ESI. A strong plasticization of the T(g) was observed by DSC for hydration levels lower than those necessary for crystallization of water during cooling, i.e. lower than about 0.3 (grams of water per grams of hydrated protein) followed by a stabilization of T(g) at about -80°C for higher water contents. The α relaxation associated with the glass transition was also observed in dielectric measurements. In TSDC a microphase separation could be detected resulting in double T(g) for some hydration levels. A dielectric relaxation of small polar groups of the protein plasticized by water, overlapped by relaxations of uncrystallized water molecules, and a separate relaxation of water in the crystallized water phase (bulk ice crystals) were also recorded.     

Analysis of cellular water content in T cells reveals a switch from slow metabolic water gain to rapid water influx prior to cell division

Cell growth is driven by the acquisition and synthesis of both dry biomass and water mass. In this study, we examine the increase of water mass in T cell during cell growth. We found that T-cell growth is characterized by an initial phase of slow increase in cellular water, followed by a second phase of rapid increase in water content. To study the origin of the water gain, we developed a novel methodology we call cold aqua trap-isotope ratio mass spectrometry, which allows analysis of the isotope composition of intracellular water. Applying cold aqua trap-isotope ratio mass spectrometry, we discovered that glycolysis-coupled metabolism of water accounts on average for 11 fl out of the 20 fl of water gained per cell during the initial slow phase. In addition, we show that at the end of the rapid phase before initiation of cell division, a water influx occurs, increasing the cellular water mass by threefold. Thus, we conclude that activated T cells switch from metabolizing water to rapidly taking up water from the extracellular medium prior to cell division. Our work provides a method to analyze cell water content as well as insights into the ways cells regulate their water mass.     

Measurement of energy expenditure in humans by doubly labeled water method

The utility of the doubly labeled water method for the determination of energy expenditure and water output was investigated in humans. Approximately 10 g of 18O and 0.5 g of 2H as water was orally administered to four healthy adults. Total body water was determined from the isotope dilution, and the ensuing 18O and 2H disappearance rates from body water were determined for 13 days by mass spectrometric isotope ratio analysis of the urinary water. During this period, subjects were maintained on a measured diet to determine energy and water intake. The energy expenditure from the doubly labeled water method differed from dietary intake plus change in body composition by an average of 2%, with a coefficient of variation of 6%. The water outputs determined by the two methods differed by 1%, with a coefficient of variation of 7%. The doubly labeled water method is noninvasive, and the subjects could maintain their daily activities without restriction.     

Water resources stress assessment and risk early warning–a case of Hebei Province China

In most parts of China, water resources development and utilization has reached or exceeded the international warning level, especially in the northern arid and semi-arid regions, the serious water stress have aroused worldwide concerns. Many issues of water resources are closely and some inseparably associated with human activities. In this study, the water footprint (WF) and related indicators were applied as comprehensive indicators to assess real water use by humans from the consumption perspective. A Fuzzy Comprehensive Evaluation Model (FCE) was used for the evaluation in its role to assess water stress. And the index system of water stress assessment including four aspects, namely water supply and demand balance, socio-economy, agricultural production and ecological environment was established. Taking the case of Hebei province of China where there is serious conflict between water supply and demand, water stress assessment of all eleven administrative areas for the period 2000–2013 was analyzed. The findings showed that water stress was denoted by three levels, namely high water stress (red zone), general water stress (yellow zone) and low water stress (blue zone), which lays the foundation of the further research on water risk warning. According to the results of water stress assessment and the socio-economic development trends of Hebei, a Markov Chain Risk Warning model was built to describe the risk state of water system and to predict the transition probability of future states. This was intended to focus on the possibility that water stress levels might change into higher water stress level. The current study aims at extending earlier research by making a first step from water footprint estimation towards water stress assessment and providing reference for scientific management of water resources in arid and semi-arid regions.     

Effect of vanadate on water transport by the toad bladder

Vanadate increases renal Na and water excretion. The mechanism whereby vanadate impairs water transport was examined in the toad bladder. Vanadate did not alter baseline water transport but caused a significant inhibition of water transport elicited by high doses of AVP. The inhibition of AVP stimulated water flow by vanadate was dose dependent with inhibition present with concentration as low as 10(-7) and maximal inhibition occurring at 10(-5) M. Vanadate also inhibited water transport stimulated by cyclic AMP or by phosphodiesterase inhibition indicating that vanadate has an effect beyond cyclic AMP step, in addition to whatever effect it might have on adenylate cyclase. The inhibitory effect of vanadate on AVP stimulated water flow was not altered by prior Na-K-ATPase or prostaglandin inhibition. Since vanadate has been shown to stimulate adenylate cyclase in other tissues we examined whether addition of vanadate 10 minutes after addition of AVP would enhance water transport. Vanadate caused a transient enhancement of AVP stimulated water flow. These data demonstrate that vanadate can inhibit or stimulate water flow in the toad bladder.     

Notes on water-contact by a captive male lowland gorilla

Great apes in captivity and in the wild exhibit varying propensities to enter and use water. Although some captive apes have drowned in deep water, others have been inclined to play and to “bathe” in shallow water. In the present study of two gorillas, an adult male's water contact was apparently influenced by humidity, but not by zoo attendance or ambient temperature. Most water contact was restricted to the morning hours; water entry during the afternoon was infrequent. Both animals sought shelter during rain. Considering the frequent use of the streams and moat by one of the two gorillas studied, water may be regarded as a type of environmental enrichment.