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1.
Indole-3-acetic acid (IAA) and gibberellic acid (GA3) are essential for the growth and development of plants. In the present study, the ameliorative potential of these phytohormones on growth, protein content, and antioxidant enzymes was investigated in in vitro-grown Solanum tuberosum L. cultivars ‘Cardinal’ and ‘Desiree’ under salt stress. A 4 × 3 factorial combination of 0, 40, 60, or 80 mM NaCl with 0, 7, or 14 μM IAA, or 0, 14, or 21 μM GA3, were added to Murashige and Skoog (MS) basal medium, followed by inoculation of nodal explants or callus cultures. The data for root and shoot number and length, number of nodes and leaves, fresh weight of plants, increase or decrease in fresh weight of callus cultures, total soluble protein, and superoxide dismutase (SOD) and peroxidase (POD) activities were recorded after 30 d. The growth of both callus cultures and nodal explants subjected to NaCl stress was substantially reduced compared with the control. Both IAA and GA3 successfully alleviated the harmful effects of salt stress on all of the growth parameters studied. Salt stress resulted in decreased protein content, which increased when the media also contained phytohormones. The activities of SOD and POD were increased with either IAA or GA3 under NaCl stress. Therefore, the exogenous application of both IAA and GA3 not only played a positive role in terms of in vitro potato growth but also significantly affected the biochemical parameters tested. 相似文献
2.
The fern Asplenium nidus L. is in great demand as an ornamental plant. The aim of this work was to investigate the influence of phytohormones in promoting
a gametophytic and sporophytic growth in homogenized sporophytes tissue. Exogenous application of 0.5 and 5 μM N
6-benzyladenine, 0.05 and 0.5 μM indole-3-acetic acid (IAA), and 0.3 and 3 μM gibberellic acid (GA 3) favoured sporophyte regeneration, whereas gametophyte regeneration took place when plant material was cultured in a hormone-free
liquid MS medium. The endogenous contents of the auxin IAA, the cytokinins trans-zeatin, trans-zeatin riboside, dihydrozeatin, dihydrozeatin riboside, isopentenyladenine and isopentenyladenosine, and the gibberellins
GA 1, GA 3, GA 4, GA 7, GA 9 and GA 20 in growing gametophytes and sporophytes were evaluated. Similar levels of the auxin and cytokinins and qualitative differences
in the gibberellins were found between both generations. 相似文献
3.
以云南省瑞丽市勐秀林场扦插种植的薇甘菊为试材,采用液相色谱串联质谱(LC-MS/MS)技术对花芽未分化期和花序原基分化期花芽中的生长素(IAA)、赤霉素(GA)、脱落酸(ABA)、反式玉米素(tZ)、异戊烯腺嘌呤(IP)、1-氨基环丙烷羧酸(ACC)、茉莉酸(JA)和水杨酸(SA)含量进行定量分析,同时基于转录组基因功能注释数据对内源激素合成、代谢及信号转导途径相关基因进行表达分析,以探讨不同内源激素对薇甘菊花芽形成的调控作用,以及内源激素合成和信号转导途径相关基因调控薇甘菊花芽分化的机制,为后期通过外源激素调控薇甘菊内源激素水平的方式来控制薇甘菊的有性繁殖提供理论和技术支持。结果表明:(1)薇甘菊未分化期花芽中GA 15、GA 19、GA 20、GA 24、IAA、ABA和ETH含量低于花序原基分化期,而未分化期花芽中两种细胞分裂素tZ和IP含量显著高于花序原基分化期。(2)基于RNA-seq测序结果,在薇甘菊两个花芽分化时期共获得7 116个差异表达基因(DEGs),其中上调3 907个,下调3 209个。(3)在内源激素合成方面,参与GA 15、GA 19、GA 20、GA 24、IAA、ABA和ACC合成的大量DEGs在花序原基分化期上调表达,这与它们在薇甘菊花序原基分化期的高含量趋势相一致;参与IAA合成的 YUCCA基因家族和ACC合成的 ACS基因在花序原基分化期的高表达也可能参与促进薇甘菊花芽分化。(4)在植物激素转导途径中,在花序原基分化期,生长素信号转导途径通过 AUX/IAA(gene-E3N88_07743)的下调表达和 ARF(gene-E3N88_41119)的上调表达,乙烯信号转导途径通过 ERF(gene-E3N88_41547)的上调表达,赤霉素信号转导途径通过 GID1(gene-E3N88_19448)基因的上调表达,细胞分裂素信号转导途径通过 B-ARR(gene-E3N88_28086)和 A-RRR(gene-E3N88_40764)基因的下调表达,脱落酸途径通过 AREB(gene-E3N88_18558)基因的上调表达,茉莉酸信号转导途径通过 JAZ(gene-E3N88_05628)的上调表达和 MYC2(gene-E3N88_32405)的下调表达来调控薇甘菊花芽分化。研究发现,高水平的GA 15、GA 19、GA 20、GA 24、IAA、ABA和ACC有利于薇甘菊的花芽分化;薇甘菊在花芽分化过程中通过改变不同种类内源激素合成、代谢基因的表达来调控激素浓度,而激素又通过信号转导途径引起下游基因的表达,进而调控薇甘菊的花芽分化。 相似文献
4.
A number of studies have used microspore-derived embryos (MDEs) as amodel for examining a range of processes, including hormonal regulation ofembryo development. We examined the hormonal physiology of MDEs with theprimaryobjective of testing the validity of using the MDE system as a model forhormonally-regulated development in zygotic embryos, through late stages. To dothis we identified and quantified endogenous levels of abscisic acid (ABA),indole-3-acetic acid (IAA) and a number of gibberellins (GAs), includingGA 19, GA 20, GA 1 and GA 8 in bothMDEsand zygotic embryos. The presence of GA 19, together with itsC 19 metabolites indicates that the early-13 hydroxylation pathway isoperative in both embryo systems. Gibberellins A 4 and GA 9were also identified, thereby confirming the presence of the earlynon-hydroxylation pathway in B. napus MDEs and zygoticembryos. In general, the pattern of change of hormone (ABA, IAA, GA 1and GA 20) content per embryo through embryogenesis was similar forMDEs and zygotic embryos. Indole-3-acetic acid and GA 1 increased toamaximum at day 30 after culture (DAC) before decreasing. Abscisicacid levels increased to a maximum at day 35, and declined in zygoticembryos but not in MDEs. GA 20 increased to the final harvest atmaturity, or day 40. The absolute content (g/embryo) of each hormone, howeverwas appreciably lower (5- to 15- fold) in the MDEs. This was not the result ofdilution into surrounding medium for ABA or IAA; GA 1, however, didaccumulate in the medium. Although there were absolute quantitative differencesin the levels of IAA and ABA found in the two embryo systems, the similaritiesin the pattern of hormone changes suggests that the MDE system can serve as auseful model for examining the physiological roles of hormones duringembryogenesis. 相似文献
5.
Uninuclear microspores in red horse chestnut anther cultures formed pollen embryos and plantlents in MS agar medium supplemented with varying 2,4-D concentrations (1.0, 1.5 or 2.0 mg l -1) and 1.0 mg l -1 Kin. The highest number of embryogenic anthers (38%) was obtained in MS medium containing 1.0 mg l -1 of each 2,4-D and Kin. The ability of pollen embryos to germinate was closely correlated with normal embryo morphology and was influenced by hormone content in the medium (MS+;1.0 mg l -1 IAA+1.0 mg l -1 GA 3+0.1 mg l -1 Kin+400 mg l -1 glutamine). Pollen embryos and plantlets had the haploid chromosome number ( x=n=40). Cytological examinations demonstrated pollen dimorphism of this Aesculus species.Abbreviations AC
activated charcoal
- 2,4-D
2,4-dichlorophenoxyacetic acid
- IAA
indole-3-acetic acid
- Kin
6-furfurylaminopurine
- GA 3
gibberellic acid
- MS
Murashige and Skoog 相似文献
6.
Preussia sp. have been least known to improve plant growth and produce phytohormones. The current study investigated the production of nitric oxide (NO), indole-3-acetic acid (IAA), and gibberellins (GA 4, GA 7, GA 15, and GA 53) by a novel endophytic-fungal strain Preussia sp . BSL-10 using advanced chromatographic and spectroscopic techniques. Production of these phytohormones were validated by RT–PCR analysis, which indicated the expression of genes encoding tryptophan synthase ( TRP), indole-3-acetamide hydrolase (IAAH), tryptophan-2-monooxygenase (IAAM), aldehyde dehydrogenase (ALD), GA4 desaturase (DES), geranylgeranyl-diphosphate synthase (GGS2), ent-desaturase oxidase (P450-4), GA14 synthase (P450-1) and nitrite reductase (NIRK/NIRS), cytochrome P450 (P450nor), nitrate reductase (NR), NOS-like (NOL), and nitric oxide reductase (QNOR/CNOR). In plant growth-promoting effects, the inoculation of Preussia sp. BSL-10 significantly increased the growth of dwarf mutant Waito-C and wild-type rice cultivars. In conclusion, utilizing new endophytic with the ability to produce NO, IAA, and gibberellins can be used to promote growth and yield of marginalized crops. 相似文献
7.
Sunflower hypocotyls elongate as light quality changes from the normal red to far-red (R/FR) ratio of sunlight to a lower
R/FR ratio. This low R/FR ratio-induced elongation significantly increases endogenous concentrations of indole-3-acetic acid
(IAA) and also of three gibberellins (GAs): GA 20, GA 1, and GA 8. Of these, it is likely GA 1 that drives low R/FR-induced growth. Brassinosteroids are also involved in shoot growth. Here we tested three R/FR ratios:
high, normal, and low. Significant hypocotyl elongation occurred with this stepwise reduction in R/FR ratio, but endogenous
castasterone concentrations in the hypocotyls remained unchanged. Brassinolide was also applied to the seedlings and significantly
increased hypocotyl growth, though one that was uniform across all three R/FR ratios. Applied brassinolide increased hypocotyl
elongation while significantly reducing (usually) levels of IAA, GA 20, and GA 8, but not that of GA 1, which remained constant. Given the above, we conclude that endogenous castasterone does not mediate the hypocotyl growth
that is induced by enriching FR light, relative to R light. Similarly, we conclude that the hypocotyl growth that is induced
by applied brassinolide does not result from an interaction of brassinolide with changes in light quality. The ability of
applied brassinolide to influence IAA, GA 20, and GA 8 content, yet have no significant effect on GA 1, is hard to explain. One speculative hypothesis, though, could involve the brassinolide-induced reductions that occurred
for endogenous IAA, given IAA’s known ability to differentially influence the expression levels of GA20ox, GA3ox, and GA2ox, key genes in GA biosynthesis. 相似文献
8.
The effects of the auxin transport inhibitors 2,3,5-triiodobenzoic acid (TIBA), 9-hydroxyfluorene-9-carboxylic acid (HFCA),
and 1- N-naphthylphthalamic acid (NPA) on gibberellins (GAs) in the garden pea ( Pisum sativum L.) were studied. Application of these compounds to elongating internodes of intact wild type plants reduced markedly the
endogenous level of the bioactive gibberellin A 1 (GA 1) below the application site. Indole-3-acetic acid (IAA) levels were also reduced, as was internode elongation. The auxin
transport inhibitors did not affect the level of endogenous GA 1 above the application site markedly, nor that of GA 1 precursors above or below it. When plants were treated with [ 13C, 3H]GA 20, TIBA reduced dramatically the level of [ 13C, 3H]GA 1 recovered below the TIBA application site. The internodes treated with auxin transport inhibitors appeared to be still in
the phase where endogenous GA 1 affects elongation, as indicated by the strong response to applied GA 1 by internodes of a GA 1-deficient line at the same stage of expansion. On the basis of the present results it is suggested that caution be exercised
when attributing the developmental effects of auxin transport inhibitors to changes in IAA level alone.
Received April 13, 1998; accepted April 14, 1998 相似文献
9.
Prohexadione, a gibberellin (GA) biosynthesis inhibitor, was applied in ethanol around the circumference at the midpoint of the previous year terminal shoot of dormant Pinus sylvestris seedlings. After cultivating the seedlings under environmental conditions favorable for growth for 10 weeks, longitudinal and cambial growth were measured, and the endogenous levels of GA 1, GA 3, GA 4, GA 9, and indole-3-acetic acid (IAA) were determined by combined gas chromatography-mass spectrometry, using deuterated GAs and [ 13C 6]IAA as internal standards. Prohexadione application inhibited elongation and xylem and phloem production in the current year terminal shoot and xylem production in the previous year terminal shoots. Concomitantly, in both ages of shoots the cambial region contents of GA 1; GA 3, and GA 4 were decreased, whereas the level of GA 9 was increased. However, the IAA content was not altered in the terminal bud on the current year terminal shoot or in the cambial region of the current year or previous year terminal shoots. The results provide additional evidence that: (1) GAs are involved in the regulation of cambial growth, as well as longitudinal growth, in Pinus sylvestris shoots; (2) they act directly, rather than indirectly, by altering the IAA level; and (3) the GA 9 GA 4 GA 1 pathway is a major route of GA biosynthesis in conifer species.Abbreviations GA
gibberellin
- IAA
indole-3-acetic acid
- HPLC
high performance liquid chromatography
- GC
gas chromatography
- SIM
selected ion monitoring
- MS
mass spectrometry 相似文献
10.
为探明华重楼离体胚培养及植株再生的基本体系,该文以华重楼离体幼胚为试验材料,以MS培养基为基本培养基,研究不同光照、不同浓度梯度组合的植物生长调节剂对华重楼离体幼胚萌发、成苗的影响。结果表明:培养到60 d时,暗培养条件下华重楼离体幼胚的生长率和萌发率分别比光培养条件下高45.25%、19.17%,故暗培养比光培养更有利于华重楼离体幼胚生长发育。当GA 3浓度相同时,离体幼胚萌发所需时间随IAA浓度增加而延长。不同浓度的GA 3都可促进离体幼胚萌发,促进作用由强到弱依次为5 mg·L -1GA 3>1 mg·L -1GA 3> 10 mg·L -1GA 3。在黑暗条件下,优选得到最适华重楼离体幼胚生长发育配方为1/2 MS+30g·L -1蔗糖+7 g·L -1琼脂+0.5 g·L -1活性炭+5 mg·L -1GA 3 相似文献
11.
This work showed for the first time the relationship between the effect of exogenous auxins and gibberellins on apogamy in
Dryopteris affinis (Lowe) Fraser-Jenkins sp. affinis and its endogenous contents during early apogamic events. The addition of NAA (0.53 and 5.37 μM) or GA 3 2.8 μM to an MS solid medium significantly increased apogamous sporophyte formation. BA induced brown callus that regenerated
sporophytes in a hormone-free medium. The endogenous contents of GA 1, GA 3, GA 4, GA 7, GA 9 and IAA were determined by GC–MS in gametophytes cultured on MS solid medium, before and during early stages of apogamous
embryo development. The accumulation of both GA 9 and IAA before embryo development was evident as high levels of GA 4 in the earliest analysed stage of embryo development and high levels of GA 3 in elongating shoots were found. The role of gibberellins on apogamy was also supported by data showing a decrease in the
percentage of gametophytes developing embryos because of the addition of flurprimidol to the culture medium. 相似文献
12.
Relationships between sex‐specific floral traits and endogenous phytohormones associated with altitude are unknown particularly in dioecious trees. We thus examined the relationships between floral morphology or biomass and phytohormones in male and female flowers of dioecious Populus cathayana populations along an altitudinal gradient (1,500, 1,600, and 1,700 m above sea level) in the Xiaowutai Nature Reserve in northern China. The female and male flowers had the most stigma and pollen at 1,700 m, the largest ovaries and least pollen at 1,500 m, and the smallest ovaries and greater numbers of anthers at 1,600 m altitude. The single‐flower biomass was significantly greater in males than in females at 1,600 or 1,700 m, but the opposite was true at 1,500 m altitude. The biomass percentages were significantly higher in anthers than in stigmas at each altitude, while significantly greater gibberellin A3 (GA 3), zeatin riboside (ZR), indoleacetic acid (IAA), and abscisic acid (ABA) concentrations were found in female than in male flowers. Moreover, most flower morphological traits positively correlated with IAA in females but not in males. The biomass of a single flower was significantly positively correlated with ABA or IAA in males but negatively with ZR in females and was not correlated with GA 3 in both females and males. Our results demonstrate a distinct sexual adaptation between male and female flowers and that phytohormones are closely related to the size, shape, and biomass allocation in the pollination or fertilization organs of dioecious plants, although with variations in altitude. 相似文献
13.
Summary Haploids from Hordeum vulgare (2n = 14) X H. Bulbosum (2n = 14) crosses result after fertilization from the subsequent elimination of bulbosum chromosomes during early embryo development. Seed set from the cross is high but embryo culture is necessary to obtain seedlings. Application of gibberellin A 3 (GA 3) to pollinated florets was effective for increasing the frequency of haploid seedlings produced on both nutrient-fed detached tillers and intact plants. GA 3 increased both seed set and embryo yield. The number of cells per embryo during its transition to the haploid state was increased two to three times following GA 3 treatments. Enhanced embryo and endosperm development was attributed to increased mitotic activity. The number of visibly differentiated embryos was doubled to about 35 % of the cultured embryos after GA 3 was applied to detached tillers in nutrient solution. About 70 % of the resulting haploid plants developed from the visibly differentiated embryos. The detached tiller technique offers a convenient method of culturing haploids from field-grown plants. 相似文献
14.
In vitro culture of Chenopodium murale L. (ecotype 197) green and herbicide SAN 9789 - treated "white" plants was established and the effects of benzylaminopurine (BAP), indole-3-acetic acid (IAA) and gibberellic acid (GA 3) on growth and flowering were tested. Green plants did not flower on glucose free media, while 17 % of plants flowered on 5 % glucose-containing medium. SAN 9789 (10 –5 M) inhibited growth and flowering. BAP and IAA (0.1 – 5 mg dm –3) also inhibited growth and flowering of green and "white" plants. GA 3 (10 mg dm –3) stimulated leaf development in green plants, but had no significant effect on "white" plants, and stimulated flowering of green (41 %) and "white" (33 %) plants. 相似文献
15.
Changes in agronomic characters and the profile of various endogenous phytohormones during tuber development were studied in Dioscorea opposite (Chinese yam) cv. Guihuai 16. Tuber development exhibited a sigmoidal growth pattern according to the changes in tuber agronomic characters. The growth cycle of yam tuber could be divided into three stages: initiation stage, enlargement stage, and maturation stage. Moreover, the enlargement stage could be separated into three phases—slow growth phase, rapid growth phase, and late growth phase. Endogenous changes in phytohormones were associated with developmental changes in the tubers. The pulses of bioactive gibberellins (such as GA 3 and GA 4) were measured in tubers. The highest contents of GA 3 and GA 4 were reached 90 days after field planting, corresponding to the beginning of the rapid growth phase of tuber enlargement. Changes in trans-zeatin ( tZ), jasmonic acid (JA), indole-3-acetic acid (IAA), and abscisic acid (ABA) levels were also observed, and seemed to be related to tuber enlargement at different phases. Continuous increases in JA and tZ contents accompanied tuber enlargement. Transient pulses of both IAA and ABA contents were also observed at the start of tuber rapid growth. Additionally, a second peak level of IAA was detected at the tuber maturation stage. These results suggest GAs play a key role at the beginning of the tuber rapid growth stage, and there is a close relationship between whole tuber enlargement and the contents of JA and tZ. Moreover, it is suggested that IAA and ABA also may be linked to the beginning of tuber rapid growth, and IAA also seems to be correlated to late tuber maturation. 相似文献
16.
Changes in gibberellins (GAs), indole-3-acetic acid (IAA), and cytokinins associated with the transition from vegetative growth
to reproductive growth in Humulus lupulus L. buds and leaves harvested at fortnight intervals were studied. During vegetative growth, GA 1 increased gradually and the lowest content was observed during flower development. Both GA 3 and GA 4 showed a dramatic increase in the samples taken from the apical part of axillary branches from plants 4–5 m high, which corresponds
to the maximum vegetative development prior to macroscopically visible inflorescences. Notable increases in the cytokinins
trans-zeatin ( t-Z), isopentenyladenine (iP), and the riboside and ribotide forms of iP were also obtained. The auxin, indole-3-acetic acid,
was the most abundant plant hormone, and its content was highest during vegetative growth. These results show for the first
time a relationship between endogenous hormone profiles and both vegetative and reproductive development in hop plants, which
may be relevant for future research on the control of the flowering by exogenous hormone applications. 相似文献
17.
Soybean is an important oilseed crop grown globally. However, two examples of environmental stresses that drastically regulate soybean growth are low light and high-temperature. Emerging evidence suggests a possible interconnection between these two environmental stimuli. Low light and high-temperature as individual factors have been reported to regulate plant hypocotyl elongation. However, their interactive signal effect on soybean growth and development remains largely unclear. Here, we report that gibberellins (GAs) and auxin are required for soybean hypocotyl elongation under low light and high-temperature interaction. Our analysis indicated that low light and high-temperature interaction enhanced the regulation of soybean hypocotyl elongation and that the endogenous GA 3, GA 7, indole-3-acetic acid (IAA), and indole-3-pyruvate (IPA) contents significantly increased. Again, analysis of the effect of exogenous phytohormones and biosynthesis inhibitors treatments showed that exogenous GA, IAA, and paclobutrazol (PAC), 2, 3, 5,-triiodobenzoic acid (TIBA) treatments significantly regulated soybean seedlings growth under low light and high-temperature interaction. Further qRT-PCR analysis showed that the expression level of GA biosynthesis pathway genes ( GmGA3ox1, GmGA3ox2 and GmGA3) and auxin biosynthesis pathway genes ( GmYUCCA3, GmYUCCA5 and GmYUCCA7) significantly increased under (i) low light and high-temperature interaction and (ii) exogenous GA and IAA treatments. Altogether, these observations support the hypothesis that gibberellins and auxin regulate soybean hypocotyl elongation under low light and high-temperature stress interaction. 相似文献
18.
关于中国沙棘克隆生长调节研究目前局限于外在机制,旨在探讨其克隆生长对灌水强度的响应规律及其激素调控的内在机制。结果表明:随着灌水强度的增大,分株生长、克隆繁殖、克隆扩散能力先升后降,IAA(吲哚-3-乙酸)、ZR(玉米素核苷)、GA_3(赤霉酸)含量及其与ABA(脱落酸)的比值先升后降而ABA含量先降后升。同时,分株生长、克隆繁殖、克隆扩散能力与IAA、ZR、GA_3含量以及IAA/ABA、ZR/ABA、GA_3/ABA呈极显著或显著正相关,与ABA含量呈极显著负相关。灌水强度过小或过大,IAA、ZR、GA_3含量以及IAA/ABA、ZR/ABA、GA_3/ABA低而ABA含量高,克隆生长潜力受到抑制,种群以分株小、数量少(分布稀疏)、扩散(水平根延伸和分枝)能力弱为特征,克隆生长格局倾向于"游击型"、种群早衰概率高;灌水强度适宜,IAA、ZR、GA_3含量以及IAA/ABA、ZR/ABA、GA_3/ABA高而ABA含量低,克隆生长潜力得以充分发挥,种群以分株大、数量多(分布密集)、扩散能力强为特征,克隆生长格局倾向于"聚集型"、种群稳定性高。随着灌水强度过小-适宜-过大的连续变化,中国沙棘通过改变激素状况调控克隆生长,从而形成与灌水强度相适应的克隆生长格局连续体"游击型-聚集型-游击型",种群稳定性呈"低-高-低"的连续变化过程。由此可见:灌水强度诱导内源激素发生改变,激素特征调控克隆生长格局,克隆生长格局决定种群稳定性。 相似文献
19.
During the entire period of internode growth of Merremia emarginata contents of gibberellic acid (GA 3), phenyl-acetic acid (PAA), indole-3-acetic acid (IAA, free and conjugated) and abscisic acid (ABA, free and conjugated)
were estimated by ELISA using polyclonal antibodies raised against each hormones. At the time of internode elongation free
auxin content was low and increased with the decrease in the rate of elongation. In contrast, conjugated IAA showed declining
trend where free IAA content was remarkably high, suggesting thereby that conjugated IAA might have mobilized during the later
phase of internode development. The endogenous GA 3 contents were high as compared to other hormones; however, no significant role of GA 3 was discernible in elongation growth. Conjugated ABA contents remained very low during the elongation growth and increased
thereafter. 相似文献
20.
Fruit set of plants largely depends on the biosynthesis and crosstalk of phytohormones. To date the role of cytokinins (CKs) in the fruit development is less understood. Here, we showed that parthenocarpic fruit could be induced by 1-(2-chloro-4-pyridyl)-3-phenylurea (CPPU, an active CK) in tomato (
Solanum
lycopersicum
cv. Micro-Tom). The fresh weight of CPPU-induced parthenocarpic fruits was comparable with that induced by GA 3. Importantly, CPPU-induced parthenocarpy was found to be compromised by simultaneous application of paclobutrazol (a GA biosynthesis inhibitor), and this effect could be restored by exogenous GA 3. Like pollination, CPPU-induced fruit showed enhanced accumulation of GA 1+3 and indole-3-acetic (IAA), which were accompanied by elevated expression of GA biosynthesis genes like SlGPS, SlGA20ox1, SlGA20ox2 and SlGA3ox1, and IAA biosynthesis gene ToFZY. Elevated GAs level in CPPU-induced fruits was also associated with down-regulation of GA inactivation genes, namely SlGA2ox1,2,3,4,5 in comparison with untreated control. These results suggested that CKs may induce parthenocarpy in tomato partially through modulation of GA and IAA metabolisms. 相似文献
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