共查询到20条相似文献,搜索用时 15 毫秒
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Orchids are the second most diverse plant family, recognized for their importance as ornamental species; this has driven research development in propagation. One of the most common culture methodologies is in vitro asymbiotic germination, in which the nutritional conditions that provide orchids with a fungal partner are emulated. Although Chile possesses more than 60 terrestrial orchid species, in vitro cultivation protocols have only been developed for Chloraea crispa. In Southern Chile, Chloraea gavilu stands out due to its floral characteristics. We evaluate different explants and cultivation conditions for C. gavilu. The best germination and development results were achieved in the MM medium +0.1% yeast extract +1% sucrose +0.454 µmol l?1 TDZ, using immature seeds of 24–30 days after pollination, which we cultivated into seedlings in order to be acclimatized and mycorrhized. In addition, induction of protocorm-like bodies was achieved from germinated seeds, using the same culture media as in the germination and development of immature C. gavilu seeds. This resulted in the successful asymbiotic germination of immature seeds, along with the micropropagation of a terrestrial, temperate orchid. We hope to use our protocol in the commercial production of Chilean orchid species as well as to propagate threatened species. 相似文献
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To our knowledge, this paper is the first record/report of a juvenile light-coloured Weddell seal (Leptonychotes weddellii) at Cape Shirreff, Livingston Island in January 1998, determining that it was an albino individual. Based on available literature,
three cases of albino seals have been reported exclusively for Harbour seal pups, and no albino has been reported for Antarctic
pagophilic true seals. Therefore, this is the first confirmed case of albinism in Antarctic pagophilic true seals species,
indicating that this phenomenon is indeed of a rare occurrence.
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Daniel TorresEmail: |
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A key point in the analysis of dynamical models of biological systems is to handle systems of relatively high dimensions.
In the present paper we propose a method to hierarchically organize a certain type of piecewise affine (PWA) differential
systems. This specific class of systems has been extensively studied for the past few years, as it provides a good framework
to model gene regulatory networks. The method, shown on several examples, allows a qualitative analysis of the asymptotic
behavior of a PWA system, decomposing it into several smaller subsystems. This technique, based on the well-known strongly
connected components decomposition, is not new. However, its adaptation to the non-smooth PWA differential equations turns
out to be quite relevant because of the strong discrete structure underlying these equations. Its biological relevance is
shown on a 7-dimensional PWA system modeling the gene network responsible for the carbon starvation response in Escherichia coli.
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Laurent Tournier (Corresponding author)Email: |
Jean-Luc GouzéEmail: |
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Peter Olofsson 《Biology & philosophy》2008,23(4):545-553
The explanatory filter is a proposed method to detect design in nature with the aim of refuting Darwinian evolution. The explanatory filter borrows
its logical structure from the theory of statistical hypothesis testing but we argue that, when viewed within this context,
the filter runs into serious trouble in any interesting biological application. Although the explanatory filter has been extensively
criticized from many angles, we present the first rigorous criticism based on the theory of mathematical statistics.
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Peter OlofssonEmail: |
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Sub-Antarctic Marion Island has had a permanent research station for 50 years and the islands Wandering Albatrosses have been intensively studied for 20 years. The reactions of breeding birds to approaches by a human on foot were recorded. Three response variables were calculated: intensity of vocal reaction (IVR), intensity of non-vocal reaction (INR) and overall response index (ORI). At 5 m from the nest, twice as many birds stood and/or vocalised as at 15 m. Nearest neighbour distance, age and gender did not explain individual variability of responses. Study colony birds had higher IVR scores than non-study colony birds; birds at colonies closest to the station had the highest ORI scores. A better breeding record was associated with lower IVR and ORI scores, but a causative relationship remains to be demonstrated. A minimum viewing distance of 25 m is recommended for breeding Wandering Albatrosses.
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Marienne S. de VilliersEmail: Fax: +27-21-6503434 |
John CooperEmail: |
Peter G. RyanEmail: |
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Machado RR Lima Filho ES Jardim DF Ferreira MA de Faria CG Duarte RS Lesche B 《European biophysics journal : EBJ》2008,38(1):111-119
An interferometer that measures the refractive index changes due to bacterial metabolism is described. The apparatus permits
simultaneous and real time measurement of bacterial growth in several samples of slowly growing mycobacteria. The error sources
are discussed and the sensitivity of the apparatus is tested. For the species Mycobacterium bovis BCG and M. smegmatis, a relation between refractive index change and bacterial concentration is determined experimentally and the time constant
of bacterial growth is measured.
相似文献
B. LescheEmail: |
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Previous research has shown that two local isolates of bacteria (Pantoea agglomerans and Flavobacterium sp.) are capable of biocontrol of the two main pathogens (Colletotrichum musae and Lasiodiplodia theobromae) known to cause crown rot on “Embul” (Musa, AAB) banana. In this investigation an attempt was made to elucidate the comparative virulence of these pathogens and to
determine the underlying biocontrol mechanisms. L. theobromae was more virulent, causing faster spread of the disease, whereas C. musae was more resistant to the bacterial antagonists. Viable cells of the antagonists were more effective at suppressing conidial
germination than cell-free culture media. It seemed that antifungal compounds acting on conidial germination may be heat stable
and those acting on mycelia may include heat labile compounds also. Considering the specialized roles observed for each pathogen
in terms of causing the disease, and in modes of control by antagonists, future field investigations on biocontrol should
consider the roles played by the pathogens and the antagonists.
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Anjani M. KarunaratneEmail: |
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Methanogens growing on C-1 substrates synthesize 2-carbon acetyl groups in the form of acetyl-CoA for carbon assimilation
using the multienzyme complex acetyl-CoA decarbonylase/synthase (ACDS) which contains five different subunits encoded within
an operon. In species growing on acetate ACDS also functions to cleave the acetate C-C bond for energy production by methanogenesis.
A number of species of Methanosarcina that are capable of growth on either C-1 compounds or acetate contain two separate ACDS operons, and questions have been
raised about whether or not these operons play separate roles in acetate synthesis and cleavage. Methanosarcina thermophila genomic DNA was analyzed for the presence of two ACDS operons by PCR amplifications with different primer pairs, restriction
enzyme analyses, DNA sequencing and Southern blot analyses. A single ACDS operon was identified and characterized, with no
evidence for more than one. MALDI mass spectrometric analyses were carried out on ACDS preparations from methanol- and acetate-grown
cells. Peptide fragmentation patterns showed that the same ACDS subunits were present regardless of growth conditions. The
evidence indicates that a single form of ACDS is used both for acetate cleavage during growth on acetate and for acetate synthesis
during growth on C-1 substrates.
Electronic Supplementary Material Supplementary material is available for this article at
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David A. Grahame (Corresponding author)Email: |
Edward DeMoll (Corresponding author)Email: |
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The primary growth of trees may be studied either by following their development over time, which is costly and requires long-term
monitoring, or by a posteriori growth analyses. Trees in temperate forests show rhythmic growth, which is characterised by
morphological or anatomical markers. The study described here focuses on the pith of trees as an internal marker for the retrospective
analysis of primary growth. Changes in pith size and density were quantified along a stem of Norway spruce [Picea abies (L.) Karst.] with high spatial resolution. The results showed that pith would appear to be a reliable marker of the annual
growth rhythm even in the presence of polycyclism. Annual shoot limits were characterised by reductions in pith size associated,
at the same time, with increases in pith density. In addition, pith size may provide information about tree ontogeny. The
start of the competition with neighbouring trees was very likely responsible for an overall decrease in pith size. Regarding
high frequency variations, pith size appeared to be less sensitive to local environmental fluctuations like climate than other
studied variables such as annual shoot length and annual ring width. Finally, X-ray computed tomography proved to be a very
promising method for the non-destructive detection of annual shoot limits in stems based on longitudinal changes in pith density,
as demonstrated in a log of Norway spruce.
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Yves CaraglioEmail: |
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The recent contribution by Jarmila Kukalová-Peck on Hennigian phylogenetics and hexapod limb evolution is critically evaluated.
相似文献
Michael S. Engel (Corresponding author)Email: |
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Douglas Medin Norbert Ross Douglas Cox Scott Atran 《Human ecology: an interdisciplinary journal》2007,35(3):315-329
There is a continuing controversy over Native American fishing and hunting rights. We show that Native American (Menominee)
and European American fish experts have a common knowledge base and share values and attitudes associated with fishing practices
(though organized around different ethical principles). Nonetheless, perceived group differences are dramatic (especially European American perceptions of Native Americans). Cultural differences in models
of nature and associated inference processes appear to mediate these stereotypes and may hold the key to reducing intergroup
conflict over resources.
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Douglas MedinEmail: |
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Terry Harmer 《Cluster computing》2007,10(3):277-285
Gridcast is an R&D project investigating grid ideas and technologies in the broadcasting technical infrastructure. In this
paper I discuss the business and technical issues in building infrastructures to support broadcasters and outline the structure
of the Gridcast grid-based service oriented architecture for broadcasting playout support.
相似文献
Terry HarmerEmail: |
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Peter Godfrey-Smith 《Biology & philosophy》2007,22(3):429-437
Jablonka and Lamb's claim that evolutionary biology is undergoing a ‘revolution’ is queried. But the very concept of revolutionary
change has uncertain application to a field organized in the manner of contemporary biology. The explanatory primacy of sequence
properties is also discussed.
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Peter Godfrey-SmithEmail: |
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Mycena aphanes and Mycena exilis, belonging to section Filipedes and section Polyadelphia, are proposed as new species. They are described and illustrated and compared to other species in the two sections. Mycena aphanes is a strikingly characteristic Mycena with fulvous or yellowish brown colours of both pileus and stipe. Mycena exilis is characterized by its occurrence on fallen Salix leaves, a pale brown pileus, occasionally with a pale pink tinge, narrowly adnate lamellae, four-spored basidia, and the
presence of clamp connections.
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Gro GuldenEmail: |