沙门氏菌滤过型的研究

根据 B.(1954)的报道,细菌滤遇型早在1907年巳被发现。其后有很多学者进行了这方面的工作。(F.B.Cooper 和 S.A.Petroff,1928;Phillip Hadley 等,1931;H.1925;B.B.CyKaen,B.1937;H.H.1954;B.1954;E.Klieneberger-Nobel,1951;I.Nasz 和 B.Lovas,1956)。近年来滤过型存在的重要性更引起了医学家与生物学家的普遍注意;但是这种形态在生物学上与免疫学上的意义,直到现在,还不够清楚。我们于1955年开始沙门氏菌中的伤寒桿菌及乙型伤寒桿菌滤过型问题的研究。     

RAPID IDENTIFICATION AND SEROTYPING OF SALMONELLA ENTERITIDIS

A latex agglutination kit for S. enteritidis was evaluated on 155 field isolates from 29 serotypes from Denmark, All but one of the S. enteritidis isolates were correctly identified by the kit, and none of the isolates of other serotypes tested were identified as S. enteritidis. The results indicate a very high degree of specificity and sensitivity for the SEF14 monoclonal antibody, which forms the basis of the test.     

伤寒杆菌和甲型副伤寒杆菌的稳定L型外膜蛋白和染色体DNA酶切研究

非高渗培养基传代培养的伤寒杆菌和甲型副伤寒杆菌的稳定L型丧失了主要外膜蛋白、特异性表面抗原和染色体DNA部分片段,保留了沙门氏菌共同的内部抗原和形成L型独特的表面抗原。提示伤寒杆菌和甲型副伤寒杆菌的稳定L型多种特性的丧失同其基因或主要外膜蛋白的缺失有关。     

鼠伤寒沙门氏菌Ⅰ相鞭毛蛋白基因fliC~I的克隆和鉴定

以提纯的鼠伤寒沙门氏菌8705染色体DNA为材料,经EcoR Ⅰ消化,过SepharcylS-400柱,得到大于400bp的酶切片段;然后随机克隆到质粒pGEM-3Zf(—)中,转化大肠杆菌LC2a(hag~-,recA~-);在氨苄青霉素平板上共得到6013个转化子,从中筛选出1个有动力的克隆,小量制备质粒DNA,经酶切电泳鉴定,该克隆的外源片段大小为15.3kb,将其命名为pGI4015。动力、动力抑制试验和Southern blot分子杂交试验证明pGI4015中载有鼠伤寒沙门氏菌Ⅰ相鞭毛蛋白基因fliC~I;利用其BamH Ⅰ和Sal Ⅰ位点删除与鞭毛蛋白表达无关的序列,构建亚克隆质粒pGI4015BS,使得fliC~I定位于更小的区域——3.8kb的BamH Ⅰ/Sal Ⅰ插入片段上。     

COMPARISON OF MODIFIED SALMONELLA-TEK ENZYME IMMUNOASSAY AND MODIFIED GENE-TRAK SALMONELLA ASSAY FOR RECOVERY OF SALMONELLA FROM SELECTED LOW-MOISTURE FOODS

The sensitivity of the original versus the modified Salmonella-Tek enzyme immunoassay (EIA) was compared for recovery of Salmonella spp. from selected low-moisture foods. Serial tenfold dilutions were inoculated into incubated post enrichment media (dilution-to-extinction approach). Results indicated no significant difference between the original and modified Salmonella-Tek EIAs. A comparison of the modified Salmonella-Tek EIA and the modified GENE-TRAK Salmonella assay, using the same approach, also showed no significant differences.
The effectiveness of the assays was then compared using a second approach (dry bulk inoculation). A lyophilized culture was inoculated into bulk food. Replicate 25-g test portions were used in a 3-way comparison of the effectiveness of the modified Salmonella-Tek EIA, the modified GENE-TRAK Salmonella assay, and the standard culture method approved by the Association of Official Analytical Chemists (AOAC) International and recommended in the Food and Drug Administration's Bacteriological Analytical Manual. Of 460 test portions examined, 307 gave positive reactions with the modified Salmonella-Tek EIA, 298 with the modified GENE-TRAK Salmonella assay, and 292 with the AOAC/BAM culture method.     

GENETIC HOMOLOGY BETWEEN ESCHERICHIA COLI K-12 AND SALMONELLA.

Falkow, Stanley (Walter Reed Army Institute of Research, Washington, D.C.), Robert Rownd, and L. S. Baron. Genetic homology between Escherichia coli K-12 and Salmonella. J. Bacteriol. 84:1303-1312. 1962.-Recombinant analysis and interrupted mating procedures, in conjunction with molecular hybridization experiments, demonstrated that the genetic homology between Escherichia and Salmonella is incomplete. The most likely explanation of this incomplete homology is imperfect pairing between the deoxyribonucleic acid molecules of the two species. Despite the inhomologies, there is ample evidence that the order and distance of the genetic characters on the Salmonella chromosome are identical to those of Escherichia.