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1.
New Zealand white rabbits were immunized with partly fed Hyalomma dromedarii tick-derived midgut concealed antigens (supernate and pellet fractions) and Freund's complete adjuvant (FCA). The rabbits received three inoculations subcutaneously on days 0, 14 day 21 at a dose rate of 1 mg antigen per animal. The effects of the immunity induced was determined by infesting the rabbits with adult H. dromedarii ticks. In immunized rabbits a significant reduction in tick yield, engorgement weight, oviposition period, egg mass weight and percentage of egg hatchability was found. The gut supernatant antigen fraction induced the best protection in terms of reduced feeding and reproductive performance of the ticks. 相似文献
2.
D. BEN-YAKIR K. Y. MUMCUOGLU O. MANOR J. OCHANDA† R. GALUN 《Medical and veterinary entomology》1994,8(2):114-118
Abstract. Resistance to human body lice, Pediculus humanus humanus L, induced by feeding on rabbits immunized with an extract of louse gut was studied. The mortality of lice fed on immunized rabbits was 73%, significantly higher than that of lice fed on control rabbits (52%) ( P < 0.01). The proportion of dead nymphs and female lice with ruptured guts was significantly higher in lice fed on immunized rabbits (P<0.01). The size of the bloodmeal was 35% greater in female lice fed on control rabbits than on immunized rabbits. Lice fed on immunized rabbits laid 40% less eggs than those fed on the controls, they also demonstrated a significant decrease in the number of eggs per female over time ( P < 0.01). 86% of the eggs laid by lice fed on immunized animals hatched, compared with 92% hatching of eggs laid by the lice fed on control animals (P< 0.01). With the exception of the first bloodmeal the percentage of hatched eggs which were laid between any two bloodmeals was significantly smaller (p<0.01) in the lice fed on immunized rabbits than in the control group. The first nymphal stage of lice fed on immunized rabbits took an average of 5.2 days to moult to the second stage, compared with 4 days for those fed on control rabbits. 相似文献
3.
《Journal of insect physiology》1987,33(6):379-383
The possiblity of suppressing the growth of Nocardia (Rhodococcus) rhodnii symbiont in the gut of Rhodnius prolixus by feeding the bugs on rabbits immunized against the bacterium, was investigated. Rabbits immunized with symbionts isolated from adult bugs and grown in vitro developed high antisymbiont titres (1,024-2,048) and showed no ill side effects. The log-phase growth of the symbionts was retarded in the gut of R. prolixus larvae fed on immunized rabbits. Both growth rate and maximal number of symbionts were lower in groups receiving treatment. Rate of development of bugs reared on immunized rabbits was slowed and their success in moulting was reduced. 相似文献
4.
AdultRhipicephalus appendiculatus ticks were fed as three sequential infestations on both rabbits and cattle. The feedings at first infestation on naive hosts were optimum for the ticks, whereas at third infestation the hosts were resistant, as expressed by reduced tick feeding performance. Ticks from naive and resistant hosts were examined for histological differences of salivary glands. In ticks fed on resistant rabbits there was a large increase in the synthesis of glycoprotein secretory granules in thec
1 cells compared with ticks fed on naive rabbits. In ticks fed on naive and resistant cattle, the activity of thec
1 cells was less than in ticks fed on naive and resistant rabbits. It was concluded that the salivary glands are able to respond selectively to conditions at the feeding site, and that this may be advantageous to the tick. 相似文献
5.
The Effect of Male Ticks on the Feeding Performance of Immature Stages of Rhipicephalus Sanguineus and Amblyomma Americanum (Acari: Ixodidae) 总被引:3,自引:0,他引:3
A unique group of immunoglobulin-binding proteins (IGBPs), produced by ixodid male ticks during the latter half of their prolonged feeding period, improves the feeding performance of co-feeding females. As a follow-up to this observation, we investigated whether male tick feeding also affects the feeding of other developmental stages. Immature stages of Rhipicephalus sanguineus (Latreeille) and Amblyomma americanum (L.) were fed on rabbits in the presence or absence of conspecific males. The mean weight of larvae and nymphs of both species that fed around males and detached from the host on the first day of dropping was significantly higher than when the immature ticks fed on rabbits in the absence of males. However, larvae of both species and nymphs of R. sanguineus that fed slower and detached on the second day of dropping did not show significant differences in weight. A similar pattern was observed for A. americanum nymphs although, unlike R. sanguineus, the presence of males also influenced the feeding performance of the nymphs that fed slowly and detached on the second day of drop-off. The improved feeding performance demonstrated by immature ticks in the presence of males may be due to immunomodulatory saliva proteins, such as immunoglobulin-binding proteins (IGBPs) that are introduced into the co-feeding site. The results are considered in relation to the distribution of ixodid tick species on their natural hosts. 相似文献
6.
Jongejan F. Pegram R. G. Zivkovic D. Hensen E. J. Mwase E. T. Thielemans M. J. C. Cossé A. Niewold T. A. Said Ashrafel Uilenberg G. 《Experimental & applied acarology》1989,7(3):181-199
The ability of rabbits, goats and cattle to acquire immunity to the ixodid ticksAmblyomma variegatum andRhipicephalus appendiculatus was studied under laboratory and field conditions. Rabbits were successfully immunized with crude salivary gland extract (SGE) and midgut extract (ME) obtained from flat or partly fed femaleR. appendiculatus ticks. The lowest numbers of larvae were produced by females fed on rabbits immunized with unfed midgut extract. Similar reductions in larval production could be induced after three infestations of rabbits with adultR. appendiculatus. Also, successive feedings of nymphs ofR. appendiculatus on rabbits resulted in significantly reduced engorgement weights. Skin testing with SGE induced delayed-type hypersensitivity reactions, which could be correlated with immunity toR. appendiculatus in rabbits. Moreover, circulating antibodies were detected in rabbits with an ELISA using SGE ofR. appendiculatus.Immunity toA. variegatum nymphs could be induced in rabbits by repeated infestations, but this failed in goats. Immunization of goats with midgut extract from adultA. variegatum did not protect against subsequent nymphal challenge, but strong skin reactions were noticed when adults ticks fed on immunized goats. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of SGE and ME fromA. variegatum revealed the presence of 48 protein bands in SGE and 29 bands in midgut extract. Western blotting employing serum from a rabbit immune toR. appendiculatus recognized a number of bands in SGE fromR. appendiculatus, but also in SGE ofA. variegatum.Immunity acquired by cattle to ixodid tick infestations under field conditions was monitored by skin testing with SGE and western blot analysis. In general, cattle with the lowest tick numbers manifested the strongest delayed-type hypersensitivity responses. Finally, western blot analysis employing sera from tick-infested and tick-naive cattle could not be related to actual immune status. 相似文献
7.
Aposymbiotic tsetse flies, Glossina morsitans morsitans obtained by feeding on rabbits immunized specifically with symbionts 总被引:1,自引:0,他引:1
G Nogge 《Journal of insect physiology》1978,24(4):299-304
Tsetse flies fed on rabbits which previously had been immunized with whole flies without symbionts showed an increase in mortality and only a small decrease in fecundity. Flies fed on rabbits immunized with symbionts only became aposymbiotic. Their fecundity decreased drastically while their longevity was not affected. The antibodies found in the flies were specifically attached to the tissues which had been used as antigens. 相似文献
8.
Dispersal capacity of Haematopota spp. and Stomoxys calcitrans using a mark–release–recapture approach in Belgium
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L. Lempereur C. Sohier F. Smeets F. Maréchal D. Berkvens M. Madder F. Francis B. Losson 《Medical and veterinary entomology》2018,32(3):298-303
The dispersion potential of mechanical vectors is an important factor in the dissemination of pathogens. A mark–release–recapture experiment was implemented using two groups (unfed and partially fed) of the Tabanidae (Diptera) (Haematopota spp.) and biting Muscidae (Diptera) (Stomoxys calcitrans) most frequently collected in Belgium in order to evaluate their dispersion potential. In total, 2104 specimens of Haematopota spp. were collected directly from horses and 5396 S. calcitrans were collected in a cattle farm using hand‐nets. Some of these insects were partially fed in vitro and all were subsequently coloured. Overall, 67 specimens of S. calcitrans (1.2%) and 17 of Haematopota spp. (0.8%) were recaptured directly on horses. Stomoxys calcitrans flew maximum distances of 150 m and 300 m when partially fed and unfed, respectively. Haematopota spp. travelled maximum distances of 100 m and 200 m when partially fed and unfed, respectively. Segregation measures seem essential in order to reduce the risk for pathogen transmission. A distance of 150 m appears to be the minimum required for segregation to avoid the risk for mechanical transmission, but in areas of higher vector density, this should probably be increased. 相似文献
9.
Y. Rechav S. Mnqandi E. T. Mwase A. Heller-Haupt M. G. R. Varma 《Experimental & applied acarology》1991,11(4):317-321
Guinea-pigs infested with male ticks of the speciesRhipicephalus appendiculatus, and rabbits infested withR. evertsi evertsi, acquired immunity to conspecific female ticks. The hosts were first infested with male ticks and thereafter were challenged with males and females of the same species. The mean weight of the engorged females ofR. appendiculatus fed on guinea pigs previously infested with male ticks was 509.0 (±41.4) mg compared with that of females fed on control guinea pigs (651.2±31.8 mg). Similar weight differences were observed forR.e. evertsi females which fed on rabbits previously infested three times with male ticks. The mean weight of the female ticks which fed on these rabbits was 520.1 (±29.8) mg compared with 640.7 (±30.2) mg ofR.e. evertsi females which fed on control hosts. The concentration of gammaglobulins in the sera of rabbits was monitored at various intervals after the first infestation. It was found, for the first time, that infestation of laboratory animals with male ticks conferred immunity, but to a lesser degree than infestation with both sexes. It was also shown that the level of gammaglobulins increased from 3.4±0.28 g l–1 to 7.3±0.24 g l–1 in sera of rabbits hosts as a result of the feeding activity of males, but to a lesser extent than in sera of rabbits on which both sexes had fed (10.8±2.4 g l–1). 相似文献
10.
P. H. Nunes G. H. Bechara M. I. Camargo Mathias 《Experimental & applied acarology》2011,53(2):179-187
Ticks have great economic and health importance since infested animals have reduced milk and meat production, and, besides that, they are expensive ectoparasites to control. While feeding, ticks can transmit to their hosts a large amount of pathogens, including Rickettsia rickettsii responsible for the ??spotted fever?? or ??fever of the mountains.?? It is known that animals infested with ticks or artificially immunized with their salivary gland extracts develop resistance, which is related to a decrease in engorged female weight, in egg-laying by adults, in egg viability and, in some cases, in the capacity of pathogens transmission. The present study aimed to examine morpho-histochemically the female salivary glands of semi and engorged Amblyomma cajennense fed on resistant rabbits. The results revealed that acinus I had no changes when compared to that of females fed on naive rabbits. The c cells of acinus II showed signs of early degeneration, which may result in feeding efficiency decrease. In acinus III d cells, activity time was longer. Such occurrence was associated with the time of female fixation, which increased in females fed on resistant hosts. 相似文献
11.
The effect of lipid lowering agents of plant origin garlic oil and guggulipid on the levels of catecholamine and dopamine
Β-hydroxylase activity of normal and cholesterol fed rabbit tissues has been studied. The catecholamine levels and enzyme
activity were found to be decreased in cholesterol (500 mg/kg body wt) fed animals. The feeding of garlic oil (5 mg/kg body
wt) and guggulipid (100 mg/kg body wt) an exudate ofCommiphora mukul, to normal rabbits caused significant increase in the dopamine-Β-hydroxylase activity and catecholamine levels, while the
feed helped the hypercholesterolemic rabbits to recover the decrease in catecholamine biosynthesis
C.D.R.I. Communication No. 3435. 相似文献
12.
Immunizations of New Zealand White rabbits with specific macrophage migration inhibitory factor (MIF) tick peptide (PEP) produced circulating anti-tick PEP antibodies in the hosts. Antibody titers of greater than 1:5000 to tick MIF peptide were observed for crude sera from PEP-immunized rabbits. PEP- and BSA-vaccinated rabbits were infested with Amblyomma americanum adults. Feeding intervals, female weights, egg masses and percent egg hatch were measured for ticks feeding on control and immunized hosts. Feeding intervals were significantly lengthened to 13.3 days for PEP-vaccinated hosts compared to BSA-vaccinated controls at 12.4 days, while female engorgement weights and egg masses were unchanged. By immunizing hosts using specific tick PEP, we were able to alter the length of time the ticks fed on their hosts. 相似文献
13.
K. Y. MUMCUOGLU D. BEN-YAKIR J. O. OCHANDA J. MILLER R. GALUN 《Medical and veterinary entomology》1997,11(4):315-318
Abstract Immunization of rabbits with a faecal extract of the human body louse (Pediculus humanus) induced a high titre of specific IgG. The mean weight of Wood taken by females fed on the immunized rabbits was significantly lower (29%) than taken by females fed on the control rabbits. The mean number of eggs per female fed on the immunized rabbits was significantly lower than for females fed on the control rabbits. The hatchability of the eggs laid by lice fed on immunized rabbits (91%) was significantly lower than of those fed on control rabbits (94%). The rate of development of nymphs fed on control rabbits was significantly higher than those fed on the immunized rabbits. There was no difference in survival rates of lice fed on immunized and control rabbits. 相似文献
14.
In this study we examine the feeding patterns of immature stages of Hyalomma truncatum and Hyalomma marginatum rufipes ticks on different hosts. Larvae of H. truncatum developed through a three-host pattern on two species of field mice, Rhabdomys pumilio and Lemniscomys rosalia. On guinea-pigs, both Hyalomma species followed a mixed two-host and three-host pattern, with the latter route being preferred, since more than 70% of the fully fed larvae dropped off from their hosts. H. truncatum was a two-host tick on rabbits. Larvae of H. marginatum rufipes did not prefer R. pumilio and L. rosalia as hosts. On guinea-pigs, H. marginatum rufipes immatures showed a mixed two-host and three-host pattern with a bias towards the three-host life cycle, since approximately 58% of the fully fed larvae dropped off. On rabbits, H. marginatum rufipes was exclusively a two-host tick. Mean engorgement weights and blood quantities ingested by H. truncatum nymphs that developed through a three-host pattern on mice were significantly higher (p<0.0001) than for those that developed through a two-host pattern on guinea-pigs and rabbits. For H.marginatum rufipes, there were no significant differences (p>0.05) between engorgement weights of nymphs that developed through two-host and three-host patterns. However, there were significant differences (p<0.0001) in blood quantities ingested by nymphs of this tick species following feeding on different hosts. 相似文献
15.
G. S. Ba$nTuelos D. B. Vickerman J. T. Trumble M. C. Shannon C. D. Davis J. W. Finley 《International journal of phytoremediation》2002,4(4):315-329
We are investigating the biotransfer of accumulated Se by the plant in several phytoremediation systems. In study I, we evaluated the biotransfer of Se from Indian mustard, a Brassica species, to the insect-cabbage looper (Trichoplusia ni); mortality, deterrence, and biomagnification of Se were examined. We determined that feeding behavior of food chain consumers was affected not only by the plant concentration of Se, but also by the mobility of the insects and choice of feed available. In study II, we examined the survival and development of beet army worm (Spodoptera exigua) fed Se-enriched plant tissues from different lines of saltbush (Atriplex spp.) After feeding on lines of saltbush that produced high biomass and accumulated high concentrations of Se, insect growth and survival was reduced. In studies III, IV, and V, lambs, dairy cows, and rabbits were fed Se-enriched Brassica and Medicago (alfalfa) plants as part of their feed ration. None of the tested animals exhibited any Se toxicity symptoms, but they had increased levels of Se in most tissues sampled (e.g., organs, blood, urine, feces), excluding milk. In study VI, we evaluated biotransfer of Se from broccoli to rats to determine efficacy of Se for reducing colon cancer. We found that Se-enriched plant material was more effective than inorganic sources of Se for preventing precancerous colon lesions. Results from all studies clearly show that Se absorbed by plants can be transferred biologically in an intentional or unintentional manner to insects and animals. 相似文献
16.
The effect of anti-mosquito-midgut antibodies on the development of the malaria parasite, P. vivax was studied by feeding the vector mosquito, An. culicifacies with infected blood supplemented with serum from immunized rabbits. In order to get antisera, rabbits were immunized with midgut proteins of three siblings species of Anopheles culicifacies, reported to exhibit differential vectorial capacity. The mosquitoes that ingested anti-midgut antibodies along with infectious parasites had significantly fewer oocysts compared to the control group of mosquitoes. The immunized rabbits generated high titer of antibodies. Their cross reactivity amongst various tissues of the same species and with other sibling species was also determined. Immunogenic polypeptides expressed in the midgut of glucose or blood fed An. culicifacies sibling species were identified by Western blotting. One immunogenic polypeptide of 62 kDa was exclusively present in the midgut of species A. Similarly, three polypeptides of 97, 94 and 58 kDa and one polypeptide of 23 kDa were present exclusively in species B and C respectively. Immunoelectron microscopy revealed the localization of these antigens on baso-lateral membrane and microvilli. The effects of anti-mosquito midgut antibodies on fecundity, longevity, mortality and engorgement of mosquitoes were studied. Fecundity was also reduced significantly. These observations open an avenue for research toward the development of a vector-based malaria parasite transmission-blocking vaccine. 相似文献
17.
Biological characteristics of Hyalomma rufipes parasitising on rabbits and sheep were compared under laboratory conditions in Gansu, China. Mature ticks could parasitize
both rabbits and sheep, while immature ticks only fed on rabbits successfully. Adults sucked blood on sheep significantly
longer than on rabbits (16 and 13 days, respectively). Other adult parasite characteristics fed on the two hosts were similar,
including the weight of engorged adult, female daily oviposition, and the weight and amount of the egg mass laid. Those indicated
that this tick species showed little host specificity between sheep and rabbits during its adult stage. In total, the life
cycle of H. rufipes was completed in an average period of 179 days. The average developmental periods were 59 days for egg incubation, 3 and
21 days for immature tick prefeeding and feeding, 2, 12 and 40 days for adult prefeeding, female preoviposition and oviposition.
The longer female fed for engorgement, the shorter preoviposition period of engorged female needed, although when the feeding
period was less than 15 days, this relationship was not obvious. The results confirmed the correlation between the weight
of the engorged female and the number of eggs laid (r = 0.909). The reproductive efficiency index (REI) and reproductive fitness index (RFI) in females was 10.63 and 7.22, respectively.
Engorged nymphs moulting to females were significantly heavier (27.6 ± 0.89 mg) than those moulting to males (22.3 ± 0.52 mg).
Males outnumbered females by 1.4:1. 相似文献
18.
1. The selective feeding hypothesis predicts that miners prefer tissues high in nutrient content and low in chemical and structural defence content. This hypothesis was tested for two dipteran grass miners, Chromatomyia milii and C. nigra, feeding on Holcus lanatus. 2. Leaf sections revealed that both miners preferred to feed on the mesophyll layer, minimised the intake of veinal tissues, and never fed on the epidermal layer. Nutritional quality analyses showed that the mesophyll had a higher nutrient content and a lower structural defence content than the epidermal and veinal tissues. 3. The nutritional quality of mined leaf areas was lower than that of unmined leaf areas, suggesting that the mining larvae remove the most nutritious tissues; however this commonly used approach for studying the selective feeding hypothesis is questioned in this study. 4. Larval feeding rates were higher on the mesophyll than on the veinal tissues, probably due to a higher structural defence content of the veins. Feeding rates were higher on the small veins than on the larger and more sclerified, strengthened veins. 5. This study supports the selective feeding hypothesis as both species concentrated their feeding on the mesophyll, which is the most nutritious and least tough tissue within a H. lanatus leaf. 相似文献
19.
Ewuola EO Gbore FA Ogunlade JT Bandyopadhyay R Niezen J Egbunike GN 《Mycopathologia》2008,165(2):99-104
Maize grains contaminated with fumonisin, a metabolite of Fusarium
verticillioides was incorporated into matured male rabbits’ diet to evaluate its effects on performance, haematology and serum biochemistry
in rabbits. Thirty individually caged crossbred adult rabbit bucks averaging 1.36 ± 0.01 kg (about 22–24-week-old) were randomly
allotted to three treatment diets comprising a control diet (containing 0.35 ± 0.02 mg fumonisin/kg) and two test diets containing
12.30 ± 0.16 and 24.56 ± 0.14 mg fumonisin/kg, constituting treatments 1 (low infection), 2 (medium infection) and 3 (high
infection), respectively, in a five-week feeding trial. Results showed that the dry matter intake (DMI) (g/rabbit) at the
end of the feeding trial was significantly (P < 0.05) influenced. The DMI declined with increasing dietary fumonisin by a significant 80% and 95% (P < 0.05) for high and medium levels of dietary fumonisin, respectively, relative to the mean weekly DMI of 609.93 ± 45.08 g
by rabbits fed diet with low level of fumonisin. The weekly weight gain tended to decrease with increased dietary fumonisin
levels, while the haematological and serum biochemical components examined, were not statistically influenced among the diets
when fed to male rabbits for a period of 5 weeks.
Target audience: Livestock farmers, Feed millers, and Animal nutritionists. 相似文献
20.
Abstract. Stable flies (Stomoxys calcitrans L.) deprived of a bloodmeal until 3 days post-emergence had higher mortality rates than control flies fed from the day of emergence. Fat bodies of deprived females required one more bloodmeal to reach maximum size, and maximum size was smaller, than fat bodies of control females. Ovarian development did not commence prior to feeding in deprived flies, and proceeded more slowly thereafter, resulting in a one bloodmeal delay in egg maturation in deprived flies. Deprived females produced fewer (54.7, SD 2.8) eggs than controls (75.9, SD 3.7) and eggs from deprived females were smaller (mean length 684.0 μrn) than control females' eggs (mean length 1165.7 μm). 相似文献