子囊菌亚门石耳科属级分类及拉诺属地位的研究

关于子囊菌亚门石耳科的属级分类问题地衣学家持有不同的观点。这些观点使属级分类出现了单属系统,二属系统,三属系统以及四属系统与五属系统。现在新二属系统,即石耳属一疱脐衣属系统已被越来越多的地衣学家和子囊菌学家所接受。但是,因为由Dodge (1968)描述的单种属拉诺属(Llanoa)的模式种与早已为人熟知的网脊石耳很相似,而近年来,这一单种属又被一些作者所接受、因此、我们不得不对该属模式种所依据的原始材料进行必要的复查。复查结果表明,拉诺属的模式种卖为网脊石耳的异名。因此,拉诺属也就成为石耳属的异名。此外,基于石耳科中三十五种地衣和五十二项特征的聚类分析结果也支持新的二属系统。     

THE PLATYCARYA PERPLEX AND THE EVOLUTION OF THE JUGLANDACEAE

We report on the leaves, fruits, inflorescences, and pollen of two fossil species in the genus Platycarya. The association of these dispersed organs has been established by their repeated co-occurrence at a large number of localities, and for two of the organs (fruit and pistillate inflorescence, and pollen and staminate inflorescence) by apparent organic attachment of compression fossils. Each of the two species can be distinguished by characteristics of all the known megafossil organs. We also review the fossil record of dispersed platycaryoid fruits and inflorescences, recognizing three additional species of Platycarya and two of Hooleya. Two of the fossil Platycarya species are morphologically very different from the living Platycarya strobilacea Sieb. et Zucc., but they show the diagnostic features of the genus. Hooleya is a generalized member of the Platycaryeae that is probably close to the ancestry of Platycarya. The two Platycarya species known from multiple organs provide a remarkable example of mosaic evolution in which fertile and foliar structures have attained different levels of morphological specialization. The leaves, often considered the most plastic of plant organs, retain several features that are otherwise seen only in the Engelhardieae. These similarities in leaf architecture between the fossil Platycarya species and Engelhardieae are advanced features for the Juglandaceae, and thus indicate a sister-group relationship between the two lines. In contrast to the leaves, the fruits, inflorescences, and pollen of the fossil Platycarya species are almost as specialized as those of the extant P. strobilacea and bear little resemblance to the same structures in other genera of the family. The morphology, taphonomy, sedimentary setting, and geographic and stratigraphic distribution of three of the fossil platycaryoid species suggest that they were wind-dispersed, early successional plants that grew in thickets. This habit is retained by Platycarya strobilacea and is typical of many of the amentiferae (e.g. Myricaceae, Betulaceae). The r-selected life-history pattern of the Platycarya line may well have contributed to its low diversity through geologic time.     

THE ORGANIZATION OF THE VASCULAR SYSTEM IN THE STEMS OF THE NYMPHAEACEAE. III. VICTORIA AND EURYALE

Organization of the stem vascular system was analyzed in Victoria species and Euryale ferox. The stem vascular system consists of a number of concentrically-organized continuing axial stem bundles. At the node each leaf is supplied with a root trace, two lateral leaf traces, and a median leaf trace. A peduncle fusion bundle is also present at each node. The peduncle fusion bundle supplies vascular tissue to the median leaf trace and to the peduncle trace. Flowers are nonmedian axillary but have specific vascular, spatial, and developmental relationships to leaves in a manner that resembles the genus Nymphaea. On the basis of the analysis of the stem vascular system, Victoria and Euryale are more similar to each other than to Nymphaea. However, the vascular system in Victoria and Euryale is similar enough to Nymphaea to suggest that Nymphaea, Victoria, and Euryale form a natural taxon of unique angiosperms. The organization of the stem vascular system in Victoria and Euryale is dicotyledonous.     

ANTHER MORPHOLOGY AND THE ORIGIN OF THE TETRAPLOID WHEATS

Anther morphology of various species of the genus Triticum is consistent with previous evidence that different biotypes of T. boeoticum (AA) and T. urartu (BB) contributed the respective A and B genomes to the emmer (A^eA^eB^eB^e) and timopheevi (A^tA^tB^tB^t) tetraploid complexes. Anther length of T. dicoccoides (2.8 mm) and T. araraticum (3.0 mm) is mid-way between that of T. boeoticum (3.6) and T. urartu (2.2) and the same as that of the sterile hybrid T. boeoticum X T. urartu. With respect to mode of dehiscence, post anthesis reflexion of anther lobes and twisting of the anthers, T. dicoccoides resembles T. boeoticum, whereas T. araraticum resembles T. urartu. With respect to anther apex, T. dicoccoides resembles neither T. boeoticum nor T. urartu but is identical with their F₁ hybrid. Among amphiploids involving four different Aegilops species and T. boeoticum lines, none could similarly account for the length or other diagnostic attributes of the tetraploid anthers. Anther morphology is consistent with the presumed genomic composition of the cultivated tetraploid and hexaploid wheats and seems to distinguish effectively the different genomic groups of the genus Triticum.     

THE DISTRIBUTION AND TAXONOMIC SIGNIFICANCE OF LIGNIN IN THE LEMNACEAE

Lignification characteristics of 14 species of Lemnaceae were studied by the alkaline oxidation technique in an attempt to resolve conflicting reports of the presence of lignin and to provide chemotaxonomic data for supplementing previous systematic treatments. Four species of Spirodela, eight of Lemna, Wolffiella oblonga, and Wolffia microscopica were clonally subcultured, homogenized, exhaustively extracted and the insoluble residue oxidized in alkaline cupric hydroxide. Barley (Hordeum vulgare), Elodea densa and Spirogyra sp. were examined to validate the technique. The benzaldehydes were extracted, separated by thin-layer chromatography, and quantitated spectrophotometrically. Recovery of syringaldehyde, vanillin, and to a lesser extent p-hydroxybenzaldehyde, was accepted as evidence that these plants are lignified. The highest yields of syringaldehyde and vanillin were recovered from S. intermedia, the largest member of the Lemnaceae. Lesser amounts were obtained from S. polyrhiza and S. oligorhiza. Spirodela biperforata yielded no syringaldehyde which substantiates its distinctiveness from the morphologically similar S. polyrhiza. All Lemna species are nerved, yet vanillin (but not syringaldehyde) was recovered only from L. trinervis, L. perpusilla, L. minor, and L. minima. The species L. trisulca, L. gibba, L. obscura, and L. valdiviano yielded only p-hydroxybenzaldehyde. Thus the presence of nerves is not evidence for lignification within the genus Lemna, and the relationships suggested by their gross morphology are not supported by their lignin chemistry. Wolffiella is non-lignified by these criteria. Wolffia microscopica is the smallest flowernig plant and it has no nerves; vanillin was unexpectedly recovered and microscopic examination showed highly lignified anther walls.     

THE CYTOLOGY AND PHYLOGENETICS OF THE DIPLOID SPECIES OF GOSSYPIUM

Meiotic chromosome behavior of 11 inter-genomic hybrids of Gossypium (2n = 26) were investigated. Per cell univalent frequencies at meiotic metaphase I in these hybrids were: A genome × Cgenome—G. herbaceum × sturtianum, 10.53; G. herbaceum × australe, 18.05. A genome × E genome—G. smnalense × arboreum, 21.82. B genome × C genome—G. anomalum × sturtianum, 9.23; G. anomalum × australe, 13.11. B genome × D genome—G. anomalum × klotzschianum, 17.45; G. anomalum × raimondii, 18.83. C genome × D genome—G. robinsonii × davidsonii, 12.77; G. sturtianum (armourianum × thurberi), 8.63. C genome × E genome—G. somalense × australe, 23.78; G. somalense × bickii, 25.58. Trivalent and quadrivalent frequencies were relatively high for those hybrids involving a C genome species, indicating that a reciprocal translocation differentiates the C genome from the A, B, D, and E genomes. The results of this study and the data of similar studies cited from the literature on Gossypium cytogenetics are discussed relative to the phylogenetics and evolution of the major (genome) groups of Gossypium and their constituent taxa.     

SOME NOTES ON THE BIRDS OF THE ISOKA DISTRICT OF THE NORTHERN PROVINCE OF NORTHERN RHODESIA

Dean, W. R. J. 1978. Moult seasons of some Anatidae in the western Transvaal. Ostrich 49:76-84.

Spurwinged Geese Plectropterus gambensis, Egyptian Geese Alopochen aegyptiacus, Yellow-billed Ducks Anas undulata, Redbilled Teal A. erythrorhyncha and Southern Pochard Netta erythrophthalma have a flightless moult mainly during the dry season, from April to August, in the western Transvaal. South African Shelduck Tadorna cana moult during October to February after breeding during July and August. The Cape Shoveller Anas smithii has two main flightless periods, April-May and October-January. Cape Teal A. capensis have been recorded in flightless moult in October, December and January.

The duration of the flightless period correlates with wing length; larger and longer winged Anatidae require proportionally more time for wing moult than do smaller and shorter winged Anatidae.

Geese and shelducks moult on large open lakes with an open shore. Ducks have been recorded flightless on lakes and dams, with or without emergent vegetation.     

CHROMOSOME NUMBERS FROM THE FLORA OF THE JUAN FERNANDEZ ISLANDS

Forty-one chromosome counts are reported for 29 species of the flora of the Juan Fernandez Islands, Chile. Counts are provided for the first time for Azara (Flacourtiaceae), Cuminia (Lamiaceae), Dysopsis (Euphorbiaceae), Raphithamnus (Verbenaceae), Robinsonia (Asteraceae) and Ugni (Myrtaceae). New species counts are in Dendroseris and Erigeron (Asteraceae), Eryngium (Apiaceae), Escallonia (Saxifragaceae), Haloragis (Haloragaceae), Libertia (Iridaceae), Myrceugenia (Myrtaceae), Pernettya (Ericaceae), Peperomia (Piperaceae), Spergularia (Caryophyllaceae), and Wahlenbergia (Campanulaceae). The level of recent polyploidy and aneuploidy in the chromosomally known flora is each estimated conservatively at 6%. Almost all of the endemic lineages appear to have evolved at the same ploidy level from their polyploid mainland relatives. Furthermore, there is no evidence for chromosomal evolution by euploidy or aneuploidy within endemic genera. The level of ancient polyploidy in the endemic Juan Fernandez flora is estimated to be 66%. This high level correlates with the idea that many of the woody endemics (comprising nearly one-half of the woody species in our chromosomal sample) have been derived paedomorphically from herbaceous perennial mainland ancestors in temperate, orogenetically active, Andean regions, in which high levels of polyploidy would be expected.     

THE VERTEBRATE FAUNA OF THE PRIMAEVA BEDS OF THE FRæNKELRYGGEN FORMATION OF VESTSPITSBERGEN AND ITS BIOSTRATI-GRAPHIC SIGNIFICANCE

New occurrences of Traquairaspis sp., Oniscolepis? sp., Turinia sp., Cephalaspis (Zenaspis) cf. excellens Wängsjö, Nostolepis sp. and Ischna-canthidae gen. et sp. indet. are recorded from the primaeva Beds. Taken as a whole, the vertebrate fauna of these beds shows a general resemblance to those of the upper part of the symondsi zone and the lowermost part of the leathensis zone in the Welsh Borderland. For this and other reasons it is suggested that the Frænkelryggen Formation should be correlated approximately with the symondsi and leathensis zones of the British ‘Psammosteus’ Limestones Group and that the equivalent of the boundary between these zones should be placed either in, or just above, the primaeva Beds.     

THE GENETIC BASIS OF EVOLUTION OF THE MALE COURTSHIP SOUNDS IN THE DROSOPHILA VIRILIS GROUP

When courting, males of the Drosophila virilis group vibrate their wings and emit species-specific courtship sounds consisting of trains of polycyclic sound pulses. To analyze the genetic basis of evolutionary changes in the sounds we made an F₁ diallel set of reciprocal crosses between the members of the virilis phylad of the group (two stocks of D. virilis and one of D. americana americana, D. a. texana, D. novamexicana, and D. lummei). We also crossed the D. virilis stocks with the members of the montana phylad of the same group (D. kanekoi, D. littoralis, D. borealis, D. flavomontana, D. lacicola, and D. montana) and made a backcross (D. virilis x D. littoralis) x D. virilis using a D. virilis marker stock (b; sv t tb gp; cd; pe). The sounds of the hybrids were analyzed using the following parameters: the length of a pulse train (PTL), the number of pulses in a train (PN), the interpulse interval (IPI), the length of a pulse (PL), the number of cycles in a pulse (CN), and the length of a cycle (CL). In the virilis phylad, the differences between species appeared to be determined mainly by autosomal genes in each sound trait. The heritabilities (narrow-/broad-sense) obtained from the diallel tables were the following: PTL 0.662/0.817, PN 0.651/0.841, IPI 0.193/0.546, PL 0.408/0.552, CN 0.425/0.719, and CL 0.361/0.764. The direction of dominance is for longer PTL, higher PN and CN, and shorter IPI and CL. PL shows ambidirectional dominance. In the sounds of the virilis phylad species, PTL and PL seem to be phenotypically the most important parameters, since their components (PN and IPI for PTL, CN and CL for PL) are negatively correlated. In crosses between D. virilis and D. littoralis or D. flavomontana reciprocal hybrids differed from each other in PTL, IPI, PL, and CN indicating X-chromosomal or cytoplasmic inheritance. In the backcrosses between D. virilis and D. littoralis the role of the X chromosome was ascertained to be decisive. We conclude that an X-chromosomal major change allowing variation in IPI has occurred during the separation of the two D. virilis group phylads, the long IPI allowing variation also in PL (and CN). The evolution of the sounds in the virilis phylad has probably gone towards longer and denser pulse trains, while in the montana phylad the sounds have evolved in different directions.     

THE EFFECT OF ESTRADIOL ON THE CELL KINETICS IN THE UTERINE AND CERVICAL EPITHELIUM OF NEONATAL MICE

The effect of estradiol-17β on the length of the various phases of the cell cycle was studied in the neonatal mouse uterine, and cervical epithelium. A double labelling method was used, and in addition labelled mitoses were counted. In the uterus proper, estradiol shortens the length of the total cell cycle, T_c, from 17-9 hr to 15-7 hr, and the duration of S phase, T_s, from 6–7 to 5-1 hr 6 hr after estradiol treatment. 12 hr after estradiol treatment, T_c is shortened to 7-4 hr and T_s to 4–5 hr. The shortening of T_c at 12 hr is mainly due to an effect on T_G1, which is shortened from 8–55 hr in untreated animals to 1–8 hr in estradiol treated animals. The T_c of cervix epithelium cells in untreated animals was found to be 21-8 hr. After treating the mice for 6 hr with estradiol the T_c was now increased to 47 hr and further to 61 -2 hr following 12 hr treatment with the hormone. T_s increases from 8-3 hr to 15-2 hr following 6 hr estradiol treatment, and to 15-4 hr after 12 hr treatment. The effect is most pronounced in T_G1, which is lengthened from 10–95 hr in untreated animals to 28-1 hr and 43 hr, respectively, in animals treated for either 6 or 12 hr with estradiol.     

THE INTERTARSAL JOINT OF THE HARRIER-HAWKS POLYBOROIDES SPP. AND THE CRANE HAWK GERANOSPIZA CAERULESCENS

The Harrier-Hawks Polyboroides spp. and Crane Hawk Geranospiza caerulescens have legs capable of flexing backward as well as forward at the intertarsal joint. This is evidently adaptive to their habit of extracting prey from crevices using the feet. The structure and properties of the intertarsal joint have been investigated using an alcoholic specimen of P. typus and skeletons of P. typus and G. caerulescens; specimens of Circus cayaneus (alcoholic) and C. aeruginosus (skeleton) were used for comparison. Increased mobility of the joint extends to medial-lateral swing as well as flexion; the faculty for backward flexion appears to be somewhat offset by some loss of forward flexion. The tibiotarsus, tarsometatarsus and the ligaments and menisci of the intertarsal joint are described and figured. No significant differences in musculature were found. The most obvious difference between Circus on the one hand, and Polyboroides and Geranospiza on the other is the extreme narrowness of the joint in the latter two genera. This is probably the most important factor permitting increased joint mobility. The possible significance of other structural features observed is also discussed, and related adaptations of the distal tarsometatarsus and foot are pointed out. It is concluded that the resemblances of the hind limbs of these two genera are not strong evidence of phylogenetic relationship.     

MOLECULAR PHYLOGENY OF THE HAPTOPHYTA BASED ON THE rbcL GENE AND SEQUENCE VARIATION IN THE SPACER REGION OF THE RUBISCO OPERON

A phylogeny of 21 haptophyte algae was inferred by maximum parsimony, neighbor-joining, and maximum likelihood analyses of sequences of the plastid-encoded gene, rbcL. Sequence variation in the spacer region of the RUBISCO operon was also investigated. In all the rbcL trees constructed, the haptophytes form two distinct clades: one includes the Pavlovales and the other includes the Prymnesiales, Coccosphaerales, and Isochrysidales (all sensu Parke and Green 1976 . This relationship coincides with the recent taxonomic treatment splitting the division into two subclasses, the Prymnesidae and Pavlovidae ( Cavalier-Smith 1989 ) or the Prymnesiophycidae and the Pavlovophycidae using botanical suffixes ( Jordan and Green 1994 ), or into two classes, the Patelliferea and the Pavlovea ( Cavalier-Smith 1993 ). In the Prymnesiophycidae, all the coccolithophorids examined are placed in a single clade, which suggests a single origin of the coccolithophorids and the ability of coccolith formation in the haptophytes. The genus Chrysochromulina is polyphyletic. Species of Chrysochromulina with a very long haptonema and a compressed cell body (typical of species including the type C. parva Lackey) form a clade, including Imantonia, that is often classified in the Isochrysidales in the neighbor-joining tree, whereas some species possessing a nontypical cell body and cell covering form a clade with Prymnesium and Platychrysis in all trees. It is suggested that loss of the haptonema in Imantonia and the reduction in Prymnesium and Platychrysis occurred secondarily and independently in two different lineages. Within the coccolithophorids, four clades are recognized: Pleurochrysis, Calyptrosphaera-Cruciplacolithus-Calcidiscus-Umbilicosphaera, Helicosphaera, and Emiliania-Gephyrocapsa. A non-coccolith-bearing haptophyte, Isochrysis, is an ingroup of the Emiliania-Gephyrocapsa clade, suggesting its secondary loss of the ability to form a coccolith. Sequence comparison of the spacer region of RUBISCO operon supports most results obtained in the analysis of rbcL sequences. Monophyly of the Prymnesiales sensu Parke and Green is still unclear because of low (<50%) bootstrap support for this group.     

MOLECULAR EVIDENCE FOR THE ORIGIN OF THE S-DERIVED GENOMES OF POLYPLOID TRITICUM SPECIES

The genus Triticum includes several polyploid species that arose due to hybridization between two or more diploid species. Section Sitopsis is comprised of five diploid species given the genome designation S. Four polyploid species are recognized that contain an S or S-derived genome. We have used two repetitive DNA sequences found primarily in the S genomes of Triticum to determine the likely diploid progenitors of the polyploid species. Comparison of restriction fragments that hybridize to probes for these sequences suggests that T. speltoides is distinct from other members of section Sitopsis (i.e., T. longissimum, T. bicorne, T. searsii, and T. sharonense). The S-derived genome of T. aestivum is more closely related to T. speltoides than to the other Sitopsis diploids. The restriction fragment pattern of T. timopheevii is 98% identical to that of T. speltoides, while those of T. kotschyi and T. syriacum are identical to the group of diploids represented by T. longissimum, T. bicorne, T. searsii, and T. sharonense. Our results are compatible with previous molecular and biochemical data regarding relationships among Triticum species containing an S or S-derived genome.     

THE ZOOBENTHOS OF THE TOUW RIVER FLOODPLAIN

SUMMARY

An eighteen month study (January 1979 - June 1980) of the sediment, emergent and submerged plant-associated benthic macro-invertebrates of the western sector of the Touw River Flood-plain is reported for four study sites: Wilderness Lagoon, the Touw River and East and West Serpentine. Total invertebrate standing stocks on emergent vegetation were negligible. Those associated with submerged plant communities (essentially Potamogeton pectinatus L.), were generally very much greater than sediment-associated invertebrate standing stocks. The following mean biomasses were recorded for the study period: (a) sediments - Wilderness Lagoon, 19,25; Touw River, 13,06; West Serpentine, 4,37; East Serpentine, 4,68 g dry mass m^?2; (b) - Potamogeton-associated fauna - Touw River, 128,9; West Serpentine, 91; East Serpentine, 35,78 g dry mass m^?2. Of the 37 taxa recorded for Wilderness Lagoon, 33 for Touw River and 31 each for West and East Serpentine sites, the most consistently dominant species were the amphipods, Melita zeylanica Stebbing, Corophium triaenonyx Stebbing, and Grandidierella lignorum Barnard, the tanaid, Apseudes digitalis Brown, and the bivalve mollusc, Musculus virgiliae Barnard. However, suspension feeding Musculus was by far the most important taxon.

In addition to normal winter die-back of Potamogeton, submerged plant cutting activities in the Serpentine lead to an extended period of plant senescence, a prolonged recovery period at the West Serpentine station (between 4–5 months, compared with un-cut Touw River - approximately 2 months), almost complete failure of the plant at East Serpentine, and associated reductions or shifts in the invertebrate community standing stocks, composition and structure. Sediment-associated invertebrate standing stocks at both West and East Serpentine stations increased after Potamogeton harvesting, both immediately and in the long-term. However, these sediment fauna increases nowhere near compensated for the reductions of plant-associated faunal standing stocks due to cutting activities. The significance of these findings is discussed in terms of future management of the area, and comparisons with the situation in Swartvlei, where natural Potamogeton reduction occurred, and with other estuarine and coastal systems, are made.     

THE NATURAL AFFINITY OF THE CARBONIFEROUS SEED,CALLOSPERMARION

A seed of Callospermarion pusillum has been found attached to a long slender stalk. Significant features of the seed based upon additional specimens are described. Secretory cavities in the integument are similar to those in the stem of Callistophyton poroxyloides and the fertile foliage bearing the pollen organ Callandrium callistophytoides. Vesicaspora sp. pollen similar to that found in Callandrium, occurs in the pollen chamber. Callistophyton, Callandrium, and Callospermarion are considered to represent the detached organs of a new seed fern family, Callistophytaceae.     

THE EFFECT OF pH ON THE DIVISION RATE OF THE COCCOLITHOPHORID CRICOSPHAERA ELONGATA1

The division rate of Cricosphaera elongata was measured as a function of pH in a medium buffered with the CO₂-bicarbonate-carbonate system. The optimum pH for cell division of the coccolithophorid was 7.8. A change of the partial pressure of CO₂ in the medium from 0.03 to 5% did not affect the division rate. Between pH 6.4 and 7.8 changes in the bicarbonate concentration from 0.1 to 6.0 mm and carbonate concentration from 0.007 to 0.1 mm did not affect the rate of division. At loiv experimental pH, C. elongata was nonmotile and grew in clumps; at higher pH values, it was motile and solitary. Coccoliths were not found covering C. elongata if calcite was soluble in the medium.     

818. WISTERIA FLORIBUNDA WITH THE TORTUOUS TALE OF THE JAPANESE WISTERIA WITH THE LONGEST RACEMES

The history of the Japanese Wisteria with the longest racemes is fully investigated, with a review of all the names applied to it. The name W. macrobotrys is shown to belong to a different cultivar group now widely grown under the names ‘Burford’, ‘Honko’ and ‘Hocker Edge’. The new combination W. floribunda f. multijuga (Van Houtte) J. Compton & G. Thijsse is made in order to establish long‐term nomenclatural stability. A lectotype is designated for the hybrid name W. × formosa Rehder.     

THE ASSOCIATION BETWEEN THE POLYMORPHISMS AT THE Adh AND αGpdh LOCI AND THE In(2L)t INVERSION IN DROSOPHILA MELANOGASTER IN RELATION TO TEMPERATURE

Substantial allele-frequency changes were observed at the Adh and αGpdh loci in a seminatural population of Drosophila melanogaster kept in a tropical greenhouse during 1972–1985. Further analysis of the changes at the Adh and αGpdh loci showed that linkage disequilibrium between these loci occurred for a prolonged period due to the presence of In(2L)t, a long inversion on the left arm of the second chromosome. We observed increases in the frequencies of In(2L)t and of short inversions on the left arm of the second chromosome in subpopulations kept at 29.5°C or 33°C. These inversion-frequency increases were accompanied by an increase in Adh^s and a decrease in αGpdh^s frequency. In populations kept at 20°C and 25°C, inversion frequencies decreased, while αGpdh^s allele frequencies decreased at 25°C and increased at 20°C. At 33°C, egg-to-adult survival of individuals possessing In(2L)t, either in the homokaryotypic or the heterokaryotypic state, was higher than that of the other karyotypes of identical allozyme constitution (i.e., Adh^s αGpdh^F). Thus it seems that In(2L)t has a selective advantage at high temperature. We argue that the observed changes in allele frequencies at the Adh and αGpdh loci are, in part, due to genic selection and are not merely the result of selection acting on the chromosome rearrangements and hitchhiking of the allozymes. The results are discussed with respect to the latitudinal clines found for In(2L)t, Adh, and αGpdh.     

THE QUANTITATIVE ULTRASTRUCTURE OF THE MERISTEMATIC CELLS OF XANTHIUM STRUMARIUM DURING THE TRANSITION TO FLOWERING

Ultrastructural changes occurring in the central part of the apical meristem of the SDP Xanthium strumarium, induced to flower by a single 16-hr long night, were quantitatively investigated using stereological methods and compared to the changes previously reported in other species, particularly the LDP Sinapis alba. Changes detected in Xanthium, which are also found in other species, included: increase in cellular, cytoplasmic, cytoplasmic matrix and nucleolar sizes, change in nucleolar structure; increase in mitochondrial number and chondriome size, increase in dictyosome number. These changes are believed to be essential for floral evocation because of their universality. Other changes were specific to Xanthium and not detected in Sinapis. Accordingly, they were thought to be accompanying nonessential events of floral evocation in Xanthium. These changes included an increase in the number of plastid profiles and in plastidome size. The size of the nucleus, chromatin and vacuolar apparatus, as well as the number of vacuolar profiles, did not change in Xanthium, contrary to what was observed in other plants.