共查询到20条相似文献,搜索用时 15 毫秒
1.
We studied the reproductive ecology of Clintonia borealis, a clonal understory species, in eastern Ontario. Flowers are protogynous and require insect pollination for outcrossing and maximum seed set. Most pollination is done by nectar-gathering bumble bees. We recorded pollen deposition and flowering patterns in 1983. Flowering lasted for about 10 days in mid-June. Stigmatic pollen loads were lowest at the onset of blooming, reflecting the scarcity of male-phase flowers. Pollination increased significantly by peak bloom and subsequently remained high. However, since bees fly mostly between neighboring stems, much of the pollen transferred may have been geitonogamous. In 1983, seed set per flower was not increased by supplementing pollination or by reducing the number of fruits competing for resources per stem. Rather, flowers set more seeds (14%) only if both treatments were performed simultaneously. Seed size was increased by 25% when competitive fruits were removed and by 5% more when pollen was added under the removal treatment. Further work in 1984 showed that pollination effects may be related to changes in pollen source. Selfed flowers set fewer and smaller seeds than outcrossed ones when screened from insects and hand pollinated. Outcrossing distance had little effect on seed set or seed size. Thus, in C. borealis seed number and seed size are limited by a balance between maternal resource availability and the amount of outcrossing provided by pollinators. 相似文献
2.
The combined occurrence of both herkogamy and dichogamy in a hermaphrodite species has been considered to strongly favour outcrossing. In this study, we investigated in detail the reproductive ecology of Gentiana straminea Maxim. (Gentianaceae), a hermaphrodite perennial endemic to the Qinghai-Tibet Plateau. In a series of observations and experiments over four consecutive years, we examined whether the combination of dichogamy and herkogamy in individual flowers completely prevents geitonogamous pollen transfer in this species. The mode of floral development clearly indicates that autonomous self-pollination is completely avoided through herkogamy and dichogamy in individual flowers. This implication was confirmed by the breeding experiments, since no seed was produced when flowers were isolated. However, this gentian proved to be highly self-compatible when geitonogamous selfing was artificially induced. Many flowers opened simultaneously on individual plants, the ratio of male to female phase flowers was close to 2:1 in each inflorescence at the full anthesis phase, and they were randomly distributed amongst the upper, middle and lower parts of each stem's inflorescence. On average, Bombus sushikini Skorikov, the most frequent visitor and only legitimate pollinator of G. straminea, visited nearly two flowers per inflorescence, and four flowers per plant. Among the pollinators' foraging bouts, the proportions of geitonogamous visits to inflorescences or flowers within an individual plant were 29% and 37%, respectively. Therefore, despite the strict dichogamous and herkogamous characteristics of the individual flowers, geitonogamous selfing might still prevail in G. straminea because of the size of its floral displays and the continuous visiting behavior of B. sushkini. 相似文献
3.
Background and Aims
The number of flowers blooming simultaneously on a plant may have profound consequences for reproductive success. Large floral displays often attract more pollinator visits, increasing outcross pollen receipt. However, pollinators frequently probe more flowers in sequence on large displays, potentially increasing self-pollination and reducing pollen export per flower. To better understand how floral display size influences male and female fitness, we manipulated display phenotypes and then used paternity analysis to quantify siring success and selfing rates.Methods
To facilitate unambiguous assignment of paternity, we established four replicate (cloned) arrays of Mimulus ringens, each consisting of genets with unique combinations of homozygous marker genotypes. In each array, we trimmed displays to two, four, eight or 16 flowers. When fruits ripened, we counted the number of seeds per fruit and assigned paternity to 1935 progeny.Key Results
Siring success per flower declined sharply with increasing display size, while female success per flower did not vary with display. The rate of self-fertilization increased for large floral displays, but siring losses due to geitonogamous pollen discounting were much greater than siring gains through increased self-fertilization. As display size increased, each additional seed sired through geitonogamous self-pollination was associated with a loss of 9·7 seeds sired through outcrossing.Conclusions
Although total fitness increased with floral display size, the marginal return on each additional flower declined steadily as display size increased. Therefore, a plant could maximize fitness by producing small displays over a long flowering period, rather than large displays over a brief flowering period. 相似文献4.
In animal‐pollinated plants, both the spatial distribution of flowering individuals and the number of flowers that an individual displays affect pollen deposition rates and female reproductive success. Heterostylous species are likely to be particularly sensitive to the contingencies of spatial distribution, as they are reproductively subdivided into distinct mating groups, which usually exhibit self‐ and intra‐morph incompatibility and differ in floral morphology. In this paper, we explore the joint effects of both spatial distribution of potential mates and floral display size on morph‐specific pollen deposition rates and seed set patterns in two natural populations of Pulmonaria officinalis, a distylous species with a weak self‐incompatibility system. Both total stigmatic pollen load and the proportion of legitimate pollen decreased with increasing spatial isolation. Legitimate (intermorph) pollen transfer was, however, asymmetric and decreased more rapidly with decreasing proximity to a compatible legitimate mating partner in the S‐morph than in the L‐morph. Total stigmatic pollen loads per flower increased with increasing floral display size, indicating that large plants are disproportionately more visited than smaller individuals. However, because legitimate pollen deposition decreased with increasing floral display size, these results also suggest that larger numbers of flowers increase the degree of geitonogamous pollination. In both the L‐ and S‐morph, seed set significantly decreased with increasing isolation from a legitimate mating partner, but in the L‐morph seed set was less dependent on the spatial distribution of the S‐morph. In addition, seed set significantly increased with floral display size in the L‐morph, but not in the S‐morph. These findings indicate that the spatial distribution of potential mates and variation in floral display size may cause morph‐specific differences in pollen deposition rates and female reproductive success. 相似文献
5.
Alexander F. Motten 《American journal of botany》1982,69(8):1296-1305
Hepatica americana is one of the earliest flowering vernal herbs in the deciduous forests of piedmont North Carolina. Unlike most other members of the spring wildflower community, its flowers are nectarless and autogamous. The number of insect visits a flower receives varies markedly through the blooming season and is lowest when H. americana is in full bloom. Potential for outcrossing as measured by the seed set of emasculated flowers follows the same pattern. The mid-season decline in insect-pollination is most likely the result of competition with Erythronium umbilicatum, which flowers concurrently with H. americana and is much more attractive to the solitary bees that are the principal early season pollen vectors. Because of this competitive disadvantage, seed set of H. americana in piedmont North Carolina would be pollination-limited if the flowers were not autogamous. Autogamy may also be favored by flowering early in the spring before pollinators are available. Opportunities for outcrossing in H. americana are primarily the result of protogyny and blooming earlier than competitors. 相似文献
6.
To determine the breeding system of Lycium cestroides,several treatments were performed: self-, cross-, and geitonogamous pollinations, autonomous self-pollination, and a control
(flowers exposed to natural pollinators). Production and quality of fruits and seeds as well as pollen tube growth were evaluated
for each treatment. Experimental pollinations indicate that L. cestroides is self-incompatible since fruits were obtained only under cross-, and open-pollination treatments. However, in self- and
geitonogamous hand pollinations, as well as in autonomous self-pollination, pollen tubes developed successfully and reached
the ovules. The speed of pollen tube growth did not differ significantly among the different hand-pollinated flowers (cross-,
self-, and geitonogamous). These facts indicate the presence of an ovarian self-incompatibility system. Significant differences
were observed in fruit set, fruit size, and seed number per fruit between cross-pollinated flowers and open pollination (control).
These results could be explained in terms of quantity and quality of pollination in each case.
Received: 2 November 2000 / Revision accepted: 26 January 2001 相似文献
7.
M. E. Griffiths Z. Tsvuura D. C. Franklin M. J. Lawes 《Plant biology (Stuttgart, Germany)》2010,12(3):495-502
Synchronous monocarpy in long‐lived plants is often associated with pollination by wind, in part because infrequent mass flowering may satiate pollinators. Selfing in synchronous monocarps may provide reproductive assurance but conflict with the benefits of outcrossing, a key evolutionary driver of synchrony. We predicted that animal‐pollinated species with synchronous flowering would have unspecialised flowers and attract abundant generalised pollinators, but predictions for selfing and outcrossing frequencies were not obvious. We examined the pollination biology of Isoglossa woodii (Acanthaceae), an insect‐pollinated, monocarpic herb that flowers synchronously at 4–7‐year intervals. The most frequent visitor to I. woodii flowers was the African honeybee, Apis mellifera adansonii. Hand‐pollination failed to enhance seed production, indicating that the pollinators were not saturated. No seed was set in the absence of pollinators. Seed set was similar among selfed and outcrossed flowers, demonstrating a geitonogamous mixed‐mating strategy with no direct evidence of preferential outcrossing. Flowers contained four ovules, but most fruits only developed one seed, raising the possibility that preferential outcrossing occurs by post‐pollination processes. We argue that a number of the theoretical concerns about geitonogamous selfing as a form of reproductive assurance do not apply to a long‐lived synchronous monocarp such as I. woodii. 相似文献
8.
A. -L. Jacquemart 《Plant Systematics and Evolution》1997,207(3-4):159-172
The reproductive success of three co-flowering species ofVaccinium (V. myrtillus, V. vitis-idaea andV. uliginosum) was studied in one heathland of the Upper Ardennes, Belgium, during three years (1988–1990). The purpose was to examine whether pollen limitation, flower position and flowering phenology may influence patterns of fruit set in these three sympatric species. I quantified fruit and seed set following supplementary hand-pollinations and compared this to natural fruit set. On the same plants, I also quantified fruit and seed set in relation to the spatial position of the flowers on the ramet and their temporal sequence of blooming. Hand-pollination had no significant effect on fruit set inV. vitis-idaea andV. uliginosum, but significantly increased seed number per fruit in 1989. InV. myrtillus both fruit and seed set were increased by supplementary pollination, but significantly in only one year. Analyses of position effects revealed that the fruits in the lower positions in the ramet did not mature preferentially and did not contain more seeds inV. uliginosum and inV. vitis-idaea. Flowering phenology also had no significant effect for these species. InV. myrtillus hand-pollinated flowers showed a seasonal decline in seed number, and control (naturally pollinated) flowers showed a seasonal increase in fruit set. Fruit and seed set appear to be pollen-limited rather than resource-limited inV. uliginosum. In the two other species, unfavorable weather (frosts) could be a more important cause of low fruit and seed set. 相似文献
9.
P. Garcia‐Fayos M. C. Castellanos J. G. Segarra‐Moragues 《Plant biology (Stuttgart, Germany)》2018,20(3):627-635
- Self‐pollination by geitonogamy is likely in self‐compatible plants that simultaneously expose a large number of flowers to pollinators. However, progeny of these plants is often highly allogamous. Although mechanisms to increase cross‐pollination have been identified and studied, their relative importance has rarely been addressed simultaneously in plant populations.
- We used Rosmarinus officinalis to explore factors that influence the probability of self‐fertilisation due to geitonogamy or that purge its consequences, focusing on their effects on seed germination and allogamy rate. We experimentally tested the effect of geitonogamy on the proportion of filled seeds and how it influences germination rate. During two field seasons, we studied how life history and flowering traits of individuals influence seed germination and allogamy rates of their progeny in wild populations at the extremes of the altitudinal range. The traits considered were plant size, population density, duration of the flowering season, number of open flowers, flowering synchrony among individuals within populations and proportion of male‐sterile flowers.
- We found that most seeds obtained experimentally from self‐pollination were apparently healthy but empty, and that the proportion of filled seeds drove the differences in germination rate between self‐ and cross‐pollination experiments. Plants from wild populations consistently had low germination rate and high rate of allogamy, as determined with microsatellites. Germination rate related positively to the length of the flowering season, flowering synchrony and the ratio of male‐sterile flowers, whereas the rate of allogamous seedlings was positively related only to the ratio of male‐sterile flowers.
- Rosemary plants purge most of the inbreeding caused by its pollination system by aborting the seeds. This study showed that the rates of seed germination and allogamy of the seedlings depend on a complex combination of factors that vary in space and time. Male sterility of flowers, length of the flowering season and flowering synchrony of individuals within populations all favour high rates of cross‐pollination, therefore increasing germination and allogamy rates. Flowering traits appear to be highly plastic and respond to local and seasonal conditions.
10.
Reproductive success of Calopogon tuberosus, which produces no nectar, was investigated in relation to inflorescence size and dispersion pattern. Mean inflorescence size was 2.56 (range 1–10). A bagging experiment showed that insects are required for pollen transfer and that fruits are produced from self-, geitonogamous, and cross-pollinations; fruit set was not 100%. Fruit set of nonmanipulated plants was limited by the number of pollinator visits. Reproductive success increased with increasing inflorescence size, although not above theoretical predictions. However, the probability of producing no fruit or contributing no pollinia decreased with increasing inflorescence size since sequential flowering increased the probability of a pollinator visit to the inflorescence over the blooming period. Large inflorescences did not provide a greater pollinator attraction than small ones, because inflorescences only presented a few open flowers at a time. In addition, flowers on plants growing in clumps of 2–8 plants had a higher probability of setting fruit, apparently because of increased pollinator attraction. Although there are obvious selective advantages for large inflorescences, the sequential flowering habit, and low resource availability may reduce the advantages of large inflorescence size at our study site. 相似文献
11.
Javier Herrera 《American journal of botany》1991,78(6):789-794
Patterns of fruit set were studied in Lavandula stoechas, a Mediterranean shrub commonly occurring in southern Spain. The small, hermaphroditic flowers of this shrub are aggregated into dense, headlike inflorescences and exhibit extensive variations in fecundity. It was shown that as the number of developing fruits in the inflorescence increased, the probability of a flower setting fruit, the size of seeds, and their germinability decreased, most likely because of strong within-inflorescence resource limitation. An experiment was designed to ascertain whether increased fertility in late-opening flowers could be induced through reallocation of reproductive resources between different inflorescences. The experiment consisted of removing half of the inflorescence buds from a set of plants and comparing their fecundity with that of intact individuals. Thinning did not increase the proportion of flowers setting fruit which, in fact, was slightly lower than that of intact individuals (probably due to some reduction of floral display brought about by thinning). Although treated plants produced heavier seeds than controls, results suggest that inflorescences of L. stoechas behave as autonomous modules among which resources cannot be reallocated. Predispersal seed predation by insects accounted on average for a 31% reduction in fruit set. Predation was found to be nonrandomly distributed within inflorescences, with most damage concentrated on late fruits (i.e., those with smaller and less germinable seeds). 相似文献
12.
Erigeron glabellus and Aster sibiricus have similar flowers, share pollinators, but bloom sequentially in interior Alaska. Both species depend on insect pollination for seed set: the Erigeron is self-incompatible, and the Aster is apparently self-compatible but allogamous. To test the hypothesis that sequential blooming is maintained by natural selection generated by reproductive interference, we manipulated the flowering time of Erigeron, forcing it to bloom simultaneously with Aster, and measured female fecundity in both species. We found no evidence of reduced female fecundity in either species caused by the presence of the sympatric “competitor” or by artificial pollination with the heterospecific pollen prior to conspecific pollination. Two-species mixtures of simultaneously blooming Aster and Erigeron experienced significant interspecific visitation, which may, under natural conditions, cause loss of pollen to alien stigmas and depressed male fecundity, at least in Erigeron. We found no evidence that sequential blooming in Erigeron and Aster is maintained by depressed female fecundity through pollinator sharing. If sequential blooming is maintained by natural selection, it seems more likely to be the result of selection generated by depressed male fitness through pollen loss to alien stigmas. 相似文献
13.
James D. Thomson 《American journal of botany》1985,72(5):737-740
Ovule development in Diervilla lonicera (Caprifoliaceae) is limited by insufficient pollination early in the blooming period and during extensive rainy periods. Production of flowers is skewed in time; an initial burst of flowering is followed by a long period of sparse flower production. Ovule number per flower increases through the blooming period. I discuss the interactions of fruit and seed set, ovule number, and bumble bee pollinator visitation patterns. When certain flowers have a higher a priori probability of successful pollination, it may be advantageous for plants to put more ovules in those flowers. Selective ovule deployment may be a general adaptive phenomenon that has received little attention. 相似文献
14.
Summary As in many plant species, Lomatium salmoniflorum (Umbelliferae) individuals produce many flowers, only a subset of which produce mature seeds that escape seed parasitism and enter the seed bank. The interrelationships between the timing and number of flowers produced, sex expression, seed set, and seed parasitism were studied for their direct and indirect effects on the numbers and masses of viable seeds produced by individual plants. In a sample population of 369 plants that produced 161 386 flowers, 76% of the plants produced some hermaphroditic flowers. The percentage of hermaphroditic flowers increased significantly with the total number of flowers produced by a plant. Seed set was 65–90% in plants producing >600 flowers, but was highly variable in plants producing fewer flowers. Hand-pollinated plants showed the same pattern of seed set, suggesting that variable seed set in small plants may result from insufficient resources for seed development. The majority of schizocarps was produced by only 12% of the plants. Parasites killed 24.5% of the seeds prior to dispersal. Another 14.5% of the seeds lacked endosperm. Hence, the initial 161 386 flowers, which included 25874 hermaphroditic flowers each capable of producing two seeds, produced 42 468 seeds of which an estimated 25906 entered the seed bank as undamaged seeds with fully developed endosperm. Path analysis indicated that the number of hermaphroditic flowers on a plant and the percentage of seeds attacked by seed parasites had the greatest direct effects on the number of viable seeds entering the seed bank. The date at which a plant began flowering and the percentage of flowers setting seed had smaller or only indirect effects on viable seed production. Mean seed mass for plants was not significantly related to any of the factors that affected seed number, but little of the variance in seed mass occurred among plants. Masses of intact seeds in the population ranged 9-fold in both 1987 and 1988. Thirty-five percent of the variance was among seeds within umbels, 46% was among umbels within plants, and only 19% was among plants. The large variation among umbels within plants resulted from a seasonal pattern in which seeds from umbels produced late in the spring had lower mean seed masses than seeds from umbels produced early in the spring. Overall, the results indicate that both direct and indirect interactions between number of flowers, the date of initiation of flowering, seed set, and seed parasitism affect the number of viable seeds entering the seed bank. These interactions strongly bias viable seed output to a small minority of plants that produce many seeds with a wide range of masses over the growing season. 相似文献
15.
Robert Ornduff 《Plant Systematics and Evolution》1985,149(3-4):299-302
A population ofWurmbea dioica subsp.alba in Western Australia contained monoclinous and andromonoecious individuals in roughly equal proportions. The average number of flowers per inflorescence for the former was 2.6 and for the latter 2.9, with its terminal flower staminate. Ovule number and seed production per perfect flower of both morphs decreased progressively from lower to upper flowers in the inflorescence. Two-flowered monoclinous individuals had a greater percentage of ovules maturing to seed than did threeflowered ones, but seed production of the latter exceeded that of the former. No differences in percent seed set or in seed production were noted for similar individuals of andromonoecious individuals. Although monoclinous and andromonoecious plants apparently contributed equally to the pollen pool, seed production of the former exceeded that of the latter. Thus, the energetic costs of monocliny exceed those of andromonoecy in this population. 相似文献
16.
Clemens Schlindwein Christian Westerkamp Airton Torres Carvalho Paulo Milet‐Pinheiro 《Botanical journal of the Linnean Society. Linnean Society of London》2014,176(3):396-407
Mass flowering is a widespread blooming strategy among Neotropical trees that has been frequently suggested to increase geitonogamous pollination. We investigated the pollination ecology of the mass‐flowering tree Handroanthus impetiginosus, addressing its breeding system, the role in pollination of different visitors, the impact of nectar robbers on fruit set and the function of colour changes in nectar guides. This xenogamous species is mainly pollinated by Centris and Euglossa bees (Apidae) seeking nectar, which are known to fly long distances. The flowers favour these bees by having: (1) a closed entrance in newly opened flowers which provides access only to strong bees capable of deforming the flower tube; and (2) a nectar chamber that is accessible only to long‐tongued bees. Only first‐day flowers with yellow nectar guides produce nectar. Pollinators prefer these flowers over second‐ and third‐day flowers with orange and red nectar guides, respectively. Nectar robbers damage two‐thirds of the flowers and this robbing activity decreases fruit set by half. We attribute the low fruit set of H. impetiginosus to the intense nectar robbing and hypothesize that visual signalling of nectar presence in newly opened (receptive) flowers reduces geitonogamy by minimizing bee visits to unrewarding (non‐receptive) flowers. © 2014 The Linnean Society of London, Botanical Journal of the Linnean Society, 2014, 176 , 396–407. 相似文献
17.
Sexual dimorphism and fruit production in a dioecious understory tree,Ilex opaca Ait. 总被引:1,自引:0,他引:1
D. E. Carr 《Oecologia》1991,85(3):381-388
Summary This study suggested that sexual selection is potentially an important factor in the maintenance of dioecy in the American holly, Ilex opaca (Aquifoliaceae). Sexual dimorphisms in flower production and phenology were highly significant in this understory tree. On average, individual males produced 7.4 times as many flowers as did female trees. Staminate flowers lasted only a single day, whereas pistillate flowers lasted 3–4 days, during which they showed no significant decline in their ability to produce fruit after pollination. Individual male trees opened their flower buds asynchronously during the season, maximizing the number of days they were in flower. Individual females opened their buds more synchronously, maximizing their floral display at one point in time. Females produced fruits in numbers that were somewhat less than proportional to their flower production. Fruit development was initiated from only 38.9% and 69.5% of pistillate flowers in 1987 and 1988, respectively. By the time of ripening, an average female had lost 62.3%, 24.3%, and 11.1% of its initial fruit crop in 1986, 1987, and 1988, respectively. The proportion of fruit lost in 1986 was independent of the number of fruit that initially began development. In 1988, artifically supplementing pollen to a large number of flowers failed to increase either fruit or seed production relative to control branches with unsupplemented flowers. This suggested that resource levels were likely more important than pollen availability in limiting female reproductive success. These observations on I. opaca were consistent with the expectations for a population in which male reproductive success continues to benefit from continued pollinator service and female reproductive success does not. 相似文献
18.
Opportunities for selfing through geitonogamy are possible if more than one flower within the same clone, inflorescence, or floral unit is open at the same time. In a total of 200 inflorescences in two natural populations of Iris versicolor, flowers were observed and classified daily on the basis of anther dehiscence and stigma receptivity. Analysis of the flowering phenology demonstrated that (1) protandry limits opportunities for autogamy, (2) flowers mature sequentially within a floral unit (defined as a cluster of flowers borne on a single branch within an inflorescence), preventing the opportunity for geitonogamous fertilization between flowers of the same floral unit, and (3) 77% of all flowers had no opportunity to be pollinated by another flower within the same inflorescence. Both the number and the proportion of flowers with opportunities for geitonogamy increased with the number of flowers open in each population, and opportunities for geitonogamy also increased with the number of floral units within inflorescences. These morphological and phenological controls suggest that when selfing occurs in this species, it is most likely to occur between flowers on different inflorescences within the same clone. Since the organization of whole inflorescences in space is determined primarily by rhizome placement, clonal architecture may play an important role in mating system regulation in this species. 相似文献
19.
S. K. Emms 《American journal of botany》1993,80(8):914-923
I describe patterns of sex allocation and gamete packaging in the andromonoecious lily Zigadenus paniculatus. In this species, pistil length was continuously, but bimodally, distributed within plants, and smaller pistils contained fewer mature ovules. In hermaphrodite flowers, ovule number per flower increased with blooming rank in small plants but decreased with blooming rank in large plants. Flowers with pistils less than three-fourths stamen length almost never produced fruits and were classified as males. The pedicel, tepals, stamens, and pistil of hermaphrodite flowers were all heavier than those of males. Hermaphrodite flowers were concentrated on the terminal raceme, males on the lower racemes. In combination with acropetal blooming, this spatial separation of flower types resulted in a seasonal decline in the proportion of open flowers that were hermaphrodite. However, individual flowers were protandrous, so that the population sex ratio, initially strongly male-biased, declined as the season progressed. Hand pollinations showed that plants were self-incompatible. Inflorescence size was positively correlated with bulb size, and plants with large inflorescences had a higher proportion of male flowers. Nutrient supplementation had no effect on inflorescence size, but increased the proportion of hermaphrodite flowers. Nutrient-supplemented plants also began blooming earlier than controls. I discuss these patterns in relation to the adaptive significance of andromonoecious breeding systems. 相似文献
20.
Sources of variation in pollinator contribution within a guild: the effects of plant and pollinator factors 总被引:3,自引:0,他引:3
Among plants visited by many pollinator species, the relative contribution of each pollinator to plant reproduction is determined by variation in both pollinator and plant traits. Here we evaluate how pollinator movement among plants, apparent pollen carryover, ovule number, resource limitation of seed set, and pollen output affect variation in contribution of individual pollinator species to seed set in Lithophragma parviflorum (Saxifragaceae), a species visited by a broad spectrum of visitors, including beeflies, bees and a moth species. A previous study demonstrated differences among visitor species in their single-visit pollination efficacy but did not evaluate how differences in visitation patterns and pollen carryover affect pollinator efficacy. Incorporation of differential visitation patterns and pollen carryover effects —commonly cited as potentially important in evaluating pollinator guilds — had minor effects (0–0.6% change) on the estimates of relative contribution based on visit frequency and single-visit efficacy alone. Beeflies visited significantly more flowers per inflorescence than the bees and the moth. Seed set remained virtually constant during the first three visited flowers for beeflies and larger bees, indicating that apparent pollen carryover did not reduce per-visit efficacy of these taxa. In contrast, Greya moth visits showed a decrease in seed set by 55.4% and the smaller bees by 45.4% from first to second flower. The larger carryover effects in smaller bees and Greya were diminished in importance by their small overall contribution to seed set. Three variable plant traits may affect seed set: ovule number, resource limitation on seed maturation, and pollen output. Ovule number per flower declined strongly with later position within inflorescences. Numbers were much higher in first-year greenhouse-grown plants than in field populations, and differences increased during 3 years of study. Mean pollen count by position varied 7-fold among flowers; it paralleled ovule number variation, resulting in a relatively stable pollen:ovule ratio. Resource limitation of seed set increased strongly with later flowering, with seed set in hand-pollinated flowers ranging from 66% in early flowers to 0% in the last two flowers of all plants. Variation in ovule number and resource limitation of seed maturation jointly had a strong effect on the number of seeds per flower. Visitation to early flowers had the potential to cause more seed set than visitation to later flowers. Overall, the most important sources of variation to seed production contribution were differences among pollinators in abundance and absolute efficacy (ovules fertilized on a single visit) and potentially differential phenology among visitor species. These effects are likely to vary among populations and years. 相似文献